Bismark

Bismark Processing Report

/Volumes/Serine/wd/18-04-27/zr2096_4_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_4_s1_R2_val_2.fq.gz

Data processed at 18:08 on 2018-04-29


Alignment

Sequence pairs analysed in total 23,970,516
Paired-end alignments with a unique best hit 12,995,901
Pairs without alignments under any condition 5,579,694
Pairs that did not map uniquely 5,394,921
Genomic sequence context not extractable (edges of chromosomes) 2
Created with Highcharts 5.0.5: 54.2 %Unique AlignmentsUnique Alignments: 54.2 %: 23.3 %No AlignmentNo Alignment: 23.3 %: 22.5 %Multiple AlignmentsMultiple Alignments: 22.5 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 327,201,316
Methylated C's in CpG context 28,026,421
Methylated C's in CHG context 1,662,436
Methylated C's in CHH context 9,876,129
Methylated C's in Unknown context 887,156
Unmethylated C's in CpG context 13,940,071
Unmethylated C's in CHG context 69,726,438
Unmethylated C's in CHH context 203,969,821
Unmethylated C's in Unknown context 4,128,531
Percentage methylation (CpG context) 66.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 4.6%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 3,325,378 original top strand
CTOT 3,162,868 complementary to original top strand
CTOB 3,171,036 complementary to original bottom strand
OB 3,336,617 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB01M2M3M4M

Deduplication

Alignments analysed 12,995,899
Unique alignments 12,663,182
Duplicates removed 332,717
Duplicated alignments were found at 284466 different positions
Created with Highcharts 5.0.5: 97.4 %UniqueUnique: 97.4 %: 2.6 %DuplicateDuplicate: 2.6 %

Cytosine Methylation after Extraction

Total C's analysed 202,414,211
Methylated C's in CpG context 16,972,342
Methylated C's in CHG context 1,072,413
Methylated C's in CHH context 5,847,499
Unmethylated C's in CpG context 8,759,183
Unmethylated C's in CHG context 42,789,465
Unmethylated C's in CHH context 126,973,309
Percentage methylation (CpG context) 66.0%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 4.4%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000250k500k750k1 000k1 250k1 500k1 750k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000200k400k600k800k1 000k1 200k