FastQCFastQC Report
Fri 20 Mar 2020
221_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename221_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25902415
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA735590.2839851033195167No Hit
TCTCTACATCGTCCACCAAGGAGAATGCAGAAAAAACCAGTACTTCTGAC558800.21573278012880265No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA521020.201147267542428No Hit
GTTAAAAGCTCTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCG500710.19330629981798994No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT472710.18249649694825754No Hit
GTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTTAA467530.18049668341735703No Hit
GCCCCAGAGCCTGCTATGCCCTGTATGCCCCGGTGTGCGGCAGTGACGGC409870.15823621079347233No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA406290.15685410028369942No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA360920.13933835899085084No Hit
TCATAGGGCGCAGCCGTCACCACCATCACCATCATCACCAGGATCAGAAC351110.13555106734256245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337310.1302233787853372No Hit
CTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCGCACTCAGTCC332130.12822356525443668No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC328930.12698815921218157No Hit
CTTCTGACGTGCATAAAACGAGCAAGCCACAAGAGGACTGAGTGCGCAGG326450.12603071952943384No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT320180.12361009581539019No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT314580.1214481352414437No Hit
CATAAGGAGAGGAAGAGGCGTCAATCCTTCAGTTGGTGGCTGACCGTCGG312000.1204520891198755No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG300910.11617063505468507No Hit
GAGATATATTTGTTTAGCGAGTGAGAGGAGGAGATGAGGCCCAGCGTTCT300430.1159853241483468No Hit
GTCCTCTTGTGGCTTGCTCGTTTTATGCACGTCAGAAGTACTGGTTTTTT294340.11363419202417997No Hit
GCCCTGTATGCCCCGGTGTGCGGCAGTGACGGCAGTACCTACGGTAACGA294110.11354539721489289No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG288910.11153786239622832No Hit
GTATCAACGCAGAGTACGGGGAACATAAGGAGAGGAAGAGGCGTCAATCC278550.1075382353344273No Hit
GTATGCCCCGGTGTGCGGCAGTGACGGCAGTACCTACGGTAACGATTGCA278460.10750348953948888No Hit
CGTCAGAAGTACTGGTTTTTTCTGCATTCTCCTTGGTGGACGATGTAGAG273190.10546893021364996No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT272470.10519096385414256No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG268900.10381271398825168No Hit
CAAGAGGACTGAGTGCGCAGGTCCAAGGCTCAAACTCATGGTCATGGATT267890.1034227889561649No Hit
GTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCAAT267390.10322975676206253No Hit
GTCTCTACATCGTCCACCAAGGAGAATGCAGAAAAAACCAGTACTTCTGA265420.10246920991729921No Hit
CTCCTTGGTGGACGATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCC264100.10195960492486898No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT260790.10068173179991133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)