Bismark

Bismark Processing Report

/Volumes/Serine/wd/18-04-27/zr2096_10_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_10_s1_R2_val_2.fq.gz

Data processed at 18:08 on 2018-04-29


Alignment

Sequence pairs analysed in total 17,448,883
Paired-end alignments with a unique best hit 9,282,636
Pairs without alignments under any condition 4,524,985
Pairs that did not map uniquely 3,641,262
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 53.2 %Unique AlignmentsUnique Alignments: 53.2 %: 25.9 %No AlignmentNo Alignment: 25.9 %: 20.9 %Multiple AlignmentsMultiple Alignments: 20.9 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 252,303,007
Methylated C's in CpG context 24,797,899
Methylated C's in CHG context 1,458,878
Methylated C's in CHH context 6,698,414
Methylated C's in Unknown context 649,565
Unmethylated C's in CpG context 10,526,009
Unmethylated C's in CHG context 54,477,988
Unmethylated C's in CHH context 154,343,819
Unmethylated C's in Unknown context 2,848,039
Percentage methylation (CpG context) 70.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 4.2%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 2,365,261 original top strand
CTOT 2,269,368 complementary to original top strand
CTOB 2,275,104 complementary to original bottom strand
OB 2,372,903 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB0500k1 000k1 500k2 000k2 500k

Deduplication

Alignments analysed 9,282,636
Unique alignments 8,959,633
Duplicates removed 323,003
Duplicated alignments were found at 273923 different positions
Created with Highcharts 5.0.5: 96.5 %UniqueUnique: 96.5 %: 3.5 %DuplicateDuplicate: 3.5 %

Cytosine Methylation after Extraction

Total C's analysed 162,550,688
Methylated C's in CpG context 15,623,053
Methylated C's in CHG context 964,793
Methylated C's in CHH context 4,172,504
Unmethylated C's in CpG context 6,899,821
Unmethylated C's in CHG context 34,745,591
Unmethylated C's in CHH context 100,144,926
Percentage methylation (CpG context) 69.4%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 4.0%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000200k400k600k800k1 000k1 200k1 400k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000100k200k300k400k500k600k700k800k900k1 000k