Bismark

Bismark Processing Report

zr2096_7_s1_R1_val_1.fq.gz and zr2096_7_s1_R2_val_2.fq.gz

Data processed at 13:52 on 2018-04-27


Alignment

Sequence pairs analysed in total 1,000,000
Paired-end alignments with a unique best hit 518,103
Pairs without alignments under any condition 286,502
Pairs that did not map uniquely 195,395
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 51.8 %Unique AlignmentsUnique Alignments: 51.8 %: 28.7 %No AlignmentNo Alignment: 28.7 %: 19.5 %Multiple AlignmentsMultiple Alignments: 19.5 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 13,806,945
Methylated C's in CpG context 1,263,772
Methylated C's in CHG context 78,374
Methylated C's in CHH context 384,212
Methylated C's in Unknown context 36,797
Unmethylated C's in CpG context 483,667
Unmethylated C's in CHG context 2,916,755
Unmethylated C's in CHH context 8,680,165
Unmethylated C's in Unknown context 171,742
Percentage methylation (CpG context) 72.3%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 4.2%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 131,416 original top strand
CTOT 127,670 complementary to original top strand
CTOB 127,990 complementary to original bottom strand
OB 131,027 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB025k50k75k100k125k150k

Deduplication

Alignments analysed 518,103
Unique alignments 517,334
Duplicates removed 769
Duplicated alignments were found at 751 different positions
Created with Highcharts 5.0.5: 99.9 %UniqueUnique: 99.9 %: 0.1 %DuplicateDuplicate: 0.1 %

Cytosine Methylation after Extraction

Total C's analysed 9,248,047
Methylated C's in CpG context 833,778
Methylated C's in CHG context 54,322
Methylated C's in CHH context 246,563
Unmethylated C's in CpG context 327,574
Unmethylated C's in CHG context 1,940,270
Unmethylated C's in CHH context 5,845,540
Percentage methylation (CpG context) 71.8%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 4.0%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls051015202530354045505560657075800102030405060708090100010k20k30k40k50k60k70k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253035404550556065707580010203040506070809010005k10k15k20k25k30k35k40k45k50k55k