FastQCFastQC Report
Fri 20 Mar 2020
280_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename280_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61008799
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA847398613.889776784493005No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT777550712.744894388102937No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6410011.050669756669034No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6324821.0367061970847846No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC1851450.3034726187611069No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1654950.2712641499466331No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA1349710.2212320226136561No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1202360.19707976877237002No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC1171950.1920952418683082No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT1164660.19090033226190864No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT1158800.18993981507487143No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC1151910.1888104697815802No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG1123690.18418490749178656No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC1104080.18097061704165002No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC1088160.17836115737993793No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC979880.1606128978215093No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT966450.1584115760088967No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC959670.15730026090171026No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT947330.15527760184231787No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG935650.15336312389955423No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC930470.15251406604480117No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC928890.1522550870080232No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA918070.15048157233844253No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTT821090.13458550462532462No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC803960.13177771291645982No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC792600.12991568642418283No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT779510.1277700942777123No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT752210.12329532990806785No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA748190.12263640856132901No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT686530.11252966969567783No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT673630.11041522059793375No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGT618740.10141815773164131No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT616520.10105427579389No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)