FastQCFastQC Report
Fri 20 Mar 2020
178_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename178_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29495389
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT625520.21207382618347567No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA622370.21100586264517482No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC610290.206910307234802No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT518040.1756342321845628No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT507810.17216589345541433No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA472660.16024877651215247No Hit
GTCTAATAATATTCAGCAGTCTTGCATACGTGGTGCTTCAGGCAGACGTC454710.15416307952405714No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG373090.1264909576205284No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG370930.12575863976569354No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG358960.12170037832015032No Hit
GTTATACTGCTGTGGCAAAGCAGGAACAACATACCCTTCCTTCACTAGCG357170.12109350380156031No Hit
GCATTATCATCATCATGCATTATCATCAGCAAATAGGGGCAGGCAGGGCA330820.11215990404466272No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG317020.10748120663877327No Hit
GGGTTACAGATAGATTTCTTCTGGGTTACGTCATTCCATCGTTTGCTTGT314310.10656241895979063No Hit
GTGCAGCAGTCATCATGGCTGTGGTTGCAATGGTGGCAGTGACAGAGGCA303950.10305000554493449No Hit
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA298900.10133787352321409No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)