FastQCFastQC Report
Fri 20 Mar 2020
72_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename72_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27249335
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1041000.3820276715009742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT747960.27448743244559914No Hit
ATGTAGAGACCAGGCTTATTGTTGCAAATCCTCTCCGTGGACAGGTTGCA544630.19986909772293526No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA526690.19328545081925852No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT483710.17751258883932397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462610.1697692806081323No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA449410.16492512569572798No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG414900.15226059645125284No Hit
TCTCTACATCGTCCACCAAGGAGAATGCAGAAAAAACCAGTACTTCTGAC411660.15107157660911724No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG393280.14432645787502704No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA391660.14373194795395924No Hit
CAATTATTTATTGCAAATATTTTCACGTGTATGGAAAGTAAATTAACACT338540.12423789424585957No Hit
CTGTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTT321160.11785975694452726No Hit
GTACATCATATTATATTAGAATAACATTCAAGTGCAGCATCAACTGTTAA316770.11624870845472009No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT297080.10902284404371704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291490.10697141783460036No Hit
TCATAGGGCGCAGCCGTCACCACCATCACCATCATCACCAGGATCAGAAC290420.1065787477015494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)