FastQCFastQC Report
Fri 20 Mar 2020
272_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename272_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27818893
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA5815992.0906619109538256No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT5107941.8361406401038318No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2724070.9792158156688694No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2002930.7199891095594638No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT804690.2892602520164983No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT604770.21739542260002942No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC523410.18814911146895744No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT502380.18058950081155278No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC487800.17534845833009963No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT461530.1659052356971933No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC459100.16503172861695106No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT450790.1620445500832833No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT445620.16018610086317955TruSeq Adapter, Index 11 (95% over 23bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT439200.15787831672525576No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC425650.15300752621608632No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT418710.15051281875234934No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC412300.1482086292937681No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT408650.14689657133373352No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT406040.14595836002532522Illumina PCR Primer Index 11 (95% over 22bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT389360.13996243488193438No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT373940.13441943933570613No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC343350.12342331522681366No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC341830.12287692396674448No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC333910.12002993792743658No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT295010.10604663528487636RNA PCR Primer, Index 11 (96% over 25bp)
CACACACACACACACACACACACACACACACACACACACACACACACCTG292430.10511920801449576No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT288110.10356630653850965Illumina PCR Primer Index 11 (95% over 24bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC284590.10230097940992836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)