FastQCFastQC Report
Fri 20 Mar 2020
463_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename463_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19531145
Sequences flagged as poor quality0
Sequence length150
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG753848938.59727117892986No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1175190.6017005147419673No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1091890.559050685456485No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA615520.31514793423529447No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT612330.31351464545473395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)