FastQCFastQC Report
Fri 20 Mar 2020
485_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename485_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26010208
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG700710.26939807632449536No Hit
ATCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGC638280.24539596146251502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608340.23388509618992667No Hit
ATCACCATATTGGGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGC581590.22360067247443774No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT439140.1688337132867219No Hit
TCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCC438130.16844540420438006No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA428060.16457384731410069No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG415730.15983340079402672No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC410990.15801103935808586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403150.1549968381644622No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA396700.152517042539606No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCAAGGAGTCCAGACCCTGCCTG381200.14655784375119182No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT375500.14436639645480728No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA367850.14142524350439645No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCATGGAGTCCAGACCCTGCCTG354980.13647718618782287No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT345390.13279017222776535No Hit
TTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAGCAGA335980.12917236186654102No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG305560.11747695366373079No Hit
TCACCATATTGGGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCC268130.10308644975080553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)