Bismark report for: zr2096_5_s1_R1_val_1.fq.gz and zr2096_5_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000000 Number of paired-end alignments with a unique best hit: 520106 Mapping efficiency: 52.0% Sequence pairs with no alignments under any condition: 278464 Sequence pairs did not map uniquely: 201430 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 134098 ((converted) top strand) GA/CT/CT: 126227 (complementary to (converted) top strand) GA/CT/GA: 126218 (complementary to (converted) bottom strand) CT/GA/GA: 133563 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 13613606 Total methylated C's in CpG context: 1168935 Total methylated C's in CHG context: 74696 Total methylated C's in CHH context: 406740 Total methylated C's in Unknown context: 40389 Total unmethylated C's in CpG context: 543270 Total unmethylated C's in CHG context: 2814533 Total unmethylated C's in CHH context: 8605432 Total unmethylated C's in Unknown context: 174268 C methylated in CpG context: 68.3% C methylated in CHG context: 2.6% C methylated in CHH context: 4.5% C methylated in unknown context (CN or CHN): 18.8% Bismark completed in 0d 0h 9m 58s