Bismark

Bismark Processing Report

/Volumes/Serine/wd/18-04-27/zr2096_2_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_2_s1_R2_val_2.fq.gz

Data processed at 18:08 on 2018-04-29


Alignment

Sequence pairs analysed in total 30,325,606
Paired-end alignments with a unique best hit 15,247,793
Pairs without alignments under any condition 8,686,774
Pairs that did not map uniquely 6,391,039
Genomic sequence context not extractable (edges of chromosomes) 3
Created with Highcharts 5.0.5: 50.3 %Unique AlignmentsUnique Alignments: 50.3 %: 28.6 %No AlignmentNo Alignment: 28.6 %: 21.1 %Multiple AlignmentsMultiple Alignments: 21.1 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 375,896,419
Methylated C's in CpG context 35,550,304
Methylated C's in CHG context 2,282,737
Methylated C's in CHH context 16,987,048
Methylated C's in Unknown context 1,561,533
Unmethylated C's in CpG context 13,991,251
Unmethylated C's in CHG context 79,870,209
Unmethylated C's in CHH context 227,214,870
Unmethylated C's in Unknown context 5,108,826
Percentage methylation (CpG context) 71.8%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 7.0%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 3,850,832 original top strand
CTOT 3,774,008 complementary to original top strand
CTOB 3,773,813 complementary to original bottom strand
OB 3,849,137 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB01M2M3M4M5M

Deduplication

Alignments analysed 15,247,790
Unique alignments 14,119,992
Duplicates removed 1,127,798
Duplicated alignments were found at 674697 different positions
Created with Highcharts 5.0.5: 92.6 %UniqueUnique: 92.6 %: 7.4 %DuplicateDuplicate: 7.4 %

Cytosine Methylation after Extraction

Total C's analysed 217,855,622
Methylated C's in CpG context 20,356,245
Methylated C's in CHG context 1,293,967
Methylated C's in CHH context 8,161,912
Unmethylated C's in CpG context 8,346,961
Unmethylated C's in CHG context 46,525,869
Unmethylated C's in CHH context 133,170,668
Percentage methylation (CpG context) 70.9%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 5.8%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000250k500k750k1 000k1 250k1 500k1 750k2 000k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000200k400k600k800k1 000k1 200k1 400k