FastQCFastQC Report
Fri 20 Mar 2020
280_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename280_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61008799
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA969206815.886344525483938No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT771719912.649321288884904No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7522901.233084427706895No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT5325130.8728462266565843No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC2061110.3378381534768452No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1779030.2916021998728413No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA1472570.24137010138488385No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC1348810.22108450290916232No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC1337350.21920608533860828No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG1331490.21824556815157103No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT1296280.2124742694902091No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT1280930.20995823897467644No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC1211610.19859594351300047No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC1208140.1980271730967856No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1200410.19676014274596687No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT1095730.17960196200551334No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC1088080.17834804451731628No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT1085350.17790056808035182No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC1071140.1755713958571779No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG1052620.1725357681602616No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA1042880.17093927713607343No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC1032440.16922804856394566No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC993940.1629174834272676No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC900750.14764263758085125No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA851630.13959133993114667No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC848790.13912583330807743No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT818270.1341232762179108No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT792530.1299042126693889No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA771930.1265276505443092No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT717760.11764860344161175No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT713900.11701590782011624No Hit
CACCCACACACACACACACACACACACACACACACACACACACACACACA642300.10527989577372274No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGT640230.10494060045338706No Hit
CTCACACACACACACACACACACACACACACACACACACACACACACACA637250.1044521463207299No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT633420.10382436802271751No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)