FastQCFastQC Report
Fri 20 Mar 2020
221_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename221_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25902415
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA726980.280661088937074No Hit
TCTCTACATCGTCCACCAAGGAGAATGCAGAAAAAACCAGTACTTCTGAC536110.20697297916043733No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA488550.18861175685742043No Hit
GTTAAAAGCTCTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCG479930.18528388183109568No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT440160.1699301011121936No Hit
GTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTTAA428980.1656139012520647No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA384290.1483606837431954No Hit
GCCCCAGAGCCTGCTATGCCCTGTATGCCCCGGTGTGCGGCAGTGACGGC378390.14608290385278747No Hit
TCATAGGGCGCAGCCGTCACCACCATCACCATCATCACCAGGATCAGAAC376610.14539570924178305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372860.14394796778601532No Hit
CTGCTAATCCATGACCATGAGTTTGAGCCTTGGACCTGCGCACTCAGTCC328520.12682987281301764No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC323900.12504625533951177No Hit
CTTCTGACGTGCATAAAACGAGCAAGCCACAAGAGGACTGAGTGCGCAGG322350.12444785553779444No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT320450.12371433320020546No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT309050.11931319917467154No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT307210.11860284070037484No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA306020.11814342407841122No Hit
GTCCTCTTGTGGCTTGCTCGTTTTATGCACGTCAGAAGTACTGGTTTTTT296420.11443720595164582No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG292670.11298946449587809No Hit
CATAAGGAGAGGAAGAGGCGTCAATCCTTCAGTTGGTGGCTGACCGTCGG286140.11046846404090122No Hit
GCCCTGTATGCCCCGGTGTGCGGCAGTGACGGCAGTACCTACGGTAACGA272450.10518324256637845No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG270570.10445744151655358No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG270240.104330040268446No Hit
GAGATATATTTGTTTAGCGAGTGAGAGGAGGAGATGAGGCCCAGCGTTCT267520.10327994513252914No Hit
CAAGAGGACTGAGTGCGCAGGTCCAAGGCTCAAACTCATGGTCATGGATT267240.10317184710383182No Hit
GTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCAAT266290.10280508593503734No Hit
GTATGCCCCGGTGTGCGGCAGTGACGGCAGTACCTACGGTAACGATTGCA264490.10211017003626882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)