05 October 2011
Summary:
-Reviewed basic molecular terms and functions.
-Began RNA isolation procedures on C. gigas gill tissue to protocol, stopped procedure after homogenization step.
-Ran protein isolation procedure on C. gigas digestive gland tissue using CelLytic MT and Bradford Assay.
-Learned how to operate the spectrophotometer.

Methods and Materials:
-Will finish at a later date.

Recordings and Measurments:
1. RNA Isolation procedure used 0.02g C. gigas gill tissue.
-Added a total of 1000ul of Tri-Reagent
2. Protein Extraction procedure used 0.053g of digestive gland tissue.

Sample	Reading
First absorbance	0.261
Second absorbance	0.262
Average	0.2615

Results:
-For protein extraction: Concentration - 530.27ug/ml
Calculation - y=mx+b
m=1013.9
x=0.2615 * 2 (to account for the dilution)
b=0

y=1013.9(2*0.2615)
y=530.27ug/ml

Conclusion:
-RNA isolation went as expected and there is not much detail to give until the procedure is completed.
-Protein extraction results showed a high concentration of protein within the digestive gland. Something to compare
would be this concentration to the other tissue samples as I expect 530.27ug/ml to be a high concentration level.

Next Step:
-Complete RNA isolation procedures.
-Compare concentration levels from other groups in lab.
-Begin putting group project ideas together.

Reflection:
Purpose:
-To understand and perform basic fundamental techniques ( RNA isolation and Protein Extraction) used in molecular biology.

-The RNA isolation procedures in lab are used to measure the amount of mRNA being produced by the organism or the change in expression.
-The protein extraction procedure measures the concentration of protein being secreted by the organism.