FastQCFastQC Report
Fri 20 Mar 2020
349_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename349_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32868756
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA5189901.5789767035904858No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4711951.4335650549111136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1665510.5067152526247115No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1152410.3506095575993201No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA981950.2987487570262775No Hit
GCCAAGGACGGATCTGCCAAGGACGGATCTGCCAAGGACGGATCTGCCAA538080.16370561757798197No Hit
GTCCTTGGCAGATCCGTCCTTGGCAGATCCGTCCTTGGCAGATCCGTCCT475010.14451718221401502No Hit
TGGCAGATCCGTCCTTGGCAGATCCGTCCTTGGCAGATCCGTCCTTGGCA433480.1318820827901123No Hit
GATCTGCCAAGGACGGATCTGCCAAGGACGGATCTGCCAAGGACGGATCT354840.1079566260432856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)