FastQCFastQC Report
Fri 20 Mar 2020
481_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename481_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50592084
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA584438411.551973229645967No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT43767998.651153805010285No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2734820.5405628279712692No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2136810.4223605416214916No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1205320.23824280494157943No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC1160580.22939952424177665No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1156420.2285772612173873No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC738370.1459457570476836No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC736630.14560182972498228No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG735040.14528755130941037No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA727070.14371220604393367No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT703530.1390593042184228No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT683030.13500728691073488No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC665570.13155615412087No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA661300.1307121485645857No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC636260.12576275766778058No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA622890.12312005174564464No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG587320.11608930756835398No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC587290.1160833777869281No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC585160.11566236330569027No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT571390.11294059363120919No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC570390.11274293425034636No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT564170.11151349290137959No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA563610.1114028036480964No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC525480.10386605145579692No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT522670.10331062859557237No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC519260.10263661010683015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)