FastQCFastQC Report
Fri 20 Mar 2020
272_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename272_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27818893
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA5367051.9292823765489158No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4854091.744889704992934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3627491.3039663368344672No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2303110.8278941940644439No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2215100.7962574211705693No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC489610.17599909529110305No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT466480.1676846019717607No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC466140.16756238287411365No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT442080.15891358437591316No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT414080.14884848221674385No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC409920.14735309561023868No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT401260.14424010329958134No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC398740.1433342441052561No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT384330.13815431117262647No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT381420.13710825948394137No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT366740.1318312702090626No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC364110.13088586954196918No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT340750.12248869859774794No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC301210.10827533647726384No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC301050.10821782160778289No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC297810.1070531455007933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)