FastQCFastQC Report
Fri 20 Mar 2020
132_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename132_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27518702
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA720700.2618946198843245No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA549350.19962787489032005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525340.19090289941727628No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT495300.17998668687207703No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG480920.17476114970829656No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG399940.14533388965802238No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC354160.12869793059280193No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG342030.12429001920221383No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT332190.12071426915411926No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG331950.12062705573831208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324500.11791980595596406No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG320300.11639357117933832No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT318170.11561955211404956No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC302760.11001972404076327No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA296210.10763952456769219No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT284790.10348961953220032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)