Bismark

Bismark Processing Report

/Volumes/Serine/wd/18-04-27/zr2096_8_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_8_s1_R2_val_2.fq.gz

Data processed at 18:08 on 2018-04-29


Alignment

Sequence pairs analysed in total 29,483,218
Paired-end alignments with a unique best hit 14,200,416
Pairs without alignments under any condition 9,943,053
Pairs that did not map uniquely 5,339,749
Genomic sequence context not extractable (edges of chromosomes) 11
Created with Highcharts 5.0.5: 48.2 %Unique AlignmentsUnique Alignments: 48.2 %: 33.7 %No AlignmentNo Alignment: 33.7 %: 18.1 %Multiple AlignmentsMultiple Alignments: 18.1 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 362,392,795
Methylated C's in CpG context 29,294,128
Methylated C's in CHG context 2,164,397
Methylated C's in CHH context 13,260,103
Methylated C's in Unknown context 1,218,115
Unmethylated C's in CpG context 14,337,861
Unmethylated C's in CHG context 75,732,890
Unmethylated C's in CHH context 227,603,416
Unmethylated C's in Unknown context 4,698,419
Percentage methylation (CpG context) 67.1%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 5.5%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 3,629,946 original top strand
CTOT 3,487,296 complementary to original top strand
CTOB 3,468,647 complementary to original bottom strand
OB 3,614,516 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB01M2M3M4M

Deduplication

Alignments analysed 14,200,405
Unique alignments 13,452,841
Duplicates removed 747,564
Duplicated alignments were found at 527675 different positions
Created with Highcharts 5.0.5: 94.7 %UniqueUnique: 94.7 %: 5.3 %DuplicateDuplicate: 5.3 %

Cytosine Methylation after Extraction

Total C's analysed 228,933,398
Methylated C's in CpG context 18,154,221
Methylated C's in CHG context 1,361,580
Methylated C's in CHH context 7,326,787
Unmethylated C's in CpG context 9,182,606
Unmethylated C's in CHG context 47,670,865
Unmethylated C's in CHH context 145,237,339
Percentage methylation (CpG context) 66.4%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 4.8%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000250k500k750k1 000k1 250k1 500k1 750k2 000k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000200k400k600k800k1 000k1 200k1 400k