Bismark

Bismark Processing Report

/Volumes/Serine/wd/18-04-27/zr2096_1_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_1_s1_R2_val_2.fq.gz

Data processed at 18:08 on 2018-04-29


Alignment

Sequence pairs analysed in total 28,603,346
Paired-end alignments with a unique best hit 8,273,829
Pairs without alignments under any condition 17,321,484
Pairs that did not map uniquely 3,008,033
Genomic sequence context not extractable (edges of chromosomes) 1
Created with Highcharts 5.0.5: 28.9 %Unique AlignmentsUnique Alignments: 28.9 %: 60.6 %No AlignmentNo Alignment: 60.6 %: 10.5 %Multiple AlignmentsMultiple Alignments: 10.5 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 200,758,561
Methylated C's in CpG context 16,775,795
Methylated C's in CHG context 1,545,637
Methylated C's in CHH context 15,604,110
Methylated C's in Unknown context 1,572,866
Unmethylated C's in CpG context 7,091,116
Unmethylated C's in CHG context 37,129,696
Unmethylated C's in CHH context 122,612,207
Unmethylated C's in Unknown context 3,387,885
Percentage methylation (CpG context) 70.3%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 11.3%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 2,086,448 original top strand
CTOT 2,087,306 complementary to original top strand
CTOB 2,052,271 complementary to original bottom strand
OB 2,047,803 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB0500k1 000k1 500k2 000k2 500k

Deduplication

Alignments analysed 8,273,828
Unique alignments 7,278,446
Duplicates removed 995,382
Duplicated alignments were found at 326089 different positions
Created with Highcharts 5.0.5: 88.0 %UniqueUnique: 88.0 %: 12.0 %DuplicateDuplicate: 12.0 %

Cytosine Methylation after Extraction

Total C's analysed 122,983,889
Methylated C's in CpG context 10,626,266
Methylated C's in CHG context 804,585
Methylated C's in CHH context 6,097,998
Unmethylated C's in CpG context 4,577,584
Unmethylated C's in CHG context 24,001,874
Unmethylated C's in CHH context 76,875,582
Percentage methylation (CpG context) 69.9%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 7.3%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000100k200k300k400k500k600k700k800k900k1 000k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000100k200k300k400k500k600k700k