Processing paired-end Bismark output file(s) (SAM format): zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_10_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam: 7840800 Total number duplicated alignments removed: 292715 (3.73%) Duplicated alignments were found at: 246728 different position(s) Total count of deduplicated leftover sequences: 7548085 (96.27% of total) Checking file >>zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_1_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam: 5862943 Total number duplicated alignments removed: 408029 (6.96%) Duplicated alignments were found at: 178632 different position(s) Total count of deduplicated leftover sequences: 5454914 (93.04% of total) Checking file >>zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_2_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam: 12405477 Total number duplicated alignments removed: 781960 (6.30%) Duplicated alignments were found at: 549300 different position(s) Total count of deduplicated leftover sequences: 11623517 (93.70% of total) Checking file >>zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_3_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam: 13093746 Total number duplicated alignments removed: 660853 (5.05%) Duplicated alignments were found at: 513074 different position(s) Total count of deduplicated leftover sequences: 12432893 (94.95% of total) Checking file >>zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_4_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam: 10958111 Total number duplicated alignments removed: 302583 (2.76%) Duplicated alignments were found at: 257083 different position(s) Total count of deduplicated leftover sequences: 10655528 (97.24% of total) Checking file >>zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_5_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam: 13585388 Total number duplicated alignments removed: 724362 (5.33%) Duplicated alignments were found at: 605546 different position(s) Total count of deduplicated leftover sequences: 12861026 (94.67% of total) Checking file >>zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_6_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam: 10919623 Total number duplicated alignments removed: 332524 (3.05%) Duplicated alignments were found at: 278765 different position(s) Total count of deduplicated leftover sequences: 10587099 (96.95% of total) Checking file >>zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_7_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam: 12618338 Total number duplicated alignments removed: 447800 (3.55%) Duplicated alignments were found at: 388990 different position(s) Total count of deduplicated leftover sequences: 12170538 (96.45% of total) Checking file >>zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_8_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam: 11642729 Total number duplicated alignments removed: 547980 (4.71%) Duplicated alignments were found at: 437695 different position(s) Total count of deduplicated leftover sequences: 11094749 (95.29% of total) Checking file >>zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_9_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam: 13327617 Total number duplicated alignments removed: 1741377 (13.07%) Duplicated alignments were found at: 1421061 different position(s) Total count of deduplicated leftover sequences: 11586240 (86.93% of total) samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1