FastQCFastQC Report
Fri 20 Mar 2020
113_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename113_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31638462
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT26991368.531185871171614No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA26652548.424094698408538No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1593680.5037160150199462No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGC886950.2803391644005957No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA834610.26379600879461207No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCC701780.22181229921985463No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTC681110.21527911186074722No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTC669830.21171383109583516No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTAT664110.2099059050341954No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTT662060.20925795950511122No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTAT652850.2063469456890793No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTG648310.2049119833954002No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATAC646840.20444735904039837No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATAC628290.19858424217966097No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTT605810.19147896632902067No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTC591590.18698443685410499No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACAC554240.17517918538518087No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTC534830.1690442474732179No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA525310.16603525165034888No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACAC523900.16558959155473488No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACACAT474130.14985873839252994No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT472400.14931193558018085No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTG470150.14860077585313725No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACACAT440500.13922927100565127No Hit
GTATCAACGCAGAGTACGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA437060.13814198680074904No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACACATCT435870.13776586232289043No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACACATCT410630.12978823054040994No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACACATCTGA330320.10440456934979962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)