FastQCFastQC Report
Fri 20 Mar 2020
254_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename254_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16771613
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA955870.569933255674335No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT737520.43974303485299837No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG422410.25186009240733137No Hit
GAGCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCG334900.19968264233142038No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG332290.1981264413864069No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT314850.18772791859673843No Hit
GTGCAGCAGTCATCATGGCTGTGGTTGCAATGGTGGCAGTGACAGAGGCA211230.12594495234298572No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG196220.11699530629522635No Hit
GTCCTGTGCTTCGTGCATGAAGGCTATGTGCTTCCTAATTATTGTATGTA194680.11607708811311113No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA194160.11576704041525404No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA185990.11089571408546095No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA182330.10871345528900529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179590.10707974241952757No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC172280.10272118728234428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)