FastQCFastQC Report
Fri 20 Mar 2020
118_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename118_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29253455
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT15040205.1413414244573845No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA14438414.935625552605667No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA891090.30461017339661245No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG856650.2928372050412507No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA646080.22085596385110748No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG594040.20306661213179777No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGC581130.19865345819835642No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG566070.19350534834261457No Hit
ATCCAGAACTAGCTGCTCCAGCCAGTGTCCAAGTACCTACGGCGGGCGTG529060.18085385128013085No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG487840.16676320796979366No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA473440.16184071249019988No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTC460260.15733526176651613No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTAT457090.15625162908107776No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTT456450.15603285150420695No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTC446440.15261103346596153No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATAC432020.14768170118709056No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCC431210.1474048108163634No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTG428260.14639638292297438No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG414430.1416687362227812No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG371160.12687732098652962No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT368790.12606716027217982No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA367820.12573557550723496No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA363910.1243989812485397No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACAC361470.12356489173671965No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT355040.12136686076909549No Hit
GTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGGCGCTGGGGCAGG354220.12108655199872971No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATAC350000.11964398735123767No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT348530.11914148260436246No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTAT345960.11826295389724051No Hit
GGCTAACAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCG345790.1182048411033842No Hit
AACAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAA324210.11082793468327075No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTC318580.10890337568673512No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTT317510.1085376069254042No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACACAT307530.10512604408607462No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACAC305620.10447312975510072No Hit
GTATCAACGCAGAGTACGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA294170.10055906216889594No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTC293450.10031293739491626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)