FastQCFastQC Report
Fri 20 Mar 2020
349_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename349_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32868756
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA5652051.7195813556192998No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4880151.4847382724189502No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1192900.36292824711711025No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT990690.30140781719880116No Hit
GCCAAGGACGGATCTGCCAAGGACGGATCTGCCAAGGACGGATCTGCCAA550000.16733216188650402No Hit
GTCCTTGGCAGATCCGTCCTTGGCAGATCCGTCCTTGGCAGATCCGTCCT459540.1398105848605892No Hit
TGGCAGATCCGTCCTTGGCAGATCCGTCCTTGGCAGATCCGTCCTTGGCA447960.13628748225214243No Hit
GATCTGCCAAGGACGGATCTGCCAAGGACGGATCTGCCAAGGACGGATCT355250.10808136456396462No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT332640.10120249150895762No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)