Bismark

Bismark Processing Report

zr2096_5_s1_R1_val_1.fq.gz and zr2096_5_s1_R2_val_2.fq.gz

Data processed at 13:52 on 2018-04-27


Alignment

Sequence pairs analysed in total 1,000,000
Paired-end alignments with a unique best hit 520,106
Pairs without alignments under any condition 278,464
Pairs that did not map uniquely 201,430
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 52.0 %Unique AlignmentsUnique Alignments: 52.0 %: 27.8 %No AlignmentNo Alignment: 27.8 %: 20.1 %Multiple AlignmentsMultiple Alignments: 20.1 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 13,613,606
Methylated C's in CpG context 1,168,935
Methylated C's in CHG context 74,696
Methylated C's in CHH context 406,740
Methylated C's in Unknown context 40,389
Unmethylated C's in CpG context 543,270
Unmethylated C's in CHG context 2,814,533
Unmethylated C's in CHH context 8,605,432
Unmethylated C's in Unknown context 174,268
Percentage methylation (CpG context) 68.3%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 4.5%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 134,098 original top strand
CTOT 126,227 complementary to original top strand
CTOB 126,218 complementary to original bottom strand
OB 133,563 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB025k50k75k100k125k150k

Deduplication

Alignments analysed 520,106
Unique alignments 518,859
Duplicates removed 1,247
Duplicated alignments were found at 1147 different positions
Created with Highcharts 5.0.5: 99.8 %UniqueUnique: 99.8 %: 0.2 %DuplicateDuplicate: 0.2 %

Cytosine Methylation after Extraction

Total C's analysed 8,805,499
Methylated C's in CpG context 743,249
Methylated C's in CHG context 49,454
Methylated C's in CHH context 250,635
Unmethylated C's in CpG context 355,669
Unmethylated C's in CHG context 1,807,852
Unmethylated C's in CHH context 5,598,640
Percentage methylation (CpG context) 67.6%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 4.3%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls051015202530354045505560657075800102030405060708090100010k20k30k40k50k60k70k80k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253035404550556065707580010203040506070809010005k10k15k20k25k30k35k40k45k50k55k