FastQCFastQC Report
Fri 20 Mar 2020
254_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename254_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16771613
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1018510.607282078354658No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT761010.45374884335811944No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG431360.25719649028391006No Hit
GAGCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCG358850.21396272380003045No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG347980.2074815344236717No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT331790.19782831860000585No Hit
GTGCAGCAGTCATCATGGCTGTGGTTGCAATGGTGGCAGTGACAGAGGCA221380.13199684490692695No Hit
GTCCTGTGCTTCGTGCATGAAGGCTATGTGCTTCCTAATTATTGTATGTA203440.12130019933085745No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA197710.11788371219870145No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG197650.11784793746433334No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA196410.11710859295405873No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA193210.11520060712109205No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC183240.1092560387602552No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA179940.1072884283700083No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA168650.10055681585307269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)