FastQCFastQC Report
Fri 20 Mar 2020
178_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename178_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29495389
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT609890.20677469281724No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC603740.20468962114722405No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA589650.19991260328860216No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT499110.16921627987344057No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT484770.16435450300384238No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA459790.1558853826270947No Hit
GTCTAATAATATTCAGCAGTCTTGCATACGTGGTGCTTCAGGCAGACGTC453690.153817262759274No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG390680.13245460163281791No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG368790.12503310263173678No Hit
GTTATACTGCTGTGGCAAAGCAGGAACAACATACCCTTCCTTCACTAGCG353180.11974074998637922No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG352500.11951020547652381No Hit
GCATTATCATCATCATGCATTATCATCAGCAAATAGGGGCAGGCAGGGCA311960.10576568425661381No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG307360.10420611845465065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305150.10345684879762053No Hit
GGGTTACAGATAGATTTCTTCTGGGTTACGTCATTCCATCGTTTGCTTGT304000.10306695734712974No Hit
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA298750.10128701811662832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)