FastQCFastQC Report
Fri 20 Mar 2020
425_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename425_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66224932
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1124654216.982338313310763No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1067030116.112211334546934No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA19060232.8781048805002927No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT18216162.7506498609919294No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC2953690.4460087629837053No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT2094740.3163068555510182No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC2079430.3139950373976979No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT2056440.3105235351544038No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC2037160.3076122448868652No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC2016650.30451522396429187No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG1968020.2971720680664497No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1959360.2958644034545781No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA1873690.2829281878311328No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC1836510.277313988030973No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT1787260.2698772118029506No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC1733220.26171714302402No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC1732570.26161899267786337No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT1728480.2610014004997393No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC1694670.2558960724942685No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC1675780.25304367243442394No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1667470.2517888580089444No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG1643100.24810897503073315No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA1569670.2370210059256837No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC1478260.22321804724540906No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT1381410.20859364566806954No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT1237580.18687523907159317No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT1210780.1828284247992886No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1181130.17835125900922028No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT1120330.16917042663026063No Hit
GTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1090000.16459058047805922No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA1033530.15606358040503537No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTT1022950.15446599477067036No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1009990.1525090278688395No Hit
CACCCACACACACACACACACACACACACACACACACACACACACACACA1006010.15190804574929576No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT998540.15078007177115713No Hit
GTGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT859370.1297653276563576No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGT850600.12844105298590566No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTGA845180.12762263009949182No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC843300.1273387490983003No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGA802590.12119151741824363No Hit
CTCACACACACACACACACACACACACACACACACACACACACACACACA800580.12088800634782078No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTGT731070.11039195933036217No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA693410.10470527927457896No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA669020.10102237628571667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)