FastQCFastQC Report
Fri 20 Mar 2020
127_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename127_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27552329
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT16614576.030187139533648No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA16541886.003804614847623No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1295820.470312328224594No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1145270.4156708494588606No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1031860.3745091748868127No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA589680.21402183459699542No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGC534650.1940489313988665No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATAC451140.16373933397790075No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTAT450790.16361230297445997No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTC405370.14712730818509026No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTT403180.14633245704927522No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTAT400260.14527265553485516No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTT399560.1450185935279736No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACAC395550.1435631811742666No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTC394570.14320749436463248No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCC384720.13963247898208533No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTG379650.13779234416081484No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATAC379340.13767983098633876No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTC373500.1355602279574986No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACACAT338160.12273372606722284No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACAC326000.11832030606196667No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTC324020.11760167352821607No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTCTCTTATACACATCT310040.11252769230506793No Hit
GTATCAACGCAGAGTACGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA305370.11083273577344405No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACACAT276420.10032545706027246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)