Bismark

Bismark Processing Report

/Volumes/Serine/wd/18-04-27/zr2096_3_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_3_s1_R2_val_2.fq.gz

Data processed at 18:08 on 2018-04-29


Alignment

Sequence pairs analysed in total 29,548,753
Paired-end alignments with a unique best hit 15,542,349
Pairs without alignments under any condition 7,362,526
Pairs that did not map uniquely 6,643,878
Genomic sequence context not extractable (edges of chromosomes) 2
Created with Highcharts 5.0.5: 52.6 %Unique AlignmentsUnique Alignments: 52.6 %: 24.9 %No AlignmentNo Alignment: 24.9 %: 22.5 %Multiple AlignmentsMultiple Alignments: 22.5 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 424,899,469
Methylated C's in CpG context 47,524,867
Methylated C's in CHG context 2,298,043
Methylated C's in CHH context 10,743,678
Methylated C's in Unknown context 1,088,927
Unmethylated C's in CpG context 13,919,758
Unmethylated C's in CHG context 92,489,635
Unmethylated C's in CHH context 257,923,488
Unmethylated C's in Unknown context 5,034,580
Percentage methylation (CpG context) 77.3%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 4.0%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 3,906,974 original top strand
CTOT 3,851,211 complementary to original top strand
CTOB 3,865,023 complementary to original bottom strand
OB 3,919,139 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB01M2M3M4M5M

Deduplication

Alignments analysed 15,542,347
Unique alignments 14,807,502
Duplicates removed 734,845
Duplicated alignments were found at 572664 different positions
Created with Highcharts 5.0.5: 95.3 %UniqueUnique: 95.3 %: 4.7 %DuplicateDuplicate: 4.7 %

Cytosine Methylation after Extraction

Total C's analysed 267,693,368
Methylated C's in CpG context 29,337,022
Methylated C's in CHG context 1,506,507
Methylated C's in CHH context 6,531,799
Unmethylated C's in CpG context 8,890,935
Unmethylated C's in CHG context 57,632,709
Unmethylated C's in CHH context 163,794,396
Percentage methylation (CpG context) 76.7%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 3.8%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000250k500k750k1 000k1 250k1 500k1 750k2 000k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000200k400k600k800k1 000k1 200k1 400k1 600k