SEQUENCE_ID=PGEN_.00g132040 SEQUENCE_TEMPLATE=ATGTCAAGATTTTTCAAGGGATCAGATTCAGAATCTGATAGTTCTTCTGGTGATGAGGAGATAATCCAACAGAGGCCAGCTGTAGCTGCCAAGTAAGTCTAACCTTATCTTACTTAACATTACATGTNNNNNNNNNNAAGTAAGTCTAACCTTATCTTACTTAACATTACATGTATGTAGCTGCAAAGGAAGTCGAATTCATTGTGGTTGTATTTGACTCCACTACTGGAGCATAAAACTTTCTAATAGAACAACTTTAAAAGTTCTCCTAAAACTACAGTTACTTTTGCTTACATATGCAAAACTATTGCCTTTACATATTTTCAAACTAAATATTACAATATAAATGTGTTGTTGCATCATTTTAGAAGTTGTCCTTCTTTATAGTATTTAAAATGTATTAATACTCATTATAAGTATATGGCACTTACCTGATATTGTTTTGCAGGGTTTTCCAGTTCAGTGATGATGAAGAGGAGACAAAAAGAGTTGTCCGTAGTGCTAAAGACAAAAGGTTGGAATTATCATTCAGTTATATTTAATCAAGAAAGTGGTTAATACAAGTAACATTTATTTAAAAAAAAAAACACAATTTAATTTTGTTACTTTTAGGCAATTGCATATAATTATGATATCAGATTATAAAACCTATACTACGGAATTTCAATTGTCGGAAATATATTTTTTATATTGGAAAAAGAATCCTTACTTACCTACCTACATATCTACCTACTTGTCTTCTCAGATTGAAAATGAGGATCAGGAGAGAGCAAGCAGATATTTTAATGTTGGTGTTGCCTACTTGCAGTCACATATTCCCTGTCTGGAACTAGAACGCTTGCATCTGTAATTTGTTTTCTAGGTTTGAGGACTTGCTGACAATCATTAAAAGTTGTATTCATTTGTTTACTAGGTTTGAGGAGTTGCTAACAGTCATAAAAAGTTGTATTAATTTGTTTTCTAGGTTTGAGGAGTTGCTGACAATCATTAAAAGTTTGAAGAACAAGAAGAAGATTAAGGATCATAGTAATGTTCTCTCAGGTGTGTCAATGTTTATTTTGTTACCCTTGTGATTCGTGTGATTCATTTATGTAAATAAATGCAGTTGATTTAACACCTGCTGTTATTAAAGACTACTTTATAAGCATTGTGGACAGGATGATGATATTAATGTTACTCTGAAGCTTAGCACTTTATGAACCACCTCAGGATCTCACACCATATATTTTACATTCGTAATAAATATTATTGCCGTTGATTGTGTTATATTGCATCTTGTGTGTTTGCAAATGTTTAGATAAAACAACAAAAAATGTTCAAATTTAAAAAAAAACATCAAATTAATATGCATAATGAACGTAGAAGTTTCAGCATATTTAATCCAATTCTCACTTTTGTCGCCTGGTATGATATACATGGGCTGTGGTGCCATCACTCGTGCAGCACCCTGGAACCATGGGGGACCTTAGAGTTTGATGCATCTTATAAAGTAGCATGCACACATACAGTTGCTGTTCATGTGTTTCATTTCTTTTTATACCTATAAGTTGATAATCATCATAGTTGGTCTTTCATTTCATGTTGTACAGTCATTTTACAGAGAATCTTTCTTTGATGTTTCAGACTTTGAATCACTGATGAAGGCGTATGACAAAACCAAGAAAGTTGTTGAGAGGGAAGGTATCCCTAGGTTCTACATCAGAACTCTGGCAGAGTTAGAGGACTTTGTTACAGAGGTTTGTTTATTATATATTATAACAGGGTTGGTTGTCTGAGTGGTATACATTTGCAGTAAGAAATGAAATTTGTGACTTTTGATTCTATAACAGTCTGGTTTGTTTTAGGTCTTCCGTGACTATACGATCAAAGTCTATCTCGAGACCACATATCTCTCTGCATTGAGGACAAGGGTTTGAATTGAAATAGGTTTAAAAAAAATTGTGTTAGAAAGTTCACCAATTACTTGCAGAAGGGCAGTGGTTCTTTTCGGATTCAATTTGAGCTTTAAAAATAAGTGCCATATTTAACTGGTATTAAATGGCGTTAAATCCCATTATTATTTTTTTTATAAATGGACCATTTTATAGGCAATAAAAATAACTGCCCAGTGGATAAAATTGAAACTCTGCATACAAAATTGGAAGTAGAGCCAACGCCATGAGTCTTAGTTCTGTATCCTTGTTTTGTACAATTCTACTTTTCATAGTTTTGCTGGGATGCTTTGGCCCTTTGTATCATTATCATTGTTTATCTTTTGGTACTTTAGATTTGAGTCAACTTCTCTATGTTGCATTGCAGTGTTGGGAGGACAGAGAGGGACGCAAGAAGATGAATAAAATCCATGCTAAGAGTCTCACAATTCTCCGTCAGAAACTGAAGAAGTACATTCGTGACTTCGAGCAGGAATTGGAGAAATACCGTGAAAATCCTGATGAGGGTGAAGTGGATGATGAAGCTGATGGAGATGATGAAGGTACGGTAGTAGAGGCAGATGTAGTATAGGCAGATAGTAATGGTGAATTAGTAGAGGCAGATGATGAAGGTAATGTAGTAGAGGCAGATGTAGTAGAGACAGATATAGTAGTTGTTGTATGGTGTTTAGCGTCGCTTTCAACAATTTTTCAGTCAAATCGCGACAGTGTCTACTTGAGACAGTATTGTTAGCGCCGTCACACTGGAGTATCATCCAGCAGACACGTGGTATGATACCCCACCTAGTCACATTATACTGACACCGGGCTGACCAGACCTAGAACTGTCCTCTAAATGCTGAGCACCAAGCGAGGAAGCTTCAGGTACCATTTTTTTGAAGTCTTTGGTATAACTTGGCTGGGGATCAAACCTACAACCTTCCACATGTGAGGCGGACGCTCTACAACTAGACCACGCTGCAGTCAGATGTAGAAGAGGCAGATAGTGAAGGTTAAGTAGTAGAGGCAGATGATGACAGTAATGTAGTAGAGGCAGATAATGAAGGTAATGTAGTAGAGGCAGATGATGACAGTAATGTAGTAGAGGCAGATAATGAAGGTAATGTAGTAGAGGTAGGTGATCGTTGAGGAACCATTGTGAGATATCTGGTGGTCTTTGTGTGATAAATATCTGATGGTCTTTGTGTGATATATCTCTGATGATCATTGTTTGGTATTATCATGGATGACCATTTTGTTATATATCTTTTATTAATGCGTTATGTATCAGAATATCAGTGTGTGATATTGTATCAGATATTGGTGTGTGATATTGTATCAGCTTATCAATGTGTGATATTATATCAGATTAATAACAGTGTGTGATATTGTATCAGATTAACAGTGTGTGATATTGTATCAGATTATCTGTGTTTGATAATGTATCAGATTAACAGTGTATGGTAATGTATCAGATGATCAGTATGTGATATTGTATCAGAGTATCAGTGTGATATTTTTTCAGATGATCAATTTGATATTGTATCAGATTATCAGTGTGTGATATTGTATCAGATTATCAGTGTGGTATTGCATCAGATGATTAGTTTGTGATATTGTATCAGATTATCAGTGTGTGGTATTGAATAAGATTATCAATGATATTGTGCCAGTGTGATATTGTATCAGATTATCAGTGATATTGTATAAGATTATCAGTGTGATATTGTTTCAGATGTTGGTGTGTTATATTGTATCAGATTATCAGTGTGATATTGTATCAGATGTAGGTGTGTAATATTGTATCAGATTATCATTGTGATATTGTATCAGATTATCACTGTGTGATATTGTATCAGATTAACAGTGTGTAGTATTGTATAAGATTATCAATGATATTGTGTCAGTGTGATATTGCATCTGATTACCAGTGATATTGTATCGGATTATCAGTGTGTGCTATTGTATCAGTGTGATATTGTATCATATTATCAGTGAGATATTGTATCATATTATCAGTAATATTGTATCATATATTGGTGTGTGTTATTGTATAAGATTATCAGTGATATTGTATCAGTGTGATAGTGTATCAGATTATCAGTGTGTGATATTGTATCAGATGTCGGTGTATAAAATTGTATAAGATTATCAGTGTGATATGGTATCAGATTATCAGTGTGATATTGTATTACATTATCAGTTATATTTTATCAGTGTGATAGTGTATCAGATTGTCAGTGTGTGATATTGTATCATGTCAGTGTGTGATATGGTATCAGATTATCAGTGTGTGATATATTTTATAAGATTATTAGTGATATTGTATCAGTTTGATAGTGTATCAGATTGTCAGTGTATGATAGTGATTCAGATTATCAGTTTATGATATTGTATAGATATCAGTGTGATATTGTATCAGATTATCATTGTGTGATATTGTATCAGATTATCAGTGTGTGATATTGTATCAGATATCGATGTGTGGTATTGTATCAGATTACCAGTGTGTGATATTGTATCAGATTATCAGTGTGTGATATTGTATCAGATATCGATGTGTGATATTGTATCAGATTANNNNNNNNNNTGTGTGATATTGTATCAGATATCGATGTGTGATATTGTATCAGATTACCAGTGAGTTATTGTCCTGGATGATCAACAAGTGTTACAATCTCAGATGATGATTTTGTGATAAAACTTTTATATGGTTTAATACCAGATGCTTCTGTCGTACAGTTTCAGATGGACATTGCTAATAAAAATCACAAATGATCATTGTGTAATATAATCCCAGATGGTTAGTGTATTATCTCTTAATTAAGACTATTGTGTGACAATATTTCAGATGAAGACAGCTCTGATGATGAGGAAGAAGAACAACCTATGGCCACTGCCTTCCTTGAAAAATCTGCCAAACCCGCTGTAAGTGCTCACGTACCATCTGATAAAACTGAAGATAATTATGCCAAATAA PRIMER_TASK=generic PRIMER_PICK_LEFT_PRIMER=3 PRIMER_PICK_RIGHT_PRIMER=3 PRIMER_OPT_SIZE=18 PRIMER_MIN_SIZE=15 PRIMER_MAX_SIZE=21 PRIMER_MAX_NS_ACCEPTED=1 PRIMER_PRODUCT_SIZE_RANGE=75-150 P3_FILE_FLAG=1 PRIMER_EXPLAIN_FLAG=1 PRIMER_LEFT_NUM_RETURNED=5 PRIMER_RIGHT_NUM_RETURNED=5 PRIMER_INTERNAL_NUM_RETURNED=0 PRIMER_PAIR_NUM_RETURNED=5 PRIMER_PAIR_0_PENALTY=0.397217 PRIMER_LEFT_0_PENALTY=0.048597 PRIMER_RIGHT_0_PENALTY=0.348620 PRIMER_LEFT_0_SEQUENCE=TTAGCGCCGTCACACTGG PRIMER_RIGHT_0_SEQUENCE=TCGCTTGGTGCTCAGCAT PRIMER_LEFT_0=2660,18 PRIMER_RIGHT_0=2783,18 PRIMER_LEFT_0_TM=60.049 PRIMER_RIGHT_0_TM=59.651 PRIMER_LEFT_0_GC_PERCENT=61.111 PRIMER_RIGHT_0_GC_PERCENT=55.556 PRIMER_LEFT_0_SELF_ANY_TH=2.29 PRIMER_RIGHT_0_SELF_ANY_TH=0.54 PRIMER_LEFT_0_SELF_END_TH=0.00 PRIMER_RIGHT_0_SELF_END_TH=0.00 PRIMER_LEFT_0_HAIRPIN_TH=0.00 PRIMER_RIGHT_0_HAIRPIN_TH=41.91 PRIMER_LEFT_0_END_STABILITY=4.0000 PRIMER_RIGHT_0_END_STABILITY=3.7900 PRIMER_PAIR_0_COMPL_ANY_TH=0.00 PRIMER_PAIR_0_COMPL_END_TH=0.00 PRIMER_PAIR_0_PRODUCT_SIZE=124 PRIMER_PAIR_1_PENALTY=0.406478 PRIMER_LEFT_1_PENALTY=0.048597 PRIMER_RIGHT_1_PENALTY=0.357881 PRIMER_LEFT_1_SEQUENCE=TTAGCGCCGTCACACTGG PRIMER_RIGHT_1_SEQUENCE=CTCGCTTGGTGCTCAGCA PRIMER_LEFT_1=2660,18 PRIMER_RIGHT_1=2784,18 PRIMER_LEFT_1_TM=60.049 PRIMER_RIGHT_1_TM=60.358 PRIMER_LEFT_1_GC_PERCENT=61.111 PRIMER_RIGHT_1_GC_PERCENT=61.111 PRIMER_LEFT_1_SELF_ANY_TH=2.29 PRIMER_RIGHT_1_SELF_ANY_TH=0.00 PRIMER_LEFT_1_SELF_END_TH=0.00 PRIMER_RIGHT_1_SELF_END_TH=0.00 PRIMER_LEFT_1_HAIRPIN_TH=0.00 PRIMER_RIGHT_1_HAIRPIN_TH=40.11 PRIMER_LEFT_1_END_STABILITY=4.0000 PRIMER_RIGHT_1_END_STABILITY=4.4100 PRIMER_PAIR_1_COMPL_ANY_TH=0.00 PRIMER_PAIR_1_COMPL_END_TH=0.00 PRIMER_PAIR_1_PRODUCT_SIZE=125 PRIMER_PAIR_2_PENALTY=0.407827 PRIMER_LEFT_2_PENALTY=0.048597 PRIMER_RIGHT_2_PENALTY=0.359230 PRIMER_LEFT_2_SEQUENCE=TTAGCGCCGTCACACTGG PRIMER_RIGHT_2_SEQUENCE=GCTTCCTCGCTTGGTGCT PRIMER_LEFT_2=2660,18 PRIMER_RIGHT_2=2789,18 PRIMER_LEFT_2_TM=60.049 PRIMER_RIGHT_2_TM=60.359 PRIMER_LEFT_2_GC_PERCENT=61.111 PRIMER_RIGHT_2_GC_PERCENT=61.111 PRIMER_LEFT_2_SELF_ANY_TH=2.29 PRIMER_RIGHT_2_SELF_ANY_TH=0.00 PRIMER_LEFT_2_SELF_END_TH=0.00 PRIMER_RIGHT_2_SELF_END_TH=0.00 PRIMER_LEFT_2_HAIRPIN_TH=0.00 PRIMER_RIGHT_2_HAIRPIN_TH=40.11 PRIMER_LEFT_2_END_STABILITY=4.0000 PRIMER_RIGHT_2_END_STABILITY=4.4000 PRIMER_PAIR_2_COMPL_ANY_TH=0.00 PRIMER_PAIR_2_COMPL_END_TH=0.00 PRIMER_PAIR_2_PRODUCT_SIZE=130 PRIMER_PAIR_3_PENALTY=0.407827 PRIMER_LEFT_3_PENALTY=0.048597 PRIMER_RIGHT_3_PENALTY=0.359230 PRIMER_LEFT_3_SEQUENCE=TTAGCGCCGTCACACTGG PRIMER_RIGHT_3_SEQUENCE=AGCTTCCTCGCTTGGTGC PRIMER_LEFT_3=2660,18 PRIMER_RIGHT_3=2790,18 PRIMER_LEFT_3_TM=60.049 PRIMER_RIGHT_3_TM=60.359 PRIMER_LEFT_3_GC_PERCENT=61.111 PRIMER_RIGHT_3_GC_PERCENT=61.111 PRIMER_LEFT_3_SELF_ANY_TH=2.29 PRIMER_RIGHT_3_SELF_ANY_TH=0.00 PRIMER_LEFT_3_SELF_END_TH=0.00 PRIMER_RIGHT_3_SELF_END_TH=0.00 PRIMER_LEFT_3_HAIRPIN_TH=0.00 PRIMER_RIGHT_3_HAIRPIN_TH=36.74 PRIMER_LEFT_3_END_STABILITY=4.0000 PRIMER_RIGHT_3_END_STABILITY=5.0100 PRIMER_PAIR_3_COMPL_ANY_TH=0.00 PRIMER_PAIR_3_COMPL_END_TH=0.00 PRIMER_PAIR_3_PRODUCT_SIZE=131 PRIMER_PAIR_4_PENALTY=0.412392 PRIMER_LEFT_4_PENALTY=0.048597 PRIMER_RIGHT_4_PENALTY=0.363795 PRIMER_LEFT_4_SEQUENCE=TTAGCGCCGTCACACTGG PRIMER_RIGHT_4_SEQUENCE=TAGGTCTGGTCAGCCCGG PRIMER_LEFT_4=2660,18 PRIMER_RIGHT_4=2751,18 PRIMER_LEFT_4_TM=60.049 PRIMER_RIGHT_4_TM=60.364 PRIMER_LEFT_4_GC_PERCENT=61.111 PRIMER_RIGHT_4_GC_PERCENT=66.667 PRIMER_LEFT_4_SELF_ANY_TH=2.29 PRIMER_RIGHT_4_SELF_ANY_TH=0.00 PRIMER_LEFT_4_SELF_END_TH=0.00 PRIMER_RIGHT_4_SELF_END_TH=0.00 PRIMER_LEFT_4_HAIRPIN_TH=0.00 PRIMER_RIGHT_4_HAIRPIN_TH=41.48 PRIMER_LEFT_4_END_STABILITY=4.0000 PRIMER_RIGHT_4_END_STABILITY=5.7300 PRIMER_PAIR_4_COMPL_ANY_TH=0.00 PRIMER_PAIR_4_COMPL_END_TH=0.00 PRIMER_PAIR_4_PRODUCT_SIZE=92 =