-------------------------------------------------------------------------------- SUMMARY -------------------------------------------------------------------------------- - Sat Dec 09 05:04:39 2017 - [HM2JYBBXX_Geoduck-2_supernova] - commit hash = b5305e091 - assembly checksum = 1,694,464,505,766,402,869 -------------------------------------------------------------------------------- INPUT - 1200.03 M = READS = number of reads; ideal 800M-1200M for human - 139.50 b = MEAN READ LEN = mean read length after trimming; ideal 140 - 54.05 x = EFFECTIVE COV = effective read coverage; ideal ~42 for nominal 56x cov - 68.74 % = READ TWO Q30 = fraction of Q30 bases in read 2; ideal 75-85 - 402.00 b = MEDIAN INSERT = median insert size; ideal 0.35-0.40 - 81.78 % = PROPER PAIRS = fraction of proper read pairs; ideal >= 75 - 21.14 Kb = MOLECULE LEN = weighted mean molecule size; ideal 50-100 - 165.00 b = HETDIST = mean distance between heterozygous SNPs - 5.80 % = UNBAR = fraction of reads that are not barcoded - 950.00 = BARCODE N50 = N50 reads per barcode - 27.56 % = DUPS = fraction of reads that are duplicates - 37.36 % = PHASED = nonduplicate and phased reads; ideal 45-50 -------------------------------------------------------------------------------- OUTPUT - 17.40 K = LONG SCAFFOLDS = number of scaffolds >= 10 kb - 3.34 Kb = EDGE N50 = N50 edge size - 15.12 Kb = CONTIG N50 = N50 contig size - 63.34 Kb = PHASEBLOCK N50 = N50 phase block size - 61.64 Kb = SCAFFOLD N50 = N50 scaffold size - 712.48 Mb = ASSEMBLY SIZE = assembly size (only scaffolds >= 10 kb) --------------------------------------------------------------------------------