FastQCFastQC Report
Fri 20 Mar 2020
445_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename445_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30745388
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA23324887.586464675612485No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT15239304.956613330103364No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1427380.46425824907462543No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA957440.3114093079586441No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA889500.28931168473138147No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT823150.2677312122390519No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGC692040.22508741798932574No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA566900.18438537838585742No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTC559630.1820207960946858No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTC550120.17892764924612434No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTG537660.17487500889564314No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTT534300.17378216205955832No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTAT529340.17216891196819503No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATAC481950.15675521805091547No Hit
GTATCAACGCAGAGTACGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA458790.14922238093075946No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA446530.14523479098718806No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACT396490.1289591791783535No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG394690.12837372551616522No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACAC384360.12501387199927352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)