Bismark

Bismark Processing Report

/Volumes/Serine/wd/18-04-27/zr2096_9_s1_R1_val_1.fq.gz and /Volumes/Serine/wd/18-04-27/zr2096_9_s1_R2_val_2.fq.gz

Data processed at 18:08 on 2018-04-29


Alignment

Sequence pairs analysed in total 31,847,541
Paired-end alignments with a unique best hit 16,108,088
Pairs without alignments under any condition 9,175,758
Pairs that did not map uniquely 6,563,695
Genomic sequence context not extractable (edges of chromosomes) 5
Created with Highcharts 5.0.5: 50.6 %Unique AlignmentsUnique Alignments: 50.6 %: 28.8 %No AlignmentNo Alignment: 28.8 %: 20.6 %Multiple AlignmentsMultiple Alignments: 20.6 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 446,271,830
Methylated C's in CpG context 48,190,808
Methylated C's in CHG context 2,774,831
Methylated C's in CHH context 12,251,657
Methylated C's in Unknown context 1,258,835
Unmethylated C's in CpG context 14,405,999
Unmethylated C's in CHG context 95,964,094
Unmethylated C's in CHH context 272,684,441
Unmethylated C's in Unknown context 5,548,505
Percentage methylation (CpG context) 77.0%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 4.3%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 4,345,578 original top strand
CTOT 3,702,815 complementary to original top strand
CTOB 3,705,364 complementary to original bottom strand
OB 4,354,326 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB01M2M3M4M5M

Deduplication

Alignments analysed 16,108,083
Unique alignments 14,083,414
Duplicates removed 2,024,669
Duplicated alignments were found at 1660011 different positions
Created with Highcharts 5.0.5: 87.4 %UniqueUnique: 87.4 %: 12.6 %DuplicateDuplicate: 12.6 %

Cytosine Methylation after Extraction

Total C's analysed 255,572,824
Methylated C's in CpG context 26,756,008
Methylated C's in CHG context 1,647,018
Methylated C's in CHH context 7,002,283
Unmethylated C's in CpG context 8,438,242
Unmethylated C's in CHG context 54,139,076
Unmethylated C's in CHH context 157,590,197
Percentage methylation (CpG context) 76.0%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 4.3%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000250k500k750k1 000k1 250k1 500k1 750k2 000k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455055606570758001020304050607080901000200k400k600k800k1 000k1 200k1 400k1 600k