Bismark

Bismark Processing Report

zr2096_4_s1_R1_val_1.fq.gz and zr2096_4_s1_R2_val_2.fq.gz

Data processed at 13:52 on 2018-04-27


Alignment

Sequence pairs analysed in total 1,000,000
Paired-end alignments with a unique best hit 541,137
Pairs without alignments under any condition 234,921
Pairs that did not map uniquely 223,942
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 54.1 %Unique AlignmentsUnique Alignments: 54.1 %: 23.5 %No AlignmentNo Alignment: 23.5 %: 22.4 %Multiple AlignmentsMultiple Alignments: 22.4 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 13,656,928
Methylated C's in CpG context 1,167,119
Methylated C's in CHG context 68,888
Methylated C's in CHH context 408,851
Methylated C's in Unknown context 37,082
Unmethylated C's in CpG context 579,377
Unmethylated C's in CHG context 2,907,466
Unmethylated C's in CHH context 8,525,227
Unmethylated C's in Unknown context 172,449
Percentage methylation (CpG context) 66.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 4.6%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 137,788 original top strand
CTOT 132,258 complementary to original top strand
CTOB 133,147 complementary to original bottom strand
OB 137,944 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB025k50k75k100k125k150k

Deduplication

Alignments analysed 541,137
Unique alignments 540,354
Duplicates removed 783
Duplicated alignments were found at 737 different positions
Created with Highcharts 5.0.5: 99.9 %UniqueUnique: 99.9 %: 0.1 %DuplicateDuplicate: 0.1 %

Cytosine Methylation after Extraction

Total C's analysed 8,656,657
Methylated C's in CpG context 729,965
Methylated C's in CHG context 45,672
Methylated C's in CHH context 245,765
Unmethylated C's in CpG context 371,588
Unmethylated C's in CHG context 1,834,338
Unmethylated C's in CHH context 5,429,329
Percentage methylation (CpG context) 66.3%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 4.3%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls051015202530354045505560657075800102030405060708090100010k20k30k40k50k60k70k80k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253035404550556065707580010203040506070809010005k10k15k20k25k30k35k40k45k50k