FastQCFastQC Report
Fri 20 Mar 2020
359_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename359_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27274149
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA567660.20813115012314407No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA438560.16079695098827831No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG385240.1412473034447381No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT342520.12558411996649282No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG307930.11290178109681807No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT306380.11233347738915703No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA305350.11195583040922742No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT289540.10615913259108468No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG288730.10586214807288763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)