Bismark

Bismark Processing Report

zr2096_6_s1_R1_val_1.fq.gz and zr2096_6_s1_R2_val_2.fq.gz

Data processed at 13:52 on 2018-04-27


Alignment

Sequence pairs analysed in total 1,000,000
Paired-end alignments with a unique best hit 545,007
Pairs without alignments under any condition 227,088
Pairs that did not map uniquely 227,905
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 54.5 %Unique AlignmentsUnique Alignments: 54.5 %: 22.7 %No AlignmentNo Alignment: 22.7 %: 22.8 %Multiple AlignmentsMultiple Alignments: 22.8 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 13,938,301
Methylated C's in CpG context 1,349,757
Methylated C's in CHG context 71,272
Methylated C's in CHH context 402,359
Methylated C's in Unknown context 39,066
Unmethylated C's in CpG context 478,109
Unmethylated C's in CHG context 2,963,255
Unmethylated C's in CHH context 8,673,549
Unmethylated C's in Unknown context 179,065
Percentage methylation (CpG context) 73.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 4.4%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 142,505 original top strand
CTOT 130,385 complementary to original top strand
CTOB 129,517 complementary to original bottom strand
OB 142,600 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB025k50k75k100k125k150k

Deduplication

Alignments analysed 545,007
Unique alignments 544,187
Duplicates removed 820
Duplicated alignments were found at 778 different positions
Created with Highcharts 5.0.5: 99.8 %UniqueUnique: 99.8 %: 0.2 %DuplicateDuplicate: 0.2 %

Cytosine Methylation after Extraction

Total C's analysed 8,865,699
Methylated C's in CpG context 848,156
Methylated C's in CHG context 47,159
Methylated C's in CHH context 244,710
Unmethylated C's in CpG context 307,232
Unmethylated C's in CHG context 1,874,872
Unmethylated C's in CHH context 5,543,570
Percentage methylation (CpG context) 73.4%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 4.2%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls051015202530354045505560657075800102030405060708090100010k20k30k40k50k60k70k80k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253035404550556065707580010203040506070809010005k10k15k20k25k30k35k40k45k50k55k