FastQCFastQC Report
Fri 20 Mar 2020
72_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename72_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27249335
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT666000.2444096342167616No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA557280.20451141284732272No Hit
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA540900.1985002569787483No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA515210.18907250397119782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430750.1580772521604656No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG427550.1569029115756403No Hit
TCTCTACATCGTCCACCAAGGAGAATGCAGAAAAAACCAGTACTTCTGAC422130.15491387221009248No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA410040.15047706668804944No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG403130.14794122498769235No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT372030.13652810242892166No Hit
CAATTATTTATTGCAAATATTTTCACGTGTATGGAAAGTAAATTAACACT361710.13274085404286012No Hit
GTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTTAA346820.127276500509095No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT346580.12718842496523308No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT304430.1117201575744876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)