FastQCFastQC Report
Fri 20 Mar 2020
222_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename222_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53808137
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA878497716.326484226725782No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT710101913.196924100903177No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7281841.3532971788263177No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT5306470.9861835580741255No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC2097120.3897403100947353No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC1433170.2663481919100823No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA1426750.2651550638149765No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC1419390.2637872409520515No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT1407040.2614920490557032No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT1406020.2613024866480696No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG1397560.2597302337376966No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1353850.25160692703410265No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC1333330.24779337742170854No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC1269880.2360014805939109No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT1207650.2244363152732829No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT1181580.21959132314876467No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC1177660.21886280879785894No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC1153930.21445269513791196No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC1147990.2133487728816926No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG1117110.20760986391333341No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC1114610.20714525016913335No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA1096440.20376843747628728No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1061030.19718764840343755No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC942870.17522814439756576No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT916210.17027350342941627No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT815460.15154956953815368No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT802990.14923207618208376No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT782700.14546127103415604No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT718400.1335114055333304No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC713600.13261934714446627No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA705170.13105266959902365No Hit
CTCACACACACACACACACACACACACACACACACACACACACACACACA700600.13020335567462593No Hit
CACCCACACACACACACACACACACACACACACACACACACACACACACA679030.1261946682896678No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA658240.12233094039290006No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGT638710.11870137782320916No Hit
GTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT624560.11607166403103679No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTCC584080.10854863828494934No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT580830.10794464041748927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)