FastQCFastQC Report
Fri 20 Mar 2020
294_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename294_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28539233
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA627210.2197711480192898No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT626670.2195819348053257No Hit
GTCTAATAATATTCAGCAGTCTTGCATACGTGGTGCTTCAGGCAGACGTC580680.20346727608271742No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC565390.19810973896880832No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT532420.18655722107177863No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA505090.17698093007615165No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA485470.17010618330212307No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT468700.1642300618240161No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT466470.16344868132931253No Hit
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA335390.11751892561373321No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG326430.11437938784129202No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT326060.11424974175024255No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG317440.11122933822363061No Hit
GTTATACTGCTGTGGCAAAGCAGGAACAACATACCCTTCCTTCACTAGCG312480.10949137981388637No Hit
GTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTTAA309850.10856984138291313No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG306230.10730141205967238No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT291160.10202096181071159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)