FastQCFastQC Report
Fri 20 Mar 2020
463_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename463_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19531145
Sequences flagged as poor quality0
Sequence length150
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT16219618.304484964911172No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT10942135.602400678506047No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT8785074.497980021140593TruSeq Adapter, Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT6968413.567845100735262No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT5974653.059037245384231RNA PCR Primer, Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT5022372.571467264208012Illumina PCR Primer Index 12 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT2632891.34804692710028TruSeq Adapter, Index 12 (96% over 27bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCT2104041.0772742714264831Illumina PCR Primer Index 12 (96% over 25bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA1356520.6945419738576515No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG1263670.6470025182855382TruSeq Adapter, Index 12 (96% over 25bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTG1218510.6238804739814282Illumina PCR Primer Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC1107160.5668689674875692No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1066820.5462147764506382No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT864960.44286190082557886RNA PCR Primer, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTC856510.43853547756672734No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA726820.37213384059152704No Hit
GCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG708250.36262594947710436No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG688820.3526777359955087TruSeq Adapter, Index 12 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCT670580.34333880578941994No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCTTG547350.2802447065955426Illumina PCR Primer Index 12 (96% over 27bp)
GCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCG522810.26768015904853504No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCGTAGT519000.26572942856140797No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT516440.26441870151493935No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC511450.26186380777983065Illumina PCR Primer Index 5 (96% over 25bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGC438520.22452344703805127Illumina PCR Primer Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCG278770.14273100732189536Illumina PCR Primer Index 12 (95% over 21bp)
GATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT263480.13490248523576062TruSeq Adapter, Index 12 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCG202410.10363447713894909RNA PCR Primer, Index 12 (95% over 23bp)
GATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCT196510.10061366089904099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)