FastQCFastQC Report
Fri 20 Mar 2020
334_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename334_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25916895
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA1071810.4135564850650512No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA798160.30796899088413177No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG606730.23410597604381234No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG604860.23338443899240244No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG572700.22097554510291453No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT551030.21261420397775274No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG528250.20382457080603214No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT516880.1994374711939837No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG478740.18472120213474646No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC464220.17911867914732843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449460.1734235524741679No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT439780.16968853714922255No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC425720.1642635045594775No Hit
CAATTATTTATTGCAAATATTTTCACGTGTATGGAAAGTAAATTAACACT421270.1625464778863363No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT381310.14712796421021884No Hit
AACAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAA380540.14683086071846183No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT379370.1463794177504674No Hit
TTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAGCAGA369160.14243990261950748No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA364470.14063027226062383No Hit
GGCTAACAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCG318920.12305486440408853No Hit
GTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGGCGCTGGGGCAGG301930.11649929515090446No Hit
TATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACATC300910.11610572948649905No Hit
ATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAGT293260.11315398700345856No Hit
CAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGATGCAAGAT287620.11097780038851104No Hit
ATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGT285040.10998231076677974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284560.10979710339529485No Hit
CTCTAAGCAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGAT282840.10913344364747399No Hit
GTCTAATAATATTCAGCAGTCTTGCATACGTGGTGCTTCAGGCAGACGTC279130.10770194500537197No Hit
GCTCCAGCCAGTGTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGG272760.10524408884629119No Hit
ATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGC271710.10483894772116799No Hit
ATCCAGAACTAGCTGCTCCAGCCAGTGTCCAAGTACCTACGGCGGGCGTG270790.10448396692582194No Hit
ATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATGTACTAAGATTAT267510.10321838322067516No Hit
GTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAG266550.10284796847770537No Hit
GTAAATTAACACTCTAAGCAATTAAGGAAGTCAATAGTCACCTGAATAGC265280.10235794064065158No Hit
ACTTACAAGGGCTAACAAGGACCTGCCATGCTACACGGACAGAATTCTGA265160.10231163879778037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)