17-03-18 00:05:46.678 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:05:46.678 -07 000000610 INF: Y Motor: Entering MoveAbsStepped (target = 120609 steps, sync = True, waitForSettling = True) 17-03-18 00:05:46.678 -07 000000610 INF: Y Motor: Moving to 75.3806 mm, started at 74.8320 mm, delta = 0.5485 mm 17-03-18 00:05:46.678 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:05:46.678 -07 000000431 INF: FPGA: Recv YRDPOS 153256 17-03-18 00:05:46.688 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:05:46.688 -07 000000610 INF: Y Motor: Encoder = 153256 = motor step 119731.3 = 74.8320 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:05:46.688 -07 000000610 INF: Y Motor: Last commanded position = 74.8526 mm (119764.2 steps), delta from last commanded position = 0.5280 mm (844.8 steps). UsingTableMove = False 17-03-18 00:05:46.688 -07 000000610 INF: Y Motor: Moving to 154379 encoder counts 17-03-18 00:05:46.688 -07 000000610 INF: FPGA: Xmit YMVTO 154379\n [Y Motor] 17-03-18 00:05:46.798 -07 000000576 INF: FPGA: Recv YMVTO 154377 872 17-03-18 00:05:46.798 -07 000000610 INF: FPGA: 'YMVTO 154379' command took 0 ms to send, 106 ms to get response, and 115 ms overall 17-03-18 00:05:46.798 -07 000000610 INF: Y Motor: Position error before settling = -2 encoder counts (-0.001 mm) 17-03-18 00:05:46.798 -07 000000610 INF: Y Motor: To move from 74.832 to 75.381 (0.549 mm) should take 877 steps, but required 872 steps (0.545 mm). Slippage = -0.003 mm (-0.01%). 17-03-18 00:05:46.798 -07 000000610 INF: Y Motor: FPGA move command 'YMVTO 154379' took 115 ms, distance = 0.549 mm, average velocity = 4.770 mm/sec 17-03-18 00:05:47.128 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:05:47.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1296, R2=1347, Total=2643. Warn if over 250 bubbles per interval. 17-03-18 00:05:47.388 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:05:49.798 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:05:49.798 -07 000000482 INF: FPGA: Recv YRDPOS 154380 17-03-18 00:05:49.808 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:05:49.808 -07 000000610 INF: Y Motor: Encoder = 154380 = motor step 120609.4 = 75.3809 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:05:49.828 -07 000000610 INF: Y Motor: After move to 75.380580, error = 0.000279, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:05:49.828 -07 000000610 INF: Y Motor: Position error after settling = 1 encoder counts (0.000 mm), Delta during settling = 3 counts (0.001 mm) 17-03-18 00:05:49.828 -07 000000610 INF: Y Motor: Moved to 75.3809 after 3147 ms 17-03-18 00:05:49.828 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 60° =================== 17-03-18 00:05:49.828 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 60°, FC temperature to 60° =================== 17-03-18 00:05:49.828 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 60000000\n [FlowCellTempControl] 17-03-18 00:05:49.838 -07 000000618 INF: FPGA: Recv TEC2TEMP 17-03-18 00:05:49.838 -07 000000610 INF: FPGA: 'TEC2TEMP 0 60000000' command took 1 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:05:49.838 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:05:49.838 -07 000000476 INF: FPGA: Recv TEC2EN 17-03-18 00:05:49.848 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:05:49.848 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 60° =================== 17-03-18 00:05:49.848 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:05:49.848 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:05:49.848 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:05:49.878 -07 000000610 INF: ReagentValve: Recv 18\r 17-03-18 00:05:49.878 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 24 to 3. Moving 3 positions. 17-03-18 00:05:49.878 -07 000000610 INF: ReagentValve: Xmit +03\r 17-03-18 00:05:50.178 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:05:50.198 -07 000000610 INF: ReagentValve: Recv 0278\r 17-03-18 00:05:50.198 -07 000000610 INF: ReagentValve: Move+ from 24 to 3 took 632 steps, expected 612. Error = 20 steps (3.3%) 17-03-18 00:05:50.248 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:05:50.268 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:05:50.268 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:05:50.368 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:05:50.368 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:05:50.368 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:05:50.398 -07 000000610 INF: LanePump: Recv /0`0(0x03)\r\n 17-03-18 00:05:50.398 -07 000000610 INF: LanePump: Current Position of Pump is: 0 17-03-18 00:05:50.398 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:05:50.528 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:05:50.568 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:05:52.128 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:05:52.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1296, R2=1347, Total=2643. Warn if over 250 bubbles per interval. 17-03-18 00:05:52.748 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:05:53.698 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:05:53.728 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:05:54.148 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:05:54.178 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:05:54.598 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:05:54.628 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:05:54.628 -07 000000610 INF: LanePump: Waited 4059 ms for pump motion to complete 17-03-18 00:05:54.628 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:05:54.758 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:05:54.788 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:05:54.788 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:05:54.918 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:05:54.948 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:05:54.948 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:05:54.948 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:05:55.078 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:05:55.108 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:05:55.538 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:05:55.568 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:05:55.568 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:05:55.568 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:05:56.788 -07 000000610 INF: AsyncDelayTimer waited 1213ms 17-03-18 00:05:56.788 -07 000000610 INF: ReagentValve: Go to position 4 - PumpToFlowcell start 17-03-18 00:05:56.788 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:05:56.808 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:05:56.808 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 3 to 4. Moving 1 positions. 17-03-18 00:05:56.808 -07 000000610 INF: ReagentValve: Xmit +04\r 17-03-18 00:05:56.948 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:05:56.968 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:05:56.968 -07 000000610 INF: ReagentValve: Move+ from 3 to 4 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:05:57.018 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:05:57.038 -07 000000610 INF: ReagentValve: Recv 04\r 17-03-18 00:05:57.038 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:05:57.128 -07 000000482 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 5 R1 5 17-03-18 00:05:57.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=5, R2=5, Total=10. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1301, R2=1352, Total=2653. Warn if over 250 bubbles per interval. 17-03-18 00:05:57.138 -07 000000610 INF: =================== Cycle #82, Pumping Reagent CMS (4) =================== 17-03-18 00:05:57.138 -07 000000610 INF: LanePump: Pumping from 4, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 45 µL, calling command Pumping to Flow Cell #1 17-03-18 00:05:57.138 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:05:57.168 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:05:57.168 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:05:57.168 -07 000000610 INF: =================== Cycle #82, Pumping Reagent CMS (4), Aspirating port InputPort =================== 17-03-18 00:05:57.298 -07 000000610 INF: LanePump: Xmit /1V400IP270R\r 17-03-18 00:05:57.338 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:05:58.118 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:05:58.778 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:05:58.808 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:05:59.228 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:05:59.258 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:05:59.258 -07 000000610 INF: LanePump: Waited 1919 ms for pump motion to complete 17-03-18 00:05:59.258 -07 000000610 INF: =================== Cycle #82, Pumping Reagent CMS (4) =================== 17-03-18 00:05:59.388 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:05:59.418 -07 000000610 INF: LanePump: Recv /0`990(0x03)\r\n 17-03-18 00:05:59.418 -07 000000610 INF: LanePump: Current Position of Pump is: 990 17-03-18 00:05:59.548 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:05:59.578 -07 000000610 INF: LanePump: Recv /0`990(0x03)\r\n 17-03-18 00:05:59.578 -07 000000610 INF: LanePump: Current Position of Pump is: 990 17-03-18 00:05:59.578 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:05:59.708 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:05:59.738 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:00.168 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:00.198 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:00.198 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:06:00.198 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:06:01.418 -07 000000610 INF: AsyncDelayTimer waited 1218ms 17-03-18 00:06:01.418 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:06:01.418 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:06:01.438 -07 000000610 INF: ReagentValve: Recv 04\r 17-03-18 00:06:01.438 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 4 to 3. Moving 1 positions. 17-03-18 00:06:01.438 -07 000000610 INF: ReagentValve: Xmit -03\r 17-03-18 00:06:01.588 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:06:01.608 -07 000000610 INF: ReagentValve: Recv 00E1\r 17-03-18 00:06:01.608 -07 000000610 INF: ReagentValve: Move- from 4 to 3 took 225 steps, expected 204. Error = 21 steps (10.3%) 17-03-18 00:06:01.658 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:06:01.678 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:06:01.678 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:06:01.778 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:06:01.778 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 20 µL, calling command Pumping to Flow Cell #1 17-03-18 00:06:01.778 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:01.808 -07 000000610 INF: LanePump: Recv /0`990(0x03)\r\n 17-03-18 00:06:01.808 -07 000000610 INF: LanePump: Current Position of Pump is: 990 17-03-18 00:06:01.808 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:06:01.938 -07 000000610 INF: LanePump: Xmit /1V400IP120R\r 17-03-18 00:06:01.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:02.128 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 1 17-03-18 00:06:02.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=1, R2=1, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1302, R2=1353, Total=2655. Warn if over 250 bubbles per interval. 17-03-18 00:06:02.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:02.878 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:02.878 -07 000000610 INF: LanePump: Waited 899 ms for pump motion to complete 17-03-18 00:06:02.878 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:06:03.008 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:03.038 -07 000000610 INF: LanePump: Recv /0`1110(0x03)\r\n 17-03-18 00:06:03.038 -07 000000610 INF: LanePump: Current Position of Pump is: 1110 17-03-18 00:06:03.168 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:03.198 -07 000000610 INF: LanePump: Recv /0`1110(0x03)\r\n 17-03-18 00:06:03.198 -07 000000610 INF: LanePump: Current Position of Pump is: 1110 17-03-18 00:06:03.198 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:06:03.328 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:06:03.358 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:03.498 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:06:03.788 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:03.818 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:03.818 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:06:03.818 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:06:03.818 -07 000000429 INF: WaitCommand(10000) start 17-03-18 00:06:03.818 -07 000000431 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:06:03.818 -07 000000431 INF: =================== Dispense to DispenseAndWait, Dispensing port OutputPort =================== 17-03-18 00:06:05.038 -07 000000431 INF: AsyncDelayTimer waited 1216ms 17-03-18 00:06:05.038 -07 000000431 INF: LanePump: Xmit /1?\r 17-03-18 00:06:05.068 -07 000000431 INF: LanePump: Recv /0`1110(0x03)\r\n 17-03-18 00:06:05.068 -07 000000431 INF: LanePump: Current Position of Pump is: 1110 17-03-18 00:06:05.198 -07 000000431 INF: LanePump: Xmit /1V1500OA0R\r 17-03-18 00:06:05.238 -07 000000431 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:06.768 -07 000000431 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:06.798 -07 000000431 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:07.128 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:06:07.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1302, R2=1353, Total=2655. Warn if over 250 bubbles per interval. 17-03-18 00:06:07.218 -07 000000431 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:07.248 -07 000000431 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:07.248 -07 000000431 INF: LanePump: Waited 2009 ms for pump motion to complete 17-03-18 00:06:07.248 -07 000000431 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:06:07.248 -07 000000431 INF: =================== =================== 17-03-18 00:06:08.858 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.018051, 17-03-18 00:06:12.128 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:06:12.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1302, R2=1353, Total=2655. Warn if over 250 bubbles per interval. 17-03-18 00:06:13.828 -07 000000429 INF: WaitCommand(10000) end 17-03-18 00:06:13.828 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:06:13.828 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 250 µL, calling command Pumping to Flow Cell #1 17-03-18 00:06:13.828 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:13.868 -07 000000610 INF: LanePump: Recv /0`0(0x03)\r\n 17-03-18 00:06:13.868 -07 000000610 INF: LanePump: Current Position of Pump is: 0 17-03-18 00:06:13.868 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:06:13.998 -07 000000610 INF: LanePump: Xmit /1V400IP1500R\r 17-03-18 00:06:14.038 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:14.228 -07 000000482 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:06:17.128 -07 000000476 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 1 17-03-18 00:06:17.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=1, R2=1, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1303, R2=1354, Total=2657. Warn if over 250 bubbles per interval. 17-03-18 00:06:19.598 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:06:20.168 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:20.198 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:20.618 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:20.648 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:21.068 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:21.098 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:21.518 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:21.548 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:21.968 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:21.998 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:21.998 -07 000000610 INF: LanePump: Waited 7959 ms for pump motion to complete 17-03-18 00:06:21.998 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:06:22.128 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:22.128 -07 000000448 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 1 17-03-18 00:06:22.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=1, R2=1, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1304, R2=1355, Total=2659. Warn if over 250 bubbles per interval. 17-03-18 00:06:22.158 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:06:22.158 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:06:22.288 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:22.318 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:06:22.318 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:06:22.318 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:06:22.448 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:06:22.478 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:22.908 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:22.938 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:22.938 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:06:22.938 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:06:24.158 -07 000000610 INF: AsyncDelayTimer waited 1219ms 17-03-18 00:06:24.158 -07 000000610 INF: ReagentValve: Go to position 1 - PumpToFlowcell start 17-03-18 00:06:24.158 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:06:24.178 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:06:24.178 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 3 to 1. Moving 2 positions. 17-03-18 00:06:24.178 -07 000000610 INF: ReagentValve: Xmit -01\r 17-03-18 00:06:24.388 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:06:24.408 -07 000000610 INF: ReagentValve: Recv 0198\r 17-03-18 00:06:24.408 -07 000000610 INF: ReagentValve: Move- from 3 to 1 took 408 steps, expected 408. Error = 0 steps (0.0%) 17-03-18 00:06:24.458 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:06:24.478 -07 000000610 INF: ReagentValve: Recv 01\r 17-03-18 00:06:24.478 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:06:24.578 -07 000000610 INF: =================== Cycle #82, Pumping Reagent IMT (1) =================== 17-03-18 00:06:24.578 -07 000000610 INF: LanePump: Pumping from 1, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 45 µL, calling command Pumping to Flow Cell #1 17-03-18 00:06:24.578 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:24.608 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:06:24.608 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:06:24.608 -07 000000610 INF: =================== Cycle #82, Pumping Reagent IMT (1), Aspirating port InputPort =================== 17-03-18 00:06:24.738 -07 000000610 INF: LanePump: Xmit /1V400IP270R\r 17-03-18 00:06:24.778 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:24.978 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:06:26.218 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:26.248 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:26.668 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:26.698 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:26.698 -07 000000610 INF: LanePump: Waited 1919 ms for pump motion to complete 17-03-18 00:06:26.698 -07 000000610 INF: =================== Cycle #82, Pumping Reagent IMT (1) =================== 17-03-18 00:06:26.828 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:26.858 -07 000000610 INF: LanePump: Recv /0`1770(0x03)\r\n 17-03-18 00:06:26.858 -07 000000610 INF: LanePump: Current Position of Pump is: 1770 17-03-18 00:06:26.988 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:27.018 -07 000000610 INF: LanePump: Recv /0`1770(0x03)\r\n 17-03-18 00:06:27.018 -07 000000610 INF: LanePump: Current Position of Pump is: 1770 17-03-18 00:06:27.018 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:06:27.128 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 1 17-03-18 00:06:27.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=1, R2=0, Total=1. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1355, Total=2660. Warn if over 250 bubbles per interval. 17-03-18 00:06:27.148 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:06:27.178 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:27.608 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:27.638 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:27.638 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:06:27.638 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:06:28.858 -07 000000610 INF: AsyncDelayTimer waited 1219ms 17-03-18 00:06:28.858 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:06:28.858 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:06:28.878 -07 000000610 INF: ReagentValve: Recv 01\r 17-03-18 00:06:28.878 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 1 to 3. Moving 2 positions. 17-03-18 00:06:28.878 -07 000000610 INF: ReagentValve: Xmit +03\r 17-03-18 00:06:29.108 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:06:29.128 -07 000000610 INF: ReagentValve: Recv 01AC\r 17-03-18 00:06:29.128 -07 000000610 INF: ReagentValve: Move+ from 1 to 3 took 428 steps, expected 408. Error = 20 steps (4.9%) 17-03-18 00:06:29.178 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:06:29.198 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:06:29.198 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:06:29.298 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:06:29.298 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 20 µL, calling command Pumping to Flow Cell #1 17-03-18 00:06:29.298 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:29.338 -07 000000610 INF: LanePump: Recv /0`1770(0x03)\r\n 17-03-18 00:06:29.338 -07 000000610 INF: LanePump: Current Position of Pump is: 1770 17-03-18 00:06:29.338 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:06:29.468 -07 000000610 INF: LanePump: Xmit /1V400IP120R\r 17-03-18 00:06:29.508 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:06:30.338 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:06:30.378 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:30.408 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:30.408 -07 000000610 INF: LanePump: Waited 899 ms for pump motion to complete 17-03-18 00:06:30.408 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:06:30.538 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:30.568 -07 000000610 INF: LanePump: Recv /0`1890(0x03)\r\n 17-03-18 00:06:30.568 -07 000000610 INF: LanePump: Current Position of Pump is: 1890 17-03-18 00:06:30.698 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:06:30.728 -07 000000610 INF: LanePump: Recv /0`1890(0x03)\r\n 17-03-18 00:06:30.728 -07 000000610 INF: LanePump: Current Position of Pump is: 1890 17-03-18 00:06:30.728 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:06:30.858 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:06:30.888 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:31.318 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:06:31.348 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:06:31.348 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:06:31.348 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:06:31.348 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 65° =================== 17-03-18 00:06:31.348 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 65°, FC temperature to 65° =================== 17-03-18 00:06:31.348 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 65000000\n [FlowCellTempControl] 17-03-18 00:06:31.348 -07 000000458 INF: FPGA: Recv TEC2TEMP 17-03-18 00:06:31.358 -07 000000610 INF: FPGA: 'TEC2TEMP 0 65000000' command took 1 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:06:31.358 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:06:31.358 -07 000000478 INF: FPGA: Recv TEC2EN 17-03-18 00:06:31.368 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:06:31.368 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 65° =================== 17-03-18 00:06:31.368 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:06:31.368 -07 000000610 INF: WaitCommand(30000) start 17-03-18 00:06:32.128 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 0 17-03-18 00:06:32.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=1, Total=1. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:06:35.708 -07 000000482 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:06:37.128 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:06:37.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:06:41.088 -07 000000476 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:06:42.128 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:06:42.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:06:46.448 -07 000000476 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:06:47.128 -07 000000476 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:06:47.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:06:51.818 -07 000000448 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:06:52.128 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:06:52.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:06:57.128 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:06:57.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:06:57.198 -07 000000448 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:07:01.378 -07 000000610 INF: WaitCommand(30000) end 17-03-18 00:07:01.378 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:07:01.378 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 5 µL, calling command Pumping to Flow Cell #1 17-03-18 00:07:01.478 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:01.508 -07 000000610 INF: LanePump: Recv /0`1890(0x03)\r\n 17-03-18 00:07:01.508 -07 000000610 INF: LanePump: Current Position of Pump is: 1890 17-03-18 00:07:01.508 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:07:01.638 -07 000000610 INF: LanePump: Xmit /1V400IP30R\r 17-03-18 00:07:01.678 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:02.128 -07 000000586 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:02.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:07:02.218 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:02.248 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:02.248 -07 000000610 INF: LanePump: Waited 569 ms for pump motion to complete 17-03-18 00:07:02.248 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:07:02.378 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:02.408 -07 000000610 INF: LanePump: Recv /0`1920(0x03)\r\n 17-03-18 00:07:02.408 -07 000000610 INF: LanePump: Current Position of Pump is: 1920 17-03-18 00:07:02.538 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:02.558 -07 000000586 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:07:02.568 -07 000000610 INF: LanePump: Recv /0`1920(0x03)\r\n 17-03-18 00:07:02.568 -07 000000610 INF: LanePump: Current Position of Pump is: 1920 17-03-18 00:07:02.568 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:07:02.698 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:07:02.728 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:03.158 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:03.188 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:03.188 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:07:03.188 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:07:03.188 -07 000000576 INF: WaitCommand(30000) start 17-03-18 00:07:03.188 -07 000000586 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:07:03.188 -07 000000586 INF: =================== Dispense to DispenseAndWait, Dispensing port OutputPort =================== 17-03-18 00:07:04.408 -07 000000586 INF: AsyncDelayTimer waited 1214ms 17-03-18 00:07:04.408 -07 000000586 INF: LanePump: Xmit /1?\r 17-03-18 00:07:04.438 -07 000000586 INF: LanePump: Recv /0`1920(0x03)\r\n 17-03-18 00:07:04.438 -07 000000586 INF: LanePump: Current Position of Pump is: 1920 17-03-18 00:07:04.568 -07 000000586 INF: LanePump: Xmit /1V1500OA0R\r 17-03-18 00:07:04.608 -07 000000586 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:06.948 -07 000000586 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:06.978 -07 000000586 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:07.128 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:07.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:07:07.398 -07 000000586 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:07.428 -07 000000586 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:07.428 -07 000000586 INF: LanePump: Waited 2819 ms for pump motion to complete 17-03-18 00:07:07.428 -07 000000586 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:07:07.428 -07 000000586 INF: =================== =================== 17-03-18 00:07:07.928 -07 000000448 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:07:12.128 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:12.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:07:13.298 -07 000000448 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:07:17.128 -07 000000486 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:17.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:07:19.558 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.970374, 17-03-18 00:07:22.128 -07 000000448 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:22.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:07:24.918 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:07:27.128 -07 000000486 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:27.128 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:07:30.278 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:07:32.128 -07 000000627 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:32.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1305, R2=1356, Total=2661. Warn if over 250 bubbles per interval. 17-03-18 00:07:33.198 -07 000000576 INF: WaitCommand(30000) end 17-03-18 00:07:33.198 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 22° =================== 17-03-18 00:07:33.198 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 22°, FC temperature to 22° =================== 17-03-18 00:07:33.198 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 22000000\n [FlowCellTempControl] 17-03-18 00:07:33.208 -07 000000382 INF: FPGA: Recv TEC2TEMP 17-03-18 00:07:33.208 -07 000000610 INF: FPGA: 'TEC2TEMP 0 22000000' command took 2 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:07:33.208 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:07:33.208 -07 000000627 INF: FPGA: Recv TEC2EN 17-03-18 00:07:33.218 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:07:33.218 -07 000000610 INF: =================== Cycle #82, Flow Cell Temp 22° =================== 17-03-18 00:07:33.218 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:07:33.218 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:07:33.218 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:07:33.218 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:33.258 -07 000000610 INF: LanePump: Recv /0`0(0x03)\r\n 17-03-18 00:07:33.258 -07 000000610 INF: LanePump: Current Position of Pump is: 0 17-03-18 00:07:33.258 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:07:33.388 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:07:33.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:35.638 -07 000000586 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:07:36.558 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:37.418 -07 000000486 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 2 R1 1 17-03-18 00:07:37.418 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=1, R2=2, Total=3. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1306, R2=1358, Total=2664. Warn if over 250 bubbles per interval. 17-03-18 00:07:37.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:37.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:37.878 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:37.878 -07 000000610 INF: LanePump: Waited 4449 ms for pump motion to complete 17-03-18 00:07:37.878 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:07:38.008 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:38.038 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:07:38.038 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:07:38.168 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:38.198 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:07:38.198 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:07:38.198 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:07:38.328 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:07:38.358 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:38.788 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:38.818 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:38.818 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:07:38.818 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:07:40.038 -07 000000610 INF: AsyncDelayTimer waited 1219ms 17-03-18 00:07:40.038 -07 000000610 INF: ReagentValve: Go to position 2 - PumpToFlowcell start 17-03-18 00:07:40.038 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:07:40.058 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:07:40.058 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 3 to 2. Moving 1 positions. 17-03-18 00:07:40.058 -07 000000610 INF: ReagentValve: Xmit -02\r 17-03-18 00:07:40.208 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:07:40.228 -07 000000610 INF: ReagentValve: Recv 00E3\r 17-03-18 00:07:40.228 -07 000000610 INF: ReagentValve: Move- from 3 to 2 took 227 steps, expected 204. Error = 23 steps (11.3%) 17-03-18 00:07:40.278 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:07:40.298 -07 000000610 INF: ReagentValve: Recv 02\r 17-03-18 00:07:40.298 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:07:40.398 -07 000000610 INF: =================== Cycle #82, Pumping Reagent USM (2) =================== 17-03-18 00:07:40.398 -07 000000610 INF: LanePump: Pumping from 2, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 45 µL, calling command Pumping to Flow Cell #1 17-03-18 00:07:40.398 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:40.428 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:07:40.428 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:07:40.428 -07 000000610 INF: =================== Cycle #82, Pumping Reagent USM (2), Aspirating port InputPort =================== 17-03-18 00:07:40.558 -07 000000610 INF: LanePump: Xmit /1V400IP270R\r 17-03-18 00:07:40.598 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:40.998 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:07:42.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:42.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:42.138 -07 000000476 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 2 R1 3 17-03-18 00:07:42.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=3, R2=2, Total=5. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:07:42.488 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:42.518 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:42.518 -07 000000610 INF: LanePump: Waited 1919 ms for pump motion to complete 17-03-18 00:07:42.518 -07 000000610 INF: =================== Cycle #82, Pumping Reagent USM (2) =================== 17-03-18 00:07:42.648 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:42.678 -07 000000610 INF: LanePump: Recv /0`990(0x03)\r\n 17-03-18 00:07:42.678 -07 000000610 INF: LanePump: Current Position of Pump is: 990 17-03-18 00:07:42.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:42.838 -07 000000610 INF: LanePump: Recv /0`990(0x03)\r\n 17-03-18 00:07:42.838 -07 000000610 INF: LanePump: Current Position of Pump is: 990 17-03-18 00:07:42.838 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:07:42.968 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:07:42.998 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:43.428 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:43.458 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:43.458 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:07:43.458 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:07:44.678 -07 000000610 INF: AsyncDelayTimer waited 1219ms 17-03-18 00:07:44.678 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:07:44.678 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:07:44.698 -07 000000610 INF: ReagentValve: Recv 02\r 17-03-18 00:07:44.698 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 2 to 3. Moving 1 positions. 17-03-18 00:07:44.698 -07 000000610 INF: ReagentValve: Xmit +03\r 17-03-18 00:07:44.848 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:07:44.868 -07 000000610 INF: ReagentValve: Recv 00E3\r 17-03-18 00:07:44.868 -07 000000610 INF: ReagentValve: Move+ from 2 to 3 took 227 steps, expected 204. Error = 23 steps (11.3%) 17-03-18 00:07:44.918 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:07:44.938 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:07:44.938 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:07:45.038 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:07:45.038 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 20 µL, calling command Pumping to Flow Cell #1 17-03-18 00:07:45.038 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:45.068 -07 000000610 INF: LanePump: Recv /0`990(0x03)\r\n 17-03-18 00:07:45.068 -07 000000610 INF: LanePump: Current Position of Pump is: 990 17-03-18 00:07:45.068 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:07:45.198 -07 000000610 INF: LanePump: Xmit /1V400IP120R\r 17-03-18 00:07:45.238 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:46.108 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:46.138 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:46.138 -07 000000610 INF: LanePump: Waited 899 ms for pump motion to complete 17-03-18 00:07:46.138 -07 000000610 INF: =================== Cycle #82, Pumping Reagent PR2 (3) =================== 17-03-18 00:07:46.268 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:46.298 -07 000000610 INF: LanePump: Recv /0`1110(0x03)\r\n 17-03-18 00:07:46.298 -07 000000610 INF: LanePump: Current Position of Pump is: 1110 17-03-18 00:07:46.358 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:07:46.428 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:07:46.458 -07 000000610 INF: LanePump: Recv /0`1110(0x03)\r\n 17-03-18 00:07:46.458 -07 000000610 INF: LanePump: Current Position of Pump is: 1110 17-03-18 00:07:46.458 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:07:46.588 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:07:46.618 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:47.048 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:47.078 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:47.078 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:07:47.078 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:07:47.078 -07 000000476 INF: WaitCommand(10000) start 17-03-18 00:07:47.078 -07 000000486 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:07:47.078 -07 000000486 INF: =================== Dispense to DispenseAndWait, Dispensing port OutputPort =================== 17-03-18 00:07:47.138 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:47.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:07:48.298 -07 000000486 INF: AsyncDelayTimer waited 1216ms 17-03-18 00:07:48.298 -07 000000486 INF: LanePump: Xmit /1?\r 17-03-18 00:07:48.328 -07 000000486 INF: LanePump: Recv /0`1110(0x03)\r\n 17-03-18 00:07:48.328 -07 000000486 INF: LanePump: Current Position of Pump is: 1110 17-03-18 00:07:48.458 -07 000000486 INF: LanePump: Xmit /1V1500OA0R\r 17-03-18 00:07:48.498 -07 000000486 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:50.028 -07 000000486 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:50.058 -07 000000486 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:07:50.478 -07 000000486 INF: LanePump: Xmit /1Q\r 17-03-18 00:07:50.508 -07 000000486 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:07:50.508 -07 000000486 INF: LanePump: Waited 2009 ms for pump motion to complete 17-03-18 00:07:50.508 -07 000000486 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:07:50.508 -07 000000486 INF: =================== =================== 17-03-18 00:07:51.718 -07 000000448 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:07:52.138 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:52.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:07:57.078 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 00:07:57.088 -07 000000476 INF: WaitCommand(10000) end 17-03-18 00:07:57.088 -07 000000610 INF: =================== Cycle #82, Imaging =================== 17-03-18 00:07:57.088 -07 000000610 INF: Imaging Cycle 82: ScanTilesCommand Cycle 82 time start. 17-03-18 00:07:57.088 -07 000000610 INF: ReagentValve: Go to position 24 (Plug) - Start of ScanTilesCommand 17-03-18 00:07:57.088 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:07:57.118 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:07:57.118 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 3 to 24. Moving 3 positions. 17-03-18 00:07:57.118 -07 000000610 INF: ReagentValve: Xmit -18\r 17-03-18 00:07:57.648 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:07:57.648 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:07:57.668 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:07:57.688 -07 000000610 INF: ReagentValve: Recv 0278\r 17-03-18 00:07:57.688 -07 000000610 INF: ReagentValve: Move- from 3 to 24 took 632 steps, expected 612. Error = 20 steps (3.3%) 17-03-18 00:07:57.738 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:07:57.758 -07 000000610 INF: ReagentValve: Recv 18\r 17-03-18 00:07:57.758 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:07:57.758 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:07:57.758 -07 000000586 INF: RedLED: Snapping RedLED at power = 1930.000 mW, snap mode for ~0 ms start. 17-03-18 00:07:57.758 -07 000000586 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:07:57.758 -07 000000586 INF: FPGA: Xmit EXLED1ON\n [RedLED] 17-03-18 00:07:57.758 -07 000000478 INF: FPGA: Recv EXLED1ON 17-03-18 00:07:57.768 -07 000000586 INF: FPGA: 'EXLED1ON' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:07:57.768 -07 000000586 INF: FPGA: Xmit EXLED1ADCR\n [RedLED] 17-03-18 00:07:57.768 -07 000000478 INF: FPGA: Recv EXLED1ADCR 23230 17-03-18 00:07:57.778 -07 000000586 INF: FPGA: 'EXLED1ADCR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:07:57.778 -07 000000586 INF: RedLED: Using Red data: Photodiode ADC 23230 --> 7.5 mW (old method), or 205.9 mW (new method). Difference = 198.4 mW 17-03-18 00:07:57.778 -07 000000586 INF: FPGA: Xmit EXLED1OFF\n [RedLED] 17-03-18 00:07:57.778 -07 000000627 INF: FPGA: Recv EXLED1OFF 17-03-18 00:07:57.788 -07 000000586 INF: FPGA: 'EXLED1OFF' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:07:57.788 -07 000000586 INF: RedLED: LED was on for 20 ms 17-03-18 00:07:57.788 -07 000000586 INF: RedLED: Snapping RedLED at power = 1930.000 mW, snap mode for ~0 ms end. Duration was 24 ms. 17-03-18 00:07:57.788 -07 000000586 INF: GreenLED: Snapping GreenLED at power = 5964.000 mW, snap mode for ~0 ms start. 17-03-18 00:07:57.788 -07 000000586 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:07:57.788 -07 000000586 INF: FPGA: Xmit EXLED2ON\n [GreenLED] 17-03-18 00:07:57.788 -07 000000482 INF: FPGA: Recv EXLED2ON 17-03-18 00:07:57.798 -07 000000586 INF: FPGA: 'EXLED2ON' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:07:57.798 -07 000000586 INF: FPGA: Xmit EXLED1ADCR\n [GreenLED] 17-03-18 00:07:57.798 -07 000000482 INF: FPGA: Recv EXLED1ADCR 2093 17-03-18 00:07:57.808 -07 000000586 INF: FPGA: 'EXLED1ADCR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:07:57.808 -07 000000586 INF: GreenLED: Using Green data: Photodiode ADC 2093 --> 0.9 mW (old method), or 181.9 mW (new method). Difference = 181.0 mW 17-03-18 00:07:57.808 -07 000000586 INF: FPGA: Xmit EXLED2OFF\n [GreenLED] 17-03-18 00:07:57.808 -07 000000486 INF: FPGA: Recv EXLED2OFF 17-03-18 00:07:57.818 -07 000000586 INF: FPGA: 'EXLED2OFF' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:07:57.818 -07 000000586 INF: GreenLED: LED was on for 21 ms 17-03-18 00:07:57.818 -07 000000586 INF: GreenLED: Snapping GreenLED at power = 5964.000 mW, snap mode for ~0 ms end. Duration was 29 ms. 17-03-18 00:07:57.818 -07 000000610 INF: FPGA: Xmit EMCUR 80\n [Compensator] 17-03-18 00:07:57.818 -07 000000586 INF: FPGA: Recv EMCUR 17-03-18 00:07:57.828 -07 000000610 INF: FPGA: 'EMCUR 80' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:07:57.828 -07 000000610 INF: FPGA: Xmit EMOUT 20 70\n [Compensator] 17-03-18 00:07:58.328 -07 000000482 INF: FPGA: Recv EMOUT 17-03-18 00:07:58.328 -07 000000610 INF: FPGA: 'EMOUT 20 70' command took 0 ms to send, 493 ms to get response, and 502 ms overall 17-03-18 00:07:58.328 -07 000000610 INF: FPGA: Xmit EMCUR 75\n [Compensator] 17-03-18 00:07:58.328 -07 000000586 INF: FPGA: Recv EMCUR 17-03-18 00:07:58.338 -07 000000610 INF: FPGA: 'EMCUR 75' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:07:58.338 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 0 - Best Z for this model is: -0.172880 17-03-18 00:07:58.338 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 1 at Y-position 75.380859375, Z-Position -0.12606659766587. ------ 17-03-18 00:07:58.338 -07 000000448 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:07:58.338 -07 000000627 INF: Y Motor: MoveYZ optimized starting. Moving Y to 59.9126 mm, moving Z to -0.17846 mm 17-03-18 00:07:58.338 -07 000000627 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:07:58.338 -07 000000547 INF: Y Motor: Entering MoveAbsStepped (target = 95860 steps, sync = True, waitForSettling = True) 17-03-18 00:07:58.338 -07 000000547 INF: Y Motor: Moving to 59.9126 mm, started at 75.3809 mm, delta = -15.4683 mm 17-03-18 00:07:58.338 -07 000000478 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:07:58.348 -07 000000448 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:07:58.348 -07 000000547 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:07:58.348 -07 000000482 INF: Z Motor: Entering MoveAbsStepped (DAC = 38985, sync = True, waitForSettling = False) 17-03-18 00:07:58.348 -07 000000482 INF: Z Motor: Moving to -0.178462 mm, started at -0.126067 mm, distance = -52.40 um 17-03-18 00:07:58.348 -07 000000486 INF: FPGA: Recv YRDPOS 154380 17-03-18 00:07:58.358 -07 000000547 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:07:58.358 -07 000000547 INF: Y Motor: Encoder = 154380 = motor step 120609.4 = 75.3809 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:07:58.358 -07 000000547 INF: Y Motor: Last commanded position = 75.3806 mm (120608.9 steps), delta from last commanded position = -15.4680 mm (-24748.8 steps). UsingTableMove = False 17-03-18 00:07:58.358 -07 000000547 INF: Y Motor: Moving to 122701 encoder counts 17-03-18 00:07:58.358 -07 000000448 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:07:58.368 -07 000000482 INF: FPGA: Xmit ZDACW 38985\n [Z Motor] 17-03-18 00:07:58.368 -07 000000478 INF: FPGA: Recv ZDACW 17-03-18 00:07:58.378 -07 000000547 INF: FPGA: Xmit YMVTO 122701\n [Y Motor] 17-03-18 00:07:58.378 -07 000000482 INF: FPGA: 'ZDACW 38985' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:07:58.388 -07 000000482 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:07:58.388 -07 000000486 INF: FPGA: Recv ZDACR 38985 17-03-18 00:07:58.398 -07 000000482 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:07:58.398 -07 000000482 INF: Z Motor: MoveAbsStepped took 49 ms (did NOT wait for settling yet)) 17-03-18 00:07:58.558 -07 000000586 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:07:58.558 -07 000000448 INF: FPGA: 'FOCMRRFOC' command took 1 ms to send, 193 ms to get response, and 213 ms overall 17-03-18 00:08:00.058 -07 000000482 INF: FPGA: Recv YMVTO 122703 24776 17-03-18 00:08:00.058 -07 000000547 INF: FPGA: 'YMVTO 122701' command took 0 ms to send, 1676 ms to get response, and 1705 ms overall 17-03-18 00:08:00.058 -07 000000547 INF: Y Motor: Position error before settling = 2 encoder counts (0.001 mm) 17-03-18 00:08:00.058 -07 000000547 INF: Y Motor: To move from 75.381 to 59.913 (-15.468 mm) should take 24749 steps, but required 24776 steps (15.485 mm). Slippage = 0.017 mm (0.00%). 17-03-18 00:08:00.058 -07 000000547 INF: Y Motor: FPGA move command 'YMVTO 122701' took 1705 ms, distance = 15.468 mm, average velocity = 9.072 mm/sec 17-03-18 00:08:02.138 -07 000000586 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:02.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:02.438 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:08:02.908 -07 000000547 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:02.908 -07 000000448 INF: FPGA: Recv YRDPOS 122698 17-03-18 00:08:02.918 -07 000000547 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:02.918 -07 000000547 INF: Y Motor: Encoder = 122698 = motor step 95857.8 = 59.9111 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:02.938 -07 000000547 INF: Y Motor: After move to 59.912609, error = -0.001476, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:02.938 -07 000000547 INF: Y Motor: Position error after settling = -3 encoder counts (-0.001 mm), Delta during settling = -5 counts (-0.002 mm) 17-03-18 00:08:02.938 -07 000000547 INF: Y Motor: Moved to 59.9111 after 4598 ms 17-03-18 00:08:02.948 -07 000000627 INF: Z Motor: EndOfMoveAbsStepped: target = -0.178462 mm (DAC = 38985) 17-03-18 00:08:02.948 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:02.948 -07 000000448 INF: FPGA: Recv ZSGSRD 37068 17-03-18 00:08:02.958 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:02.958 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:02.958 -07 000000482 INF: FPGA: Recv ZSGSRD 37070 17-03-18 00:08:02.968 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:02.968 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:02.968 -07 000000448 INF: FPGA: Recv ZSGSRD 37072 17-03-18 00:08:02.978 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:02.978 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:02.978 -07 000000382 INF: FPGA: Recv ZSGSRD 37069 17-03-18 00:08:02.988 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:02.988 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:02.988 -07 000000486 INF: FPGA: Recv ZSGSRD 37066 17-03-18 00:08:02.998 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:02.998 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:02.998 -07 000000448 INF: FPGA: Recv ZSGSRD 37076 17-03-18 00:08:03.008 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.008 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:03.008 -07 000000586 INF: FPGA: Recv ZSGSRD 37071 17-03-18 00:08:03.018 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.018 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:03.018 -07 000000382 INF: FPGA: Recv ZSGSRD 37068 17-03-18 00:08:03.028 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.028 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:03.028 -07 000000482 INF: FPGA: Recv ZSGSRD 37068 17-03-18 00:08:03.038 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.038 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:03.038 -07 000000382 INF: FPGA: Recv ZSGSRD 37073 17-03-18 00:08:03.048 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.048 -07 000000627 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:03.048 -07 000000586 INF: FPGA: Recv ZPIEZORD 33232 17-03-18 00:08:03.058 -07 000000627 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.058 -07 000000627 INF: Z Motor: Piezo is near 51.0 V (nominally 0 to 100 V), expect near 59.5 V, since DAC is at 5.949 V. Piezo Error = -8.5 V. 17-03-18 00:08:03.058 -07 000000627 INF: Z Motor: ReadAdcAverage: Commanded Z = -178.5 um, Read 10 ADC samples: Avg = 37070 (-177.6 uncal um), Error = 0.9 uncal um, Median = 37070 (-177.6 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 37066 (-177.5 uncal um), Max = 37076 (-177.6 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:08:03.058 -07 000000627 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 37070, posNoise = 6, negNoise = 4 17-03-18 00:08:03.058 -07 000000627 INF: Z Motor: After move to -0.178462, error = -0.000007, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:03.058 -07 000000627 INF: Z Motor: Settle wait time = 4599 ms, measure time = 110 ms (allowed 150 ms), total time = 4709 ms 17-03-18 00:08:03.058 -07 000000627 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:03.098 -07 000000627 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 4755 ms 17-03-18 00:08:03.098 -07 000000627 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:03.098 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:03.098 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:03.098 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:03.098 -07 000000547 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:03.098 -07 000000482 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:08:03.098 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:03.098 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:03.098 -07 000000482 INF: Camera: sBCa_SetPowerMode(785074288, 1) start. 17-03-18 00:08:03.128 -07 000000482 INF: Camera: sBCa_SetPowerMode(785074288, 1) end. Duration was 23 ms. 17-03-18 00:08:03.128 -07 000000482 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:08:03.128 -07 000000482 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 4 ms. 17-03-18 00:08:03.128 -07 000000482 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:08:03.148 -07 000000482 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 13 ms. 17-03-18 00:08:03.148 -07 000000482 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 43 ms. 17-03-18 00:08:03.148 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:08:03.188 -07 000000586 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:08:03.488 -07 000000382 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:08:03.488 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:08:03.488 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:08:03.498 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1441468064, 0x0, 559484976) start. 17-03-18 00:08:03.528 -07 000000476 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:08:03.528 -07 000000486 INF: FPGA: Recv CAMSNAP 17-03-18 00:08:03.528 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 377 ms to get response, and 384 ms overall 17-03-18 00:08:03.618 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1441468064, 0x0, 559484976) end. Duration was 126 ms. 17-03-18 00:08:03.618 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:03.618 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:08:03.618 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 129 ms. 17-03-18 00:08:03.728 -07 000000448 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:08:03.728 -07 000000382 INF: Image SNR check: start 17-03-18 00:08:03.728 -07 000000486 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:08:03.738 -07 000000448 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:08:03.738 -07 000000448 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:08:03.738 -07 000000382 INF: Image SNR check: pass [peak (Max-Min)=48.48] 17-03-18 00:08:03.738 -07 000000382 INF: Autofocus start 17-03-18 00:08:03.738 -07 000000382 INF: MeasureProfile start 17-03-18 00:08:03.748 -07 000000382 INF: MeasureProfile done 17-03-18 00:08:03.748 -07 000000382 INF: X=0 Z correction [um]=-0.0713522 17-03-18 00:08:03.748 -07 000000382 INF: Autofocus finished 17-03-18 00:08:03.938 -07 000000382 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:08:03.938 -07 000000448 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:08:03.938 -07 000000610 INF: ------------ Scan Snapping tile 1 at Y-position 59.9111328125, Z-position -0.178468896106214. ---------- 17-03-18 00:08:03.938 -07 000000610 INF: Cycle 82 Tile 1 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:03.948 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 37482, sync = True, waitForSettling = True) 17-03-18 00:08:03.948 -07 000000610 INF: Z Motor: Moving to -0.171582 mm, started at -0.178469 mm, distance = 6.89 um 17-03-18 00:08:03.948 -07 000000610 INF: FPGA: Xmit ZDACW 37482\n [Z Motor] 17-03-18 00:08:03.948 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:08:03.958 -07 000000610 INF: FPGA: 'ZDACW 37482' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.958 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:03.958 -07 000000547 INF: FPGA: Recv ZDACR 37482 17-03-18 00:08:03.968 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:03.968 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:04.118 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.118 -07 000000486 INF: FPGA: Recv ZSGSRD 36000 17-03-18 00:08:04.128 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:04.128 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.128 -07 000000476 INF: FPGA: Recv ZSGSRD 36000 17-03-18 00:08:04.138 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.138 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.138 -07 000000382 INF: FPGA: Recv ZSGSRD 36000 17-03-18 00:08:04.148 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.148 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.148 -07 000000382 INF: FPGA: Recv ZSGSRD 36000 17-03-18 00:08:04.158 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.158 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.158 -07 000000482 INF: FPGA: Recv ZSGSRD 36000 17-03-18 00:08:04.168 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.168 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.168 -07 000000476 INF: FPGA: Recv ZSGSRD 36001 17-03-18 00:08:04.178 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.178 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.178 -07 000000486 INF: FPGA: Recv ZSGSRD 36004 17-03-18 00:08:04.188 -07 000000610 INF: FPGA: 'ZSGSRD' command took 1 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.188 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.188 -07 000000547 INF: FPGA: Recv ZSGSRD 35998 17-03-18 00:08:04.198 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.198 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.198 -07 000000448 INF: FPGA: Recv ZSGSRD 35999 17-03-18 00:08:04.208 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.208 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:04.208 -07 000000586 INF: FPGA: Recv ZSGSRD 35996 17-03-18 00:08:04.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.218 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:04.218 -07 000000547 INF: FPGA: Recv ZPIEZORD 31812 17-03-18 00:08:04.228 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:04.228 -07 000000610 INF: Z Motor: Piezo is near 48.0 V (nominally 0 to 100 V), expect near 57.2 V, since DAC is at 5.719 V. Piezo Error = -9.2 V. 17-03-18 00:08:04.228 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -171.6 um, Read 10 ADC samples: Avg = 36000 (-170.7 uncal um), Error = 0.9 uncal um, Median = 36000 (-170.7 uncal um), StdDev = 2 (0.0 uncal um), Range = 8 (0.1 uncal um), Min = 35996 (-170.7 uncal um), Max = 36004 (-170.7 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:08:04.228 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 36000, posNoise = 4, negNoise = 4 17-03-18 00:08:04.228 -07 000000610 INF: Z Motor: After move to -0.171582, error = -0.000013, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:04.228 -07 000000610 INF: Z Motor: MoveAbsStepped took 281 ms) 17-03-18 00:08:04.228 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:04.228 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:04.228 -07 000000586 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:04.228 -07 000000448 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:04.228 -07 000000382 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:04.228 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:04.228 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:04.228 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:04.228 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:08:04.228 -07 000000382 INF: Camera: sBCa_SetPowerMode(785074704, 1) start. 17-03-18 00:08:04.228 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:04.228 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:04.228 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:08:04.228 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:04.248 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:08:04.248 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:08:04.248 -07 000000382 INF: Camera: sBCa_SetPowerMode(785074704, 1) end. Duration was 22 ms. 17-03-18 00:08:04.248 -07 000000382 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:04.258 -07 000000382 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 2 ms. 17-03-18 00:08:04.258 -07 000000382 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:04.268 -07 000000382 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 12 ms. 17-03-18 00:08:04.268 -07 000000382 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 39 ms. 17-03-18 00:08:04.268 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 37658 150\n [Camera] 17-03-18 00:08:04.468 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:05.508 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:05.508 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:05.508 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:05.508 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:05.508 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:05.508 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:05.648 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:05.648 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1456144144, 0x0, 391868352) start. 17-03-18 00:08:05.648 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:05.648 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 2147487792, 0x0, 418435064) start. 17-03-18 00:08:05.708 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 37658 1425 17-03-18 00:08:05.848 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:06.028 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 2147487792, 0x0, 418435064) end. Duration was 383 ms. 17-03-18 00:08:06.028 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 383 ms. 17-03-18 00:08:06.028 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:06.038 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:06.038 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1456144144, 0x0, 391868352) end. Duration was 388 ms. 17-03-18 00:08:06.038 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 388 ms. 17-03-18 00:08:06.038 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:06.038 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:06.038 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 528 ms. 17-03-18 00:08:06.038 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 528 ms. 17-03-18 00:08:06.348 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:06.348 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:06.348 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:06.358 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:06.358 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:06.358 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:06.358 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:06.358 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:08:06.358 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:06.358 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:08:06.758 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 403 ms. 17-03-18 00:08:06.758 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 404 ms. 17-03-18 00:08:06.758 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:06.768 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 406 ms. 17-03-18 00:08:06.768 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 407 ms. 17-03-18 00:08:06.768 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:06.768 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:08:06.768 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 416 ms. 17-03-18 00:08:06.768 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:06.778 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 419 ms. 17-03-18 00:08:06.778 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:06.778 -07 000000486 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:06.778 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 37658 150' command took 2 ms to send, 2495 ms to get response, and 2507 ms overall 17-03-18 00:08:06.778 -07 000000610 INF: Skipping AutoFocus in Tile 2: adopting Best-Z from the cache: -0.171673 17-03-18 00:08:06.778 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 60.7426 mm, moving Z to -0.17167 mm 17-03-18 00:08:06.778 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:06.788 -07 000000486 INF: Y Motor: Entering MoveAbsStepped (target = 97188 steps, sync = True, waitForSettling = True) 17-03-18 00:08:06.788 -07 000000486 INF: Y Motor: Moving to 60.7426 mm, started at 59.9111 mm, delta = 0.8315 mm 17-03-18 00:08:06.788 -07 000000482 INF: Z Motor: Entering MoveAbsStepped (DAC = 37502, sync = True, waitForSettling = False) 17-03-18 00:08:06.788 -07 000000482 INF: Z Motor: Moving to -0.171673 mm, started at -0.171595 mm, distance = -0.08 um 17-03-18 00:08:06.798 -07 000000482 INF: FPGA: Xmit ZDACW 37502\n [Z Motor] 17-03-18 00:08:06.808 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:07.668 -07 000000478 INF: FPGA: Recv ZDACW 17-03-18 00:08:07.668 -07 000000478 INF: FPGA: Recv YRDPOS 122698 17-03-18 00:08:07.668 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:07.668 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 850 ms to get response, and 877 ms overall 17-03-18 00:08:07.668 -07 000000486 INF: Y Motor: Encoder = 122698 = motor step 95857.8 = 59.9111 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:07.668 -07 000000486 INF: Y Motor: Last commanded position = 59.9126 mm (95860.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:07.668 -07 000000486 INF: Y Motor: Moving 1330 steps2 17-03-18 00:08:07.668 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:07.668 -07 000000482 INF: FPGA: 'ZDACW 37502' command took 0 ms to send, 860 ms to get response, and 876 ms overall 17-03-18 00:08:07.688 -07 000000482 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:07.698 -07 000000486 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:08.198 -07 000000627 INF: FPGA: Recv ZDACR 37502 17-03-18 00:08:08.198 -07 000000627 INF: FPGA: Recv YMVTILE 17-03-18 00:08:08.198 -07 000000482 INF: FPGA: 'ZDACR' command took 0 ms to send, 500 ms to get response, and 528 ms overall 17-03-18 00:08:08.198 -07 000000482 INF: Z Motor: MoveAbsStepped took 1406 ms (did NOT wait for settling yet)) 17-03-18 00:08:08.198 -07 000000486 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 490 ms to get response, and 528 ms overall 17-03-18 00:08:08.198 -07 000000627 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:08:08.238 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:08.238 -07 000000482 INF: FPGA: Recv YRDPOS 124381 17-03-18 00:08:08.248 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:08.248 -07 000000486 INF: Y Motor: Encoder = 124381 = motor step 97172.7 = 60.7329 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:08.248 -07 000000486 INF: Y Motor: Position error before settling (table move) = -20 encoder counts (-0.010 mm) 17-03-18 00:08:08.248 -07 000000486 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 529 ms, distance = 0.831 mm, average velocity = 1.572 mm/sec 17-03-18 00:08:09.198 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:09.198 -07 000000482 INF: FPGA: Recv YRDPOS 124381 17-03-18 00:08:09.208 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:09.208 -07 000000486 INF: Y Motor: Encoder = 124381 = motor step 97172.7 = 60.7329 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:09.228 -07 000000486 INF: Y Motor: After move to 60.742607, error = -0.009697, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:09.228 -07 000000486 INF: Y Motor: Position error after settling (table move) = -20 encoder counts (-0.010 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:09.228 -07 000000486 INF: Y Motor: Moved to 60.7329 after 2445 ms (table move) 17-03-18 00:08:09.238 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.171673 mm (DAC = 37502) 17-03-18 00:08:09.238 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.238 -07 000000476 INF: FPGA: Recv ZSGSRD 36016 17-03-18 00:08:09.248 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:09.248 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.248 -07 000000627 INF: FPGA: Recv ZSGSRD 36017 17-03-18 00:08:09.258 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.258 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.258 -07 000000586 INF: FPGA: Recv ZSGSRD 36012 17-03-18 00:08:09.268 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.268 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.268 -07 000000382 INF: FPGA: Recv ZSGSRD 36013 17-03-18 00:08:09.278 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.278 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.278 -07 000000486 INF: FPGA: Recv ZSGSRD 36012 17-03-18 00:08:09.288 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.288 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.288 -07 000000382 INF: FPGA: Recv ZSGSRD 36008 17-03-18 00:08:09.298 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.298 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.298 -07 000000586 INF: FPGA: Recv ZSGSRD 36016 17-03-18 00:08:09.308 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.308 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.308 -07 000000382 INF: FPGA: Recv ZSGSRD 36011 17-03-18 00:08:09.318 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.318 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.318 -07 000000482 INF: FPGA: Recv ZSGSRD 36020 17-03-18 00:08:09.328 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.328 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.328 -07 000000486 INF: FPGA: Recv ZSGSRD 36018 17-03-18 00:08:09.338 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.338 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:09.338 -07 000000476 INF: FPGA: Recv ZPIEZORD 31817 17-03-18 00:08:09.348 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.348 -07 000000610 INF: Z Motor: Piezo is near 48.0 V (nominally 0 to 100 V), expect near 57.2 V, since DAC is at 5.722 V. Piezo Error = -9.3 V. 17-03-18 00:08:09.348 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -171.7 um, Read 10 ADC samples: Avg = 36014 (-170.8 uncal um), Error = 0.9 uncal um, Median = 36014 (-170.8 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 36008 (-170.8 uncal um), Max = 36020 (-170.8 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:08:09.348 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 36014, posNoise = 6, negNoise = 6 17-03-18 00:08:09.348 -07 000000610 INF: Z Motor: After move to -0.171673, error = -0.000012, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:09.348 -07 000000610 INF: Z Motor: Settle wait time = 2447 ms, measure time = 110 ms (allowed 150 ms), total time = 2557 ms 17-03-18 00:08:09.348 -07 000000610 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:09.388 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 2601 ms 17-03-18 00:08:09.388 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:09.388 -07 000000610 INF: ------------ Scan Snapping tile 2 at Y-position 60.73291015625, Z-position -0.171684928084514. ---------- 17-03-18 00:08:09.388 -07 000000610 INF: Cycle 82 Tile 2 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:09.388 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 37325, sync = True, waitForSettling = True) 17-03-18 00:08:09.388 -07 000000610 INF: Z Motor: Moving to -0.170863 mm, started at -0.171685 mm, distance = 0.82 um 17-03-18 00:08:09.388 -07 000000610 INF: FPGA: Xmit ZDACW 37325\n [Z Motor] 17-03-18 00:08:09.388 -07 000000486 INF: FPGA: Recv ZDACW 17-03-18 00:08:09.398 -07 000000610 INF: FPGA: 'ZDACW 37325' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:08:09.398 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:09.398 -07 000000627 INF: FPGA: Recv ZDACR 37325 17-03-18 00:08:09.408 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.408 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:09.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.558 -07 000000486 INF: FPGA: Recv ZSGSRD 35893 17-03-18 00:08:09.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:09.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.568 -07 000000586 INF: FPGA: Recv ZSGSRD 35887 17-03-18 00:08:09.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.578 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.578 -07 000000482 INF: FPGA: Recv ZSGSRD 35888 17-03-18 00:08:09.588 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.588 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.588 -07 000000586 INF: FPGA: Recv ZSGSRD 35889 17-03-18 00:08:09.598 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.598 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.598 -07 000000476 INF: FPGA: Recv ZSGSRD 35891 17-03-18 00:08:09.608 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.608 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.608 -07 000000448 INF: FPGA: Recv ZSGSRD 35888 17-03-18 00:08:09.618 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.618 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.618 -07 000000476 INF: FPGA: Recv ZSGSRD 35883 17-03-18 00:08:09.628 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.628 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.628 -07 000000586 INF: FPGA: Recv ZSGSRD 35890 17-03-18 00:08:09.638 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.638 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.638 -07 000000486 INF: FPGA: Recv ZSGSRD 35889 17-03-18 00:08:09.648 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.648 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:09.648 -07 000000586 INF: FPGA: Recv ZSGSRD 35887 17-03-18 00:08:09.658 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.658 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:09.658 -07 000000482 INF: FPGA: Recv ZPIEZORD 31629 17-03-18 00:08:09.668 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:09.668 -07 000000610 INF: Z Motor: Piezo is near 47.6 V (nominally 0 to 100 V), expect near 57.0 V, since DAC is at 5.695 V. Piezo Error = -9.4 V. 17-03-18 00:08:09.668 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -170.9 um, Read 10 ADC samples: Avg = 35888 (-170.0 uncal um), Error = 0.9 uncal um, Median = 35888 (-170.0 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 35883 (-170.0 uncal um), Max = 35893 (-170.0 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:08:09.668 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35888, posNoise = 5, negNoise = 5 17-03-18 00:08:09.668 -07 000000610 INF: Z Motor: After move to -0.170863, error = -0.000013, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:09.668 -07 000000610 INF: Z Motor: MoveAbsStepped took 276 ms) 17-03-18 00:08:09.668 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:09.668 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:09.668 -07 000000486 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:09.668 -07 000000382 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:09.668 -07 000000586 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:09.668 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:09.668 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:09.668 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:09.668 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:09.668 -07 000000586 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:09.668 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:08:09.668 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:09.678 -07 000000586 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:09.678 -07 000000586 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:09.678 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:08:09.678 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:09.688 -07 000000586 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:08:09.688 -07 000000586 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:08:09.688 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:08:09.688 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:08:09.698 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 37502 150\n [Camera] 17-03-18 00:08:09.898 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:10.928 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:10.928 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:10.938 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:10.938 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:10.938 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 2182910056, 0x0, 391868352) start. 17-03-18 00:08:10.938 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:10.938 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:10.938 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:10.938 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:10.938 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 2218332320, 0x0, 418435064) start. 17-03-18 00:08:11.128 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 37502 1425 17-03-18 00:08:11.278 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:11.338 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 2182910056, 0x0, 391868352) end. Duration was 400 ms. 17-03-18 00:08:11.338 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:08:11.338 -07 000000476 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:11.338 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 2218332320, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:08:11.338 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:08:11.338 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:11.338 -07 000000476 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:08:11.338 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 406 ms. 17-03-18 00:08:11.338 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:11.348 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:08:11.778 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:11.778 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:11.778 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:11.778 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:11.778 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) start. 17-03-18 00:08:11.778 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:11.778 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:11.778 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:11.898 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:11.898 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:08:11.968 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:11.968 -07 000000586 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:11.968 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 37502 150' command took 2 ms to send, 2262 ms to get response, and 2272 ms overall 17-03-18 00:08:11.968 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 0 - Best Z for this model is: -0.172880 17-03-18 00:08:11.968 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 3 at Y-position 60.73291015625, Z-Position -0.170875477354652. ------ 17-03-18 00:08:11.968 -07 000000482 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:08:11.968 -07 000000476 INF: Y Motor: MoveYZ optimized starting. Moving Y to 61.5726 mm, moving Z to -0.17741 mm 17-03-18 00:08:11.968 -07 000000476 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:11.978 -07 000000586 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:08:11.978 -07 000000482 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 2 ms to get response, and 3 ms overall 17-03-18 00:08:11.978 -07 000000627 INF: Y Motor: Entering MoveAbsStepped (target = 98516 steps, sync = True, waitForSettling = True) 17-03-18 00:08:11.978 -07 000000627 INF: Y Motor: Moving to 61.5726 mm, started at 60.7329 mm, delta = 0.8397 mm 17-03-18 00:08:11.978 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:11.978 -07 000000486 INF: Z Motor: Entering MoveAbsStepped (DAC = 38755, sync = True, waitForSettling = False) 17-03-18 00:08:11.978 -07 000000486 INF: Z Motor: Moving to -0.177409 mm, started at -0.170875 mm, distance = -6.53 um 17-03-18 00:08:11.978 -07 000000478 INF: FPGA: Recv YRDPOS 124381 17-03-18 00:08:11.988 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:08:11.988 -07 000000627 INF: Y Motor: Encoder = 124381 = motor step 97172.7 = 60.7329 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:11.988 -07 000000627 INF: Y Motor: Last commanded position = 60.7426 mm (97188.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:11.988 -07 000000627 INF: Y Motor: Moving 1344 steps-20 17-03-18 00:08:11.988 -07 000000482 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:08:11.998 -07 000000486 INF: FPGA: Xmit ZDACW 38755\n [Z Motor] 17-03-18 00:08:11.998 -07 000000012 INF: FPGA: Recv ZDACW 17-03-18 00:08:12.008 -07 000000627 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:12.008 -07 000000486 INF: FPGA: 'ZDACW 38755' command took 0 ms to send, 0 ms to get response, and 27 ms overall 17-03-18 00:08:12.018 -07 000000486 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:12.018 -07 000000586 INF: FPGA: Recv ZDACR 38755 17-03-18 00:08:12.028 -07 000000486 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:12.028 -07 000000486 INF: Z Motor: MoveAbsStepped took 47 ms (did NOT wait for settling yet)) 17-03-18 00:08:12.138 -07 000000486 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:12.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:12.168 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) end. Duration was 390 ms. 17-03-18 00:08:12.168 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 391 ms. 17-03-18 00:08:12.168 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:12.168 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 2 ms. 17-03-18 00:08:12.168 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:08:12.278 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 379 ms. 17-03-18 00:08:12.278 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 379 ms. 17-03-18 00:08:12.278 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:12.288 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:12.288 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 513 ms. 17-03-18 00:08:13.218 -07 000000486 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:08:13.218 -07 000000486 INF: FPGA: Recv YMVTILE 17-03-18 00:08:13.218 -07 000000482 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 1223 ms to get response, and 1240 ms overall 17-03-18 00:08:13.218 -07 000000627 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 1203 ms to get response, and 1232 ms overall 17-03-18 00:08:13.258 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:13.268 -07 000000486 INF: FPGA: Recv YRDPOS 126082 17-03-18 00:08:13.268 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:08:13.268 -07 000000627 INF: Y Motor: Encoder = 126082 = motor step 98501.6 = 61.5635 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:13.268 -07 000000627 INF: Y Motor: Position error before settling (table move) = -19 encoder counts (-0.009 mm) 17-03-18 00:08:13.268 -07 000000627 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 1232 ms, distance = 0.840 mm, average velocity = 0.682 mm/sec 17-03-18 00:08:13.838 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:08:14.218 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:14.218 -07 000000482 INF: FPGA: Recv YRDPOS 126083 17-03-18 00:08:14.228 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:14.228 -07 000000627 INF: Y Motor: Encoder = 126083 = motor step 98502.3 = 61.5640 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:14.248 -07 000000627 INF: Y Motor: After move to 61.572609, error = -0.008644, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:14.248 -07 000000627 INF: Y Motor: Position error after settling (table move) = -18 encoder counts (-0.009 mm), Delta during settling = 1 counts (0.000 mm) 17-03-18 00:08:14.248 -07 000000627 INF: Y Motor: Moved to 61.5640 after 2274 ms (table move) 17-03-18 00:08:14.258 -07 000000476 INF: Z Motor: EndOfMoveAbsStepped: target = -0.177409 mm (DAC = 38755) 17-03-18 00:08:14.258 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.258 -07 000000478 INF: FPGA: Recv ZSGSRD 36912 17-03-18 00:08:14.268 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:14.268 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.268 -07 000000382 INF: FPGA: Recv ZSGSRD 36904 17-03-18 00:08:14.278 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.278 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.278 -07 000000478 INF: FPGA: Recv ZSGSRD 36905 17-03-18 00:08:14.288 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.288 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.288 -07 000000382 INF: FPGA: Recv ZSGSRD 36911 17-03-18 00:08:14.298 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.298 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.298 -07 000000608 INF: FPGA: Recv ZSGSRD 36899 17-03-18 00:08:14.308 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.308 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.308 -07 000000382 INF: FPGA: Recv ZSGSRD 36907 17-03-18 00:08:14.318 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.318 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.318 -07 000000627 INF: FPGA: Recv ZSGSRD 36907 17-03-18 00:08:14.328 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.328 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.328 -07 000000012 INF: FPGA: Recv ZSGSRD 36907 17-03-18 00:08:14.338 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.338 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.338 -07 000000586 INF: FPGA: Recv ZSGSRD 36909 17-03-18 00:08:14.348 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.348 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:14.348 -07 000000382 INF: FPGA: Recv ZSGSRD 36905 17-03-18 00:08:14.358 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.358 -07 000000476 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:14.358 -07 000000478 INF: FPGA: Recv ZPIEZORD 33019 17-03-18 00:08:14.368 -07 000000476 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:14.368 -07 000000476 INF: Z Motor: Piezo is near 50.5 V (nominally 0 to 100 V), expect near 59.1 V, since DAC is at 5.914 V. Piezo Error = -8.6 V. 17-03-18 00:08:14.368 -07 000000476 INF: Z Motor: ReadAdcAverage: Commanded Z = -177.4 um, Read 10 ADC samples: Avg = 36907 (-176.5 uncal um), Error = 0.9 uncal um, Median = 36907 (-176.5 uncal um), StdDev = 4 (0.0 uncal um), Range = 13 (0.1 uncal um), Min = 36899 (-176.5 uncal um), Max = 36912 (-176.6 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 8 (0.1 uncal um) 17-03-18 00:08:14.368 -07 000000476 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 36907, posNoise = 5, negNoise = 8 17-03-18 00:08:14.368 -07 000000476 INF: Z Motor: After move to -0.177409, error = -0.000013, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:14.368 -07 000000476 INF: Z Motor: Settle wait time = 2277 ms, measure time = 110 ms (allowed 150 ms), total time = 2387 ms 17-03-18 00:08:14.368 -07 000000476 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:14.408 -07 000000476 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 2431 ms 17-03-18 00:08:14.408 -07 000000476 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:14.408 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:14.408 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:14.408 -07 000000476 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:14.408 -07 000000382 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:08:14.408 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:14.408 -07 000000478 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:14.408 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:14.408 -07 000000382 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:08:14.418 -07 000000382 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:08:14.418 -07 000000382 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:08:14.428 -07 000000382 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 9 ms. 17-03-18 00:08:14.428 -07 000000382 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 13 ms. 17-03-18 00:08:14.428 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:08:14.468 -07 000000547 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:08:14.768 -07 000000547 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:08:14.768 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:08:14.768 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:08:14.778 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:08:14.808 -07 000000476 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:08:14.808 -07 000000627 INF: FPGA: Recv CAMSNAP 17-03-18 00:08:14.808 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 378 ms to get response, and 384 ms overall 17-03-18 00:08:14.898 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 127 ms. 17-03-18 00:08:14.898 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:14.908 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 2 ms. 17-03-18 00:08:14.908 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 131 ms. 17-03-18 00:08:15.008 -07 000000012 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:08:15.008 -07 000000627 INF: Autofocus start 17-03-18 00:08:15.008 -07 000000627 INF: MeasureProfile start 17-03-18 00:08:15.008 -07 000000476 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:08:15.018 -07 000000012 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:08:15.018 -07 000000012 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:08:15.018 -07 000000627 INF: MeasureProfile done 17-03-18 00:08:15.018 -07 000000627 INF: X=16 Z correction [um]=0.01042744 17-03-18 00:08:15.018 -07 000000627 INF: Autofocus finished 17-03-18 00:08:15.218 -07 000000608 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:08:15.218 -07 000000012 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 192 ms to get response, and 202 ms overall 17-03-18 00:08:15.218 -07 000000610 INF: ------------ Scan Snapping tile 3 at Y-position 61.56396484375, Z-position -0.177421749527107. ---------- 17-03-18 00:08:15.228 -07 000000610 INF: Cycle 82 Tile 3 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:15.228 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 37270, sync = True, waitForSettling = True) 17-03-18 00:08:15.228 -07 000000610 INF: Z Motor: Moving to -0.170611 mm, started at -0.177422 mm, distance = 6.81 um 17-03-18 00:08:15.228 -07 000000610 INF: FPGA: Xmit ZDACW 37270\n [Z Motor] 17-03-18 00:08:15.228 -07 000000627 INF: FPGA: Recv ZDACW 17-03-18 00:08:15.238 -07 000000610 INF: FPGA: 'ZDACW 37270' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:08:15.238 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:15.238 -07 000000482 INF: FPGA: Recv ZDACR 37270 17-03-18 00:08:15.248 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.248 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:15.398 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.398 -07 000000627 INF: FPGA: Recv ZSGSRD 35851 17-03-18 00:08:15.408 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:15.408 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.408 -07 000000627 INF: FPGA: Recv ZSGSRD 35848 17-03-18 00:08:15.418 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.418 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.418 -07 000000586 INF: FPGA: Recv ZSGSRD 35852 17-03-18 00:08:15.428 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.428 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.428 -07 000000627 INF: FPGA: Recv ZSGSRD 35847 17-03-18 00:08:15.438 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.438 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.438 -07 000000586 INF: FPGA: Recv ZSGSRD 35848 17-03-18 00:08:15.448 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.448 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.448 -07 000000627 INF: FPGA: Recv ZSGSRD 35852 17-03-18 00:08:15.458 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.458 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.458 -07 000000627 INF: FPGA: Recv ZSGSRD 35850 17-03-18 00:08:15.468 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.468 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.468 -07 000000382 INF: FPGA: Recv ZSGSRD 35849 17-03-18 00:08:15.478 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.478 -07 000000382 INF: FPGA: Recv ZSGSRD 35842 17-03-18 00:08:15.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:15.488 -07 000000382 INF: FPGA: Recv ZSGSRD 35849 17-03-18 00:08:15.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.498 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:15.498 -07 000000627 INF: FPGA: Recv ZPIEZORD 31598 17-03-18 00:08:15.508 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:15.508 -07 000000610 INF: Z Motor: Piezo is near 47.5 V (nominally 0 to 100 V), expect near 56.9 V, since DAC is at 5.687 V. Piezo Error = -9.4 V. 17-03-18 00:08:15.508 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -170.6 um, Read 10 ADC samples: Avg = 35849 (-169.7 uncal um), Error = 0.9 uncal um, Median = 35849 (-169.7 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 35842 (-169.7 uncal um), Max = 35852 (-169.8 uncal um), Pos Noise = 3 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:08:15.508 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35849, posNoise = 3, negNoise = 7 17-03-18 00:08:15.508 -07 000000610 INF: Z Motor: After move to -0.170611, error = -0.000014, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:15.508 -07 000000610 INF: Z Motor: MoveAbsStepped took 278 ms) 17-03-18 00:08:15.508 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:15.508 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:15.508 -07 000000482 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:15.508 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:15.508 -07 000000586 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:15.508 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:15.508 -07 000000547 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:15.508 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:15.508 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:15.508 -07 000000586 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:15.508 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:08:15.508 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:15.518 -07 000000586 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:15.518 -07 000000586 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:15.518 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:08:15.518 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:15.528 -07 000000586 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:08:15.528 -07 000000586 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:08:15.528 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:08:15.528 -07 000000547 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:08:15.528 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 37447 150\n [Camera] 17-03-18 00:08:15.728 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:16.768 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:16.768 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:16.768 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:16.768 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:16.768 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:16.778 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:16.778 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:16.778 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:08:16.778 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:16.778 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:08:16.958 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 37447 1422 17-03-18 00:08:17.108 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:17.138 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:17.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:17.168 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 391 ms. 17-03-18 00:08:17.168 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 391 ms. 17-03-18 00:08:17.168 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:17.168 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 392 ms. 17-03-18 00:08:17.168 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 392 ms. 17-03-18 00:08:17.168 -07 000000586 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:17.168 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:17.168 -07 000000586 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:17.178 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:08:17.178 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 402 ms. 17-03-18 00:08:17.608 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:17.618 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:17.618 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:17.618 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:17.618 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) start. 17-03-18 00:08:17.618 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:17.618 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:17.618 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:17.618 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:17.618 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 1130172064, 0x0, 418435064) start. 17-03-18 00:08:17.808 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP 2266 17-03-18 00:08:17.808 -07 000000547 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:17.808 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 37447 150' command took 2 ms to send, 2263 ms to get response, and 2274 ms overall 17-03-18 00:08:17.808 -07 000000610 INF: Skipping AutoFocus in Tile 4: adopting Best-Z from the cache: -0.170864 17-03-18 00:08:17.808 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 62.4026 mm, moving Z to -0.17086 mm 17-03-18 00:08:17.808 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:17.808 -07 000000382 INF: Y Motor: Entering MoveAbsStepped (target = 99844 steps, sync = True, waitForSettling = True) 17-03-18 00:08:17.808 -07 000000382 INF: Y Motor: Moving to 62.4026 mm, started at 61.5640 mm, delta = 0.8386 mm 17-03-18 00:08:17.808 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:17.808 -07 000000012 INF: Z Motor: Entering MoveAbsStepped (DAC = 37325, sync = True, waitForSettling = False) 17-03-18 00:08:17.808 -07 000000012 INF: Z Motor: Moving to -0.170863 mm, started at -0.170625 mm, distance = -0.24 um 17-03-18 00:08:17.928 -07 000000012 INF: FPGA: Xmit ZDACW 37325\n [Z Motor] 17-03-18 00:08:17.928 -07 000000586 INF: FPGA: Recv YRDPOS 126083 17-03-18 00:08:17.928 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 117 ms overall 17-03-18 00:08:17.928 -07 000000382 INF: Y Motor: Encoder = 126083 = motor step 98502.3 = 61.5640 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:17.928 -07 000000382 INF: Y Motor: Last commanded position = 61.5726 mm (98516.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:17.928 -07 000000382 INF: Y Motor: Moving 1342 steps-19 17-03-18 00:08:17.928 -07 000000547 INF: FPGA: Recv ZDACW 17-03-18 00:08:17.938 -07 000000012 INF: FPGA: 'ZDACW 37325' command took 0 ms to send, 0 ms to get response, and 121 ms overall 17-03-18 00:08:17.938 -07 000000012 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:17.938 -07 000000448 INF: FPGA: Recv ZDACR 37325 17-03-18 00:08:17.948 -07 000000382 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:17.948 -07 000000012 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:17.948 -07 000000012 INF: Z Motor: MoveAbsStepped took 132 ms (did NOT wait for settling yet)) 17-03-18 00:08:18.008 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 1130172064, 0x0, 418435064) end. Duration was 387 ms. 17-03-18 00:08:18.008 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 388 ms. 17-03-18 00:08:18.008 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:18.008 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) end. Duration was 392 ms. 17-03-18 00:08:18.008 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 392 ms. 17-03-18 00:08:18.008 -07 000000476 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:18.008 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:18.018 -07 000000476 INF: Camera: CopyActivePixFullImage end. Duration was 6 ms. 17-03-18 00:08:18.018 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:18.018 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 398 ms. 17-03-18 00:08:18.318 -07 000000012 INF: FPGA: Recv YMVTILE 17-03-18 00:08:18.318 -07 000000382 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 391 ms overall 17-03-18 00:08:18.368 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:18.368 -07 000000476 INF: FPGA: Recv YRDPOS 127786 17-03-18 00:08:18.378 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:08:18.378 -07 000000382 INF: Y Motor: Encoder = 127786 = motor step 99832.8 = 62.3955 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:18.378 -07 000000382 INF: Y Motor: Position error before settling (table move) = -15 encoder counts (-0.007 mm) 17-03-18 00:08:18.378 -07 000000382 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 392 ms, distance = 0.839 mm, average velocity = 2.139 mm/sec 17-03-18 00:08:19.208 -07 000000627 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:08:19.328 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:19.328 -07 000000547 INF: FPGA: Recv YRDPOS 127786 17-03-18 00:08:19.338 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.338 -07 000000382 INF: Y Motor: Encoder = 127786 = motor step 99832.8 = 62.3955 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:19.358 -07 000000382 INF: Y Motor: After move to 62.402607, error = -0.007099, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:19.358 -07 000000382 INF: Y Motor: Position error after settling (table move) = -15 encoder counts (-0.007 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:19.358 -07 000000382 INF: Y Motor: Moved to 62.3955 after 1549 ms (table move) 17-03-18 00:08:19.358 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.170863 mm (DAC = 37325) 17-03-18 00:08:19.358 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.368 -07 000000382 INF: FPGA: Recv ZSGSRD 35892 17-03-18 00:08:19.378 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:19.378 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.378 -07 000000586 INF: FPGA: Recv ZSGSRD 35891 17-03-18 00:08:19.388 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.388 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.388 -07 000000012 INF: FPGA: Recv ZSGSRD 35887 17-03-18 00:08:19.398 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.398 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.398 -07 000000382 INF: FPGA: Recv ZSGSRD 35893 17-03-18 00:08:19.408 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.408 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.408 -07 000000012 INF: FPGA: Recv ZSGSRD 35893 17-03-18 00:08:19.418 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.418 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.418 -07 000000586 INF: FPGA: Recv ZSGSRD 35888 17-03-18 00:08:19.428 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.428 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.428 -07 000000476 INF: FPGA: Recv ZSGSRD 35888 17-03-18 00:08:19.438 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.438 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.438 -07 000000586 INF: FPGA: Recv ZSGSRD 35882 17-03-18 00:08:19.448 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.448 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.448 -07 000000476 INF: FPGA: Recv ZSGSRD 35885 17-03-18 00:08:19.458 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.458 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.458 -07 000000382 INF: FPGA: Recv ZSGSRD 35889 17-03-18 00:08:19.468 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.468 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:19.468 -07 000000476 INF: FPGA: Recv ZPIEZORD 31678 17-03-18 00:08:19.478 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.478 -07 000000610 INF: Z Motor: Piezo is near 47.7 V (nominally 0 to 100 V), expect near 57.0 V, since DAC is at 5.695 V. Piezo Error = -9.3 V. 17-03-18 00:08:19.478 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -170.9 um, Read 10 ADC samples: Avg = 35889 (-170.0 uncal um), Error = 0.9 uncal um, Median = 35888 (-170.0 uncal um), StdDev = 4 (0.0 uncal um), Range = 11 (0.1 uncal um), Min = 35882 (-170.0 uncal um), Max = 35893 (-170.0 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:08:19.478 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35889, posNoise = 4, negNoise = 7 17-03-18 00:08:19.478 -07 000000610 INF: Z Motor: After move to -0.170863, error = -0.000019, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:19.478 -07 000000610 INF: Z Motor: Settle wait time = 1551 ms, measure time = 111 ms (allowed 150 ms), total time = 1662 ms 17-03-18 00:08:19.478 -07 000000610 INF: Z Motor: Waiting extra 39 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:19.518 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1704 ms 17-03-18 00:08:19.518 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:19.518 -07 000000610 INF: ------------ Scan Snapping tile 4 at Y-position 62.3955078125, Z-position -0.170881901566794. ---------- 17-03-18 00:08:19.518 -07 000000610 INF: Cycle 82 Tile 4 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:19.518 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 37149, sync = True, waitForSettling = True) 17-03-18 00:08:19.518 -07 000000610 INF: Z Motor: Moving to -0.170057 mm, started at -0.170882 mm, distance = 0.82 um 17-03-18 00:08:19.518 -07 000000610 INF: FPGA: Xmit ZDACW 37149\n [Z Motor] 17-03-18 00:08:19.518 -07 000000486 INF: FPGA: Recv ZDACW 17-03-18 00:08:19.528 -07 000000610 INF: FPGA: 'ZDACW 37149' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:08:19.528 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:19.528 -07 000000627 INF: FPGA: Recv ZDACR 37149 17-03-18 00:08:19.538 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.538 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:19.688 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.688 -07 000000512 INF: FPGA: Recv ZSGSRD 35768 17-03-18 00:08:19.698 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:19.698 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.698 -07 000000627 INF: FPGA: Recv ZSGSRD 35769 17-03-18 00:08:19.708 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.708 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.708 -07 000000486 INF: FPGA: Recv ZSGSRD 35764 17-03-18 00:08:19.718 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.718 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.718 -07 000000547 INF: FPGA: Recv ZSGSRD 35758 17-03-18 00:08:19.728 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.728 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.728 -07 000000512 INF: FPGA: Recv ZSGSRD 35764 17-03-18 00:08:19.738 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.738 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.738 -07 000000547 INF: FPGA: Recv ZSGSRD 35769 17-03-18 00:08:19.748 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:19.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.748 -07 000000627 INF: FPGA: Recv ZSGSRD 35758 17-03-18 00:08:19.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.758 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.758 -07 000000486 INF: FPGA: Recv ZSGSRD 35760 17-03-18 00:08:19.768 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.768 -07 000000512 INF: FPGA: Recv ZSGSRD 35762 17-03-18 00:08:19.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:19.778 -07 000000512 INF: FPGA: Recv ZSGSRD 35765 17-03-18 00:08:19.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:19.788 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:19.788 -07 000000486 INF: FPGA: Recv ZPIEZORD 31519 17-03-18 00:08:19.798 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:19.798 -07 000000610 INF: Z Motor: Piezo is near 47.3 V (nominally 0 to 100 V), expect near 56.7 V, since DAC is at 5.669 V. Piezo Error = -9.4 V. 17-03-18 00:08:19.798 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -170.1 um, Read 10 ADC samples: Avg = 35764 (-169.2 uncal um), Error = 0.9 uncal um, Median = 35764 (-169.2 uncal um), StdDev = 4 (0.0 uncal um), Range = 11 (0.1 uncal um), Min = 35758 (-169.2 uncal um), Max = 35769 (-169.2 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:08:19.798 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35764, posNoise = 5, negNoise = 6 17-03-18 00:08:19.798 -07 000000610 INF: Z Motor: After move to -0.170057, error = -0.000022, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:19.798 -07 000000610 INF: Z Motor: MoveAbsStepped took 276 ms) 17-03-18 00:08:19.798 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:19.798 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:19.798 -07 000000482 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:19.798 -07 000000486 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:19.798 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:19.798 -07 000000382 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:19.798 -07 000000547 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:19.798 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:19.798 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:08:19.798 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:19.798 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:19.798 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:19.798 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:08:19.798 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:19.808 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:19.808 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:19.818 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 13 ms. 17-03-18 00:08:19.818 -07 000000486 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 18 ms. 17-03-18 00:08:19.818 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 13 ms. 17-03-18 00:08:19.818 -07 000000547 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 19 ms. 17-03-18 00:08:19.818 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 37325 150\n [Camera] 17-03-18 00:08:20.018 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:21.058 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:21.058 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:21.058 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:21.058 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:21.058 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:21.068 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:21.068 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:21.068 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:08:21.068 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:21.068 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:08:21.248 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 37325 1422 17-03-18 00:08:21.398 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:21.458 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 393 ms. 17-03-18 00:08:21.458 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:08:21.458 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:21.458 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:21.458 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:08:21.468 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 395 ms. 17-03-18 00:08:21.468 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 396 ms. 17-03-18 00:08:21.468 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:21.468 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:21.478 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 414 ms. 17-03-18 00:08:21.898 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:21.898 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:21.908 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:21.908 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:21.908 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:21.908 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:21.908 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:21.908 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:08:21.908 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:21.908 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) start. 17-03-18 00:08:22.098 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:22.098 -07 000000478 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:22.098 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 37325 150' command took 2 ms to send, 2261 ms to get response, and 2272 ms overall 17-03-18 00:08:22.098 -07 000000610 INF: Skipping AutoFocus in Tile 5: adopting Best-Z from the cache: -0.170564 17-03-18 00:08:22.098 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 63.2326 mm, moving Z to -0.17056 mm 17-03-18 00:08:22.098 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:22.098 -07 000000486 INF: Y Motor: Entering MoveAbsStepped (target = 101172 steps, sync = True, waitForSettling = True) 17-03-18 00:08:22.098 -07 000000486 INF: Y Motor: Moving to 63.2326 mm, started at 62.3955 mm, delta = 0.8371 mm 17-03-18 00:08:22.098 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:22.098 -07 000000512 INF: Z Motor: Entering MoveAbsStepped (DAC = 37260, sync = True, waitForSettling = False) 17-03-18 00:08:22.098 -07 000000512 INF: Z Motor: Moving to -0.170565 mm, started at -0.170079 mm, distance = -0.49 um 17-03-18 00:08:22.108 -07 000000512 INF: FPGA: Xmit ZDACW 37260\n [Z Motor] 17-03-18 00:08:22.108 -07 000000482 INF: FPGA: Recv YRDPOS 127786 17-03-18 00:08:22.108 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 3 ms to get response, and 6 ms overall 17-03-18 00:08:22.108 -07 000000486 INF: Y Motor: Encoder = 127786 = motor step 99832.8 = 62.3955 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:22.108 -07 000000486 INF: Y Motor: Last commanded position = 62.4026 mm (99844.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:22.108 -07 000000486 INF: Y Motor: Moving 1339 steps-15 17-03-18 00:08:22.108 -07 000000478 INF: FPGA: Recv ZDACW 17-03-18 00:08:22.118 -07 000000512 INF: FPGA: 'ZDACW 37260' command took 0 ms to send, 0 ms to get response, and 12 ms overall 17-03-18 00:08:22.118 -07 000000512 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:22.118 -07 000000478 INF: FPGA: Recv ZDACR 37260 17-03-18 00:08:22.128 -07 000000486 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:22.128 -07 000000512 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:22.128 -07 000000512 INF: Z Motor: MoveAbsStepped took 23 ms (did NOT wait for settling yet)) 17-03-18 00:08:22.138 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:22.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:22.308 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) end. Duration was 398 ms. 17-03-18 00:08:22.308 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:22.308 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:22.308 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 402 ms. 17-03-18 00:08:22.308 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 402 ms. 17-03-18 00:08:22.308 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:22.318 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:22.318 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 410 ms. 17-03-18 00:08:22.318 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:22.318 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 413 ms. 17-03-18 00:08:22.508 -07 000000608 INF: FPGA: Recv YMVTILE 17-03-18 00:08:22.508 -07 000000486 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 372 ms to get response, and 399 ms overall 17-03-18 00:08:22.548 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:22.548 -07 000000382 INF: FPGA: Recv YRDPOS 129482 17-03-18 00:08:22.558 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:08:22.558 -07 000000486 INF: Y Motor: Encoder = 129482 = motor step 101157.8 = 63.2236 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:22.558 -07 000000486 INF: Y Motor: Position error before settling (table move) = -18 encoder counts (-0.009 mm) 17-03-18 00:08:22.558 -07 000000486 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 399 ms, distance = 0.837 mm, average velocity = 2.098 mm/sec 17-03-18 00:08:23.508 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:23.508 -07 000000547 INF: FPGA: Recv YRDPOS 129482 17-03-18 00:08:23.518 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:23.518 -07 000000486 INF: Y Motor: Encoder = 129482 = motor step 101157.8 = 63.2236 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:23.538 -07 000000486 INF: Y Motor: After move to 63.232609, error = -0.008976, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:23.538 -07 000000486 INF: Y Motor: Position error after settling (table move) = -18 encoder counts (-0.009 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:23.538 -07 000000486 INF: Y Motor: Moved to 63.2236 after 1439 ms (table move) 17-03-18 00:08:23.548 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.170565 mm (DAC = 37260) 17-03-18 00:08:23.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.548 -07 000000382 INF: FPGA: Recv ZSGSRD 35838 17-03-18 00:08:23.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.558 -07 000000547 INF: FPGA: Recv ZSGSRD 35842 17-03-18 00:08:23.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.568 -07 000000382 INF: FPGA: Recv ZSGSRD 35843 17-03-18 00:08:23.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.578 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.578 -07 000000486 INF: FPGA: Recv ZSGSRD 35841 17-03-18 00:08:23.588 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.588 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.588 -07 000000382 INF: FPGA: Recv ZSGSRD 35842 17-03-18 00:08:23.598 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.598 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.598 -07 000000486 INF: FPGA: Recv ZSGSRD 35835 17-03-18 00:08:23.608 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.608 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.608 -07 000000382 INF: FPGA: Recv ZSGSRD 35837 17-03-18 00:08:23.618 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.618 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.618 -07 000000486 INF: FPGA: Recv ZSGSRD 35846 17-03-18 00:08:23.628 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.628 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.628 -07 000000382 INF: FPGA: Recv ZSGSRD 35834 17-03-18 00:08:23.638 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.638 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.638 -07 000000547 INF: FPGA: Recv ZSGSRD 35844 17-03-18 00:08:23.648 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.648 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:23.648 -07 000000482 INF: FPGA: Recv ZPIEZORD 31645 17-03-18 00:08:23.658 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.658 -07 000000610 INF: Z Motor: Piezo is near 47.6 V (nominally 0 to 100 V), expect near 56.9 V, since DAC is at 5.686 V. Piezo Error = -9.3 V. 17-03-18 00:08:23.658 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -170.6 um, Read 10 ADC samples: Avg = 35840 (-169.7 uncal um), Error = 0.9 uncal um, Median = 35842 (-169.7 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 35834 (-169.7 uncal um), Max = 35846 (-169.7 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:08:23.658 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35840, posNoise = 6, negNoise = 6 17-03-18 00:08:23.658 -07 000000610 INF: Z Motor: After move to -0.170565, error = -0.000002, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:23.658 -07 000000610 INF: Z Motor: Settle wait time = 1443 ms, measure time = 110 ms (allowed 150 ms), total time = 1553 ms 17-03-18 00:08:23.658 -07 000000610 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:23.698 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1594 ms 17-03-18 00:08:23.698 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:23.698 -07 000000610 INF: ------------ Scan Snapping tile 5 at Y-position 63.2236328125, Z-position -0.170567115171848. ---------- 17-03-18 00:08:23.698 -07 000000610 INF: Cycle 82 Tile 5 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:23.698 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 37083, sync = True, waitForSettling = True) 17-03-18 00:08:23.698 -07 000000610 INF: Z Motor: Moving to -0.169755 mm, started at -0.170567 mm, distance = 0.81 um 17-03-18 00:08:23.698 -07 000000610 INF: FPGA: Xmit ZDACW 37083\n [Z Motor] 17-03-18 00:08:23.708 -07 000000608 INF: FPGA: Recv ZDACW 17-03-18 00:08:23.708 -07 000000610 INF: FPGA: 'ZDACW 37083' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:08:23.708 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:23.708 -07 000000482 INF: FPGA: Recv ZDACR 37083 17-03-18 00:08:23.718 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:23.718 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:23.868 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.868 -07 000000482 INF: FPGA: Recv ZSGSRD 35718 17-03-18 00:08:23.878 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:23.878 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.878 -07 000000486 INF: FPGA: Recv ZSGSRD 35715 17-03-18 00:08:23.888 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.888 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.888 -07 000000482 INF: FPGA: Recv ZSGSRD 35716 17-03-18 00:08:23.898 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.898 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.898 -07 000000486 INF: FPGA: Recv ZSGSRD 35713 17-03-18 00:08:23.908 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.908 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.908 -07 000000486 INF: FPGA: Recv ZSGSRD 35711 17-03-18 00:08:23.918 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.918 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.918 -07 000000486 INF: FPGA: Recv ZSGSRD 35703 17-03-18 00:08:23.928 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.928 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.928 -07 000000486 INF: FPGA: Recv ZSGSRD 35715 17-03-18 00:08:23.938 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.948 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.948 -07 000000478 INF: FPGA: Recv ZSGSRD 35718 17-03-18 00:08:23.958 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:23.958 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.958 -07 000000012 INF: FPGA: Recv ZSGSRD 35712 17-03-18 00:08:23.968 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.968 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:23.968 -07 000000012 INF: FPGA: Recv ZSGSRD 35715 17-03-18 00:08:23.978 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.978 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:23.978 -07 000000482 INF: FPGA: Recv ZPIEZORD 31449 17-03-18 00:08:23.988 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:23.988 -07 000000610 INF: Z Motor: Piezo is near 47.2 V (nominally 0 to 100 V), expect near 56.6 V, since DAC is at 5.659 V. Piezo Error = -9.4 V. 17-03-18 00:08:23.988 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -169.8 um, Read 10 ADC samples: Avg = 35714 (-168.9 uncal um), Error = 0.9 uncal um, Median = 35715 (-168.9 uncal um), StdDev = 4 (0.0 uncal um), Range = 15 (0.1 uncal um), Min = 35703 (-168.8 uncal um), Max = 35718 (-168.9 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 11 (0.1 uncal um) 17-03-18 00:08:23.988 -07 000000610 INF: Z Motor: ADC noise = 11, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35714, posNoise = 4, negNoise = 11 17-03-18 00:08:23.988 -07 000000610 INF: Z Motor: After move to -0.169755, error = -0.000003, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:23.988 -07 000000610 INF: Z Motor: MoveAbsStepped took 284 ms) 17-03-18 00:08:23.988 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:23.988 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:23.988 -07 000000476 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:23.988 -07 000000547 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:23.988 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:23.988 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:23.988 -07 000000608 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:23.988 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:23.988 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:08:23.988 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 2 ms. 17-03-18 00:08:23.988 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:23.988 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:23.998 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:23.998 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:23.998 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:08:23.998 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:24.008 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 8 ms. 17-03-18 00:08:24.008 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 15 ms. 17-03-18 00:08:24.008 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 9 ms. 17-03-18 00:08:24.008 -07 000000608 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 16 ms. 17-03-18 00:08:24.008 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 37260 150\n [Camera] 17-03-18 00:08:24.208 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning red on 195 17-03-18 00:08:24.588 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:08:25.248 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:25.248 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:25.248 -07 000000482 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:25.248 -07 000000482 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:25.248 -07 000000482 INF: Camera: sBCa_ReadImage(785074288, 1165594328, 0x0, 391868352) start. 17-03-18 00:08:25.248 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:25.248 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:25.248 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:25.248 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:25.248 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2147487792, 0x0, 418435064) start. 17-03-18 00:08:25.438 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 37260 1422 17-03-18 00:08:25.588 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:25.648 -07 000000482 INF: Camera: sBCa_ReadImage(785074288, 1165594328, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:08:25.648 -07 000000482 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:08:25.648 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:25.648 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2147487792, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:08:25.648 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:25.648 -07 000000482 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:08:25.648 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:08:25.648 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:25.648 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:25.658 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:08:26.088 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:26.088 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:26.088 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:26.088 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:26.088 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2182910056, 0x0, 391868352) start. 17-03-18 00:08:26.088 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:26.088 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:26.088 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:26.088 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:26.088 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 2218332320, 0x0, 418435064) start. 17-03-18 00:08:26.278 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:26.278 -07 000000012 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:26.278 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 37260 150' command took 2 ms to send, 2265 ms to get response, and 2272 ms overall 17-03-18 00:08:26.278 -07 000000610 INF: Skipping AutoFocus in Tile 6: adopting Best-Z from the cache: -0.170305 17-03-18 00:08:26.278 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 64.0626 mm, moving Z to -0.17030 mm 17-03-18 00:08:26.278 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:26.278 -07 000000478 INF: Y Motor: Entering MoveAbsStepped (target = 102500 steps, sync = True, waitForSettling = True) 17-03-18 00:08:26.278 -07 000000478 INF: Y Motor: Moving to 64.0626 mm, started at 63.2236 mm, delta = 0.8390 mm 17-03-18 00:08:26.278 -07 000000478 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:26.288 -07 000000476 INF: Z Motor: Entering MoveAbsStepped (DAC = 37203, sync = True, waitForSettling = False) 17-03-18 00:08:26.288 -07 000000476 INF: Z Motor: Moving to -0.170304 mm, started at -0.169758 mm, distance = -0.55 um 17-03-18 00:08:26.288 -07 000000382 INF: FPGA: Recv YRDPOS 129483 17-03-18 00:08:26.298 -07 000000478 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:26.298 -07 000000476 INF: FPGA: Xmit ZDACW 37203\n [Z Motor] 17-03-18 00:08:26.298 -07 000000478 INF: Y Motor: Encoder = 129483 = motor step 101158.6 = 63.2241 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:26.298 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:08:26.298 -07 000000478 INF: Y Motor: Last commanded position = 63.2326 mm (101172.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:26.298 -07 000000478 INF: Y Motor: Moving 1342 steps-18 17-03-18 00:08:26.308 -07 000000478 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:26.308 -07 000000476 INF: FPGA: 'ZDACW 37203' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:26.318 -07 000000476 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:26.318 -07 000000012 INF: FPGA: Recv ZDACR 37203 17-03-18 00:08:26.328 -07 000000476 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:26.328 -07 000000476 INF: Z Motor: MoveAbsStepped took 40 ms (did NOT wait for settling yet)) 17-03-18 00:08:26.478 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 2218332320, 0x0, 418435064) end. Duration was 389 ms. 17-03-18 00:08:26.478 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 389 ms. 17-03-18 00:08:26.478 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:26.478 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2182910056, 0x0, 391868352) end. Duration was 394 ms. 17-03-18 00:08:26.478 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 395 ms. 17-03-18 00:08:26.478 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:26.488 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:26.488 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:26.488 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:26.488 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:08:26.678 -07 000000512 INF: FPGA: Recv YMVTILE 17-03-18 00:08:26.678 -07 000000478 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 369 ms to get response, and 385 ms overall 17-03-18 00:08:26.728 -07 000000478 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:26.728 -07 000000547 INF: FPGA: Recv YRDPOS 131189 17-03-18 00:08:26.738 -07 000000478 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:26.738 -07 000000478 INF: Y Motor: Encoder = 131189 = motor step 102491.4 = 64.0571 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:26.738 -07 000000478 INF: Y Motor: Position error before settling (table move) = -11 encoder counts (-0.005 mm) 17-03-18 00:08:26.738 -07 000000478 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 386 ms, distance = 0.839 mm, average velocity = 2.174 mm/sec 17-03-18 00:08:27.138 -07 000000608 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:27.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:27.688 -07 000000478 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:27.688 -07 000000512 INF: FPGA: Recv YRDPOS 131189 17-03-18 00:08:27.698 -07 000000478 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:27.698 -07 000000478 INF: Y Motor: Encoder = 131189 = motor step 102491.4 = 64.0571 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:27.718 -07 000000478 INF: Y Motor: After move to 64.062607, error = -0.005478, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:27.718 -07 000000478 INF: Y Motor: Position error after settling (table move) = -11 encoder counts (-0.005 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:27.718 -07 000000478 INF: Y Motor: Moved to 64.0571 after 1434 ms (table move) 17-03-18 00:08:27.718 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.170304 mm (DAC = 37203) 17-03-18 00:08:27.718 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.718 -07 000000382 INF: FPGA: Recv ZSGSRD 35794 17-03-18 00:08:27.728 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:08:27.728 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.728 -07 000000382 INF: FPGA: Recv ZSGSRD 35793 17-03-18 00:08:27.738 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.738 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.738 -07 000000382 INF: FPGA: Recv ZSGSRD 35801 17-03-18 00:08:27.748 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.748 -07 000000382 INF: FPGA: Recv ZSGSRD 35804 17-03-18 00:08:27.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.758 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.758 -07 000000482 INF: FPGA: Recv ZSGSRD 35799 17-03-18 00:08:27.768 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.768 -07 000000476 INF: FPGA: Recv ZSGSRD 35798 17-03-18 00:08:27.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.778 -07 000000448 INF: FPGA: Recv ZSGSRD 35799 17-03-18 00:08:27.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.788 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.788 -07 000000476 INF: FPGA: Recv ZSGSRD 35797 17-03-18 00:08:27.798 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.798 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.798 -07 000000482 INF: FPGA: Recv ZSGSRD 35800 17-03-18 00:08:27.808 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.808 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:27.808 -07 000000476 INF: FPGA: Recv ZSGSRD 35796 17-03-18 00:08:27.818 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.818 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:27.818 -07 000000382 INF: FPGA: Recv ZPIEZORD 31618 17-03-18 00:08:27.828 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.828 -07 000000610 INF: Z Motor: Piezo is near 47.5 V (nominally 0 to 100 V), expect near 56.8 V, since DAC is at 5.677 V. Piezo Error = -9.2 V. 17-03-18 00:08:27.828 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -170.3 um, Read 10 ADC samples: Avg = 35798 (-169.4 uncal um), Error = 0.9 uncal um, Median = 35798 (-169.4 uncal um), StdDev = 3 (0.0 uncal um), Range = 11 (0.1 uncal um), Min = 35793 (-169.4 uncal um), Max = 35804 (-169.5 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:08:27.828 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35798, posNoise = 6, negNoise = 5 17-03-18 00:08:27.828 -07 000000610 INF: Z Motor: After move to -0.170304, error = 0.000007, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:27.828 -07 000000610 INF: Z Motor: Settle wait time = 1436 ms, measure time = 104 ms (allowed 150 ms), total time = 1540 ms 17-03-18 00:08:27.828 -07 000000610 INF: Z Motor: Waiting extra 46 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:27.878 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1592 ms 17-03-18 00:08:27.878 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:27.878 -07 000000610 INF: ------------ Scan Snapping tile 6 at Y-position 64.05712890625, Z-position -0.170297298261894. ---------- 17-03-18 00:08:27.878 -07 000000610 INF: Cycle 82 Tile 6 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:27.878 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 37026, sync = True, waitForSettling = True) 17-03-18 00:08:27.878 -07 000000610 INF: Z Motor: Moving to -0.169494 mm, started at -0.170297 mm, distance = 0.80 um 17-03-18 00:08:27.878 -07 000000610 INF: FPGA: Xmit ZDACW 37026\n [Z Motor] 17-03-18 00:08:27.878 -07 000000478 INF: FPGA: Recv ZDACW 17-03-18 00:08:27.888 -07 000000610 INF: FPGA: 'ZDACW 37026' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:08:27.888 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:27.888 -07 000000448 INF: FPGA: Recv ZDACR 37026 17-03-18 00:08:27.898 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:27.898 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:28.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.048 -07 000000476 INF: FPGA: Recv ZSGSRD 35676 17-03-18 00:08:28.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:28.058 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.058 -07 000000448 INF: FPGA: Recv ZSGSRD 35678 17-03-18 00:08:28.068 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.068 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.068 -07 000000512 INF: FPGA: Recv ZSGSRD 35677 17-03-18 00:08:28.078 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.078 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.078 -07 000000448 INF: FPGA: Recv ZSGSRD 35672 17-03-18 00:08:28.088 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.088 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.088 -07 000000512 INF: FPGA: Recv ZSGSRD 35672 17-03-18 00:08:28.098 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.098 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.098 -07 000000476 INF: FPGA: Recv ZSGSRD 35677 17-03-18 00:08:28.108 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.108 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.108 -07 000000382 INF: FPGA: Recv ZSGSRD 35672 17-03-18 00:08:28.118 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.118 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.118 -07 000000608 INF: FPGA: Recv ZSGSRD 35676 17-03-18 00:08:28.128 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.128 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.128 -07 000000448 INF: FPGA: Recv ZSGSRD 35673 17-03-18 00:08:28.138 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.138 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:28.138 -07 000000478 INF: FPGA: Recv ZSGSRD 35676 17-03-18 00:08:28.148 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.148 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:28.148 -07 000000608 INF: FPGA: Recv ZPIEZORD 31421 17-03-18 00:08:28.158 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:28.158 -07 000000610 INF: Z Motor: Piezo is near 47.1 V (nominally 0 to 100 V), expect near 56.5 V, since DAC is at 5.650 V. Piezo Error = -9.4 V. 17-03-18 00:08:28.158 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -169.5 um, Read 10 ADC samples: Avg = 35675 (-168.6 uncal um), Error = 0.9 uncal um, Median = 35676 (-168.6 uncal um), StdDev = 2 (0.0 uncal um), Range = 6 (0.0 uncal um), Min = 35672 (-168.6 uncal um), Max = 35678 (-168.7 uncal um), Pos Noise = 3 (0.0 uncal um), Neg Noise = 3 (0.0 uncal um) 17-03-18 00:08:28.158 -07 000000610 INF: Z Motor: ADC noise = 3, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35675, posNoise = 3, negNoise = 3 17-03-18 00:08:28.158 -07 000000610 INF: Z Motor: After move to -0.169494, error = -0.000013, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:28.158 -07 000000610 INF: Z Motor: MoveAbsStepped took 275 ms) 17-03-18 00:08:28.158 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:28.158 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:28.158 -07 000000512 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:28.158 -07 000000482 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:28.158 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:28.158 -07 000000382 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:28.158 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:28.158 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:28.168 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 3 ms. 17-03-18 00:08:28.168 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:28.168 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:28.168 -07 000000382 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:28.168 -07 000000382 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:28.168 -07 000000382 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:28.168 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:08:28.168 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:28.178 -07 000000382 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 8 ms. 17-03-18 00:08:28.178 -07 000000382 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 16 ms. 17-03-18 00:08:28.178 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 9 ms. 17-03-18 00:08:28.178 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 18 ms. 17-03-18 00:08:28.178 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 37203 150\n [Camera] 17-03-18 00:08:28.378 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:29.418 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:29.418 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:29.418 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:29.418 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:29.418 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2281705520, 0x0, 391868352) start. 17-03-18 00:08:29.418 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:29.418 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:29.418 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:29.418 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:29.418 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 2317127784, 0x0, 418435064) start. 17-03-18 00:08:29.608 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 37203 1422 17-03-18 00:08:29.758 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:29.818 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 2317127784, 0x0, 418435064) end. Duration was 393 ms. 17-03-18 00:08:29.938 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 393 ms. 17-03-18 00:08:29.938 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:29.938 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2281705520, 0x0, 391868352) end. Duration was 515 ms. 17-03-18 00:08:29.938 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 516 ms. 17-03-18 00:08:29.938 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:29.938 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 5 ms. 17-03-18 00:08:29.938 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:08:29.938 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 521 ms. 17-03-18 00:08:29.948 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:08:29.948 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 526 ms. 17-03-18 00:08:30.258 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:30.258 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:30.258 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:30.268 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:30.268 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:30.268 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:30.268 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:30.268 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:08:30.268 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:30.268 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) start. 17-03-18 00:08:30.448 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP 2264 17-03-18 00:08:30.448 -07 000000512 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:30.448 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 37203 150' command took 2 ms to send, 2269 ms to get response, and 2272 ms overall 17-03-18 00:08:30.458 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 0 - Best Z for this model is: -0.172880 17-03-18 00:08:30.458 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 7 at Y-position 64.05712890625, Z-Position -0.169507120168457. ------ 17-03-18 00:08:30.458 -07 000000476 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:08:30.458 -07 000000586 INF: Y Motor: MoveYZ optimized starting. Moving Y to 64.8926 mm, moving Z to -0.17596 mm 17-03-18 00:08:30.458 -07 000000586 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:30.468 -07 000000478 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:08:30.468 -07 000000476 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 1 ms to get response, and 3 ms overall 17-03-18 00:08:30.468 -07 000000476 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:08:30.468 -07 000000512 INF: Y Motor: Entering MoveAbsStepped (target = 103828 steps, sync = True, waitForSettling = True) 17-03-18 00:08:30.468 -07 000000512 INF: Y Motor: Moving to 64.8926 mm, started at 64.0571 mm, delta = 0.8355 mm 17-03-18 00:08:30.468 -07 000000448 INF: Z Motor: Entering MoveAbsStepped (DAC = 38438, sync = True, waitForSettling = False) 17-03-18 00:08:30.468 -07 000000448 INF: Z Motor: Moving to -0.175958 mm, started at -0.169507 mm, distance = -6.45 um 17-03-18 00:08:30.478 -07 000000512 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:30.478 -07 000000608 INF: FPGA: Recv YRDPOS 131189 17-03-18 00:08:30.488 -07 000000448 INF: FPGA: Xmit ZDACW 38438\n [Z Motor] 17-03-18 00:08:30.488 -07 000000512 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 17 ms overall 17-03-18 00:08:30.488 -07 000000512 INF: Y Motor: Encoder = 131189 = motor step 102491.4 = 64.0571 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:30.488 -07 000000512 INF: Y Motor: Last commanded position = 64.0626 mm (102500.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:30.488 -07 000000512 INF: Y Motor: Moving 1337 steps-11 17-03-18 00:08:30.488 -07 000000486 INF: FPGA: Recv ZDACW 17-03-18 00:08:30.498 -07 000000448 INF: FPGA: 'ZDACW 38438' command took 0 ms to send, 0 ms to get response, and 26 ms overall 17-03-18 00:08:30.498 -07 000000448 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:30.498 -07 000000608 INF: FPGA: Recv ZDACR 38438 17-03-18 00:08:30.508 -07 000000448 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:30.508 -07 000000448 INF: Z Motor: MoveAbsStepped took 37 ms (did NOT wait for settling yet)) 17-03-18 00:08:30.508 -07 000000512 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:30.668 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 398 ms. 17-03-18 00:08:30.668 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:30.668 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:30.668 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) end. Duration was 397 ms. 17-03-18 00:08:30.668 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 397 ms. 17-03-18 00:08:30.668 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:30.668 -07 000000627 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:08:30.668 -07 000000476 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 194 ms to get response, and 203 ms overall 17-03-18 00:08:30.668 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:08:30.668 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 407 ms. 17-03-18 00:08:30.678 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 6 ms. 17-03-18 00:08:30.678 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 415 ms. 17-03-18 00:08:30.888 -07 000000627 INF: FPGA: Recv YMVTILE 17-03-18 00:08:30.888 -07 000000512 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 370 ms to get response, and 399 ms overall 17-03-18 00:08:30.928 -07 000000512 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:30.928 -07 000000486 INF: FPGA: Recv YRDPOS 132871 17-03-18 00:08:30.938 -07 000000512 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:30.938 -07 000000512 INF: Y Motor: Encoder = 132871 = motor step 103805.5 = 64.8784 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:30.938 -07 000000512 INF: Y Motor: Position error before settling (table move) = -29 encoder counts (-0.014 mm) 17-03-18 00:08:30.938 -07 000000512 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 399 ms, distance = 0.835 mm, average velocity = 2.094 mm/sec 17-03-18 00:08:31.888 -07 000000512 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:31.888 -07 000000476 INF: FPGA: Recv YRDPOS 132872 17-03-18 00:08:31.898 -07 000000512 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:31.898 -07 000000512 INF: Y Motor: Encoder = 132872 = motor step 103806.3 = 64.8789 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:31.918 -07 000000512 INF: Y Motor: After move to 64.892609, error = -0.013702, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:31.918 -07 000000512 INF: Y Motor: Position error after settling (table move) = -28 encoder counts (-0.014 mm), Delta during settling = 1 counts (0.000 mm) 17-03-18 00:08:31.918 -07 000000512 INF: Y Motor: Moved to 64.8789 after 1454 ms (table move) 17-03-18 00:08:31.928 -07 000000586 INF: Z Motor: EndOfMoveAbsStepped: target = -0.175958 mm (DAC = 38438) 17-03-18 00:08:31.928 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.928 -07 000000627 INF: FPGA: Recv ZSGSRD 36687 17-03-18 00:08:31.938 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:31.938 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.938 -07 000000476 INF: FPGA: Recv ZSGSRD 36687 17-03-18 00:08:31.948 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:31.948 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.948 -07 000000478 INF: FPGA: Recv ZSGSRD 36683 17-03-18 00:08:31.958 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:31.958 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.958 -07 000000482 INF: FPGA: Recv ZSGSRD 36676 17-03-18 00:08:31.968 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:31.968 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.968 -07 000000382 INF: FPGA: Recv ZSGSRD 36675 17-03-18 00:08:31.978 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:31.978 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.978 -07 000000482 INF: FPGA: Recv ZSGSRD 36678 17-03-18 00:08:31.988 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:31.988 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.988 -07 000000476 INF: FPGA: Recv ZSGSRD 36686 17-03-18 00:08:31.998 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:31.998 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:31.998 -07 000000627 INF: FPGA: Recv ZSGSRD 36679 17-03-18 00:08:32.008 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:32.008 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:32.008 -07 000000512 INF: FPGA: Recv ZSGSRD 36673 17-03-18 00:08:32.018 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:32.018 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:32.018 -07 000000448 INF: FPGA: Recv ZSGSRD 36684 17-03-18 00:08:32.028 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:32.028 -07 000000586 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:32.028 -07 000000478 INF: FPGA: Recv ZPIEZORD 32756 17-03-18 00:08:32.038 -07 000000586 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:32.038 -07 000000586 INF: Z Motor: Piezo is near 50.0 V (nominally 0 to 100 V), expect near 58.7 V, since DAC is at 5.865 V. Piezo Error = -8.7 V. 17-03-18 00:08:32.038 -07 000000586 INF: Z Motor: ReadAdcAverage: Commanded Z = -176.0 um, Read 10 ADC samples: Avg = 36681 (-175.1 uncal um), Error = 0.9 uncal um, Median = 36681 (-175.1 uncal um), StdDev = 5 (0.0 uncal um), Range = 14 (0.1 uncal um), Min = 36673 (-175.0 uncal um), Max = 36687 (-175.1 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 8 (0.1 uncal um) 17-03-18 00:08:32.038 -07 000000586 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 36681, posNoise = 6, negNoise = 8 17-03-18 00:08:32.038 -07 000000586 INF: Z Motor: After move to -0.175958, error = -0.000012, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:32.038 -07 000000586 INF: Z Motor: Settle wait time = 1456 ms, measure time = 111 ms (allowed 150 ms), total time = 1567 ms 17-03-18 00:08:32.038 -07 000000586 INF: Z Motor: Waiting extra 39 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:32.078 -07 000000586 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1611 ms 17-03-18 00:08:32.078 -07 000000586 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:32.078 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:32.078 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:32.078 -07 000000478 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:32.078 -07 000000608 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:08:32.078 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:32.078 -07 000000586 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:32.078 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:32.078 -07 000000608 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:08:32.088 -07 000000608 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:08:32.088 -07 000000608 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:08:32.098 -07 000000608 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 11 ms. 17-03-18 00:08:32.098 -07 000000608 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 15 ms. 17-03-18 00:08:32.098 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:08:32.138 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:32.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:32.148 -07 000000486 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:08:32.448 -07 000000486 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:08:32.448 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:08:32.448 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:08:32.448 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:08:32.478 -07 000000476 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:08:32.488 -07 000000608 INF: FPGA: Recv CAMSNAP 17-03-18 00:08:32.488 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 380 ms to get response, and 384 ms overall 17-03-18 00:08:32.558 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 114 ms. 17-03-18 00:08:32.558 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:32.558 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:08:32.558 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 117 ms. 17-03-18 00:08:32.588 -07 000000478 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:08:32.588 -07 000000486 INF: Autofocus start 17-03-18 00:08:32.588 -07 000000486 INF: MeasureProfile start 17-03-18 00:08:32.588 -07 000000448 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:08:32.598 -07 000000478 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:08:32.598 -07 000000478 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:08:32.598 -07 000000486 INF: MeasureProfile done 17-03-18 00:08:32.598 -07 000000486 INF: X=-64 Z correction [um]=-0.4038377 17-03-18 00:08:32.598 -07 000000486 INF: Autofocus finished 17-03-18 00:08:32.798 -07 000000586 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:08:32.798 -07 000000478 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:08:32.798 -07 000000610 INF: ------------ Scan Snapping tile 7 at Y-position 64.87890625, Z-position -0.175969877583069. ---------- 17-03-18 00:08:32.798 -07 000000610 INF: Cycle 82 Tile 7 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:32.808 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36862, sync = True, waitForSettling = True) 17-03-18 00:08:32.808 -07 000000610 INF: Z Motor: Moving to -0.168743 mm, started at -0.175970 mm, distance = 7.23 um 17-03-18 00:08:32.808 -07 000000610 INF: FPGA: Xmit ZDACW 36862\n [Z Motor] 17-03-18 00:08:32.808 -07 000000448 INF: FPGA: Recv ZDACW 17-03-18 00:08:32.818 -07 000000610 INF: FPGA: 'ZDACW 36862' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:32.818 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:32.818 -07 000000476 INF: FPGA: Recv ZDACR 36862 17-03-18 00:08:32.828 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:32.828 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:32.978 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:32.978 -07 000000382 INF: FPGA: Recv ZSGSRD 35557 17-03-18 00:08:32.988 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:32.988 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:32.988 -07 000000627 INF: FPGA: Recv ZSGSRD 35558 17-03-18 00:08:32.998 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:32.998 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:32.998 -07 000000382 INF: FPGA: Recv ZSGSRD 35558 17-03-18 00:08:33.008 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.008 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:33.008 -07 000000476 INF: FPGA: Recv ZSGSRD 35557 17-03-18 00:08:33.018 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.018 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:33.018 -07 000000382 INF: FPGA: Recv ZSGSRD 35560 17-03-18 00:08:33.028 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.028 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:33.028 -07 000000382 INF: FPGA: Recv ZSGSRD 35565 17-03-18 00:08:33.038 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.038 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:33.038 -07 000000476 INF: FPGA: Recv ZSGSRD 35554 17-03-18 00:08:33.048 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:33.048 -07 000000586 INF: FPGA: Recv ZSGSRD 35558 17-03-18 00:08:33.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:33.058 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:33.058 -07 000000482 INF: FPGA: Recv ZSGSRD 35562 17-03-18 00:08:33.068 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.068 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:33.068 -07 000000586 INF: FPGA: Recv ZSGSRD 35551 17-03-18 00:08:33.078 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.078 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:33.078 -07 000000586 INF: FPGA: Recv ZPIEZORD 31290 17-03-18 00:08:33.088 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:33.088 -07 000000610 INF: Z Motor: Piezo is near 46.8 V (nominally 0 to 100 V), expect near 56.2 V, since DAC is at 5.625 V. Piezo Error = -9.4 V. 17-03-18 00:08:33.088 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -168.7 um, Read 10 ADC samples: Avg = 35558 (-167.9 uncal um), Error = 0.9 uncal um, Median = 35558 (-167.9 uncal um), StdDev = 4 (0.0 uncal um), Range = 14 (0.1 uncal um), Min = 35551 (-167.8 uncal um), Max = 35565 (-167.9 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:08:33.088 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35558, posNoise = 7, negNoise = 7 17-03-18 00:08:33.088 -07 000000610 INF: Z Motor: After move to -0.168743, error = -0.000012, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:33.088 -07 000000610 INF: Z Motor: MoveAbsStepped took 281 ms) 17-03-18 00:08:33.088 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:33.088 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:33.088 -07 000000486 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:33.088 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:33.088 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:33.088 -07 000000382 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:33.088 -07 000000608 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:33.088 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:33.088 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:08:33.088 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:33.088 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:08:33.088 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:33.088 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:08:33.088 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:33.088 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:08:33.088 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:33.098 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 8 ms. 17-03-18 00:08:33.098 -07 000000486 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 13 ms. 17-03-18 00:08:33.098 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 9 ms. 17-03-18 00:08:33.098 -07 000000608 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 14 ms. 17-03-18 00:08:33.108 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 37039 150\n [Camera] 17-03-18 00:08:33.308 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:34.348 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:34.348 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:34.348 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:34.348 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:34.348 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:08:34.348 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:34.358 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:34.358 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:34.358 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:34.358 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:08:34.538 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 37039 1422 17-03-18 00:08:34.688 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:34.748 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:08:34.748 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:34.748 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:34.748 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:34.748 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:08:34.748 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 397 ms. 17-03-18 00:08:34.748 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 397 ms. 17-03-18 00:08:34.748 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:34.758 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:34.758 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 407 ms. 17-03-18 00:08:35.188 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:35.188 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:35.188 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:35.308 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:35.308 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:35.308 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:35.308 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:35.308 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) start. 17-03-18 00:08:35.308 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:35.308 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:08:35.328 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:08:35.378 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:35.378 -07 000000448 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:35.378 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 37039 150' command took 2 ms to send, 2265 ms to get response, and 2272 ms overall 17-03-18 00:08:35.378 -07 000000610 INF: Skipping AutoFocus in Tile 8: adopting Best-Z from the cache: -0.169344 17-03-18 00:08:35.378 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 65.7226 mm, moving Z to -0.16934 mm 17-03-18 00:08:35.378 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:35.388 -07 000000486 INF: Y Motor: Entering MoveAbsStepped (target = 105156 steps, sync = True, waitForSettling = True) 17-03-18 00:08:35.388 -07 000000486 INF: Y Motor: Moving to 65.7226 mm, started at 64.8789 mm, delta = 0.8437 mm 17-03-18 00:08:35.388 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:35.388 -07 000000448 INF: Z Motor: Entering MoveAbsStepped (DAC = 36993, sync = True, waitForSettling = False) 17-03-18 00:08:35.388 -07 000000448 INF: Z Motor: Moving to -0.169343 mm, started at -0.168755 mm, distance = -0.59 um 17-03-18 00:08:35.388 -07 000000627 INF: FPGA: Recv YRDPOS 132872 17-03-18 00:08:35.398 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:35.398 -07 000000486 INF: Y Motor: Encoder = 132872 = motor step 103806.3 = 64.8789 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:35.398 -07 000000486 INF: Y Motor: Last commanded position = 64.8926 mm (103828.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:35.398 -07 000000486 INF: Y Motor: Moving 1350 steps-29 17-03-18 00:08:35.398 -07 000000448 INF: FPGA: Xmit ZDACW 36993\n [Z Motor] 17-03-18 00:08:35.398 -07 000000547 INF: FPGA: Recv ZDACW 17-03-18 00:08:35.408 -07 000000448 INF: FPGA: 'ZDACW 36993' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:35.408 -07 000000486 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:35.418 -07 000000448 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:35.418 -07 000000012 INF: FPGA: Recv ZDACR 36993 17-03-18 00:08:35.428 -07 000000448 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:35.428 -07 000000448 INF: Z Motor: MoveAbsStepped took 39 ms (did NOT wait for settling yet)) 17-03-18 00:08:35.698 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 388 ms. 17-03-18 00:08:35.698 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 388 ms. 17-03-18 00:08:35.698 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:35.708 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:08:35.708 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:35.708 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:35.708 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:35.708 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:08:35.708 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 521 ms. 17-03-18 00:08:35.718 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 409 ms. 17-03-18 00:08:35.778 -07 000000512 INF: FPGA: Recv YMVTILE 17-03-18 00:08:35.778 -07 000000486 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 387 ms overall 17-03-18 00:08:35.828 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:35.828 -07 000000382 INF: FPGA: Recv YRDPOS 134582 17-03-18 00:08:35.838 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:35.838 -07 000000486 INF: Y Motor: Encoder = 134582 = motor step 105142.2 = 65.7139 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:35.838 -07 000000486 INF: Y Motor: Position error before settling (table move) = -18 encoder counts (-0.009 mm) 17-03-18 00:08:35.838 -07 000000486 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 387 ms, distance = 0.844 mm, average velocity = 2.180 mm/sec 17-03-18 00:08:36.788 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:36.788 -07 000000627 INF: FPGA: Recv YRDPOS 134582 17-03-18 00:08:36.798 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:36.798 -07 000000486 INF: Y Motor: Encoder = 134582 = motor step 105142.2 = 65.7139 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:36.828 -07 000000486 INF: Y Motor: After move to 65.722610, error = -0.008743, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:36.828 -07 000000486 INF: Y Motor: Position error after settling (table move) = -18 encoder counts (-0.009 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:36.828 -07 000000486 INF: Y Motor: Moved to 65.7139 after 1440 ms (table move) 17-03-18 00:08:36.828 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.169343 mm (DAC = 36993) 17-03-18 00:08:36.828 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.828 -07 000000627 INF: FPGA: Recv ZSGSRD 35657 17-03-18 00:08:36.838 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:08:36.838 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.838 -07 000000478 INF: FPGA: Recv ZSGSRD 35653 17-03-18 00:08:36.848 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.848 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.848 -07 000000478 INF: FPGA: Recv ZSGSRD 35658 17-03-18 00:08:36.858 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.858 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.858 -07 000000382 INF: FPGA: Recv ZSGSRD 35649 17-03-18 00:08:36.868 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.868 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.868 -07 000000382 INF: FPGA: Recv ZSGSRD 35654 17-03-18 00:08:36.878 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.878 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.878 -07 000000608 INF: FPGA: Recv ZSGSRD 35654 17-03-18 00:08:36.888 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.888 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.888 -07 000000382 INF: FPGA: Recv ZSGSRD 35646 17-03-18 00:08:36.898 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.898 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.898 -07 000000608 INF: FPGA: Recv ZSGSRD 35646 17-03-18 00:08:36.908 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.908 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.908 -07 000000486 INF: FPGA: Recv ZSGSRD 35648 17-03-18 00:08:36.918 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:36.918 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:36.918 -07 000000547 INF: FPGA: Recv ZSGSRD 35655 17-03-18 00:08:36.928 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.928 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:36.928 -07 000000448 INF: FPGA: Recv ZPIEZORD 31390 17-03-18 00:08:36.938 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:36.938 -07 000000610 INF: Z Motor: Piezo is near 47.1 V (nominally 0 to 100 V), expect near 56.4 V, since DAC is at 5.645 V. Piezo Error = -9.4 V. 17-03-18 00:08:36.938 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -169.3 um, Read 10 ADC samples: Avg = 35652 (-168.5 uncal um), Error = 0.9 uncal um, Median = 35654 (-168.5 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 35646 (-168.4 uncal um), Max = 35658 (-168.5 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:08:36.938 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35652, posNoise = 6, negNoise = 6 17-03-18 00:08:36.938 -07 000000610 INF: Z Motor: After move to -0.169343, error = -0.000016, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:36.938 -07 000000610 INF: Z Motor: Settle wait time = 1442 ms, measure time = 108 ms (allowed 150 ms), total time = 1550 ms 17-03-18 00:08:36.938 -07 000000610 INF: Z Motor: Waiting extra 42 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:36.988 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1602 ms 17-03-18 00:08:36.988 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:36.988 -07 000000610 INF: ------------ Scan Snapping tile 8 at Y-position 65.7138671875, Z-position -0.169359363289197. ---------- 17-03-18 00:08:36.988 -07 000000610 INF: Cycle 82 Tile 8 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:36.988 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36816, sync = True, waitForSettling = True) 17-03-18 00:08:36.988 -07 000000610 INF: Z Motor: Moving to -0.168533 mm, started at -0.169359 mm, distance = 0.83 um 17-03-18 00:08:36.988 -07 000000610 INF: FPGA: Xmit ZDACW 36816\n [Z Motor] 17-03-18 00:08:36.988 -07 000000512 INF: FPGA: Recv ZDACW 17-03-18 00:08:36.998 -07 000000610 INF: FPGA: 'ZDACW 36816' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:08:36.998 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:36.998 -07 000000448 INF: FPGA: Recv ZDACR 36816 17-03-18 00:08:37.008 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.008 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:37.138 -07 000000608 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:37.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:37.158 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.158 -07 000000512 INF: FPGA: Recv ZSGSRD 35528 17-03-18 00:08:37.168 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:37.168 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.168 -07 000000608 INF: FPGA: Recv ZSGSRD 35530 17-03-18 00:08:37.178 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.178 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.178 -07 000000547 INF: FPGA: Recv ZSGSRD 35528 17-03-18 00:08:37.188 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.188 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.188 -07 000000486 INF: FPGA: Recv ZSGSRD 35532 17-03-18 00:08:37.198 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.198 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.198 -07 000000627 INF: FPGA: Recv ZSGSRD 35526 17-03-18 00:08:37.208 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.208 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.208 -07 000000448 INF: FPGA: Recv ZSGSRD 35526 17-03-18 00:08:37.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.218 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.218 -07 000000486 INF: FPGA: Recv ZSGSRD 35532 17-03-18 00:08:37.228 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:08:37.228 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.228 -07 000000608 INF: FPGA: Recv ZSGSRD 35529 17-03-18 00:08:37.238 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:08:37.238 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.238 -07 000000486 INF: FPGA: Recv ZSGSRD 35524 17-03-18 00:08:37.248 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.248 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:37.248 -07 000000547 INF: FPGA: Recv ZSGSRD 35526 17-03-18 00:08:37.258 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.258 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:37.258 -07 000000627 INF: FPGA: Recv ZPIEZORD 31194 17-03-18 00:08:37.268 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:37.268 -07 000000610 INF: Z Motor: Piezo is near 46.6 V (nominally 0 to 100 V), expect near 56.2 V, since DAC is at 5.618 V. Piezo Error = -9.5 V. 17-03-18 00:08:37.268 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -168.5 um, Read 10 ADC samples: Avg = 35528 (-167.7 uncal um), Error = 0.8 uncal um, Median = 35528 (-167.7 uncal um), StdDev = 3 (0.0 uncal um), Range = 8 (0.1 uncal um), Min = 35524 (-167.7 uncal um), Max = 35532 (-167.7 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:08:37.268 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35528, posNoise = 4, negNoise = 4 17-03-18 00:08:37.268 -07 000000610 INF: Z Motor: After move to -0.168533, error = -0.000030, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:37.268 -07 000000610 INF: Z Motor: MoveAbsStepped took 277 ms) 17-03-18 00:08:37.268 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:37.268 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:37.268 -07 000000382 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:37.268 -07 000000486 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:37.268 -07 000000478 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:37.268 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:37.268 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:37.268 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:37.268 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:37.268 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:37.268 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:08:37.268 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:37.278 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:37.278 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:37.278 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:08:37.278 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:37.288 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:08:37.288 -07 000000486 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:08:37.288 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:08:37.288 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:08:37.298 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36993 150\n [Camera] 17-03-18 00:08:37.488 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:38.528 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:38.528 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:38.528 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:38.528 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:38.528 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:08:38.538 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:38.538 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:38.538 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:38.538 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:38.538 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) start. 17-03-18 00:08:38.728 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36993 1422 17-03-18 00:08:38.868 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:38.928 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:08:38.928 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:38.928 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:38.938 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:08:38.938 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:08:38.938 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:38.938 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:38.938 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:08:38.938 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:38.948 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:08:39.378 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:39.378 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:39.378 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:39.378 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:39.378 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) start. 17-03-18 00:08:39.378 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:39.378 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:39.378 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:39.378 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:39.378 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) start. 17-03-18 00:08:39.568 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:39.568 -07 000000478 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:39.568 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36993 150' command took 2 ms to send, 2262 ms to get response, and 2272 ms overall 17-03-18 00:08:39.568 -07 000000610 INF: Skipping AutoFocus in Tile 9: adopting Best-Z from the cache: -0.168886 17-03-18 00:08:39.568 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 66.5526 mm, moving Z to -0.16889 mm 17-03-18 00:08:39.568 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:39.568 -07 000000608 INF: Y Motor: Entering MoveAbsStepped (target = 106484 steps, sync = True, waitForSettling = True) 17-03-18 00:08:39.688 -07 000000608 INF: Y Motor: Moving to 66.5526 mm, started at 65.7139 mm, delta = 0.8387 mm 17-03-18 00:08:39.688 -07 000000608 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:39.688 -07 000000448 INF: Z Motor: Entering MoveAbsStepped (DAC = 36893, sync = True, waitForSettling = False) 17-03-18 00:08:39.688 -07 000000448 INF: Z Motor: Moving to -0.168885 mm, started at -0.168563 mm, distance = -0.32 um 17-03-18 00:08:39.688 -07 000000478 INF: FPGA: Recv YRDPOS 134582 17-03-18 00:08:39.698 -07 000000608 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:08:39.698 -07 000000608 INF: Y Motor: Encoder = 134582 = motor step 105142.2 = 65.7139 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:39.698 -07 000000608 INF: Y Motor: Last commanded position = 65.7226 mm (105156.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:39.698 -07 000000608 INF: Y Motor: Moving 1342 steps-18 17-03-18 00:08:39.698 -07 000000448 INF: FPGA: Xmit ZDACW 36893\n [Z Motor] 17-03-18 00:08:39.698 -07 000000512 INF: FPGA: Recv ZDACW 17-03-18 00:08:39.708 -07 000000448 INF: FPGA: 'ZDACW 36893' command took 1 ms to send, 0 ms to get response, and 15 ms overall 17-03-18 00:08:39.708 -07 000000448 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:39.708 -07 000000547 INF: FPGA: Recv ZDACR 36893 17-03-18 00:08:39.718 -07 000000608 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:39.718 -07 000000448 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:39.718 -07 000000448 INF: Z Motor: MoveAbsStepped took 26 ms (did NOT wait for settling yet)) 17-03-18 00:08:39.778 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) end. Duration was 401 ms. 17-03-18 00:08:39.778 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 401 ms. 17-03-18 00:08:39.778 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:39.778 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:08:39.778 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 401 ms. 17-03-18 00:08:39.778 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:39.778 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:39.778 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 406 ms. 17-03-18 00:08:39.778 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:39.788 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 412 ms. 17-03-18 00:08:40.088 -07 000000512 INF: FPGA: Recv YMVTILE 17-03-18 00:08:40.088 -07 000000608 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 397 ms overall 17-03-18 00:08:40.138 -07 000000608 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:40.138 -07 000000382 INF: FPGA: Recv YRDPOS 136272 17-03-18 00:08:40.148 -07 000000608 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:40.148 -07 000000608 INF: Y Motor: Encoder = 136272 = motor step 106462.5 = 66.5391 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:40.148 -07 000000608 INF: Y Motor: Position error before settling (table move) = -28 encoder counts (-0.014 mm) 17-03-18 00:08:40.148 -07 000000608 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 397 ms, distance = 0.839 mm, average velocity = 2.113 mm/sec 17-03-18 00:08:40.688 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:08:41.098 -07 000000608 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:41.098 -07 000000627 INF: FPGA: Recv YRDPOS 136272 17-03-18 00:08:41.108 -07 000000608 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:41.108 -07 000000608 INF: Y Motor: Encoder = 136272 = motor step 106462.5 = 66.5391 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:41.128 -07 000000608 INF: Y Motor: After move to 66.552605, error = -0.013542, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:41.128 -07 000000608 INF: Y Motor: Position error after settling (table move) = -28 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:41.128 -07 000000608 INF: Y Motor: Moved to 66.5391 after 1445 ms (table move) 17-03-18 00:08:41.138 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.168885 mm (DAC = 36893) 17-03-18 00:08:41.138 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.138 -07 000000547 INF: FPGA: Recv ZSGSRD 35575 17-03-18 00:08:41.148 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:41.148 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.148 -07 000000482 INF: FPGA: Recv ZSGSRD 35581 17-03-18 00:08:41.158 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.158 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.158 -07 000000547 INF: FPGA: Recv ZSGSRD 35578 17-03-18 00:08:41.168 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.168 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.168 -07 000000486 INF: FPGA: Recv ZSGSRD 35584 17-03-18 00:08:41.178 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.178 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.178 -07 000000547 INF: FPGA: Recv ZSGSRD 35580 17-03-18 00:08:41.188 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.188 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.188 -07 000000486 INF: FPGA: Recv ZSGSRD 35580 17-03-18 00:08:41.198 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.198 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.198 -07 000000012 INF: FPGA: Recv ZSGSRD 35580 17-03-18 00:08:41.208 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.208 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.208 -07 000000627 INF: FPGA: Recv ZSGSRD 35569 17-03-18 00:08:41.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.218 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.218 -07 000000608 INF: FPGA: Recv ZSGSRD 35578 17-03-18 00:08:41.228 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.228 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.228 -07 000000627 INF: FPGA: Recv ZSGSRD 35585 17-03-18 00:08:41.238 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.238 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:41.238 -07 000000012 INF: FPGA: Recv ZPIEZORD 31320 17-03-18 00:08:41.248 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.248 -07 000000610 INF: Z Motor: Piezo is near 46.9 V (nominally 0 to 100 V), expect near 56.3 V, since DAC is at 5.630 V. Piezo Error = -9.4 V. 17-03-18 00:08:41.248 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -168.9 um, Read 10 ADC samples: Avg = 35579 (-168.0 uncal um), Error = 0.9 uncal um, Median = 35580 (-168.0 uncal um), StdDev = 5 (0.0 uncal um), Range = 16 (0.1 uncal um), Min = 35569 (-168.0 uncal um), Max = 35585 (-168.1 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 10 (0.1 uncal um) 17-03-18 00:08:41.248 -07 000000610 INF: Z Motor: ADC noise = 10, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35579, posNoise = 6, negNoise = 10 17-03-18 00:08:41.248 -07 000000610 INF: Z Motor: After move to -0.168885, error = -0.000005, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:41.248 -07 000000610 INF: Z Motor: Settle wait time = 1447 ms, measure time = 109 ms (allowed 150 ms), total time = 1556 ms 17-03-18 00:08:41.248 -07 000000610 INF: Z Motor: Waiting extra 41 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:41.298 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1725 ms 17-03-18 00:08:41.298 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:41.298 -07 000000610 INF: ------------ Scan Snapping tile 9 at Y-position 66.5390625, Z-position -0.168890395802848. ---------- 17-03-18 00:08:41.298 -07 000000610 INF: Cycle 82 Tile 9 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:41.298 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36716, sync = True, waitForSettling = True) 17-03-18 00:08:41.298 -07 000000610 INF: Z Motor: Moving to -0.168075 mm, started at -0.168890 mm, distance = 0.82 um 17-03-18 00:08:41.298 -07 000000610 INF: FPGA: Xmit ZDACW 36716\n [Z Motor] 17-03-18 00:08:41.298 -07 000000608 INF: FPGA: Recv ZDACW 17-03-18 00:08:41.308 -07 000000610 INF: FPGA: 'ZDACW 36716' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:08:41.308 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:41.308 -07 000000486 INF: FPGA: Recv ZDACR 36716 17-03-18 00:08:41.318 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.318 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:41.468 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.468 -07 000000608 INF: FPGA: Recv ZSGSRD 35456 17-03-18 00:08:41.478 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.478 -07 000000012 INF: FPGA: Recv ZSGSRD 35455 17-03-18 00:08:41.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.488 -07 000000627 INF: FPGA: Recv ZSGSRD 35454 17-03-18 00:08:41.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.498 -07 000000486 INF: FPGA: Recv ZSGSRD 35452 17-03-18 00:08:41.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.508 -07 000000482 INF: FPGA: Recv ZSGSRD 35449 17-03-18 00:08:41.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.518 -07 000000608 INF: FPGA: Recv ZSGSRD 35456 17-03-18 00:08:41.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.528 -07 000000482 INF: FPGA: Recv ZSGSRD 35456 17-03-18 00:08:41.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.538 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.538 -07 000000627 INF: FPGA: Recv ZSGSRD 35450 17-03-18 00:08:41.548 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.548 -07 000000482 INF: FPGA: Recv ZSGSRD 35455 17-03-18 00:08:41.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:41.558 -07 000000012 INF: FPGA: Recv ZSGSRD 35455 17-03-18 00:08:41.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.568 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:41.568 -07 000000486 INF: FPGA: Recv ZPIEZORD 31166 17-03-18 00:08:41.578 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:41.578 -07 000000610 INF: Z Motor: Piezo is near 46.6 V (nominally 0 to 100 V), expect near 56.0 V, since DAC is at 5.603 V. Piezo Error = -9.4 V. 17-03-18 00:08:41.578 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -168.1 um, Read 10 ADC samples: Avg = 35454 (-167.2 uncal um), Error = 0.9 uncal um, Median = 35455 (-167.2 uncal um), StdDev = 3 (0.0 uncal um), Range = 7 (0.0 uncal um), Min = 35449 (-167.2 uncal um), Max = 35456 (-167.2 uncal um), Pos Noise = 2 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:08:41.578 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35454, posNoise = 2, negNoise = 5 17-03-18 00:08:41.578 -07 000000610 INF: Z Motor: After move to -0.168075, error = -0.000012, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:41.578 -07 000000610 INF: Z Motor: MoveAbsStepped took 274 ms) 17-03-18 00:08:41.578 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:41.578 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:41.578 -07 000000547 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:41.578 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:41.578 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:41.578 -07 000000486 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:41.578 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:41.578 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:08:41.578 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:41.578 -07 000000608 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:41.578 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 2 ms. 17-03-18 00:08:41.578 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:41.578 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:08:41.588 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:41.588 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:08:41.588 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:41.588 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 8 ms. 17-03-18 00:08:41.588 -07 000000486 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 14 ms. 17-03-18 00:08:41.588 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 9 ms. 17-03-18 00:08:41.588 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 15 ms. 17-03-18 00:08:41.598 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36893 150\n [Camera] 17-03-18 00:08:41.798 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:42.138 -07 000000482 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:42.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:42.828 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:42.828 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:42.838 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:42.838 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:42.838 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:42.838 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:42.838 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:42.838 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:08:42.838 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:42.838 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) start. 17-03-18 00:08:43.028 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36893 1422 17-03-18 00:08:43.178 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:43.238 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 398 ms. 17-03-18 00:08:43.238 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 398 ms. 17-03-18 00:08:43.238 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:43.238 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) end. Duration was 396 ms. 17-03-18 00:08:43.238 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 396 ms. 17-03-18 00:08:43.238 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:43.238 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:43.238 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:43.238 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 408 ms. 17-03-18 00:08:43.248 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 409 ms. 17-03-18 00:08:43.678 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:43.678 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:43.678 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:43.678 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:43.678 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) start. 17-03-18 00:08:43.678 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:43.678 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:43.678 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:43.678 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:43.678 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) start. 17-03-18 00:08:43.868 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:43.868 -07 000000586 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:43.868 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36893 150' command took 2 ms to send, 2263 ms to get response, and 2272 ms overall 17-03-18 00:08:43.868 -07 000000610 INF: Skipping AutoFocus in Tile 10: adopting Best-Z from the cache: -0.168835 17-03-18 00:08:43.868 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 67.3826 mm, moving Z to -0.16884 mm 17-03-18 00:08:43.868 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:43.868 -07 000000382 INF: Y Motor: Entering MoveAbsStepped (target = 107812 steps, sync = True, waitForSettling = True) 17-03-18 00:08:43.868 -07 000000382 INF: Y Motor: Moving to 67.3826 mm, started at 66.5391 mm, delta = 0.8435 mm 17-03-18 00:08:43.868 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:43.868 -07 000000012 INF: Z Motor: Entering MoveAbsStepped (DAC = 36882, sync = True, waitForSettling = False) 17-03-18 00:08:43.868 -07 000000012 INF: Z Motor: Moving to -0.168835 mm, started at -0.168087 mm, distance = -0.75 um 17-03-18 00:08:43.878 -07 000000012 INF: FPGA: Xmit ZDACW 36882\n [Z Motor] 17-03-18 00:08:43.878 -07 000000608 INF: FPGA: Recv YRDPOS 136272 17-03-18 00:08:43.878 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 2 ms overall 17-03-18 00:08:43.878 -07 000000382 INF: Y Motor: Encoder = 136272 = motor step 106462.5 = 66.5391 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:43.878 -07 000000382 INF: Y Motor: Last commanded position = 66.5526 mm (106484.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:43.878 -07 000000382 INF: Y Motor: Moving 1350 steps-28 17-03-18 00:08:43.878 -07 000000608 INF: FPGA: Recv ZDACW 17-03-18 00:08:43.888 -07 000000012 INF: FPGA: 'ZDACW 36882' command took 0 ms to send, 0 ms to get response, and 11 ms overall 17-03-18 00:08:43.888 -07 000000382 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:43.898 -07 000000012 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:43.898 -07 000000476 INF: FPGA: Recv ZDACR 36882 17-03-18 00:08:43.908 -07 000000012 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:43.908 -07 000000012 INF: Z Motor: MoveAbsStepped took 31 ms (did NOT wait for settling yet)) 17-03-18 00:08:44.058 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) end. Duration was 379 ms. 17-03-18 00:08:44.058 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 379 ms. 17-03-18 00:08:44.058 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:44.058 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:44.068 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 389 ms. 17-03-18 00:08:44.068 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) end. Duration was 394 ms. 17-03-18 00:08:44.068 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:08:44.068 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:44.078 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:44.078 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 398 ms. 17-03-18 00:08:44.258 -07 000000482 INF: FPGA: Recv YMVTILE 17-03-18 00:08:44.258 -07 000000382 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 386 ms overall 17-03-18 00:08:44.298 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:44.308 -07 000000012 INF: FPGA: Recv YRDPOS 137977 17-03-18 00:08:44.308 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 2 ms to get response, and 3 ms overall 17-03-18 00:08:44.308 -07 000000382 INF: Y Motor: Encoder = 137977 = motor step 107794.5 = 67.3716 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:44.308 -07 000000382 INF: Y Motor: Position error before settling (table move) = -23 encoder counts (-0.011 mm) 17-03-18 00:08:44.308 -07 000000382 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 387 ms, distance = 0.844 mm, average velocity = 2.180 mm/sec 17-03-18 00:08:45.258 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:45.258 -07 000000486 INF: FPGA: Recv YRDPOS 137977 17-03-18 00:08:45.268 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:45.268 -07 000000382 INF: Y Motor: Encoder = 137977 = motor step 107794.5 = 67.3716 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:45.298 -07 000000382 INF: Y Motor: After move to 67.382607, error = -0.011024, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:45.298 -07 000000382 INF: Y Motor: Position error after settling (table move) = -23 encoder counts (-0.011 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:45.298 -07 000000382 INF: Y Motor: Moved to 67.3716 after 1426 ms (table move) 17-03-18 00:08:45.298 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.168835 mm (DAC = 36882) 17-03-18 00:08:45.298 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.308 -07 000000382 INF: FPGA: Recv ZSGSRD 35583 17-03-18 00:08:45.308 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 1 ms to get response, and 2 ms overall 17-03-18 00:08:45.308 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.308 -07 000000382 INF: FPGA: Recv ZSGSRD 35570 17-03-18 00:08:45.318 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:45.318 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.318 -07 000000586 INF: FPGA: Recv ZSGSRD 35572 17-03-18 00:08:45.328 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.328 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.328 -07 000000608 INF: FPGA: Recv ZSGSRD 35570 17-03-18 00:08:45.338 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.338 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.338 -07 000000608 INF: FPGA: Recv ZSGSRD 35569 17-03-18 00:08:45.348 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.348 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.348 -07 000000608 INF: FPGA: Recv ZSGSRD 35575 17-03-18 00:08:45.358 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.358 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.358 -07 000000586 INF: FPGA: Recv ZSGSRD 35573 17-03-18 00:08:45.368 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.368 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.368 -07 000000608 INF: FPGA: Recv ZSGSRD 35565 17-03-18 00:08:45.378 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.378 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.378 -07 000000586 INF: FPGA: Recv ZSGSRD 35570 17-03-18 00:08:45.388 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.388 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.388 -07 000000476 INF: FPGA: Recv ZSGSRD 35573 17-03-18 00:08:45.398 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.398 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:45.398 -07 000000012 INF: FPGA: Recv ZPIEZORD 31332 17-03-18 00:08:45.408 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.408 -07 000000610 INF: Z Motor: Piezo is near 46.9 V (nominally 0 to 100 V), expect near 56.3 V, since DAC is at 5.628 V. Piezo Error = -9.3 V. 17-03-18 00:08:45.408 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -168.8 um, Read 10 ADC samples: Avg = 35572 (-168.0 uncal um), Error = 0.9 uncal um, Median = 35571 (-168.0 uncal um), StdDev = 5 (0.0 uncal um), Range = 18 (0.1 uncal um), Min = 35565 (-167.9 uncal um), Max = 35583 (-168.0 uncal um), Pos Noise = 11 (0.1 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:08:45.408 -07 000000610 INF: Z Motor: ADC noise = 11, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35572, posNoise = 11, negNoise = 7 17-03-18 00:08:45.408 -07 000000610 INF: Z Motor: After move to -0.168835, error = -0.000010, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:45.408 -07 000000610 INF: Z Motor: Settle wait time = 1429 ms, measure time = 103 ms (allowed 150 ms), total time = 1532 ms 17-03-18 00:08:45.408 -07 000000610 INF: Z Motor: Waiting extra 47 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:45.458 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1584 ms 17-03-18 00:08:45.458 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:45.458 -07 000000610 INF: ------------ Scan Snapping tile 10 at Y-position 67.37158203125, Z-position -0.168845426317856. ---------- 17-03-18 00:08:45.458 -07 000000610 INF: Cycle 82 Tile 10 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:45.458 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36705, sync = True, waitForSettling = True) 17-03-18 00:08:45.458 -07 000000610 INF: Z Motor: Moving to -0.168025 mm, started at -0.168845 mm, distance = 0.82 um 17-03-18 00:08:45.458 -07 000000610 INF: FPGA: Xmit ZDACW 36705\n [Z Motor] 17-03-18 00:08:45.458 -07 000000482 INF: FPGA: Recv ZDACW 17-03-18 00:08:45.468 -07 000000610 INF: FPGA: 'ZDACW 36705' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:08:45.468 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:45.468 -07 000000486 INF: FPGA: Recv ZDACR 36705 17-03-18 00:08:45.478 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.478 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:45.628 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.628 -07 000000486 INF: FPGA: Recv ZSGSRD 35440 17-03-18 00:08:45.638 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:45.638 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.638 -07 000000448 INF: FPGA: Recv ZSGSRD 35452 17-03-18 00:08:45.648 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.648 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.648 -07 000000627 INF: FPGA: Recv ZSGSRD 35444 17-03-18 00:08:45.658 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.658 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.658 -07 000000448 INF: FPGA: Recv ZSGSRD 35445 17-03-18 00:08:45.668 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.668 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.668 -07 000000448 INF: FPGA: Recv ZSGSRD 35447 17-03-18 00:08:45.678 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.678 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.678 -07 000000448 INF: FPGA: Recv ZSGSRD 35447 17-03-18 00:08:45.688 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.688 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.688 -07 000000627 INF: FPGA: Recv ZSGSRD 35449 17-03-18 00:08:45.698 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.698 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.698 -07 000000448 INF: FPGA: Recv ZSGSRD 35441 17-03-18 00:08:45.708 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.708 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.708 -07 000000627 INF: FPGA: Recv ZSGSRD 35449 17-03-18 00:08:45.718 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.718 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:45.718 -07 000000627 INF: FPGA: Recv ZSGSRD 35452 17-03-18 00:08:45.728 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:45.738 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:45.738 -07 000000012 INF: FPGA: Recv ZPIEZORD 31167 17-03-18 00:08:45.748 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:45.748 -07 000000610 INF: Z Motor: Piezo is near 46.6 V (nominally 0 to 100 V), expect near 56.0 V, since DAC is at 5.601 V. Piezo Error = -9.4 V. 17-03-18 00:08:45.748 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -168.0 um, Read 10 ADC samples: Avg = 35447 (-167.2 uncal um), Error = 0.9 uncal um, Median = 35447 (-167.2 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 35440 (-167.1 uncal um), Max = 35452 (-167.2 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:08:45.748 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35447, posNoise = 5, negNoise = 7 17-03-18 00:08:45.748 -07 000000610 INF: Z Motor: After move to -0.168025, error = -0.000018, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:45.748 -07 000000610 INF: Z Motor: MoveAbsStepped took 285 ms) 17-03-18 00:08:45.748 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:45.748 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:45.748 -07 000000608 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:45.748 -07 000000586 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:45.748 -07 000000486 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:45.748 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:45.748 -07 000000448 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:45.748 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:45.748 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:08:45.748 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:45.748 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:45.758 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:45.758 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:45.758 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:45.758 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:08:45.758 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:45.768 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 8 ms. 17-03-18 00:08:45.768 -07 000000448 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 13 ms. 17-03-18 00:08:45.768 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 9 ms. 17-03-18 00:08:45.768 -07 000000486 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 15 ms. 17-03-18 00:08:45.788 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36882 150\n [Camera] 17-03-18 00:08:45.978 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:46.068 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:08:47.018 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:47.018 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:47.028 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:47.028 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:47.028 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:47.028 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) start. 17-03-18 00:08:47.028 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:47.028 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:47.028 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:47.028 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2182910056, 0x0, 418435064) start. 17-03-18 00:08:47.138 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:47.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:47.218 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36882 1422 17-03-18 00:08:47.358 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:47.428 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:08:47.428 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:47.428 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:47.428 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2182910056, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:08:47.428 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:08:47.428 -07 000000012 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:47.428 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:08:47.428 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 405 ms. 17-03-18 00:08:47.428 -07 000000012 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:08:47.438 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 413 ms. 17-03-18 00:08:47.868 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:47.868 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:47.868 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:47.868 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:47.868 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 2218332320, 0x0, 391868352) start. 17-03-18 00:08:47.868 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:47.868 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:47.868 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:47.868 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:47.868 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 2281705520, 0x0, 418435064) start. 17-03-18 00:08:48.058 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP 2269 17-03-18 00:08:48.058 -07 000000476 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:48.058 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36882 150' command took 2 ms to send, 2266 ms to get response, and 2290 ms overall 17-03-18 00:08:48.058 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 0 - Best Z for this model is: -0.172880 17-03-18 00:08:48.058 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 11 at Y-position 67.37158203125, Z-Position -0.168042399800136. ------ 17-03-18 00:08:48.068 -07 000000476 INF: Y Motor: MoveYZ optimized starting. Moving Y to 68.2126 mm, moving Z to -0.17439 mm 17-03-18 00:08:48.068 -07 000000476 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:48.068 -07 000000608 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:08:48.068 -07 000000382 INF: Y Motor: Entering MoveAbsStepped (target = 109140 steps, sync = True, waitForSettling = True) 17-03-18 00:08:48.068 -07 000000012 INF: Z Motor: Entering MoveAbsStepped (DAC = 38095, sync = True, waitForSettling = False) 17-03-18 00:08:48.068 -07 000000012 INF: Z Motor: Moving to -0.174388 mm, started at -0.168042 mm, distance = -6.35 um 17-03-18 00:08:48.068 -07 000000382 INF: Y Motor: Moving to 68.2126 mm, started at 67.3716 mm, delta = 0.8410 mm 17-03-18 00:08:48.078 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:48.088 -07 000000012 INF: FPGA: Xmit ZDACW 38095\n [Z Motor] 17-03-18 00:08:48.248 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 2281705520, 0x0, 418435064) end. Duration was 384 ms. 17-03-18 00:08:48.248 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 385 ms. 17-03-18 00:08:48.248 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:48.258 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:48.258 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 2218332320, 0x0, 391868352) end. Duration was 393 ms. 17-03-18 00:08:48.258 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 393 ms. 17-03-18 00:08:48.258 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:48.258 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:48.258 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 397 ms. 17-03-18 00:08:48.258 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 395 ms. 17-03-18 00:08:48.268 -07 000000486 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:08:48.278 -07 000000486 INF: FPGA: Recv YRDPOS 137978 17-03-18 00:08:48.278 -07 000000486 INF: FPGA: Recv ZDACW 17-03-18 00:08:48.278 -07 000000012 INF: FPGA: 'ZDACW 38095' command took 0 ms to send, 180 ms to get response, and 203 ms overall 17-03-18 00:08:48.278 -07 000000608 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 200 ms to get response, and 206 ms overall 17-03-18 00:08:48.278 -07 000000012 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:48.278 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 189 ms to get response, and 203 ms overall 17-03-18 00:08:48.278 -07 000000382 INF: Y Motor: Encoder = 137978 = motor step 107795.3 = 67.3721 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:48.278 -07 000000547 INF: FPGA: Recv ZDACR 38095 17-03-18 00:08:48.278 -07 000000382 INF: Y Motor: Last commanded position = 67.3826 mm (107812.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:48.278 -07 000000382 INF: Y Motor: Moving 1345 steps-23 17-03-18 00:08:48.288 -07 000000012 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:48.288 -07 000000012 INF: Z Motor: MoveAbsStepped took 213 ms (did NOT wait for settling yet)) 17-03-18 00:08:48.288 -07 000000382 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:48.298 -07 000000608 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:08:48.498 -07 000000486 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:08:48.498 -07 000000608 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 192 ms to get response, and 222 ms overall 17-03-18 00:08:48.658 -07 000000547 INF: FPGA: Recv YMVTILE 17-03-18 00:08:48.658 -07 000000382 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 383 ms overall 17-03-18 00:08:48.708 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:48.708 -07 000000486 INF: FPGA: Recv YRDPOS 139684 17-03-18 00:08:48.718 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:48.718 -07 000000382 INF: Y Motor: Encoder = 139684 = motor step 109128.1 = 68.2051 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:48.718 -07 000000382 INF: Y Motor: Position error before settling (table move) = -15 encoder counts (-0.007 mm) 17-03-18 00:08:48.718 -07 000000382 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 383 ms, distance = 0.841 mm, average velocity = 2.196 mm/sec 17-03-18 00:08:49.668 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:49.668 -07 000000012 INF: FPGA: Recv YRDPOS 139684 17-03-18 00:08:49.678 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.678 -07 000000382 INF: Y Motor: Encoder = 139684 = motor step 109128.1 = 68.2051 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:49.698 -07 000000382 INF: Y Motor: After move to 68.212608, error = -0.007530, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:49.698 -07 000000382 INF: Y Motor: Position error after settling (table move) = -15 encoder counts (-0.007 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:49.698 -07 000000382 INF: Y Motor: Moved to 68.2051 after 1629 ms (table move) 17-03-18 00:08:49.698 -07 000000476 INF: Z Motor: EndOfMoveAbsStepped: target = -0.174388 mm (DAC = 38095) 17-03-18 00:08:49.698 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.708 -07 000000012 INF: FPGA: Recv ZSGSRD 36437 17-03-18 00:08:49.708 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 2 ms to get response, and 2 ms overall 17-03-18 00:08:49.708 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.708 -07 000000382 INF: FPGA: Recv ZSGSRD 36433 17-03-18 00:08:49.718 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:08:49.718 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.718 -07 000000512 INF: FPGA: Recv ZSGSRD 36444 17-03-18 00:08:49.728 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.728 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.728 -07 000000382 INF: FPGA: Recv ZSGSRD 36438 17-03-18 00:08:49.738 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.738 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.738 -07 000000512 INF: FPGA: Recv ZSGSRD 36437 17-03-18 00:08:49.748 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.748 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.748 -07 000000608 INF: FPGA: Recv ZSGSRD 36436 17-03-18 00:08:49.758 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.758 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.758 -07 000000012 INF: FPGA: Recv ZSGSRD 36445 17-03-18 00:08:49.768 -07 000000476 INF: FPGA: 'ZSGSRD' command took 1 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:49.768 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.768 -07 000000478 INF: FPGA: Recv ZSGSRD 36440 17-03-18 00:08:49.778 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:49.778 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.778 -07 000000012 INF: FPGA: Recv ZSGSRD 36433 17-03-18 00:08:49.788 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.788 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:49.788 -07 000000547 INF: FPGA: Recv ZSGSRD 36437 17-03-18 00:08:49.798 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.798 -07 000000476 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:49.798 -07 000000478 INF: FPGA: Recv ZPIEZORD 32524 17-03-18 00:08:49.808 -07 000000476 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:49.808 -07 000000476 INF: Z Motor: Piezo is near 49.5 V (nominally 0 to 100 V), expect near 58.1 V, since DAC is at 5.813 V. Piezo Error = -8.6 V. 17-03-18 00:08:49.808 -07 000000476 INF: Z Motor: ReadAdcAverage: Commanded Z = -174.4 um, Read 10 ADC samples: Avg = 36438 (-173.5 uncal um), Error = 0.9 uncal um, Median = 36437 (-173.5 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 36433 (-173.5 uncal um), Max = 36445 (-173.6 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:08:49.808 -07 000000476 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 36438, posNoise = 7, negNoise = 5 17-03-18 00:08:49.808 -07 000000476 INF: Z Motor: After move to -0.174388, error = -0.000021, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:49.808 -07 000000476 INF: Z Motor: Settle wait time = 1632 ms, measure time = 102 ms (allowed 150 ms), total time = 1734 ms 17-03-18 00:08:49.808 -07 000000476 INF: Z Motor: Waiting extra 48 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:49.858 -07 000000476 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1786 ms 17-03-18 00:08:49.858 -07 000000476 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:49.858 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:49.858 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:49.858 -07 000000382 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:49.858 -07 000000608 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:49.858 -07 000000512 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:08:49.858 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:49.858 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:08:49.858 -07 000000512 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:08:49.868 -07 000000512 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:49.868 -07 000000512 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:08:49.868 -07 000000512 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 6 ms. 17-03-18 00:08:49.868 -07 000000512 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 11 ms. 17-03-18 00:08:49.878 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:08:49.918 -07 000000012 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:08:50.218 -07 000000476 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:08:50.218 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:08:50.218 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:08:50.218 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:08:50.258 -07 000000012 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:08:50.258 -07 000000486 INF: FPGA: Recv CAMSNAP 17-03-18 00:08:50.258 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 2 ms to send, 376 ms to get response, and 384 ms overall 17-03-18 00:08:50.338 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 116 ms. 17-03-18 00:08:50.338 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:50.338 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:08:50.338 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 119 ms. 17-03-18 00:08:50.358 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:08:50.358 -07 000000012 INF: Autofocus start 17-03-18 00:08:50.358 -07 000000012 INF: MeasureProfile start 17-03-18 00:08:50.358 -07 000000547 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:08:50.368 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:08:50.368 -07 000000512 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:08:50.368 -07 000000012 INF: MeasureProfile done 17-03-18 00:08:50.368 -07 000000012 INF: X=32 Z correction [um]=0.09220707 17-03-18 00:08:50.368 -07 000000012 INF: Autofocus finished 17-03-18 00:08:50.568 -07 000000478 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:08:50.568 -07 000000512 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 192 ms to get response, and 202 ms overall 17-03-18 00:08:50.568 -07 000000610 INF: ------------ Scan Snapping tile 11 at Y-position 68.205078125, Z-position -0.174408794032621. ---------- 17-03-18 00:08:50.568 -07 000000610 INF: Cycle 82 Tile 11 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:50.568 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36628, sync = True, waitForSettling = True) 17-03-18 00:08:50.578 -07 000000610 INF: Z Motor: Moving to -0.167672 mm, started at -0.174409 mm, distance = 6.74 um 17-03-18 00:08:50.578 -07 000000610 INF: FPGA: Xmit ZDACW 36628\n [Z Motor] 17-03-18 00:08:50.578 -07 000000547 INF: FPGA: Recv ZDACW 17-03-18 00:08:50.588 -07 000000610 INF: FPGA: 'ZDACW 36628' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.588 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:50.588 -07 000000608 INF: FPGA: Recv ZDACR 36628 17-03-18 00:08:50.598 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.598 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:50.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.748 -07 000000476 INF: FPGA: Recv ZSGSRD 35386 17-03-18 00:08:50.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:50.758 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.758 -07 000000486 INF: FPGA: Recv ZSGSRD 35392 17-03-18 00:08:50.768 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.768 -07 000000547 INF: FPGA: Recv ZSGSRD 35395 17-03-18 00:08:50.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.778 -07 000000012 INF: FPGA: Recv ZSGSRD 35386 17-03-18 00:08:50.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.788 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.788 -07 000000608 INF: FPGA: Recv ZSGSRD 35395 17-03-18 00:08:50.798 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.798 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.798 -07 000000012 INF: FPGA: Recv ZSGSRD 35393 17-03-18 00:08:50.808 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.818 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.818 -07 000000012 INF: FPGA: Recv ZSGSRD 35395 17-03-18 00:08:50.828 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:50.828 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.828 -07 000000486 INF: FPGA: Recv ZSGSRD 35390 17-03-18 00:08:50.838 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:50.838 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.838 -07 000000512 INF: FPGA: Recv ZSGSRD 35395 17-03-18 00:08:50.848 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.848 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:50.848 -07 000000547 INF: FPGA: Recv ZSGSRD 35392 17-03-18 00:08:50.858 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.858 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:50.858 -07 000000608 INF: FPGA: Recv ZPIEZORD 31037 17-03-18 00:08:50.868 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:50.868 -07 000000610 INF: Z Motor: Piezo is near 46.3 V (nominally 0 to 100 V), expect near 55.9 V, since DAC is at 5.589 V. Piezo Error = -9.6 V. 17-03-18 00:08:50.868 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -167.7 um, Read 10 ADC samples: Avg = 35392 (-166.8 uncal um), Error = 0.9 uncal um, Median = 35392 (-166.8 uncal um), StdDev = 4 (0.0 uncal um), Range = 9 (0.1 uncal um), Min = 35386 (-166.8 uncal um), Max = 35395 (-166.8 uncal um), Pos Noise = 3 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:08:50.868 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35392, posNoise = 3, negNoise = 6 17-03-18 00:08:50.868 -07 000000610 INF: Z Motor: After move to -0.167672, error = -0.000017, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:50.868 -07 000000610 INF: Z Motor: MoveAbsStepped took 291 ms) 17-03-18 00:08:50.868 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:50.868 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:50.868 -07 000000012 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:50.868 -07 000000547 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:50.868 -07 000000476 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:50.868 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:50.868 -07 000000608 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:50.868 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:50.868 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:50.868 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:50.868 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:08:50.868 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:50.868 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:08:50.868 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:50.878 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:08:50.878 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:50.888 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 14 ms. 17-03-18 00:08:50.888 -07 000000476 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 19 ms. 17-03-18 00:08:50.888 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 14 ms. 17-03-18 00:08:50.888 -07 000000608 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:08:50.888 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36805 150\n [Camera] 17-03-18 00:08:51.088 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:51.438 -07 000000012 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:08:52.128 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:52.128 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:52.128 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:52.128 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:52.128 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 2317127784, 0x0, 391868352) start. 17-03-18 00:08:52.128 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:52.128 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:52.138 -07 000000512 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:52.138 -07 000000512 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:52.138 -07 000000512 INF: Camera: sBCa_ReadImage(785074704, 2352550048, 0x0, 418435064) start. 17-03-18 00:08:52.138 -07 000000486 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:52.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:52.318 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36805 1422 17-03-18 00:08:52.468 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:52.528 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 2317127784, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:08:52.528 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:08:52.528 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:52.538 -07 000000512 INF: Camera: sBCa_ReadImage(785074704, 2352550048, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:08:52.538 -07 000000512 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:08:52.538 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:52.538 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:52.538 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:08:52.538 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:52.548 -07 000000512 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:08:52.968 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:52.968 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:52.978 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:52.978 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:52.978 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:52.978 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2415923248, 0x0, 391868352) start. 17-03-18 00:08:52.978 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:52.978 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:52.978 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:52.978 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 2451345512, 0x0, 418435064) start. 17-03-18 00:08:53.168 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:08:53.168 -07 000000012 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:53.168 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36805 150' command took 2 ms to send, 2261 ms to get response, and 2272 ms overall 17-03-18 00:08:53.298 -07 000000610 INF: Skipping AutoFocus in Tile 12: adopting Best-Z from the cache: -0.168203 17-03-18 00:08:53.298 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 69.0426 mm, moving Z to -0.16820 mm 17-03-18 00:08:53.298 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:53.298 -07 000000476 INF: Y Motor: Entering MoveAbsStepped (target = 110468 steps, sync = True, waitForSettling = True) 17-03-18 00:08:53.298 -07 000000476 INF: Y Motor: Moving to 69.0426 mm, started at 68.2051 mm, delta = 0.8375 mm 17-03-18 00:08:53.298 -07 000000476 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:53.298 -07 000000012 INF: Z Motor: Entering MoveAbsStepped (DAC = 36744, sync = True, waitForSettling = False) 17-03-18 00:08:53.298 -07 000000012 INF: Z Motor: Moving to -0.168203 mm, started at -0.167689 mm, distance = -0.51 um 17-03-18 00:08:53.298 -07 000000382 INF: FPGA: Recv YRDPOS 139685 17-03-18 00:08:53.308 -07 000000476 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:08:53.308 -07 000000476 INF: Y Motor: Encoder = 139685 = motor step 109128.9 = 68.2056 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:53.308 -07 000000012 INF: FPGA: Xmit ZDACW 36744\n [Z Motor] 17-03-18 00:08:53.308 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:08:53.308 -07 000000476 INF: Y Motor: Last commanded position = 68.2126 mm (109140.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:53.308 -07 000000476 INF: Y Motor: Moving 1339 steps-15 17-03-18 00:08:53.318 -07 000000012 INF: FPGA: 'ZDACW 36744' command took 0 ms to send, 0 ms to get response, and 13 ms overall 17-03-18 00:08:53.318 -07 000000012 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:53.318 -07 000000478 INF: FPGA: Recv ZDACR 36744 17-03-18 00:08:53.328 -07 000000476 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:53.328 -07 000000012 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:53.328 -07 000000012 INF: Z Motor: MoveAbsStepped took 24 ms (did NOT wait for settling yet)) 17-03-18 00:08:53.388 -07 000000486 INF: Camera: sBCa_ReadImage(785074704, 2451345512, 0x0, 418435064) end. Duration was 407 ms. 17-03-18 00:08:53.388 -07 000000486 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 407 ms. 17-03-18 00:08:53.388 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:53.388 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:53.388 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2415923248, 0x0, 391868352) end. Duration was 414 ms. 17-03-18 00:08:53.388 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 415 ms. 17-03-18 00:08:53.388 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:53.398 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:53.398 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 421 ms. 17-03-18 00:08:53.398 -07 000000486 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 418 ms. 17-03-18 00:08:53.698 -07 000000382 INF: FPGA: Recv YMVTILE 17-03-18 00:08:53.698 -07 000000476 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 394 ms overall 17-03-18 00:08:53.748 -07 000000476 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:53.748 -07 000000512 INF: FPGA: Recv YRDPOS 141367 17-03-18 00:08:53.758 -07 000000476 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:53.758 -07 000000476 INF: Y Motor: Encoder = 141367 = motor step 110443.0 = 69.0269 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:53.758 -07 000000476 INF: Y Motor: Position error before settling (table move) = -32 encoder counts (-0.016 mm) 17-03-18 00:08:53.758 -07 000000476 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 394 ms, distance = 0.838 mm, average velocity = 2.126 mm/sec 17-03-18 00:08:54.708 -07 000000476 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:54.708 -07 000000478 INF: FPGA: Recv YRDPOS 141367 17-03-18 00:08:54.718 -07 000000476 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:54.718 -07 000000476 INF: Y Motor: Encoder = 141367 = motor step 110443.0 = 69.0269 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:54.738 -07 000000476 INF: Y Motor: After move to 69.042610, error = -0.015755, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:54.738 -07 000000476 INF: Y Motor: Position error after settling (table move) = -32 encoder counts (-0.016 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:54.738 -07 000000476 INF: Y Motor: Moved to 69.0269 after 1442 ms (table move) 17-03-18 00:08:54.748 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.168203 mm (DAC = 36744) 17-03-18 00:08:54.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.748 -07 000000512 INF: FPGA: Recv ZSGSRD 35472 17-03-18 00:08:54.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:08:54.758 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.758 -07 000000476 INF: FPGA: Recv ZSGSRD 35476 17-03-18 00:08:54.768 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.768 -07 000000382 INF: FPGA: Recv ZSGSRD 35477 17-03-18 00:08:54.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.778 -07 000000478 INF: FPGA: Recv ZSGSRD 35477 17-03-18 00:08:54.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.788 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.788 -07 000000486 INF: FPGA: Recv ZSGSRD 35470 17-03-18 00:08:54.798 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.798 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.798 -07 000000478 INF: FPGA: Recv ZSGSRD 35476 17-03-18 00:08:54.808 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.808 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.808 -07 000000486 INF: FPGA: Recv ZSGSRD 35470 17-03-18 00:08:54.818 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.818 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.818 -07 000000512 INF: FPGA: Recv ZSGSRD 35470 17-03-18 00:08:54.828 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.828 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.828 -07 000000382 INF: FPGA: Recv ZSGSRD 35474 17-03-18 00:08:54.838 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.838 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:54.838 -07 000000512 INF: FPGA: Recv ZSGSRD 35476 17-03-18 00:08:54.848 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.848 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:54.848 -07 000000486 INF: FPGA: Recv ZPIEZORD 31170 17-03-18 00:08:54.858 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.858 -07 000000610 INF: Z Motor: Piezo is near 46.6 V (nominally 0 to 100 V), expect near 56.1 V, since DAC is at 5.607 V. Piezo Error = -9.5 V. 17-03-18 00:08:54.858 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -168.2 um, Read 10 ADC samples: Avg = 35474 (-167.3 uncal um), Error = 0.9 uncal um, Median = 35475 (-167.4 uncal um), StdDev = 3 (0.0 uncal um), Range = 7 (0.0 uncal um), Min = 35470 (-167.3 uncal um), Max = 35477 (-167.4 uncal um), Pos Noise = 3 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:08:54.858 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35474, posNoise = 3, negNoise = 4 17-03-18 00:08:54.858 -07 000000610 INF: Z Motor: After move to -0.168203, error = -0.000013, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:54.858 -07 000000610 INF: Z Motor: Settle wait time = 1444 ms, measure time = 110 ms (allowed 150 ms), total time = 1554 ms 17-03-18 00:08:54.858 -07 000000610 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:54.898 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1596 ms 17-03-18 00:08:54.898 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:54.898 -07 000000610 INF: ------------ Scan Snapping tile 12 at Y-position 69.02685546875, Z-position -0.168215853527963. ---------- 17-03-18 00:08:54.898 -07 000000610 INF: Cycle 82 Tile 12 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:54.898 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36567, sync = True, waitForSettling = True) 17-03-18 00:08:54.898 -07 000000610 INF: Z Motor: Moving to -0.167393 mm, started at -0.168216 mm, distance = 0.82 um 17-03-18 00:08:54.898 -07 000000610 INF: FPGA: Xmit ZDACW 36567\n [Z Motor] 17-03-18 00:08:54.898 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:08:54.908 -07 000000610 INF: FPGA: 'ZDACW 36567' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:08:54.908 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:54.908 -07 000000486 INF: FPGA: Recv ZDACR 36567 17-03-18 00:08:54.918 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:54.918 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:55.068 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.068 -07 000000382 INF: FPGA: Recv ZSGSRD 35347 17-03-18 00:08:55.078 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:55.078 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.078 -07 000000486 INF: FPGA: Recv ZSGSRD 35351 17-03-18 00:08:55.088 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.088 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.088 -07 000000382 INF: FPGA: Recv ZSGSRD 35344 17-03-18 00:08:55.098 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.098 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.098 -07 000000476 INF: FPGA: Recv ZSGSRD 35349 17-03-18 00:08:55.108 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.108 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.108 -07 000000012 INF: FPGA: Recv ZSGSRD 35350 17-03-18 00:08:55.118 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.118 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.118 -07 000000512 INF: FPGA: Recv ZSGSRD 35345 17-03-18 00:08:55.128 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.128 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.128 -07 000000012 INF: FPGA: Recv ZSGSRD 35339 17-03-18 00:08:55.138 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.138 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.138 -07 000000382 INF: FPGA: Recv ZSGSRD 35344 17-03-18 00:08:55.148 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.148 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.148 -07 000000512 INF: FPGA: Recv ZSGSRD 35349 17-03-18 00:08:55.158 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.158 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:55.158 -07 000000486 INF: FPGA: Recv ZSGSRD 35347 17-03-18 00:08:55.168 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.168 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:55.168 -07 000000512 INF: FPGA: Recv ZPIEZORD 31007 17-03-18 00:08:55.178 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:55.178 -07 000000610 INF: Z Motor: Piezo is near 46.2 V (nominally 0 to 100 V), expect near 55.8 V, since DAC is at 5.580 V. Piezo Error = -9.6 V. 17-03-18 00:08:55.178 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -167.4 um, Read 10 ADC samples: Avg = 35346 (-166.5 uncal um), Error = 0.9 uncal um, Median = 35347 (-166.5 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 35339 (-166.5 uncal um), Max = 35351 (-166.6 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:08:55.178 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35346, posNoise = 5, negNoise = 7 17-03-18 00:08:55.178 -07 000000610 INF: Z Motor: After move to -0.167393, error = -0.000001, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:55.178 -07 000000610 INF: Z Motor: MoveAbsStepped took 274 ms) 17-03-18 00:08:55.178 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:55.178 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:55.178 -07 000000012 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:55.178 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:55.178 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:55.178 -07 000000382 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:55.178 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:55.178 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:08:55.178 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:55.178 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:55.178 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 4 ms. 17-03-18 00:08:55.178 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:55.188 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 6 ms. 17-03-18 00:08:55.188 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:55.188 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:08:55.188 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:55.198 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:08:55.198 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 24 ms. 17-03-18 00:08:55.198 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:08:55.198 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 23 ms. 17-03-18 00:08:55.208 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36744 150\n [Camera] 17-03-18 00:08:55.408 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:08:56.438 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:08:56.438 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:08:56.438 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:56.438 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:56.438 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:08:56.448 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:08:56.448 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:08:56.448 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:56.448 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:56.448 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:08:56.638 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36744 1422 17-03-18 00:08:56.788 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:08:56.808 -07 000000627 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:08:56.838 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 398 ms. 17-03-18 00:08:56.838 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 396 ms. 17-03-18 00:08:56.838 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 396 ms. 17-03-18 00:08:56.838 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:56.838 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 398 ms. 17-03-18 00:08:56.838 -07 000000586 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:56.848 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 6 ms. 17-03-18 00:08:56.848 -07 000000586 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:08:56.848 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 406 ms. 17-03-18 00:08:56.858 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:08:57.138 -07 000000627 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:08:57.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:08:57.288 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:08:57.288 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:08:57.288 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:08:57.288 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:08:57.288 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) start. 17-03-18 00:08:57.288 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:08:57.288 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:08:57.288 -07 000000476 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:08:57.288 -07 000000476 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:08:57.288 -07 000000476 INF: Camera: sBCa_ReadImage(785074704, 2486767776, 0x0, 418435064) start. 17-03-18 00:08:57.478 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP 2264 17-03-18 00:08:57.478 -07 000000486 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:08:57.478 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36744 150' command took 2 ms to send, 2262 ms to get response, and 2272 ms overall 17-03-18 00:08:57.478 -07 000000610 INF: Skipping AutoFocus in Tile 13: adopting Best-Z from the cache: -0.167815 17-03-18 00:08:57.478 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 69.8726 mm, moving Z to -0.16781 mm 17-03-18 00:08:57.478 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:08:57.478 -07 000000482 INF: Y Motor: Entering MoveAbsStepped (target = 111796 steps, sync = True, waitForSettling = True) 17-03-18 00:08:57.478 -07 000000482 INF: Y Motor: Moving to 69.8726 mm, started at 69.0269 mm, delta = 0.8458 mm 17-03-18 00:08:57.478 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:57.478 -07 000000382 INF: Z Motor: Entering MoveAbsStepped (DAC = 36659, sync = True, waitForSettling = False) 17-03-18 00:08:57.478 -07 000000382 INF: Z Motor: Moving to -0.167814 mm, started at -0.167394 mm, distance = -0.42 um 17-03-18 00:08:57.488 -07 000000586 INF: FPGA: Recv YRDPOS 141367 17-03-18 00:08:57.488 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 2 ms overall 17-03-18 00:08:57.488 -07 000000482 INF: Y Motor: Encoder = 141367 = motor step 110443.0 = 69.0269 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:57.488 -07 000000482 INF: Y Motor: Last commanded position = 69.0426 mm (110468.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:08:57.488 -07 000000482 INF: Y Motor: Moving 1353 steps-32 17-03-18 00:08:57.488 -07 000000382 INF: FPGA: Xmit ZDACW 36659\n [Z Motor] 17-03-18 00:08:57.488 -07 000000586 INF: FPGA: Recv ZDACW 17-03-18 00:08:57.498 -07 000000382 INF: FPGA: 'ZDACW 36659' command took 1 ms to send, 0 ms to get response, and 11 ms overall 17-03-18 00:08:57.498 -07 000000482 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:08:57.508 -07 000000382 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:57.508 -07 000000512 INF: FPGA: Recv ZDACR 36659 17-03-18 00:08:57.518 -07 000000382 INF: FPGA: 'ZDACR' command took 1 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:08:57.518 -07 000000382 INF: Z Motor: MoveAbsStepped took 31 ms (did NOT wait for settling yet)) 17-03-18 00:08:57.688 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) end. Duration was 403 ms. 17-03-18 00:08:57.688 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:08:57.688 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:57.688 -07 000000476 INF: Camera: sBCa_ReadImage(785074704, 2486767776, 0x0, 418435064) end. Duration was 402 ms. 17-03-18 00:08:57.688 -07 000000476 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 403 ms. 17-03-18 00:08:57.688 -07 000000476 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:08:57.688 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:57.688 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 407 ms. 17-03-18 00:08:57.698 -07 000000476 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:08:57.698 -07 000000476 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 413 ms. 17-03-18 00:08:57.868 -07 000000627 INF: FPGA: Recv YMVTILE 17-03-18 00:08:57.868 -07 000000482 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 387 ms overall 17-03-18 00:08:57.918 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:57.918 -07 000000627 INF: FPGA: Recv YRDPOS 143079 17-03-18 00:08:57.928 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:57.928 -07 000000482 INF: Y Motor: Encoder = 143079 = motor step 111780.5 = 69.8628 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:57.928 -07 000000482 INF: Y Motor: Position error before settling (table move) = -20 encoder counts (-0.010 mm) 17-03-18 00:08:57.928 -07 000000482 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 387 ms, distance = 0.846 mm, average velocity = 2.185 mm/sec 17-03-18 00:08:58.878 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:08:58.878 -07 000000382 INF: FPGA: Recv YRDPOS 143079 17-03-18 00:08:58.888 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:58.888 -07 000000482 INF: Y Motor: Encoder = 143079 = motor step 111780.5 = 69.8628 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:08:58.908 -07 000000482 INF: Y Motor: After move to 69.872612, error = -0.009819, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:08:58.908 -07 000000482 INF: Y Motor: Position error after settling (table move) = -20 encoder counts (-0.010 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:08:58.908 -07 000000482 INF: Y Motor: Moved to 69.8628 after 1430 ms (table move) 17-03-18 00:08:58.908 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.167814 mm (DAC = 36659) 17-03-18 00:08:58.918 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.918 -07 000000478 INF: FPGA: Recv ZSGSRD 35415 17-03-18 00:08:58.928 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:58.928 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.928 -07 000000382 INF: FPGA: Recv ZSGSRD 35418 17-03-18 00:08:58.938 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:58.938 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.938 -07 000000482 INF: FPGA: Recv ZSGSRD 35415 17-03-18 00:08:58.948 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:58.948 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.948 -07 000000482 INF: FPGA: Recv ZSGSRD 35422 17-03-18 00:08:58.958 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:58.958 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.958 -07 000000012 INF: FPGA: Recv ZSGSRD 35415 17-03-18 00:08:58.968 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:58.968 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.968 -07 000000586 INF: FPGA: Recv ZSGSRD 35410 17-03-18 00:08:58.978 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:58.978 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.978 -07 000000478 INF: FPGA: Recv ZSGSRD 35410 17-03-18 00:08:58.988 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:58.988 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.988 -07 000000478 INF: FPGA: Recv ZSGSRD 35408 17-03-18 00:08:58.998 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:58.998 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:58.998 -07 000000486 INF: FPGA: Recv ZSGSRD 35413 17-03-18 00:08:59.008 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.008 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.008 -07 000000486 INF: FPGA: Recv ZSGSRD 35423 17-03-18 00:08:59.018 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.018 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:59.018 -07 000000627 INF: FPGA: Recv ZPIEZORD 31114 17-03-18 00:08:59.028 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.028 -07 000000610 INF: Z Motor: Piezo is near 46.5 V (nominally 0 to 100 V), expect near 55.9 V, since DAC is at 5.594 V. Piezo Error = -9.5 V. 17-03-18 00:08:59.028 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -167.8 um, Read 10 ADC samples: Avg = 35415 (-167.0 uncal um), Error = 0.8 uncal um, Median = 35415 (-167.0 uncal um), StdDev = 5 (0.0 uncal um), Range = 15 (0.1 uncal um), Min = 35408 (-166.9 uncal um), Max = 35423 (-167.0 uncal um), Pos Noise = 8 (0.1 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:08:59.028 -07 000000610 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35415, posNoise = 8, negNoise = 7 17-03-18 00:08:59.028 -07 000000610 INF: Z Motor: After move to -0.167814, error = -0.000023, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:59.028 -07 000000610 INF: Z Motor: Settle wait time = 1431 ms, measure time = 111 ms (allowed 150 ms), total time = 1542 ms 17-03-18 00:08:59.028 -07 000000610 INF: Z Motor: Waiting extra 39 ms after reading position to reach expected total of 150 ms 17-03-18 00:08:59.068 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1585 ms 17-03-18 00:08:59.068 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:08:59.068 -07 000000610 INF: ------------ Scan Snapping tile 13 at Y-position 69.86279296875, Z-position -0.167836825011599. ---------- 17-03-18 00:08:59.068 -07 000000610 INF: Cycle 82 Tile 13 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:08:59.068 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36482, sync = True, waitForSettling = True) 17-03-18 00:08:59.068 -07 000000610 INF: Z Motor: Moving to -0.167004 mm, started at -0.167837 mm, distance = 0.83 um 17-03-18 00:08:59.068 -07 000000610 INF: FPGA: Xmit ZDACW 36482\n [Z Motor] 17-03-18 00:08:59.068 -07 000000482 INF: FPGA: Recv ZDACW 17-03-18 00:08:59.078 -07 000000610 INF: FPGA: 'ZDACW 36482' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:08:59.078 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:08:59.078 -07 000000482 INF: FPGA: Recv ZDACR 36482 17-03-18 00:08:59.088 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.088 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:08:59.238 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.238 -07 000000482 INF: FPGA: Recv ZSGSRD 35285 17-03-18 00:08:59.248 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:08:59.248 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.248 -07 000000448 INF: FPGA: Recv ZSGSRD 35293 17-03-18 00:08:59.258 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.258 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.258 -07 000000627 INF: FPGA: Recv ZSGSRD 35290 17-03-18 00:08:59.268 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.268 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.268 -07 000000482 INF: FPGA: Recv ZSGSRD 35282 17-03-18 00:08:59.278 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.278 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.278 -07 000000627 INF: FPGA: Recv ZSGSRD 35288 17-03-18 00:08:59.288 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.288 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.288 -07 000000476 INF: FPGA: Recv ZSGSRD 35284 17-03-18 00:08:59.298 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.298 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.298 -07 000000627 INF: FPGA: Recv ZSGSRD 35285 17-03-18 00:08:59.308 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.308 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.308 -07 000000482 INF: FPGA: Recv ZSGSRD 35287 17-03-18 00:08:59.318 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.318 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.318 -07 000000627 INF: FPGA: Recv ZSGSRD 35289 17-03-18 00:08:59.328 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.328 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:08:59.328 -07 000000482 INF: FPGA: Recv ZSGSRD 35292 17-03-18 00:08:59.338 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.338 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:08:59.338 -07 000000627 INF: FPGA: Recv ZPIEZORD 30946 17-03-18 00:08:59.348 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:08:59.348 -07 000000610 INF: Z Motor: Piezo is near 46.1 V (nominally 0 to 100 V), expect near 55.7 V, since DAC is at 5.567 V. Piezo Error = -9.6 V. 17-03-18 00:08:59.348 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -167.0 um, Read 10 ADC samples: Avg = 35288 (-166.2 uncal um), Error = 0.9 uncal um, Median = 35288 (-166.2 uncal um), StdDev = 4 (0.0 uncal um), Range = 11 (0.1 uncal um), Min = 35282 (-166.1 uncal um), Max = 35293 (-166.2 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:08:59.348 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35288, posNoise = 5, negNoise = 6 17-03-18 00:08:59.348 -07 000000610 INF: Z Motor: After move to -0.167004, error = -0.000017, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:08:59.348 -07 000000610 INF: Z Motor: MoveAbsStepped took 277 ms) 17-03-18 00:08:59.348 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:08:59.348 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:08:59.348 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:08:59.348 -07 000000476 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:08:59.348 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:08:59.348 -07 000000448 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:08:59.348 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:08:59.348 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:08:59.348 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:08:59.348 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:08:59.348 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:08:59.348 -07 000000486 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:08:59.358 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:08:59.358 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:08:59.358 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 2 ms. 17-03-18 00:08:59.358 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:08:59.368 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 9 ms. 17-03-18 00:08:59.368 -07 000000476 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 14 ms. 17-03-18 00:08:59.368 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 10 ms. 17-03-18 00:08:59.368 -07 000000448 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 14 ms. 17-03-18 00:08:59.368 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36659 150\n [Camera] 17-03-18 00:08:59.568 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:09:00.608 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:00.608 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:00.608 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:00.608 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:00.608 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) start. 17-03-18 00:09:00.608 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:00.608 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:00.608 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:00.728 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:00.728 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:00.798 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36659 1422 17-03-18 00:09:00.948 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:00.998 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) end. Duration was 393 ms. 17-03-18 00:09:00.998 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:09:00.998 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:00.998 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:00.998 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 397 ms. 17-03-18 00:09:01.118 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 394 ms. 17-03-18 00:09:01.118 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 394 ms. 17-03-18 00:09:01.118 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:01.118 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 2 ms. 17-03-18 00:09:01.128 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 519 ms. 17-03-18 00:09:01.448 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:01.448 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:01.448 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:01.448 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:01.448 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) start. 17-03-18 00:09:01.448 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:01.448 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:01.448 -07 000000476 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:01.448 -07 000000476 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:01.448 -07 000000476 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) start. 17-03-18 00:09:01.638 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:01.638 -07 000000382 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:01.638 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36659 150' command took 2 ms to send, 2265 ms to get response, and 2272 ms overall 17-03-18 00:09:01.638 -07 000000610 INF: Skipping AutoFocus in Tile 14: adopting Best-Z from the cache: -0.167092 17-03-18 00:09:01.638 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 70.7026 mm, moving Z to -0.16709 mm 17-03-18 00:09:01.638 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:01.638 -07 000000486 INF: Y Motor: Entering MoveAbsStepped (target = 113124 steps, sync = True, waitForSettling = True) 17-03-18 00:09:01.638 -07 000000486 INF: Y Motor: Moving to 70.7026 mm, started at 69.8628 mm, delta = 0.8398 mm 17-03-18 00:09:01.638 -07 000000382 INF: Z Motor: Entering MoveAbsStepped (DAC = 36501, sync = True, waitForSettling = False) 17-03-18 00:09:01.648 -07 000000382 INF: Z Motor: Moving to -0.167091 mm, started at -0.167021 mm, distance = -0.07 um 17-03-18 00:09:01.648 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:01.648 -07 000000482 INF: FPGA: Recv YRDPOS 143080 17-03-18 00:09:01.658 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:01.658 -07 000000382 INF: FPGA: Xmit ZDACW 36501\n [Z Motor] 17-03-18 00:09:01.658 -07 000000486 INF: Y Motor: Encoder = 143080 = motor step 111781.3 = 69.8633 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:01.658 -07 000000486 INF: Y Motor: Last commanded position = 69.8726 mm (111796.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:01.658 -07 000000486 INF: Y Motor: Moving 1343 steps-20 17-03-18 00:09:01.658 -07 000000448 INF: FPGA: Recv ZDACW 17-03-18 00:09:01.668 -07 000000382 INF: FPGA: 'ZDACW 36501' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:01.668 -07 000000382 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:01.668 -07 000000448 INF: FPGA: Recv ZDACR 36501 17-03-18 00:09:01.678 -07 000000382 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:01.678 -07 000000382 INF: Z Motor: MoveAbsStepped took 30 ms (did NOT wait for settling yet)) 17-03-18 00:09:01.678 -07 000000486 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:01.848 -07 000000476 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) end. Duration was 396 ms. 17-03-18 00:09:01.848 -07 000000476 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 396 ms. 17-03-18 00:09:01.848 -07 000000476 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:01.848 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) end. Duration was 400 ms. 17-03-18 00:09:01.848 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 401 ms. 17-03-18 00:09:01.848 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:01.848 -07 000000476 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:01.848 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:01.858 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 405 ms. 17-03-18 00:09:01.858 -07 000000476 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 408 ms. 17-03-18 00:09:02.048 -07 000000382 INF: FPGA: Recv YMVTILE 17-03-18 00:09:02.048 -07 000000486 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 394 ms overall 17-03-18 00:09:02.098 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:02.098 -07 000000547 INF: FPGA: Recv YRDPOS 144769 17-03-18 00:09:02.108 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:02.108 -07 000000486 INF: Y Motor: Encoder = 144769 = motor step 113100.8 = 70.6880 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:02.108 -07 000000486 INF: Y Motor: Position error before settling (table move) = -30 encoder counts (-0.015 mm) 17-03-18 00:09:02.108 -07 000000486 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 394 ms, distance = 0.840 mm, average velocity = 2.132 mm/sec 17-03-18 00:09:02.138 -07 000000448 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:02.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:02.178 -07 000000476 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:09:03.058 -07 000000486 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:03.058 -07 000000448 INF: FPGA: Recv YRDPOS 144769 17-03-18 00:09:03.068 -07 000000486 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 11 ms overall 17-03-18 00:09:03.068 -07 000000486 INF: Y Motor: Encoder = 144769 = motor step 113100.8 = 70.6880 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:03.088 -07 000000486 INF: Y Motor: After move to 70.702606, error = -0.014618, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:03.088 -07 000000486 INF: Y Motor: Position error after settling (table move) = -30 encoder counts (-0.015 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:03.088 -07 000000486 INF: Y Motor: Moved to 70.6880 after 1448 ms (table move) 17-03-18 00:09:03.098 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.167091 mm (DAC = 36501) 17-03-18 00:09:03.098 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.098 -07 000000627 INF: FPGA: Recv ZSGSRD 35300 17-03-18 00:09:03.108 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:03.108 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.108 -07 000000476 INF: FPGA: Recv ZSGSRD 35304 17-03-18 00:09:03.118 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.118 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.118 -07 000000476 INF: FPGA: Recv ZSGSRD 35302 17-03-18 00:09:03.128 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.128 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.128 -07 000000448 INF: FPGA: Recv ZSGSRD 35304 17-03-18 00:09:03.138 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.138 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.138 -07 000000486 INF: FPGA: Recv ZSGSRD 35305 17-03-18 00:09:03.148 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.148 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.148 -07 000000486 INF: FPGA: Recv ZSGSRD 35302 17-03-18 00:09:03.158 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.158 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.158 -07 000000586 INF: FPGA: Recv ZSGSRD 35299 17-03-18 00:09:03.168 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.168 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.168 -07 000000486 INF: FPGA: Recv ZSGSRD 35298 17-03-18 00:09:03.178 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.178 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.178 -07 000000448 INF: FPGA: Recv ZSGSRD 35305 17-03-18 00:09:03.188 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.188 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.188 -07 000000486 INF: FPGA: Recv ZSGSRD 35301 17-03-18 00:09:03.198 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.198 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:03.198 -07 000000448 INF: FPGA: Recv ZPIEZORD 30975 17-03-18 00:09:03.208 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.208 -07 000000610 INF: Z Motor: Piezo is near 46.2 V (nominally 0 to 100 V), expect near 55.7 V, since DAC is at 5.570 V. Piezo Error = -9.5 V. 17-03-18 00:09:03.208 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -167.1 um, Read 10 ADC samples: Avg = 35302 (-166.2 uncal um), Error = 0.8 uncal um, Median = 35302 (-166.2 uncal um), StdDev = 2 (0.0 uncal um), Range = 7 (0.0 uncal um), Min = 35298 (-166.2 uncal um), Max = 35305 (-166.3 uncal um), Pos Noise = 3 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:09:03.208 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35302, posNoise = 3, negNoise = 4 17-03-18 00:09:03.208 -07 000000610 INF: Z Motor: After move to -0.167091, error = -0.000020, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:03.208 -07 000000610 INF: Z Motor: Settle wait time = 1451 ms, measure time = 109 ms (allowed 150 ms), total time = 1560 ms 17-03-18 00:09:03.208 -07 000000610 INF: Z Motor: Waiting extra 41 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:03.258 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1612 ms 17-03-18 00:09:03.258 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:03.258 -07 000000610 INF: ------------ Scan Snapping tile 14 at Y-position 70.68798828125, Z-position -0.16711088903958. ---------- 17-03-18 00:09:03.258 -07 000000610 INF: Cycle 82 Tile 14 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:03.258 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36325, sync = True, waitForSettling = True) 17-03-18 00:09:03.258 -07 000000610 INF: Z Motor: Moving to -0.166285 mm, started at -0.167111 mm, distance = 0.83 um 17-03-18 00:09:03.258 -07 000000610 INF: FPGA: Xmit ZDACW 36325\n [Z Motor] 17-03-18 00:09:03.258 -07 000000586 INF: FPGA: Recv ZDACW 17-03-18 00:09:03.268 -07 000000610 INF: FPGA: 'ZDACW 36325' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:09:03.268 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:03.268 -07 000000476 INF: FPGA: Recv ZDACR 36325 17-03-18 00:09:03.278 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.278 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:03.428 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.428 -07 000000482 INF: FPGA: Recv ZSGSRD 35174 17-03-18 00:09:03.438 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:03.438 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.438 -07 000000382 INF: FPGA: Recv ZSGSRD 35179 17-03-18 00:09:03.448 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:03.448 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.448 -07 000000627 INF: FPGA: Recv ZSGSRD 35180 17-03-18 00:09:03.458 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.458 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.458 -07 000000608 INF: FPGA: Recv ZSGSRD 35175 17-03-18 00:09:03.468 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.468 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.468 -07 000000382 INF: FPGA: Recv ZSGSRD 35177 17-03-18 00:09:03.478 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.478 -07 000000486 INF: FPGA: Recv ZSGSRD 35170 17-03-18 00:09:03.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.488 -07 000000486 INF: FPGA: Recv ZSGSRD 35180 17-03-18 00:09:03.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.498 -07 000000482 INF: FPGA: Recv ZSGSRD 35177 17-03-18 00:09:03.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.508 -07 000000627 INF: FPGA: Recv ZSGSRD 35171 17-03-18 00:09:03.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:03.518 -07 000000608 INF: FPGA: Recv ZSGSRD 35177 17-03-18 00:09:03.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.528 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:03.528 -07 000000476 INF: FPGA: Recv ZPIEZORD 30805 17-03-18 00:09:03.538 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:03.538 -07 000000610 INF: Z Motor: Piezo is near 45.8 V (nominally 0 to 100 V), expect near 55.4 V, since DAC is at 5.543 V. Piezo Error = -9.6 V. 17-03-18 00:09:03.538 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -166.3 um, Read 10 ADC samples: Avg = 35176 (-165.4 uncal um), Error = 0.8 uncal um, Median = 35177 (-165.4 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 35170 (-165.4 uncal um), Max = 35180 (-165.5 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:09:03.538 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35176, posNoise = 4, negNoise = 6 17-03-18 00:09:03.538 -07 000000610 INF: Z Motor: After move to -0.166285, error = -0.000016, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:03.538 -07 000000610 INF: Z Motor: MoveAbsStepped took 276 ms) 17-03-18 00:09:03.538 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:03.538 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:03.538 -07 000000382 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:03.538 -07 000000547 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:03.538 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:03.538 -07 000000608 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:03.538 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:03.538 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:03.538 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:09:03.538 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:03.538 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:09:03.538 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:03.538 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:03.538 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:03.538 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 2 ms. 17-03-18 00:09:03.538 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:03.548 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 8 ms. 17-03-18 00:09:03.548 -07 000000547 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 11 ms. 17-03-18 00:09:03.548 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 9 ms. 17-03-18 00:09:03.548 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 13 ms. 17-03-18 00:09:03.558 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36501 150\n [Camera] 17-03-18 00:09:03.768 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP turning red on 199 17-03-18 00:09:04.798 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:04.798 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:04.798 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:04.798 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:04.798 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) start. 17-03-18 00:09:04.798 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:04.798 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:04.798 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:04.798 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:04.798 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) start. 17-03-18 00:09:04.988 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36501 1422 17-03-18 00:09:05.138 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:05.198 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) end. Duration was 398 ms. 17-03-18 00:09:05.198 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 398 ms. 17-03-18 00:09:05.198 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:05.198 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) end. Duration was 397 ms. 17-03-18 00:09:05.198 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 397 ms. 17-03-18 00:09:05.198 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:05.198 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:05.198 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:09:05.198 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:05.208 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 408 ms. 17-03-18 00:09:05.638 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:05.638 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:05.638 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:05.638 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:05.638 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) start. 17-03-18 00:09:05.638 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:05.638 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:05.638 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:05.758 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:05.758 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:05.828 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:05.828 -07 000000476 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:05.828 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36501 150' command took 2 ms to send, 2266 ms to get response, and 2275 ms overall 17-03-18 00:09:05.828 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 0 - Best Z for this model is: -0.172880 17-03-18 00:09:05.828 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 15 at Y-position 70.68798828125, Z-Position -0.166301438309718. ------ 17-03-18 00:09:05.838 -07 000000627 INF: Y Motor: MoveYZ optimized starting. Moving Y to 71.5326 mm, moving Z to -0.17273 mm 17-03-18 00:09:05.838 -07 000000627 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:05.838 -07 000000547 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:05.838 -07 000000586 INF: Z Motor: Entering MoveAbsStepped (DAC = 37734, sync = True, waitForSettling = False) 17-03-18 00:09:05.838 -07 000000586 INF: Z Motor: Moving to -0.172735 mm, started at -0.166301 mm, distance = -6.43 um 17-03-18 00:09:05.838 -07 000000482 INF: Y Motor: Entering MoveAbsStepped (target = 114452 steps, sync = True, waitForSettling = True) 17-03-18 00:09:05.838 -07 000000482 INF: Y Motor: Moving to 71.5326 mm, started at 70.6880 mm, delta = 0.8446 mm 17-03-18 00:09:05.838 -07 000000512 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:05.848 -07 000000547 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:05.848 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:05.858 -07 000000586 INF: FPGA: Xmit ZDACW 37734\n [Z Motor] 17-03-18 00:09:05.868 -07 000000547 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:09:06.038 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) end. Duration was 400 ms. 17-03-18 00:09:06.038 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:09:06.038 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:06.038 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:06.038 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 404 ms. 17-03-18 00:09:06.058 -07 000000486 INF: FPGA: Recv YRDPOS 144770 17-03-18 00:09:06.058 -07 000000486 INF: FPGA: Recv ZDACW 17-03-18 00:09:06.058 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 200 ms to get response, and 214 ms overall 17-03-18 00:09:06.058 -07 000000482 INF: Y Motor: Encoder = 144770 = motor step 113101.6 = 70.6885 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:06.058 -07 000000586 INF: FPGA: 'ZDACW 37734' command took 0 ms to send, 190 ms to get response, and 214 ms overall 17-03-18 00:09:06.058 -07 000000586 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:06.058 -07 000000482 INF: Y Motor: Last commanded position = 70.7026 mm (113124.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:06.058 -07 000000482 INF: Y Motor: Moving 1351 steps-30 17-03-18 00:09:06.068 -07 000000486 INF: FPGA: Recv ZDACR 37734 17-03-18 00:09:06.068 -07 000000586 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:06.068 -07 000000586 INF: Z Motor: MoveAbsStepped took 224 ms (did NOT wait for settling yet)) 17-03-18 00:09:06.068 -07 000000482 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:06.068 -07 000000486 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:09:06.068 -07 000000547 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 192 ms to get response, and 222 ms overall 17-03-18 00:09:06.158 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 396 ms. 17-03-18 00:09:06.158 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 396 ms. 17-03-18 00:09:06.158 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:06.158 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 2 ms. 17-03-18 00:09:06.158 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 521 ms. 17-03-18 00:09:06.438 -07 000000547 INF: FPGA: Recv YMVTILE 17-03-18 00:09:06.438 -07 000000482 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 383 ms overall 17-03-18 00:09:06.478 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:06.488 -07 000000586 INF: FPGA: Recv YRDPOS 146470 17-03-18 00:09:06.498 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:06.498 -07 000000482 INF: Y Motor: Encoder = 146470 = motor step 114429.7 = 71.5186 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:06.498 -07 000000482 INF: Y Motor: Position error before settling (table move) = -29 encoder counts (-0.014 mm) 17-03-18 00:09:06.498 -07 000000482 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 383 ms, distance = 0.845 mm, average velocity = 2.205 mm/sec 17-03-18 00:09:07.138 -07 000000586 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:07.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:07.448 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:07.448 -07 000000382 INF: FPGA: Recv YRDPOS 146470 17-03-18 00:09:07.458 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:07.458 -07 000000482 INF: Y Motor: Encoder = 146470 = motor step 114429.7 = 71.5186 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:07.478 -07 000000482 INF: Y Motor: After move to 71.532608, error = -0.014053, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:07.478 -07 000000482 INF: Y Motor: Position error after settling (table move) = -29 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:07.478 -07 000000482 INF: Y Motor: Moved to 71.5186 after 1640 ms (table move) 17-03-18 00:09:07.478 -07 000000627 INF: Z Motor: EndOfMoveAbsStepped: target = -0.172735 mm (DAC = 37734) 17-03-18 00:09:07.478 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.488 -07 000000482 INF: FPGA: Recv ZSGSRD 36185 17-03-18 00:09:07.498 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:07.498 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.498 -07 000000486 INF: FPGA: Recv ZSGSRD 36178 17-03-18 00:09:07.508 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:07.508 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.508 -07 000000486 INF: FPGA: Recv ZSGSRD 36186 17-03-18 00:09:07.518 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:07.518 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.518 -07 000000482 INF: FPGA: Recv ZSGSRD 36182 17-03-18 00:09:07.528 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:07.528 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.528 -07 000000586 INF: FPGA: Recv ZSGSRD 36182 17-03-18 00:09:07.538 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:07.538 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.538 -07 000000547 INF: FPGA: Recv ZSGSRD 36187 17-03-18 00:09:07.548 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:07.558 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.558 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:09:07.558 -07 000000486 INF: FPGA: Recv ZSGSRD 36181 17-03-18 00:09:07.568 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:07.568 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.568 -07 000000482 INF: FPGA: Recv ZSGSRD 36171 17-03-18 00:09:07.578 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:07.588 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.588 -07 000000382 INF: FPGA: Recv ZSGSRD 36188 17-03-18 00:09:07.598 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:07.598 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:07.598 -07 000000382 INF: FPGA: Recv ZSGSRD 36189 17-03-18 00:09:07.608 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:07.628 -07 000000627 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:07.628 -07 000000382 INF: FPGA: Recv ZPIEZORD 32170 17-03-18 00:09:07.638 -07 000000627 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:09:07.638 -07 000000627 INF: Z Motor: Piezo is near 48.7 V (nominally 0 to 100 V), expect near 57.6 V, since DAC is at 5.758 V. Piezo Error = -8.9 V. 17-03-18 00:09:07.638 -07 000000627 INF: Z Motor: ReadAdcAverage: Commanded Z = -172.7 um, Read 10 ADC samples: Avg = 36183 (-171.9 uncal um), Error = 0.8 uncal um, Median = 36184 (-171.9 uncal um), StdDev = 5 (0.0 uncal um), Range = 18 (0.1 uncal um), Min = 36171 (-171.8 uncal um), Max = 36189 (-171.9 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 12 (0.1 uncal um) 17-03-18 00:09:07.638 -07 000000627 INF: Z Motor: ADC noise = 12, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 36183, posNoise = 6, negNoise = 12 17-03-18 00:09:07.638 -07 000000627 INF: Z Motor: After move to -0.172735, error = -0.000035, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:07.638 -07 000000627 INF: Z Motor: Settle wait time = 1644 ms, measure time = 150 ms (allowed 150 ms), total time = 1794 ms 17-03-18 00:09:07.638 -07 000000627 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1798 ms 17-03-18 00:09:07.638 -07 000000627 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:07.638 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:07.638 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:07.638 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:07.638 -07 000000547 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:07.638 -07 000000486 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:09:07.638 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:07.648 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:09:07.648 -07 000000486 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:09:07.648 -07 000000486 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:07.648 -07 000000486 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:09:07.658 -07 000000486 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 7 ms. 17-03-18 00:09:07.658 -07 000000486 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 11 ms. 17-03-18 00:09:07.658 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:09:07.708 -07 000000482 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:09:08.008 -07 000000547 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:09:08.008 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:09:08.008 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:09:08.008 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:09:08.048 -07 000000586 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:09:08.048 -07 000000482 INF: FPGA: Recv CAMSNAP 17-03-18 00:09:08.048 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 381 ms to get response, and 389 ms overall 17-03-18 00:09:08.128 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 118 ms. 17-03-18 00:09:08.128 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:08.128 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:09:08.128 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 121 ms. 17-03-18 00:09:08.148 -07 000000382 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:08.148 -07 000000486 INF: Autofocus start 17-03-18 00:09:08.148 -07 000000486 INF: MeasureProfile start 17-03-18 00:09:08.148 -07 000000586 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:08.158 -07 000000382 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:08.158 -07 000000382 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:09:08.158 -07 000000486 INF: MeasureProfile done 17-03-18 00:09:08.158 -07 000000486 INF: X=80 Z correction [um]=0.337546 17-03-18 00:09:08.158 -07 000000486 INF: Autofocus finished 17-03-18 00:09:08.368 -07 000000486 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:09:08.368 -07 000000382 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 200 ms to get response, and 209 ms overall 17-03-18 00:09:08.368 -07 000000610 INF: ------------ Scan Snapping tile 15 at Y-position 71.5185546875, Z-position -0.172770619936472. ---------- 17-03-18 00:09:08.368 -07 000000610 INF: Cycle 82 Tile 15 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:08.368 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36320, sync = True, waitForSettling = True) 17-03-18 00:09:08.368 -07 000000610 INF: Z Motor: Moving to -0.166262 mm, started at -0.172771 mm, distance = 6.51 um 17-03-18 00:09:08.368 -07 000000610 INF: FPGA: Xmit ZDACW 36320\n [Z Motor] 17-03-18 00:09:08.378 -07 000000012 INF: FPGA: Recv ZDACW 17-03-18 00:09:08.378 -07 000000610 INF: FPGA: 'ZDACW 36320' command took 0 ms to send, 1 ms to get response, and 2 ms overall 17-03-18 00:09:08.378 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:08.378 -07 000000486 INF: FPGA: Recv ZDACR 36320 17-03-18 00:09:08.388 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:08.388 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:08.538 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.538 -07 000000547 INF: FPGA: Recv ZSGSRD 35171 17-03-18 00:09:08.548 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:08.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.548 -07 000000482 INF: FPGA: Recv ZSGSRD 35173 17-03-18 00:09:08.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.558 -07 000000547 INF: FPGA: Recv ZSGSRD 35177 17-03-18 00:09:08.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.568 -07 000000486 INF: FPGA: Recv ZSGSRD 35169 17-03-18 00:09:08.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.578 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.578 -07 000000382 INF: FPGA: Recv ZSGSRD 35172 17-03-18 00:09:08.588 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.588 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.588 -07 000000586 INF: FPGA: Recv ZSGSRD 35180 17-03-18 00:09:08.598 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.598 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.598 -07 000000382 INF: FPGA: Recv ZSGSRD 35172 17-03-18 00:09:08.608 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.608 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.608 -07 000000486 INF: FPGA: Recv ZSGSRD 35167 17-03-18 00:09:08.618 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.618 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.618 -07 000000382 INF: FPGA: Recv ZSGSRD 35169 17-03-18 00:09:08.628 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.628 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:08.628 -07 000000547 INF: FPGA: Recv ZSGSRD 35173 17-03-18 00:09:08.638 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.638 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:08.638 -07 000000486 INF: FPGA: Recv ZPIEZORD 30813 17-03-18 00:09:08.648 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:08.648 -07 000000610 INF: Z Motor: Piezo is near 45.8 V (nominally 0 to 100 V), expect near 55.4 V, since DAC is at 5.542 V. Piezo Error = -9.6 V. 17-03-18 00:09:08.648 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -166.3 um, Read 10 ADC samples: Avg = 35172 (-165.4 uncal um), Error = 0.9 uncal um, Median = 35172 (-165.4 uncal um), StdDev = 4 (0.0 uncal um), Range = 13 (0.1 uncal um), Min = 35167 (-165.4 uncal um), Max = 35180 (-165.5 uncal um), Pos Noise = 8 (0.1 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:09:08.648 -07 000000610 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35172, posNoise = 8, negNoise = 5 17-03-18 00:09:08.648 -07 000000610 INF: Z Motor: After move to -0.166262, error = -0.000013, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:08.648 -07 000000610 INF: Z Motor: MoveAbsStepped took 272 ms) 17-03-18 00:09:08.648 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:08.648 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:08.648 -07 000000382 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:08.648 -07 000000012 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:08.648 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:08.648 -07 000000482 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:08.648 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:08.648 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:08.648 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:08.648 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:08.648 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:09:08.648 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:08.658 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:08.658 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:08.658 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:08.658 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:08.658 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 8 ms. 17-03-18 00:09:08.658 -07 000000012 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 12 ms. 17-03-18 00:09:08.658 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 8 ms. 17-03-18 00:09:08.658 -07 000000482 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 13 ms. 17-03-18 00:09:08.668 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36497 150\n [Camera] 17-03-18 00:09:08.868 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP turning red on 193 17-03-18 00:09:09.898 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:09.898 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:09.898 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:09.898 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:09.908 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) start. 17-03-18 00:09:09.908 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:09.908 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:09.908 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:09.908 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:09.908 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) start. 17-03-18 00:09:10.098 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36497 1422 17-03-18 00:09:10.248 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:10.288 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) end. Duration was 376 ms. 17-03-18 00:09:10.288 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 377 ms. 17-03-18 00:09:10.288 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:10.288 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:10.288 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) end. Duration was 384 ms. 17-03-18 00:09:10.288 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 384 ms. 17-03-18 00:09:10.288 -07 000000586 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:10.288 -07 000000586 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:10.288 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 388 ms. 17-03-18 00:09:10.298 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 387 ms. 17-03-18 00:09:10.748 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:10.748 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:10.748 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:10.748 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:10.748 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:10.748 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:10.748 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 2182910056, 0x0, 391868352) start. 17-03-18 00:09:10.748 -07 000000512 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:10.748 -07 000000512 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:10.748 -07 000000512 INF: Camera: sBCa_ReadImage(785074704, 2218332320, 0x0, 418435064) start. 17-03-18 00:09:10.938 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP 2264 17-03-18 00:09:10.938 -07 000000486 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:10.938 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36497 150' command took 2 ms to send, 2263 ms to get response, and 2272 ms overall 17-03-18 00:09:10.938 -07 000000610 INF: Skipping AutoFocus in Tile 16: adopting Best-Z from the cache: -0.166690 17-03-18 00:09:10.938 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 72.3626 mm, moving Z to -0.16669 mm 17-03-18 00:09:10.938 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:10.938 -07 000000627 INF: Y Motor: Entering MoveAbsStepped (target = 115780 steps, sync = True, waitForSettling = True) 17-03-18 00:09:10.938 -07 000000627 INF: Y Motor: Moving to 72.3626 mm, started at 71.5186 mm, delta = 0.8441 mm 17-03-18 00:09:10.938 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:10.938 -07 000000478 INF: Z Motor: Entering MoveAbsStepped (DAC = 36413, sync = True, waitForSettling = False) 17-03-18 00:09:10.938 -07 000000478 INF: Z Motor: Moving to -0.166688 mm, started at -0.166276 mm, distance = -0.41 um 17-03-18 00:09:10.948 -07 000000478 INF: FPGA: Xmit ZDACW 36413\n [Z Motor] 17-03-18 00:09:10.948 -07 000000547 INF: FPGA: Recv YRDPOS 146471 17-03-18 00:09:10.948 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 2 ms overall 17-03-18 00:09:11.058 -07 000000627 INF: Y Motor: Encoder = 146471 = motor step 114430.5 = 71.5190 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:11.058 -07 000000547 INF: FPGA: Recv ZDACW 17-03-18 00:09:11.058 -07 000000478 INF: FPGA: 'ZDACW 36413' command took 0 ms to send, 0 ms to get response, and 116 ms overall 17-03-18 00:09:11.058 -07 000000478 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:11.058 -07 000000627 INF: Y Motor: Last commanded position = 71.5326 mm (114452.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:11.058 -07 000000627 INF: Y Motor: Moving 1350 steps-29 17-03-18 00:09:11.068 -07 000000627 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:11.068 -07 000000486 INF: FPGA: Recv ZDACR 36413 17-03-18 00:09:11.068 -07 000000478 INF: FPGA: 'ZDACR' command took 0 ms to send, 1 ms to get response, and 6 ms overall 17-03-18 00:09:11.068 -07 000000478 INF: Z Motor: MoveAbsStepped took 123 ms (did NOT wait for settling yet)) 17-03-18 00:09:11.148 -07 000000512 INF: Camera: sBCa_ReadImage(785074704, 2218332320, 0x0, 418435064) end. Duration was 397 ms. 17-03-18 00:09:11.148 -07 000000512 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 397 ms. 17-03-18 00:09:11.148 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:11.148 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 2182910056, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:09:11.148 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:09:11.148 -07 000000476 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:11.148 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:11.148 -07 000000476 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:11.148 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 406 ms. 17-03-18 00:09:11.158 -07 000000512 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 409 ms. 17-03-18 00:09:11.438 -07 000000424 INF: FPGA: Recv YMVTILE 17-03-18 00:09:11.438 -07 000000627 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 379 ms overall 17-03-18 00:09:11.488 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:11.488 -07 000000478 INF: FPGA: Recv YRDPOS 148172 17-03-18 00:09:11.498 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:11.498 -07 000000627 INF: Y Motor: Encoder = 148172 = motor step 115759.4 = 72.3496 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:11.498 -07 000000627 INF: Y Motor: Position error before settling (table move) = -27 encoder counts (-0.013 mm) 17-03-18 00:09:11.498 -07 000000627 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 379 ms, distance = 0.844 mm, average velocity = 2.227 mm/sec 17-03-18 00:09:12.138 -07 000000502 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:12.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:12.448 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:12.448 -07 000000486 INF: FPGA: Recv YRDPOS 148172 17-03-18 00:09:12.458 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:12.458 -07 000000627 INF: Y Motor: Encoder = 148172 = motor step 115759.4 = 72.3496 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:12.478 -07 000000627 INF: Y Motor: After move to 72.362610, error = -0.013000, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:12.478 -07 000000627 INF: Y Motor: Position error after settling (table move) = -27 encoder counts (-0.013 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:12.478 -07 000000627 INF: Y Motor: Moved to 72.3496 after 1539 ms (table move) 17-03-18 00:09:12.478 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.166688 mm (DAC = 36413) 17-03-18 00:09:12.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.488 -07 000000547 INF: FPGA: Recv ZSGSRD 35239 17-03-18 00:09:12.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 2 ms to get response, and 2 ms overall 17-03-18 00:09:12.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.488 -07 000000586 INF: FPGA: Recv ZSGSRD 35237 17-03-18 00:09:12.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:12.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.498 -07 000000627 INF: FPGA: Recv ZSGSRD 35237 17-03-18 00:09:12.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.508 -07 000000569 INF: FPGA: Recv ZSGSRD 35239 17-03-18 00:09:12.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.518 -07 000000627 INF: FPGA: Recv ZSGSRD 35239 17-03-18 00:09:12.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.528 -07 000000382 INF: FPGA: Recv ZSGSRD 35235 17-03-18 00:09:12.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.538 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.538 -07 000000482 INF: FPGA: Recv ZSGSRD 35235 17-03-18 00:09:12.548 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.548 -07 000000569 INF: FPGA: Recv ZSGSRD 35236 17-03-18 00:09:12.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.558 -07 000000382 INF: FPGA: Recv ZSGSRD 35238 17-03-18 00:09:12.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.568 -07 000000627 INF: FPGA: Recv ZSGSRD 35233 17-03-18 00:09:12.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.578 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:12.578 -07 000000382 INF: FPGA: Recv ZPIEZORD 30904 17-03-18 00:09:12.588 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.588 -07 000000610 INF: Z Motor: Piezo is near 46.0 V (nominally 0 to 100 V), expect near 55.6 V, since DAC is at 5.556 V. Piezo Error = -9.5 V. 17-03-18 00:09:12.588 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -166.7 um, Read 10 ADC samples: Avg = 35237 (-165.8 uncal um), Error = 0.9 uncal um, Median = 35237 (-165.8 uncal um), StdDev = 2 (0.0 uncal um), Range = 6 (0.0 uncal um), Min = 35233 (-165.8 uncal um), Max = 35239 (-165.8 uncal um), Pos Noise = 2 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:09:12.588 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35237, posNoise = 2, negNoise = 4 17-03-18 00:09:12.588 -07 000000610 INF: Z Motor: After move to -0.166688, error = -0.000005, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:12.588 -07 000000610 INF: Z Motor: Settle wait time = 1541 ms, measure time = 101 ms (allowed 150 ms), total time = 1642 ms 17-03-18 00:09:12.588 -07 000000610 INF: Z Motor: Waiting extra 49 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:12.638 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1694 ms 17-03-18 00:09:12.638 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:12.638 -07 000000610 INF: ------------ Scan Snapping tile 16 at Y-position 72.349609375, Z-position -0.166693315250366. ---------- 17-03-18 00:09:12.638 -07 000000610 INF: Cycle 82 Tile 16 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:12.638 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36237, sync = True, waitForSettling = True) 17-03-18 00:09:12.638 -07 000000610 INF: Z Motor: Moving to -0.165882 mm, started at -0.166693 mm, distance = 0.81 um 17-03-18 00:09:12.638 -07 000000610 INF: FPGA: Xmit ZDACW 36237\n [Z Motor] 17-03-18 00:09:12.638 -07 000000569 INF: FPGA: Recv ZDACW 17-03-18 00:09:12.648 -07 000000610 INF: FPGA: 'ZDACW 36237' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:09:12.658 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:12.658 -07 000000482 INF: FPGA: Recv ZDACR 36237 17-03-18 00:09:12.668 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:12.668 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:12.818 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.818 -07 000000382 INF: FPGA: Recv ZSGSRD 35115 17-03-18 00:09:12.828 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:12.828 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.828 -07 000000382 INF: FPGA: Recv ZSGSRD 35119 17-03-18 00:09:12.838 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.838 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.838 -07 000000627 INF: FPGA: Recv ZSGSRD 35114 17-03-18 00:09:12.848 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.848 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.848 -07 000000627 INF: FPGA: Recv ZSGSRD 35112 17-03-18 00:09:12.858 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.858 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.858 -07 000000569 INF: FPGA: Recv ZSGSRD 35114 17-03-18 00:09:12.868 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.868 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.868 -07 000000569 INF: FPGA: Recv ZSGSRD 35110 17-03-18 00:09:12.878 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.878 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.878 -07 000000561 INF: FPGA: Recv ZSGSRD 35113 17-03-18 00:09:12.888 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.888 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.888 -07 000000561 INF: FPGA: Recv ZSGSRD 35114 17-03-18 00:09:12.898 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.898 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.898 -07 000000382 INF: FPGA: Recv ZSGSRD 35111 17-03-18 00:09:12.908 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.908 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:12.908 -07 000000627 INF: FPGA: Recv ZSGSRD 35113 17-03-18 00:09:12.918 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:12.918 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:12.918 -07 000000569 INF: FPGA: Recv ZPIEZORD 30726 17-03-18 00:09:12.928 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:12.928 -07 000000610 INF: Z Motor: Piezo is near 45.6 V (nominally 0 to 100 V), expect near 55.3 V, since DAC is at 5.529 V. Piezo Error = -9.7 V. 17-03-18 00:09:12.928 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -165.9 um, Read 10 ADC samples: Avg = 35114 (-165.0 uncal um), Error = 0.8 uncal um, Median = 35114 (-165.0 uncal um), StdDev = 2 (0.0 uncal um), Range = 9 (0.1 uncal um), Min = 35110 (-165.0 uncal um), Max = 35119 (-165.1 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:09:12.928 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35114, posNoise = 5, negNoise = 4 17-03-18 00:09:12.928 -07 000000610 INF: Z Motor: After move to -0.165882, error = -0.000021, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:12.928 -07 000000610 INF: Z Motor: MoveAbsStepped took 286 ms) 17-03-18 00:09:12.928 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:12.928 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:12.928 -07 000000561 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:12.928 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:12.928 -07 000000569 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:12.928 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:12.928 -07 000000482 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:12.928 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:12.928 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 2 ms. 17-03-18 00:09:12.928 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:12.928 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 2 ms. 17-03-18 00:09:12.928 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:12.938 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:12.938 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:12.938 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 4 ms. 17-03-18 00:09:12.938 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:12.948 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 16 ms. 17-03-18 00:09:12.948 -07 000000569 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 22 ms. 17-03-18 00:09:12.948 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:09:12.948 -07 000000482 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 23 ms. 17-03-18 00:09:12.948 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36413 150\n [Camera] 17-03-18 00:09:12.978 -07 000000569 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:09:13.168 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP turning red on 199 17-03-18 00:09:14.198 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:14.198 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:14.198 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:14.198 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:14.198 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:14.198 -07 000000438 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:14.198 -07 000000438 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:14.198 -07 000000438 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:14.208 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:14.208 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) start. 17-03-18 00:09:14.388 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36413 1422 17-03-18 00:09:14.538 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:14.598 -07 000000438 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 397 ms. 17-03-18 00:09:14.598 -07 000000438 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 398 ms. 17-03-18 00:09:14.598 -07 000000438 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:14.598 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) end. Duration was 395 ms. 17-03-18 00:09:14.598 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 395 ms. 17-03-18 00:09:14.598 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:14.608 -07 000000438 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:14.608 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:14.608 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 408 ms. 17-03-18 00:09:14.608 -07 000000438 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 409 ms. 17-03-18 00:09:15.038 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:15.038 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:15.038 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:15.038 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:15.038 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) start. 17-03-18 00:09:15.038 -07 000000569 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:15.038 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:15.048 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:15.048 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:15.048 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) start. 17-03-18 00:09:15.228 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:15.228 -07 000000476 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:15.228 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36413 150' command took 2 ms to send, 2268 ms to get response, and 2278 ms overall 17-03-18 00:09:15.238 -07 000000610 INF: Skipping AutoFocus in Tile 17: adopting Best-Z from the cache: -0.166479 17-03-18 00:09:15.238 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 73.1926 mm, moving Z to -0.16648 mm 17-03-18 00:09:15.238 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:15.238 -07 000000547 INF: Z Motor: Entering MoveAbsStepped (DAC = 36367, sync = True, waitForSettling = False) 17-03-18 00:09:15.238 -07 000000547 INF: Z Motor: Moving to -0.166477 mm, started at -0.165903 mm, distance = -0.57 um 17-03-18 00:09:15.238 -07 000000547 INF: FPGA: Xmit ZDACW 36367\n [Z Motor] 17-03-18 00:09:15.238 -07 000000438 INF: Y Motor: Entering MoveAbsStepped (target = 117108 steps, sync = True, waitForSettling = True) 17-03-18 00:09:15.238 -07 000000438 INF: Y Motor: Moving to 73.1926 mm, started at 72.3496 mm, delta = 0.8430 mm 17-03-18 00:09:15.238 -07 000000448 INF: FPGA: Recv ZDACW 17-03-18 00:09:15.248 -07 000000547 INF: FPGA: 'ZDACW 36367' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:15.248 -07 000000547 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:15.248 -07 000000569 INF: FPGA: Recv ZDACR 36367 17-03-18 00:09:15.258 -07 000000547 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:15.258 -07 000000547 INF: Z Motor: MoveAbsStepped took 16 ms (did NOT wait for settling yet)) 17-03-18 00:09:15.258 -07 000000438 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:15.258 -07 000000561 INF: FPGA: Recv YRDPOS 148172 17-03-18 00:09:15.268 -07 000000438 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 26 ms overall 17-03-18 00:09:15.268 -07 000000438 INF: Y Motor: Encoder = 148172 = motor step 115759.4 = 72.3496 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:15.268 -07 000000438 INF: Y Motor: Last commanded position = 72.3626 mm (115780.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:15.268 -07 000000438 INF: Y Motor: Moving 1349 steps-27 17-03-18 00:09:15.268 -07 000000438 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:15.448 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) end. Duration was 404 ms. 17-03-18 00:09:15.448 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 404 ms. 17-03-18 00:09:15.448 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:15.448 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) end. Duration was 403 ms. 17-03-18 00:09:15.448 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 403 ms. 17-03-18 00:09:15.448 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:15.448 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 6 ms. 17-03-18 00:09:15.448 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 411 ms. 17-03-18 00:09:15.448 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:15.458 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 415 ms. 17-03-18 00:09:15.638 -07 000000476 INF: FPGA: Recv YMVTILE 17-03-18 00:09:15.638 -07 000000438 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 378 ms overall 17-03-18 00:09:15.688 -07 000000438 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:15.688 -07 000000547 INF: FPGA: Recv YRDPOS 149864 17-03-18 00:09:15.698 -07 000000438 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:15.698 -07 000000438 INF: Y Motor: Encoder = 149864 = motor step 117081.3 = 73.1758 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:15.698 -07 000000438 INF: Y Motor: Position error before settling (table move) = -34 encoder counts (-0.017 mm) 17-03-18 00:09:15.698 -07 000000438 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 378 ms, distance = 0.843 mm, average velocity = 2.230 mm/sec 17-03-18 00:09:16.648 -07 000000438 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:16.648 -07 000000382 INF: FPGA: Recv YRDPOS 149864 17-03-18 00:09:16.658 -07 000000438 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:16.658 -07 000000438 INF: Y Motor: Encoder = 149864 = motor step 117081.3 = 73.1758 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:16.678 -07 000000438 INF: Y Motor: After move to 73.192604, error = -0.016823, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:16.678 -07 000000438 INF: Y Motor: Position error after settling (table move) = -34 encoder counts (-0.017 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:16.678 -07 000000438 INF: Y Motor: Moved to 73.1758 after 1443 ms (table move) 17-03-18 00:09:16.678 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.166477 mm (DAC = 36367) 17-03-18 00:09:16.688 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.688 -07 000000627 INF: FPGA: Recv ZSGSRD 35204 17-03-18 00:09:16.698 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.698 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.698 -07 000000382 INF: FPGA: Recv ZSGSRD 35203 17-03-18 00:09:16.708 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.708 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.708 -07 000000627 INF: FPGA: Recv ZSGSRD 35212 17-03-18 00:09:16.718 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.718 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.718 -07 000000382 INF: FPGA: Recv ZSGSRD 35202 17-03-18 00:09:16.728 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.728 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.728 -07 000000448 INF: FPGA: Recv ZSGSRD 35202 17-03-18 00:09:16.738 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.738 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.738 -07 000000627 INF: FPGA: Recv ZSGSRD 35206 17-03-18 00:09:16.748 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.748 -07 000000482 INF: FPGA: Recv ZSGSRD 35203 17-03-18 00:09:16.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.758 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.758 -07 000000547 INF: FPGA: Recv ZSGSRD 35203 17-03-18 00:09:16.768 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.768 -07 000000482 INF: FPGA: Recv ZSGSRD 35203 17-03-18 00:09:16.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:16.778 -07 000000569 INF: FPGA: Recv ZSGSRD 35203 17-03-18 00:09:16.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.788 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:16.788 -07 000000482 INF: FPGA: Recv ZPIEZORD 30894 17-03-18 00:09:16.798 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.798 -07 000000610 INF: Z Motor: Piezo is near 46.0 V (nominally 0 to 100 V), expect near 55.5 V, since DAC is at 5.549 V. Piezo Error = -9.5 V. 17-03-18 00:09:16.798 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -166.5 um, Read 10 ADC samples: Avg = 35204 (-165.6 uncal um), Error = 0.9 uncal um, Median = 35203 (-165.6 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 35202 (-165.6 uncal um), Max = 35212 (-165.7 uncal um), Pos Noise = 8 (0.1 uncal um), Neg Noise = 2 (0.0 uncal um) 17-03-18 00:09:16.798 -07 000000610 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35204, posNoise = 8, negNoise = 2 17-03-18 00:09:16.798 -07 000000610 INF: Z Motor: After move to -0.166477, error = -0.000004, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:16.798 -07 000000610 INF: Z Motor: Settle wait time = 1446 ms, measure time = 110 ms (allowed 150 ms), total time = 1556 ms 17-03-18 00:09:16.798 -07 000000610 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:16.838 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1598 ms 17-03-18 00:09:16.838 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:16.838 -07 000000610 INF: ------------ Scan Snapping tile 17 at Y-position 73.17578125, Z-position -0.166481316249688. ---------- 17-03-18 00:09:16.838 -07 000000610 INF: Cycle 82 Tile 17 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:16.838 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36191, sync = True, waitForSettling = True) 17-03-18 00:09:16.838 -07 000000610 INF: Z Motor: Moving to -0.165672 mm, started at -0.166481 mm, distance = 0.81 um 17-03-18 00:09:16.838 -07 000000610 INF: FPGA: Xmit ZDACW 36191\n [Z Motor] 17-03-18 00:09:16.838 -07 000000448 INF: FPGA: Recv ZDACW 17-03-18 00:09:16.848 -07 000000610 INF: FPGA: 'ZDACW 36191' command took 0 ms to send, 0 ms to get response, and 2 ms overall 17-03-18 00:09:16.848 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:16.848 -07 000000569 INF: FPGA: Recv ZDACR 36191 17-03-18 00:09:16.858 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:16.858 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:17.008 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.008 -07 000000569 INF: FPGA: Recv ZSGSRD 35073 17-03-18 00:09:17.018 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:17.018 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.018 -07 000000569 INF: FPGA: Recv ZSGSRD 35080 17-03-18 00:09:17.028 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.028 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.028 -07 000000627 INF: FPGA: Recv ZSGSRD 35080 17-03-18 00:09:17.038 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.038 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.038 -07 000000438 INF: FPGA: Recv ZSGSRD 35083 17-03-18 00:09:17.048 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.048 -07 000000561 INF: FPGA: Recv ZSGSRD 35078 17-03-18 00:09:17.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.058 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.058 -07 000000482 INF: FPGA: Recv ZSGSRD 35071 17-03-18 00:09:17.068 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.068 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.068 -07 000000482 INF: FPGA: Recv ZSGSRD 35081 17-03-18 00:09:17.078 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.078 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.078 -07 000000476 INF: FPGA: Recv ZSGSRD 35080 17-03-18 00:09:17.088 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.088 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.088 -07 000000547 INF: FPGA: Recv ZSGSRD 35080 17-03-18 00:09:17.098 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.098 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:17.098 -07 000000627 INF: FPGA: Recv ZSGSRD 35080 17-03-18 00:09:17.108 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.108 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:17.108 -07 000000627 INF: FPGA: Recv ZPIEZORD 30703 17-03-18 00:09:17.118 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:17.118 -07 000000610 INF: Z Motor: Piezo is near 45.6 V (nominally 0 to 100 V), expect near 55.2 V, since DAC is at 5.522 V. Piezo Error = -9.6 V. 17-03-18 00:09:17.118 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -165.7 um, Read 10 ADC samples: Avg = 35079 (-164.8 uncal um), Error = 0.9 uncal um, Median = 35080 (-164.8 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 35071 (-164.8 uncal um), Max = 35083 (-164.8 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 8 (0.1 uncal um) 17-03-18 00:09:17.118 -07 000000610 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35079, posNoise = 4, negNoise = 8 17-03-18 00:09:17.118 -07 000000610 INF: Z Motor: After move to -0.165672, error = -0.000007, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:17.118 -07 000000610 INF: Z Motor: MoveAbsStepped took 274 ms) 17-03-18 00:09:17.118 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:17.118 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:17.118 -07 000000569 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:17.118 -07 000000476 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:17.118 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:17.118 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:17.118 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:17.118 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:17.118 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:17.118 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:17.128 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:09:17.128 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:17.128 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:09:17.128 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:17.128 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:17.128 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:17.138 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 9 ms. 17-03-18 00:09:17.138 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 14 ms. 17-03-18 00:09:17.138 -07 000000569 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:17.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:17.138 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 8 ms. 17-03-18 00:09:17.138 -07 000000476 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 14 ms. 17-03-18 00:09:17.138 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36367 150\n [Camera] 17-03-18 00:09:17.338 -07 000000569 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:09:18.338 -07 000000569 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:09:18.378 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:18.378 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:18.378 -07 000000569 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:18.378 -07 000000569 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:18.378 -07 000000569 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) start. 17-03-18 00:09:18.378 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:18.378 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:18.378 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:18.378 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:18.378 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 1113297000, 0x0, 418435064) start. 17-03-18 00:09:18.568 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36367 1422 17-03-18 00:09:18.718 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:18.778 -07 000000569 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) end. Duration was 400 ms. 17-03-18 00:09:18.778 -07 000000569 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 401 ms. 17-03-18 00:09:18.778 -07 000000569 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:18.778 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 1113297000, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:09:18.778 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:09:18.778 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:18.778 -07 000000569 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:18.788 -07 000000569 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 405 ms. 17-03-18 00:09:18.788 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:18.788 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:09:19.218 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:19.218 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:19.218 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:19.218 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:19.218 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1148719264, 0x0, 391868352) start. 17-03-18 00:09:19.218 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:19.218 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:19.218 -07 000000476 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:19.218 -07 000000476 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:19.228 -07 000000476 INF: Camera: sBCa_ReadImage(785074704, 2281705520, 0x0, 418435064) start. 17-03-18 00:09:19.408 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:19.408 -07 000000569 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:19.408 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36367 150' command took 2 ms to send, 2268 ms to get response, and 2272 ms overall 17-03-18 00:09:19.408 -07 000000610 INF: Skipping AutoFocus in Tile 18: adopting Best-Z from the cache: -0.166011 17-03-18 00:09:19.418 -07 000000610 INF: Y Motor: MoveYZ optimized starting. Moving Y to 74.0226 mm, moving Z to -0.16601 mm 17-03-18 00:09:19.418 -07 000000610 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:19.418 -07 000000626 INF: Y Motor: Entering MoveAbsStepped (target = 118436 steps, sync = True, waitForSettling = True) 17-03-18 00:09:19.418 -07 000000626 INF: Y Motor: Moving to 74.0226 mm, started at 73.1758 mm, delta = 0.8468 mm 17-03-18 00:09:19.418 -07 000000626 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:19.428 -07 000000569 INF: Z Motor: Entering MoveAbsStepped (DAC = 36265, sync = True, waitForSettling = False) 17-03-18 00:09:19.428 -07 000000569 INF: Z Motor: Moving to -0.166011 mm, started at -0.165678 mm, distance = -0.33 um 17-03-18 00:09:19.428 -07 000000502 INF: FPGA: Recv YRDPOS 149864 17-03-18 00:09:19.438 -07 000000626 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:19.438 -07 000000626 INF: Y Motor: Encoder = 149864 = motor step 117081.3 = 73.1758 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:19.438 -07 000000626 INF: Y Motor: Last commanded position = 73.1926 mm (117108.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:19.438 -07 000000626 INF: Y Motor: Moving 1355 steps-34 17-03-18 00:09:19.438 -07 000000569 INF: FPGA: Xmit ZDACW 36265\n [Z Motor] 17-03-18 00:09:19.438 -07 000000627 INF: FPGA: Recv ZDACW 17-03-18 00:09:19.448 -07 000000569 INF: FPGA: 'ZDACW 36265' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:19.448 -07 000000626 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:19.458 -07 000000569 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:19.458 -07 000000608 INF: FPGA: Recv ZDACR 36265 17-03-18 00:09:19.468 -07 000000569 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:19.468 -07 000000569 INF: Z Motor: MoveAbsStepped took 40 ms (did NOT wait for settling yet)) 17-03-18 00:09:19.608 -07 000000476 INF: Camera: sBCa_ReadImage(785074704, 2281705520, 0x0, 418435064) end. Duration was 384 ms. 17-03-18 00:09:19.608 -07 000000476 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 384 ms. 17-03-18 00:09:19.608 -07 000000476 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:19.608 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1148719264, 0x0, 391868352) end. Duration was 390 ms. 17-03-18 00:09:19.608 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 390 ms. 17-03-18 00:09:19.608 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:19.608 -07 000000476 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:19.618 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:19.618 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:09:19.618 -07 000000476 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 395 ms. 17-03-18 00:09:19.818 -07 000000627 INF: FPGA: Recv YMVTILE 17-03-18 00:09:19.818 -07 000000626 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 388 ms overall 17-03-18 00:09:19.858 -07 000000626 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:19.868 -07 000000502 INF: FPGA: Recv YRDPOS 151569 17-03-18 00:09:19.868 -07 000000626 INF: FPGA: 'YRDPOS' command took 0 ms to send, 4 ms to get response, and 10 ms overall 17-03-18 00:09:19.868 -07 000000626 INF: Y Motor: Encoder = 151569 = motor step 118413.3 = 74.0083 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:19.868 -07 000000626 INF: Y Motor: Position error before settling (table move) = -29 encoder counts (-0.014 mm) 17-03-18 00:09:19.868 -07 000000626 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 388 ms, distance = 0.847 mm, average velocity = 2.183 mm/sec 17-03-18 00:09:20.818 -07 000000626 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:20.818 -07 000000476 INF: FPGA: Recv YRDPOS 151569 17-03-18 00:09:20.828 -07 000000626 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:20.828 -07 000000626 INF: Y Motor: Encoder = 151569 = motor step 118413.3 = 74.0083 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:20.838 -07 000000626 INF: Y Motor: After move to 74.022606, error = -0.014305, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:20.838 -07 000000626 INF: Y Motor: Position error after settling (table move) = -29 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:20.848 -07 000000626 INF: Y Motor: Moved to 74.0083 after 1420 ms (table move) 17-03-18 00:09:20.848 -07 000000610 INF: Z Motor: EndOfMoveAbsStepped: target = -0.166011 mm (DAC = 36265) 17-03-18 00:09:20.848 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.848 -07 000000476 INF: FPGA: Recv ZSGSRD 35134 17-03-18 00:09:20.858 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:20.858 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.858 -07 000000608 INF: FPGA: Recv ZSGSRD 35131 17-03-18 00:09:20.868 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.868 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.868 -07 000000501 INF: FPGA: Recv ZSGSRD 35130 17-03-18 00:09:20.878 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.878 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.878 -07 000000512 INF: FPGA: Recv ZSGSRD 35131 17-03-18 00:09:20.888 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.888 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.888 -07 000000012 INF: FPGA: Recv ZSGSRD 35131 17-03-18 00:09:20.898 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.898 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.898 -07 000000512 INF: FPGA: Recv ZSGSRD 35133 17-03-18 00:09:20.908 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.908 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.908 -07 000000627 INF: FPGA: Recv ZSGSRD 35141 17-03-18 00:09:20.918 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.918 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.918 -07 000000502 INF: FPGA: Recv ZSGSRD 35133 17-03-18 00:09:20.928 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.928 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.928 -07 000000608 INF: FPGA: Recv ZSGSRD 35128 17-03-18 00:09:20.938 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.938 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:20.938 -07 000000486 INF: FPGA: Recv ZSGSRD 35131 17-03-18 00:09:20.948 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.948 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:20.948 -07 000000608 INF: FPGA: Recv ZPIEZORD 30777 17-03-18 00:09:20.958 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:20.958 -07 000000610 INF: Z Motor: Piezo is near 45.7 V (nominally 0 to 100 V), expect near 55.3 V, since DAC is at 5.534 V. Piezo Error = -9.6 V. 17-03-18 00:09:20.958 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -166.0 um, Read 10 ADC samples: Avg = 35132 (-165.2 uncal um), Error = 0.9 uncal um, Median = 35131 (-165.1 uncal um), StdDev = 3 (0.0 uncal um), Range = 13 (0.1 uncal um), Min = 35128 (-165.1 uncal um), Max = 35141 (-165.2 uncal um), Pos Noise = 9 (0.1 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:09:20.958 -07 000000610 INF: Z Motor: ADC noise = 9, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35132, posNoise = 9, negNoise = 4 17-03-18 00:09:20.958 -07 000000610 INF: Z Motor: After move to -0.166011, error = -0.000008, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:20.958 -07 000000610 INF: Z Motor: Settle wait time = 1422 ms, measure time = 108 ms (allowed 150 ms), total time = 1530 ms 17-03-18 00:09:20.958 -07 000000610 INF: Z Motor: Waiting extra 42 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:21.008 -07 000000610 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1589 ms 17-03-18 00:09:21.008 -07 000000610 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:21.008 -07 000000610 INF: ------------ Scan Snapping tile 18 at Y-position 74.00830078125, Z-position -0.166018772975481. ---------- 17-03-18 00:09:21.008 -07 000000610 INF: Cycle 82 Tile 18 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:21.008 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36088, sync = True, waitForSettling = True) 17-03-18 00:09:21.008 -07 000000610 INF: Z Motor: Moving to -0.165200 mm, started at -0.166019 mm, distance = 0.82 um 17-03-18 00:09:21.008 -07 000000610 INF: FPGA: Xmit ZDACW 36088\n [Z Motor] 17-03-18 00:09:21.008 -07 000000478 INF: FPGA: Recv ZDACW 17-03-18 00:09:21.018 -07 000000610 INF: FPGA: 'ZDACW 36088' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:09:21.018 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:21.018 -07 000000501 INF: FPGA: Recv ZDACR 36088 17-03-18 00:09:21.028 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:21.028 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:21.178 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.178 -07 000000478 INF: FPGA: Recv ZSGSRD 35001 17-03-18 00:09:21.188 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:21.188 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.188 -07 000000012 INF: FPGA: Recv ZSGSRD 35009 17-03-18 00:09:21.198 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:21.198 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.198 -07 000000501 INF: FPGA: Recv ZSGSRD 35004 17-03-18 00:09:21.208 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:21.208 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.208 -07 000000627 INF: FPGA: Recv ZSGSRD 35004 17-03-18 00:09:21.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:21.218 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.218 -07 000000512 INF: FPGA: Recv ZSGSRD 35002 17-03-18 00:09:21.228 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:21.228 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.228 -07 000000486 INF: FPGA: Recv ZSGSRD 35005 17-03-18 00:09:21.238 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:21.238 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.238 -07 000000012 INF: FPGA: Recv ZSGSRD 35013 17-03-18 00:09:21.248 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:21.248 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.248 -07 000000627 INF: FPGA: Recv ZSGSRD 35013 17-03-18 00:09:21.258 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:21.258 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:21.258 -07 000000501 INF: FPGA: Recv ZSGSRD 35003 17-03-18 00:09:21.268 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:21.268 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:22.218 -07 000000012 INF: FPGA: Recv ZSGSRD 35008 17-03-18 00:09:22.218 -07 000000012 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:22.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 942 ms to get response, and 952 ms overall 17-03-18 00:09:22.218 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:22.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:22.218 -07 000000502 INF: FPGA: Recv ZPIEZORD 30634 17-03-18 00:09:22.228 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:22.228 -07 000000610 INF: Z Motor: Piezo is near 45.4 V (nominally 0 to 100 V), expect near 55.1 V, since DAC is at 5.507 V. Piezo Error = -9.6 V. 17-03-18 00:09:22.228 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -165.2 um, Read 10 ADC samples: Avg = 35006 (-164.3 uncal um), Error = 0.9 uncal um, Median = 35004 (-164.3 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 35001 (-164.3 uncal um), Max = 35013 (-164.4 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:09:22.228 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35006, posNoise = 7, negNoise = 5 17-03-18 00:09:22.228 -07 000000610 INF: Z Motor: After move to -0.165200, error = -0.000009, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:22.228 -07 000000610 INF: Z Motor: MoveAbsStepped took 1216 ms) 17-03-18 00:09:22.228 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:22.228 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:22.228 -07 000000012 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:22.228 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:22.228 -07 000000501 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:22.228 -07 000000486 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:22.228 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:22.228 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:09:22.228 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:22.228 -07 000000626 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:22.228 -07 000000486 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:09:22.228 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:22.228 -07 000000486 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:22.228 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:22.228 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:22.228 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:22.238 -07 000000486 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 9 ms. 17-03-18 00:09:22.238 -07 000000486 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 13 ms. 17-03-18 00:09:22.248 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 11 ms. 17-03-18 00:09:22.248 -07 000000012 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 15 ms. 17-03-18 00:09:22.248 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36265 150\n [Camera] 17-03-18 00:09:22.448 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:09:23.488 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:23.488 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:23.488 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:23.488 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:23.488 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 2317127784, 0x0, 391868352) start. 17-03-18 00:09:23.488 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:23.488 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:23.488 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:23.488 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:23.488 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 2352550048, 0x0, 418435064) start. 17-03-18 00:09:23.678 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36265 1422 17-03-18 00:09:23.698 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.027603, 17-03-18 00:09:23.828 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:23.888 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 2317127784, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:09:23.888 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:09:23.888 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:23.888 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 2352550048, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:09:23.888 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:09:23.888 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:23.888 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:23.888 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:09:23.888 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:23.898 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:09:24.328 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:24.328 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:24.458 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:24.458 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:24.458 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:24.458 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:24.458 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:09:24.458 -07 000000502 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:24.458 -07 000000502 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:24.458 -07 000000502 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:09:24.518 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:24.518 -07 000000012 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:24.518 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36265 150' command took 2 ms to send, 2266 ms to get response, and 2272 ms overall 17-03-18 00:09:24.518 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 0 - Best Z for this model is: -0.172880 17-03-18 00:09:24.518 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 19 at Y-position 74.00830078125, Z-Position -0.165209322245619. ------ 17-03-18 00:09:24.528 -07 000000486 INF: Y Motor: MoveYZ optimized starting. Moving Y to 74.8526 mm, moving Z to -0.17120 mm 17-03-18 00:09:24.528 -07 000000486 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:24.528 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:24.528 -07 000000561 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:24.528 -07 000000012 INF: Y Motor: Entering MoveAbsStepped (target = 119764 steps, sync = True, waitForSettling = True) 17-03-18 00:09:24.528 -07 000000012 INF: Y Motor: Moving to 74.8526 mm, started at 74.0083 mm, delta = 0.8443 mm 17-03-18 00:09:24.528 -07 000000478 INF: Z Motor: Entering MoveAbsStepped (DAC = 37399, sync = True, waitForSettling = False) 17-03-18 00:09:24.528 -07 000000478 INF: Z Motor: Moving to -0.171202 mm, started at -0.165209 mm, distance = -5.99 um 17-03-18 00:09:24.538 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:24.538 -07 000000478 INF: FPGA: Xmit ZDACW 37399\n [Z Motor] 17-03-18 00:09:24.538 -07 000000561 INF: FPGA: Recv ZDACW 17-03-18 00:09:24.548 -07 000000012 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:24.548 -07 000000478 INF: FPGA: 'ZDACW 37399' command took 0 ms to send, 0 ms to get response, and 12 ms overall 17-03-18 00:09:24.548 -07 000000561 INF: FPGA: Recv YRDPOS 151569 17-03-18 00:09:24.558 -07 000000512 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:09:24.558 -07 000000012 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 24 ms overall 17-03-18 00:09:24.558 -07 000000012 INF: Y Motor: Encoder = 151569 = motor step 118413.3 = 74.0083 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:24.558 -07 000000012 INF: Y Motor: Last commanded position = 74.0226 mm (118436.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:24.558 -07 000000012 INF: Y Motor: Moving 1351 steps-29 17-03-18 00:09:24.568 -07 000000478 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:24.568 -07 000000482 INF: FPGA: Recv ZDACR 37399 17-03-18 00:09:24.578 -07 000000478 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:09:24.578 -07 000000012 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:24.578 -07 000000478 INF: Z Motor: MoveAbsStepped took 42 ms (did NOT wait for settling yet)) 17-03-18 00:09:24.758 -07 000000561 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:09:24.758 -07 000000512 INF: FPGA: 'FOCMRRFOC' command took 2 ms to send, 194 ms to get response, and 224 ms overall 17-03-18 00:09:24.838 -07 000000502 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 384 ms. 17-03-18 00:09:24.838 -07 000000502 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 384 ms. 17-03-18 00:09:24.838 -07 000000502 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:24.848 -07 000000502 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:24.848 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 392 ms. 17-03-18 00:09:24.848 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 392 ms. 17-03-18 00:09:24.848 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:24.848 -07 000000502 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 395 ms. 17-03-18 00:09:24.848 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:24.848 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 397 ms. 17-03-18 00:09:24.948 -07 000000561 INF: FPGA: Recv YMVTILE 17-03-18 00:09:24.948 -07 000000012 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 395 ms overall 17-03-18 00:09:24.978 -07 000000012 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:24.978 -07 000000448 INF: FPGA: Recv YRDPOS 153256 17-03-18 00:09:24.988 -07 000000012 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:09:24.988 -07 000000012 INF: Y Motor: Encoder = 153256 = motor step 119731.3 = 74.8320 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:24.988 -07 000000012 INF: Y Motor: Position error before settling (table move) = -42 encoder counts (-0.021 mm) 17-03-18 00:09:24.988 -07 000000012 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 396 ms, distance = 0.844 mm, average velocity = 2.132 mm/sec 17-03-18 00:09:25.938 -07 000000012 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:25.938 -07 000000626 INF: FPGA: Recv YRDPOS 153256 17-03-18 00:09:25.948 -07 000000012 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:25.948 -07 000000012 INF: Y Motor: Encoder = 153256 = motor step 119731.3 = 74.8320 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:25.958 -07 000000012 INF: Y Motor: After move to 74.852608, error = -0.020576, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:25.958 -07 000000012 INF: Y Motor: Position error after settling (table move) = -42 encoder counts (-0.021 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:25.958 -07 000000012 INF: Y Motor: Moved to 74.8320 after 1430 ms (table move) 17-03-18 00:09:25.968 -07 000000486 INF: Z Motor: EndOfMoveAbsStepped: target = -0.171202 mm (DAC = 37399) 17-03-18 00:09:25.968 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:25.968 -07 000000512 INF: FPGA: Recv ZSGSRD 35938 17-03-18 00:09:25.978 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:25.978 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:25.978 -07 000000012 INF: FPGA: Recv ZSGSRD 35934 17-03-18 00:09:25.988 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:25.988 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:25.988 -07 000000482 INF: FPGA: Recv ZSGSRD 35934 17-03-18 00:09:25.998 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:25.998 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:25.998 -07 000000502 INF: FPGA: Recv ZSGSRD 35936 17-03-18 00:09:26.008 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.008 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:26.008 -07 000000561 INF: FPGA: Recv ZSGSRD 35937 17-03-18 00:09:26.018 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.018 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:26.018 -07 000000626 INF: FPGA: Recv ZSGSRD 35943 17-03-18 00:09:26.028 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.028 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:26.028 -07 000000448 INF: FPGA: Recv ZSGSRD 35937 17-03-18 00:09:26.038 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.038 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:26.038 -07 000000626 INF: FPGA: Recv ZSGSRD 35946 17-03-18 00:09:26.048 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.048 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:26.048 -07 000000448 INF: FPGA: Recv ZSGSRD 35943 17-03-18 00:09:26.058 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.058 -07 000000486 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:26.058 -07 000000626 INF: FPGA: Recv ZSGSRD 35938 17-03-18 00:09:26.068 -07 000000486 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.068 -07 000000486 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:26.068 -07 000000012 INF: FPGA: Recv ZPIEZORD 31839 17-03-18 00:09:26.078 -07 000000486 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.078 -07 000000486 INF: Z Motor: Piezo is near 48.0 V (nominally 0 to 100 V), expect near 57.1 V, since DAC is at 5.707 V. Piezo Error = -9.1 V. 17-03-18 00:09:26.078 -07 000000486 INF: Z Motor: ReadAdcAverage: Commanded Z = -171.2 um, Read 10 ADC samples: Avg = 35939 (-170.3 uncal um), Error = 0.9 uncal um, Median = 35938 (-170.3 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 35934 (-170.3 uncal um), Max = 35946 (-170.4 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:09:26.078 -07 000000486 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 35939, posNoise = 7, negNoise = 5 17-03-18 00:09:26.078 -07 000000486 INF: Z Motor: After move to -0.171202, error = -0.000001, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:26.078 -07 000000486 INF: Z Motor: Settle wait time = 1434 ms, measure time = 108 ms (allowed 150 ms), total time = 1542 ms 17-03-18 00:09:26.078 -07 000000486 INF: Z Motor: Waiting extra 42 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:26.128 -07 000000486 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1596 ms 17-03-18 00:09:26.128 -07 000000486 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:26.128 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:26.128 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:26.128 -07 000000482 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:26.128 -07 000000478 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:26.128 -07 000000502 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:09:26.128 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:26.128 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:26.128 -07 000000502 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:09:26.138 -07 000000502 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:26.138 -07 000000502 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:09:26.148 -07 000000502 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 12 ms. 17-03-18 00:09:26.148 -07 000000502 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 17 ms. 17-03-18 00:09:26.148 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:09:26.198 -07 000000512 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:09:26.498 -07 000000478 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:09:26.498 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:09:26.498 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:09:26.498 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:09:26.538 -07 000000448 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:09:26.538 -07 000000482 INF: FPGA: Recv CAMSNAP 17-03-18 00:09:26.538 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 382 ms to get response, and 384 ms overall 17-03-18 00:09:26.628 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 126 ms. 17-03-18 00:09:26.628 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:26.628 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:09:26.628 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 128 ms. 17-03-18 00:09:26.638 -07 000000627 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:26.638 -07 000000482 INF: Autofocus start 17-03-18 00:09:26.638 -07 000000482 INF: MeasureProfile start 17-03-18 00:09:26.638 -07 000000478 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:26.648 -07 000000627 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:09:26.648 -07 000000627 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:09:26.648 -07 000000482 INF: MeasureProfile done 17-03-18 00:09:26.648 -07 000000482 INF: X=112 Z correction [um]=0.5011052 17-03-18 00:09:26.648 -07 000000482 INF: Autofocus finished 17-03-18 00:09:26.848 -07 000000448 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:09:26.848 -07 000000627 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:09:26.848 -07 000000610 INF: Suspending Skipping AutoFocus for the next 6 cycles. 17-03-18 00:09:26.848 -07 000000610 INF: ------------ Scan Snapping tile 19 at Y-position 74.83203125, Z-position -0.171203112173882. ---------- 17-03-18 00:09:26.848 -07 000000610 INF: Cycle 82 Tile 19 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:26.858 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 36021, sync = True, waitForSettling = True) 17-03-18 00:09:26.858 -07 000000610 INF: Z Motor: Moving to -0.164894 mm, started at -0.171203 mm, distance = 6.31 um 17-03-18 00:09:26.858 -07 000000610 INF: FPGA: Xmit ZDACW 36021\n [Z Motor] 17-03-18 00:09:26.858 -07 000000482 INF: FPGA: Recv ZDACW 17-03-18 00:09:26.868 -07 000000610 INF: FPGA: 'ZDACW 36021' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:26.868 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:26.868 -07 000000482 INF: FPGA: Recv ZDACR 36021 17-03-18 00:09:26.878 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:26.878 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:27.028 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.028 -07 000000482 INF: FPGA: Recv ZSGSRD 34962 17-03-18 00:09:27.038 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:27.038 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.038 -07 000000502 INF: FPGA: Recv ZSGSRD 34953 17-03-18 00:09:27.048 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.048 -07 000000482 INF: FPGA: Recv ZSGSRD 34960 17-03-18 00:09:27.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.058 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.058 -07 000000502 INF: FPGA: Recv ZSGSRD 34958 17-03-18 00:09:27.068 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.068 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.068 -07 000000482 INF: FPGA: Recv ZSGSRD 34955 17-03-18 00:09:27.078 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.078 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.078 -07 000000512 INF: FPGA: Recv ZSGSRD 34957 17-03-18 00:09:27.088 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.088 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.088 -07 000000012 INF: FPGA: Recv ZSGSRD 34958 17-03-18 00:09:27.098 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.098 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.098 -07 000000482 INF: FPGA: Recv ZSGSRD 34961 17-03-18 00:09:27.108 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.108 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.108 -07 000000482 INF: FPGA: Recv ZSGSRD 34962 17-03-18 00:09:27.118 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.118 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:27.118 -07 000000012 INF: FPGA: Recv ZSGSRD 34959 17-03-18 00:09:27.128 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.128 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:27.128 -07 000000012 INF: FPGA: Recv ZPIEZORD 30532 17-03-18 00:09:27.138 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:27.138 -07 000000610 INF: Z Motor: Piezo is near 45.2 V (nominally 0 to 100 V), expect near 55.0 V, since DAC is at 5.496 V. Piezo Error = -9.7 V. 17-03-18 00:09:27.138 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -164.9 um, Read 10 ADC samples: Avg = 34958 (-164.0 uncal um), Error = 0.9 uncal um, Median = 34958 (-164.0 uncal um), StdDev = 3 (0.0 uncal um), Range = 9 (0.1 uncal um), Min = 34953 (-164.0 uncal um), Max = 34962 (-164.1 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:09:27.138 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 34958, posNoise = 4, negNoise = 5 17-03-18 00:09:27.138 -07 000000610 INF: Z Motor: After move to -0.164894, error = -0.000007, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:27.138 -07 000000610 INF: Z Motor: MoveAbsStepped took 279 ms) 17-03-18 00:09:27.138 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:27.138 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:27.138 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:27.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:27.138 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:27.138 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:27.138 -07 000000626 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:27.138 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:27.138 -07 000000482 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:27.138 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:27.138 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:09:27.138 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:27.138 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:27.138 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:27.148 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 2 ms. 17-03-18 00:09:27.148 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:27.148 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:09:27.148 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:27.158 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 13 ms. 17-03-18 00:09:27.158 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 18 ms. 17-03-18 00:09:27.158 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 13 ms. 17-03-18 00:09:27.158 -07 000000482 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 18 ms. 17-03-18 00:09:27.158 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 36197 150\n [Camera] 17-03-18 00:09:27.368 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning red on 199 17-03-18 00:09:28.398 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:28.398 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:28.398 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:28.398 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:28.398 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) start. 17-03-18 00:09:28.408 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:28.408 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:28.408 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:28.408 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:28.408 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) start. 17-03-18 00:09:28.598 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 36197 1422 17-03-18 00:09:28.748 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:28.788 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) end. Duration was 383 ms. 17-03-18 00:09:28.788 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 384 ms. 17-03-18 00:09:28.788 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:28.798 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) end. Duration was 391 ms. 17-03-18 00:09:28.798 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 391 ms. 17-03-18 00:09:28.798 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:28.798 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:28.798 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:28.798 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 395 ms. 17-03-18 00:09:28.798 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 395 ms. 17-03-18 00:09:29.058 -07 000000627 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:09:29.248 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:29.248 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:29.248 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:29.358 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:29.358 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:29.358 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:29.358 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:29.358 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:29.358 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:29.358 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:09:29.438 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:29.438 -07 000000502 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:29.438 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 36197 150' command took 2 ms to send, 2262 ms to get response, and 2275 ms overall 17-03-18 00:09:29.758 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 400 ms. 17-03-18 00:09:29.758 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:09:29.758 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:29.758 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 404 ms. 17-03-18 00:09:29.758 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 405 ms. 17-03-18 00:09:29.758 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:29.768 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:29.768 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:09:29.768 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 522 ms. 17-03-18 00:09:29.768 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074288, 0) start. 17-03-18 00:09:29.778 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 416 ms. 17-03-18 00:09:29.808 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074288, 0) end. Duration was 43 ms. 17-03-18 00:09:29.808 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074704, 0) start. 17-03-18 00:09:29.858 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074704, 0) end. Duration was 42 ms. 17-03-18 00:09:29.858 -07 000000610 INF: Move compensator for Bottom-surface imaging. 17-03-18 00:09:29.858 -07 000000610 INF: FPGA: Xmit EMCUR 80\n [Compensator] 17-03-18 00:09:29.858 -07 000000627 INF: FPGA: Recv EMCUR 17-03-18 00:09:29.868 -07 000000610 INF: FPGA: 'EMCUR 80' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:29.868 -07 000000610 INF: FPGA: Xmit EMIN 20 40\n [Compensator] 17-03-18 00:09:30.368 -07 000000478 INF: FPGA: Recv EMIN 17-03-18 00:09:30.368 -07 000000610 INF: FPGA: 'EMIN 20 40' command took 0 ms to send, 496 ms to get response, and 505 ms overall 17-03-18 00:09:30.368 -07 000000610 INF: FPGA: Xmit EMCUR 60\n [Compensator] 17-03-18 00:09:30.368 -07 000000502 INF: FPGA: Recv EMCUR 17-03-18 00:09:30.378 -07 000000610 INF: FPGA: 'EMCUR 60' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:09:30.378 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:09:30.378 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 1 at Y-position 74.83203125, Z-Position -0.164900960062814. ------ 17-03-18 00:09:30.378 -07 000000482 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:30.378 -07 000000448 INF: Y Motor: MoveYZ optimized starting. Moving Y to 59.9126 mm, moving Z to -0.13930 mm 17-03-18 00:09:30.378 -07 000000448 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:30.388 -07 000000561 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:30.388 -07 000000482 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:09:30.388 -07 000000482 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:09:30.388 -07 000000502 INF: Y Motor: Entering MoveAbsStepped (target = 95860 steps, sync = True, waitForSettling = True) 17-03-18 00:09:30.388 -07 000000502 INF: Y Motor: Moving to 59.9126 mm, started at 74.8320 mm, delta = -14.9194 mm 17-03-18 00:09:30.388 -07 000000627 INF: Z Motor: Entering MoveAbsStepped (DAC = 30431, sync = True, waitForSettling = False) 17-03-18 00:09:30.388 -07 000000627 INF: Z Motor: Moving to -0.139304 mm, started at -0.164901 mm, distance = 25.60 um 17-03-18 00:09:30.398 -07 000000627 INF: FPGA: Xmit ZDACW 30431\n [Z Motor] 17-03-18 00:09:30.398 -07 000000561 INF: FPGA: Recv ZDACW 17-03-18 00:09:30.408 -07 000000502 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:30.408 -07 000000627 INF: FPGA: 'ZDACW 30431' command took 0 ms to send, 0 ms to get response, and 18 ms overall 17-03-18 00:09:30.408 -07 000000561 INF: FPGA: Recv YRDPOS 153257 17-03-18 00:09:30.418 -07 000000502 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 28 ms overall 17-03-18 00:09:30.418 -07 000000502 INF: Y Motor: Encoder = 153257 = motor step 119732.0 = 74.8325 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:30.418 -07 000000627 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:30.418 -07 000000561 INF: FPGA: Recv ZDACR 30431 17-03-18 00:09:30.418 -07 000000502 INF: Y Motor: Last commanded position = 74.8526 mm (119764.2 steps), delta from last commanded position = -14.9400 mm (-23904.0 steps). UsingTableMove = False 17-03-18 00:09:30.418 -07 000000502 INF: Y Motor: Moving to 122701 encoder counts 17-03-18 00:09:30.428 -07 000000627 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:30.428 -07 000000627 INF: Z Motor: MoveAbsStepped took 38 ms (did NOT wait for settling yet)) 17-03-18 00:09:30.428 -07 000000502 INF: FPGA: Xmit YMVTO 122701\n [Y Motor] 17-03-18 00:09:30.588 -07 000000627 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:09:30.588 -07 000000482 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:09:32.048 -07 000000482 INF: FPGA: Recv YMVTO 122703 23904 17-03-18 00:09:32.048 -07 000000502 INF: FPGA: 'YMVTO 122701' command took 0 ms to send, 1619 ms to get response, and 1635 ms overall 17-03-18 00:09:32.048 -07 000000502 INF: Y Motor: Position error before settling = 2 encoder counts (0.001 mm) 17-03-18 00:09:32.048 -07 000000502 INF: Y Motor: To move from 74.833 to 59.913 (-14.919 mm) should take 23872 steps, but required 23904 steps (14.940 mm). Slippage = 0.020 mm (0.00%). 17-03-18 00:09:32.048 -07 000000502 INF: Y Motor: FPGA move command 'YMVTO 122701' took 1635 ms, distance = 14.919 mm, average velocity = 9.125 mm/sec 17-03-18 00:09:32.138 -07 000000482 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:32.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:34.418 -07 000000482 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:09:34.898 -07 000000502 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:34.898 -07 000000627 INF: FPGA: Recv YRDPOS 122698 17-03-18 00:09:34.908 -07 000000502 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:34.908 -07 000000502 INF: Y Motor: Encoder = 122698 = motor step 95857.8 = 59.9111 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:34.938 -07 000000502 INF: Y Motor: After move to 59.912609, error = -0.001476, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:34.938 -07 000000502 INF: Y Motor: Position error after settling = -3 encoder counts (-0.001 mm), Delta during settling = -5 counts (-0.002 mm) 17-03-18 00:09:34.938 -07 000000502 INF: Y Motor: Moved to 59.9111 after 4554 ms 17-03-18 00:09:34.938 -07 000000448 INF: Z Motor: EndOfMoveAbsStepped: target = -0.139304 mm (DAC = 30431) 17-03-18 00:09:34.938 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:34.948 -07 000000561 INF: FPGA: Recv ZSGSRD 30980 17-03-18 00:09:34.948 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 2 ms to get response, and 2 ms overall 17-03-18 00:09:34.948 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:34.948 -07 000000502 INF: FPGA: Recv ZSGSRD 30976 17-03-18 00:09:34.958 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:34.958 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:34.958 -07 000000482 INF: FPGA: Recv ZSGSRD 30973 17-03-18 00:09:34.968 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:34.968 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:34.968 -07 000000627 INF: FPGA: Recv ZSGSRD 30974 17-03-18 00:09:34.978 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:34.978 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:34.978 -07 000000478 INF: FPGA: Recv ZSGSRD 30976 17-03-18 00:09:34.988 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:34.988 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:34.988 -07 000000482 INF: FPGA: Recv ZSGSRD 30976 17-03-18 00:09:34.998 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:34.998 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:34.998 -07 000000478 INF: FPGA: Recv ZSGSRD 30967 17-03-18 00:09:35.008 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:35.008 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:35.008 -07 000000547 INF: FPGA: Recv ZSGSRD 30973 17-03-18 00:09:35.018 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:35.018 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:35.018 -07 000000478 INF: FPGA: Recv ZSGSRD 30970 17-03-18 00:09:35.028 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:35.028 -07 000000448 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:35.028 -07 000000547 INF: FPGA: Recv ZSGSRD 30975 17-03-18 00:09:35.038 -07 000000448 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:35.038 -07 000000448 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:35.038 -07 000000478 INF: FPGA: Recv ZPIEZORD 26402 17-03-18 00:09:35.048 -07 000000448 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:35.048 -07 000000448 INF: Z Motor: Piezo is near 36.4 V (nominally 0 to 100 V), expect near 46.4 V, since DAC is at 4.643 V. Piezo Error = -10.0 V. 17-03-18 00:09:35.048 -07 000000448 INF: Z Motor: ReadAdcAverage: Commanded Z = -139.3 um, Read 10 ADC samples: Avg = 30974 (-138.5 uncal um), Error = 0.8 uncal um, Median = 30974 (-138.5 uncal um), StdDev = 4 (0.0 uncal um), Range = 13 (0.1 uncal um), Min = 30967 (-138.5 uncal um), Max = 30980 (-138.5 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:09:35.048 -07 000000448 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30974, posNoise = 6, negNoise = 7 17-03-18 00:09:35.048 -07 000000448 INF: Z Motor: After move to -0.139304, error = -0.000003, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:35.048 -07 000000448 INF: Z Motor: Settle wait time = 4557 ms, measure time = 102 ms (allowed 150 ms), total time = 4659 ms 17-03-18 00:09:35.048 -07 000000448 INF: Z Motor: Waiting extra 48 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:35.098 -07 000000448 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 4712 ms 17-03-18 00:09:35.098 -07 000000448 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:35.098 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:35.098 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:35.098 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:35.098 -07 000000382 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:35.098 -07 000000547 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:09:35.098 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:35.098 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:09:35.098 -07 000000547 INF: Camera: sBCa_SetPowerMode(785074288, 1) start. 17-03-18 00:09:35.128 -07 000000547 INF: Camera: sBCa_SetPowerMode(785074288, 1) end. Duration was 20 ms. 17-03-18 00:09:35.128 -07 000000547 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:09:35.128 -07 000000547 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:35.128 -07 000000547 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:09:35.128 -07 000000547 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 6 ms. 17-03-18 00:09:35.128 -07 000000547 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 30 ms. 17-03-18 00:09:35.138 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:09:35.178 -07 000000448 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:09:35.478 -07 000000547 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:09:35.478 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:09:35.478 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:09:35.478 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:09:35.518 -07 000000382 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:09:35.518 -07 000000627 INF: FPGA: Recv CAMSNAP 17-03-18 00:09:35.518 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 376 ms to get response, and 384 ms overall 17-03-18 00:09:35.598 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 115 ms. 17-03-18 00:09:35.598 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:35.598 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:09:35.598 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 118 ms. 17-03-18 00:09:35.618 -07 000000382 INF: Image SNR check: start 17-03-18 00:09:35.618 -07 000000547 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:35.618 -07 000000448 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:35.628 -07 000000547 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:35.628 -07 000000547 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:09:35.628 -07 000000382 INF: Image SNR check: pass [peak (Max-Min)=43.72] 17-03-18 00:09:35.628 -07 000000382 INF: Autofocus start 17-03-18 00:09:35.628 -07 000000382 INF: MeasureProfile start 17-03-18 00:09:35.648 -07 000000382 INF: MeasureProfile done 17-03-18 00:09:35.648 -07 000000382 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:09:35.648 -07 000000382 INF: Autofocus finished 17-03-18 00:09:35.828 -07 000000482 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:09:35.828 -07 000000547 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:09:35.828 -07 000000610 INF: ------------ Scan Snapping tile 1 at Y-position 59.9111328125, Z-position -0.139306898890039. ---------- 17-03-18 00:09:35.828 -07 000000610 INF: Cycle 82 Tile 1 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:35.838 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28938, sync = True, waitForSettling = True) 17-03-18 00:09:35.838 -07 000000610 INF: Z Motor: Moving to -0.132470 mm, started at -0.139307 mm, distance = 6.84 um 17-03-18 00:09:35.838 -07 000000610 INF: FPGA: Xmit ZDACW 28938\n [Z Motor] 17-03-18 00:09:35.838 -07 000000482 INF: FPGA: Recv ZDACW 17-03-18 00:09:35.848 -07 000000610 INF: FPGA: 'ZDACW 28938' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:35.848 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:35.848 -07 000000561 INF: FPGA: Recv ZDACR 28938 17-03-18 00:09:35.858 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:35.858 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:36.008 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.008 -07 000000478 INF: FPGA: Recv ZSGSRD 29917 17-03-18 00:09:36.018 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:36.018 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.018 -07 000000627 INF: FPGA: Recv ZSGSRD 29911 17-03-18 00:09:36.028 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.028 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.028 -07 000000382 INF: FPGA: Recv ZSGSRD 29915 17-03-18 00:09:36.038 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.038 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.038 -07 000000561 INF: FPGA: Recv ZSGSRD 29907 17-03-18 00:09:36.048 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.048 -07 000000448 INF: FPGA: Recv ZSGSRD 29907 17-03-18 00:09:36.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 1 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.058 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.058 -07 000000448 INF: FPGA: Recv ZSGSRD 29913 17-03-18 00:09:36.068 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.068 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.068 -07 000000448 INF: FPGA: Recv ZSGSRD 29905 17-03-18 00:09:36.078 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.078 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.078 -07 000000448 INF: FPGA: Recv ZSGSRD 29913 17-03-18 00:09:36.088 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.088 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.088 -07 000000561 INF: FPGA: Recv ZSGSRD 29905 17-03-18 00:09:36.098 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.098 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:36.098 -07 000000561 INF: FPGA: Recv ZSGSRD 29913 17-03-18 00:09:36.108 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.108 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:36.108 -07 000000561 INF: FPGA: Recv ZPIEZORD 25225 17-03-18 00:09:36.118 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:36.118 -07 000000610 INF: Z Motor: Piezo is near 33.9 V (nominally 0 to 100 V), expect near 44.2 V, since DAC is at 4.416 V. Piezo Error = -10.3 V. 17-03-18 00:09:36.118 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -132.5 um, Read 10 ADC samples: Avg = 29911 (-131.7 uncal um), Error = 0.8 uncal um, Median = 29912 (-131.7 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 29905 (-131.7 uncal um), Max = 29917 (-131.7 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:09:36.118 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29911, posNoise = 6, negNoise = 6 17-03-18 00:09:36.118 -07 000000610 INF: Z Motor: After move to -0.132470, error = -0.000008, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:36.118 -07 000000610 INF: Z Motor: MoveAbsStepped took 279 ms) 17-03-18 00:09:36.118 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:36.118 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:36.118 -07 000000547 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:36.118 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:36.118 -07 000000438 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:36.118 -07 000000438 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:36.118 -07 000000424 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:36.118 -07 000000424 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:36.118 -07 000000438 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:36.118 -07 000000438 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:36.118 -07 000000424 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:09:36.118 -07 000000424 INF: Camera: sBCa_SetPowerMode(785074704, 1) start. 17-03-18 00:09:36.118 -07 000000438 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:36.118 -07 000000438 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:36.138 -07 000000438 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:09:36.138 -07 000000438 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 19 ms. 17-03-18 00:09:36.138 -07 000000424 INF: Camera: sBCa_SetPowerMode(785074704, 1) end. Duration was 22 ms. 17-03-18 00:09:36.148 -07 000000424 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:36.148 -07 000000424 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:36.148 -07 000000424 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:36.168 -07 000000424 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:09:36.168 -07 000000424 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 45 ms. 17-03-18 00:09:36.168 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 29115 150\n [Camera] 17-03-18 00:09:36.368 -07 000000438 INF: FPGA: Recv @CAMDBLSNAP turning red on 199 17-03-18 00:09:37.138 -07 000000561 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:37.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:37.408 -07 000000438 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:37.408 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:37.408 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:37.408 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:37.408 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) start. 17-03-18 00:09:37.408 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:37.408 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:37.408 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:37.408 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:37.408 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) start. 17-03-18 00:09:37.598 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 29115 1422 17-03-18 00:09:37.748 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:37.808 -07 000000478 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:09:37.808 -07 000000478 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:09:37.808 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:37.808 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:09:37.808 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:09:37.808 -07 000000424 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:37.808 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:37.808 -07 000000478 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:09:37.808 -07 000000424 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:37.818 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:09:38.248 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:38.248 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:38.248 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:38.358 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:38.358 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:38.358 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:38.358 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:38.358 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:38.368 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:38.368 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:09:38.438 -07 000000438 INF: FPGA: Recv @CAMDBLSNAP 2264 17-03-18 00:09:38.438 -07 000000627 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:38.438 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 29115 150' command took 2 ms to send, 2265 ms to get response, and 2272 ms overall 17-03-18 00:09:38.438 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:09:38.438 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 2 at Y-position 59.9111328125, Z-Position -0.132477961383347. ------ 17-03-18 00:09:38.448 -07 000000547 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:38.448 -07 000000438 INF: Y Motor: MoveYZ optimized starting. Moving Y to 60.7426 mm, moving Z to -0.13870 mm 17-03-18 00:09:38.448 -07 000000438 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:38.448 -07 000000561 INF: Y Motor: Entering MoveAbsStepped (target = 97188 steps, sync = True, waitForSettling = True) 17-03-18 00:09:38.448 -07 000000627 INF: Z Motor: Entering MoveAbsStepped (DAC = 30300, sync = True, waitForSettling = False) 17-03-18 00:09:38.448 -07 000000627 INF: Z Motor: Moving to -0.138705 mm, started at -0.132478 mm, distance = -6.23 um 17-03-18 00:09:38.448 -07 000000561 INF: Y Motor: Moving to 60.7426 mm, started at 59.9111 mm, delta = 0.8315 mm 17-03-18 00:09:38.448 -07 000000424 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:38.458 -07 000000547 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:38.458 -07 000000627 INF: FPGA: Xmit ZDACW 30300\n [Z Motor] 17-03-18 00:09:38.458 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:09:38.468 -07 000000627 INF: FPGA: 'ZDACW 30300' command took 0 ms to send, 0 ms to get response, and 14 ms overall 17-03-18 00:09:38.468 -07 000000561 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:38.468 -07 000000502 INF: FPGA: Recv YRDPOS 122698 17-03-18 00:09:38.478 -07 000000561 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 24 ms overall 17-03-18 00:09:38.478 -07 000000561 INF: Y Motor: Encoder = 122698 = motor step 95857.8 = 59.9111 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:38.478 -07 000000561 INF: Y Motor: Last commanded position = 59.9126 mm (95860.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:38.478 -07 000000561 INF: Y Motor: Moving 1330 steps2 17-03-18 00:09:38.478 -07 000000547 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:09:38.488 -07 000000627 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:38.488 -07 000000476 INF: FPGA: Recv ZDACR 30300 17-03-18 00:09:38.498 -07 000000561 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:38.498 -07 000000627 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:09:38.498 -07 000000627 INF: Z Motor: MoveAbsStepped took 45 ms (did NOT wait for settling yet)) 17-03-18 00:09:38.758 -07 000000448 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 398 ms. 17-03-18 00:09:38.758 -07 000000448 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:09:38.758 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:38.768 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 401 ms. 17-03-18 00:09:38.768 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 401 ms. 17-03-18 00:09:38.768 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:38.768 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:38.768 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:09:38.768 -07 000000448 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 522 ms. 17-03-18 00:09:38.778 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 412 ms. 17-03-18 00:09:38.778 -07 000000478 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:09:38.778 -07 000000547 INF: FPGA: 'FOCMRRFOC' command took 4 ms to send, 298 ms to get response, and 327 ms overall 17-03-18 00:09:38.868 -07 000000478 INF: FPGA: Recv YMVTILE 17-03-18 00:09:38.868 -07 000000561 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 394 ms overall 17-03-18 00:09:38.908 -07 000000561 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:38.908 -07 000000478 INF: FPGA: Recv YRDPOS 124381 17-03-18 00:09:38.918 -07 000000561 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:38.918 -07 000000561 INF: Y Motor: Encoder = 124381 = motor step 97172.7 = 60.7329 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:38.918 -07 000000561 INF: Y Motor: Position error before settling (table move) = -20 encoder counts (-0.010 mm) 17-03-18 00:09:38.918 -07 000000561 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 395 ms, distance = 0.831 mm, average velocity = 2.105 mm/sec 17-03-18 00:09:39.778 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:09:39.868 -07 000000561 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:39.868 -07 000000547 INF: FPGA: Recv YRDPOS 124381 17-03-18 00:09:39.878 -07 000000561 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:39.878 -07 000000561 INF: Y Motor: Encoder = 124381 = motor step 97172.7 = 60.7329 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:39.898 -07 000000561 INF: Y Motor: After move to 60.742607, error = -0.009697, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:39.898 -07 000000561 INF: Y Motor: Position error after settling (table move) = -20 encoder counts (-0.010 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:39.898 -07 000000561 INF: Y Motor: Moved to 60.7329 after 1453 ms (table move) 17-03-18 00:09:39.908 -07 000000438 INF: Z Motor: EndOfMoveAbsStepped: target = -0.138705 mm (DAC = 30300) 17-03-18 00:09:39.908 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.908 -07 000000478 INF: FPGA: Recv ZSGSRD 30882 17-03-18 00:09:39.918 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:39.918 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.918 -07 000000482 INF: FPGA: Recv ZSGSRD 30879 17-03-18 00:09:39.928 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:39.928 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.928 -07 000000561 INF: FPGA: Recv ZSGSRD 30883 17-03-18 00:09:39.938 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:39.938 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.938 -07 000000482 INF: FPGA: Recv ZSGSRD 30884 17-03-18 00:09:39.948 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:39.948 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.948 -07 000000478 INF: FPGA: Recv ZSGSRD 30884 17-03-18 00:09:39.958 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:39.958 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.958 -07 000000547 INF: FPGA: Recv ZSGSRD 30888 17-03-18 00:09:39.968 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:39.968 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.968 -07 000000478 INF: FPGA: Recv ZSGSRD 30880 17-03-18 00:09:39.978 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:39.978 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.978 -07 000000547 INF: FPGA: Recv ZSGSRD 30879 17-03-18 00:09:39.988 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:39.988 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.988 -07 000000561 INF: FPGA: Recv ZSGSRD 30880 17-03-18 00:09:39.998 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:39.998 -07 000000438 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:39.998 -07 000000586 INF: FPGA: Recv ZSGSRD 30882 17-03-18 00:09:40.008 -07 000000438 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:40.008 -07 000000438 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:40.008 -07 000000547 INF: FPGA: Recv ZPIEZORD 26548 17-03-18 00:09:40.018 -07 000000438 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:40.018 -07 000000438 INF: Z Motor: Piezo is near 36.7 V (nominally 0 to 100 V), expect near 46.2 V, since DAC is at 4.623 V. Piezo Error = -9.5 V. 17-03-18 00:09:40.018 -07 000000438 INF: Z Motor: ReadAdcAverage: Commanded Z = -138.7 um, Read 10 ADC samples: Avg = 30882 (-137.9 uncal um), Error = 0.8 uncal um, Median = 30882 (-137.9 uncal um), StdDev = 3 (0.0 uncal um), Range = 9 (0.1 uncal um), Min = 30879 (-137.9 uncal um), Max = 30888 (-138.0 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 3 (0.0 uncal um) 17-03-18 00:09:40.018 -07 000000438 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30882, posNoise = 6, negNoise = 3 17-03-18 00:09:40.018 -07 000000438 INF: Z Motor: After move to -0.138705, error = -0.000011, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:40.018 -07 000000438 INF: Z Motor: Settle wait time = 1455 ms, measure time = 110 ms (allowed 150 ms), total time = 1565 ms 17-03-18 00:09:40.018 -07 000000438 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:40.058 -07 000000438 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1607 ms 17-03-18 00:09:40.058 -07 000000438 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:40.058 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:40.058 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:40.058 -07 000000547 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:40.058 -07 000000561 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:40.058 -07 000000438 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:09:40.058 -07 000000438 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:40.058 -07 000000438 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:40.058 -07 000000438 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:09:40.058 -07 000000438 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:40.068 -07 000000438 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:09:40.078 -07 000000438 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 10 ms. 17-03-18 00:09:40.078 -07 000000438 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 16 ms. 17-03-18 00:09:40.078 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:09:40.128 -07 000000586 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:09:40.418 -07 000000478 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:09:40.428 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:09:40.428 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:09:40.428 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:09:40.458 -07 000000478 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:09:40.468 -07 000000547 INF: FPGA: Recv CAMSNAP 17-03-18 00:09:40.468 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 380 ms to get response, and 384 ms overall 17-03-18 00:09:40.548 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 126 ms. 17-03-18 00:09:40.548 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:40.558 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:09:40.558 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 129 ms. 17-03-18 00:09:40.568 -07 000000478 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:40.568 -07 000000438 INF: Autofocus start 17-03-18 00:09:40.568 -07 000000438 INF: MeasureProfile start 17-03-18 00:09:40.568 -07 000000482 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:40.578 -07 000000478 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:40.578 -07 000000478 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:09:40.578 -07 000000438 INF: MeasureProfile done 17-03-18 00:09:40.578 -07 000000438 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:09:40.578 -07 000000438 INF: Autofocus finished 17-03-18 00:09:40.778 -07 000000586 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:09:40.778 -07 000000478 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 195 ms to get response, and 202 ms overall 17-03-18 00:09:40.778 -07 000000610 INF: ------------ Scan Snapping tile 2 at Y-position 60.73291015625, Z-position -0.138715871372997. ---------- 17-03-18 00:09:40.788 -07 000000610 INF: Cycle 82 Tile 2 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:40.788 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28807, sync = True, waitForSettling = True) 17-03-18 00:09:40.788 -07 000000610 INF: Z Motor: Moving to -0.131870 mm, started at -0.138716 mm, distance = 6.85 um 17-03-18 00:09:40.788 -07 000000610 INF: FPGA: Xmit ZDACW 28807\n [Z Motor] 17-03-18 00:09:40.788 -07 000000586 INF: FPGA: Recv ZDACW 17-03-18 00:09:40.798 -07 000000610 INF: FPGA: 'ZDACW 28807' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:40.798 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:40.798 -07 000000482 INF: FPGA: Recv ZDACR 28807 17-03-18 00:09:40.808 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:40.808 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:40.958 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:40.958 -07 000000478 INF: FPGA: Recv ZSGSRD 29823 17-03-18 00:09:40.968 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:40.968 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:40.968 -07 000000561 INF: FPGA: Recv ZSGSRD 29817 17-03-18 00:09:40.978 -07 000000610 INF: FPGA: 'ZSGSRD' command took 1 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:40.978 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:40.978 -07 000000478 INF: FPGA: Recv ZSGSRD 29812 17-03-18 00:09:40.988 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:40.988 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:40.988 -07 000000561 INF: FPGA: Recv ZSGSRD 29819 17-03-18 00:09:40.998 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:40.998 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:40.998 -07 000000561 INF: FPGA: Recv ZSGSRD 29819 17-03-18 00:09:41.008 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:41.008 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:41.008 -07 000000478 INF: FPGA: Recv ZSGSRD 29820 17-03-18 00:09:41.018 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:41.018 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:41.018 -07 000000561 INF: FPGA: Recv ZSGSRD 29818 17-03-18 00:09:41.028 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:41.028 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:41.028 -07 000000478 INF: FPGA: Recv ZSGSRD 29818 17-03-18 00:09:41.038 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:41.038 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:41.038 -07 000000478 INF: FPGA: Recv ZSGSRD 29819 17-03-18 00:09:41.048 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:41.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:41.048 -07 000000561 INF: FPGA: Recv ZSGSRD 29815 17-03-18 00:09:41.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:41.058 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:41.058 -07 000000478 INF: FPGA: Recv ZPIEZORD 25171 17-03-18 00:09:41.068 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:41.068 -07 000000610 INF: Z Motor: Piezo is near 33.8 V (nominally 0 to 100 V), expect near 44.0 V, since DAC is at 4.396 V. Piezo Error = -10.2 V. 17-03-18 00:09:41.068 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -131.9 um, Read 10 ADC samples: Avg = 29818 (-131.1 uncal um), Error = 0.8 uncal um, Median = 29818 (-131.1 uncal um), StdDev = 3 (0.0 uncal um), Range = 11 (0.1 uncal um), Min = 29812 (-131.1 uncal um), Max = 29823 (-131.1 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:09:41.068 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29818, posNoise = 5, negNoise = 6 17-03-18 00:09:41.068 -07 000000610 INF: Z Motor: After move to -0.131870, error = -0.000011, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:41.068 -07 000000610 INF: Z Motor: MoveAbsStepped took 278 ms) 17-03-18 00:09:41.068 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:41.068 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:41.068 -07 000000438 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:41.068 -07 000000586 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:41.068 -07 000000501 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:41.068 -07 000000482 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:41.068 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:41.068 -07 000000501 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:41.068 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:09:41.068 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:41.068 -07 000000501 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:41.068 -07 000000501 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:41.078 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:41.078 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:41.078 -07 000000501 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:09:41.078 -07 000000501 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:41.088 -07 000000501 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 14 ms. 17-03-18 00:09:41.088 -07 000000501 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 19 ms. 17-03-18 00:09:41.088 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:09:41.088 -07 000000482 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:09:41.088 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28984 150\n [Camera] 17-03-18 00:09:41.288 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:09:42.138 -07 000000586 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:42.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:42.328 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:42.328 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:42.328 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:42.328 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:42.328 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) start. 17-03-18 00:09:42.328 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:42.328 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:42.328 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:42.338 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:42.338 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) start. 17-03-18 00:09:42.518 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28984 1422 17-03-18 00:09:42.678 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1575 17-03-18 00:09:42.728 -07 000000586 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:09:42.728 -07 000000586 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:09:42.728 -07 000000586 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:42.738 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:09:42.738 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:09:42.738 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:42.738 -07 000000586 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:42.738 -07 000000586 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:09:42.738 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:42.748 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:09:43.178 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2075 17-03-18 00:09:43.178 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2075 17-03-18 00:09:43.178 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:43.288 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2075 17-03-18 00:09:43.288 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2075 17-03-18 00:09:43.288 -07 000000501 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:43.288 -07 000000501 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:43.288 -07 000000501 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:43.288 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:43.288 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:09:43.368 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:43.368 -07 000000438 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:43.368 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28984 150' command took 2 ms to send, 2261 ms to get response, and 2272 ms overall 17-03-18 00:09:43.368 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:09:43.368 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 3 at Y-position 60.73291015625, Z-Position -0.131880509654163. ------ 17-03-18 00:09:43.368 -07 000000608 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:43.368 -07 000000512 INF: Y Motor: MoveYZ optimized starting. Moving Y to 61.5726 mm, moving Z to -0.13850 mm 17-03-18 00:09:43.368 -07 000000512 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:43.368 -07 000000586 INF: Y Motor: Entering MoveAbsStepped (target = 98516 steps, sync = True, waitForSettling = True) 17-03-18 00:09:43.368 -07 000000586 INF: Y Motor: Moving to 61.5726 mm, started at 60.7329 mm, delta = 0.8397 mm 17-03-18 00:09:43.368 -07 000000438 INF: Z Motor: Entering MoveAbsStepped (DAC = 30255, sync = True, waitForSettling = False) 17-03-18 00:09:43.368 -07 000000438 INF: Z Motor: Moving to -0.138499 mm, started at -0.131881 mm, distance = -6.62 um 17-03-18 00:09:43.368 -07 000000478 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:43.378 -07 000000438 INF: FPGA: Xmit ZDACW 30255\n [Z Motor] 17-03-18 00:09:43.378 -07 000000608 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:09:43.378 -07 000000482 INF: FPGA: Recv ZDACW 17-03-18 00:09:43.388 -07 000000586 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:43.388 -07 000000438 INF: FPGA: 'ZDACW 30255' command took 0 ms to send, 0 ms to get response, and 11 ms overall 17-03-18 00:09:43.388 -07 000000478 INF: FPGA: Recv YRDPOS 124382 17-03-18 00:09:43.398 -07 000000586 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 21 ms overall 17-03-18 00:09:43.398 -07 000000586 INF: Y Motor: Encoder = 124382 = motor step 97173.4 = 60.7334 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:43.398 -07 000000608 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:09:43.398 -07 000000586 INF: Y Motor: Last commanded position = 60.7426 mm (97188.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:43.398 -07 000000586 INF: Y Motor: Moving 1343 steps-20 17-03-18 00:09:43.408 -07 000000438 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:43.408 -07 000000486 INF: FPGA: Recv ZDACR 30255 17-03-18 00:09:43.418 -07 000000438 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:09:43.418 -07 000000438 INF: Z Motor: MoveAbsStepped took 41 ms (did NOT wait for settling yet)) 17-03-18 00:09:43.418 -07 000000586 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:43.598 -07 000000478 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:09:43.598 -07 000000608 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 193 ms to get response, and 222 ms overall 17-03-18 00:09:43.688 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 398 ms. 17-03-18 00:09:43.688 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:09:43.688 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:43.698 -07 000000501 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 402 ms. 17-03-18 00:09:43.698 -07 000000501 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 402 ms. 17-03-18 00:09:43.698 -07 000000501 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:43.698 -07 000000501 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:43.698 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:09:43.698 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 523 ms. 17-03-18 00:09:43.708 -07 000000501 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 413 ms. 17-03-18 00:09:43.788 -07 000000486 INF: FPGA: Recv YMVTILE 17-03-18 00:09:43.788 -07 000000586 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 389 ms overall 17-03-18 00:09:43.838 -07 000000586 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:43.838 -07 000000478 INF: FPGA: Recv YRDPOS 126083 17-03-18 00:09:43.848 -07 000000586 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:43.848 -07 000000586 INF: Y Motor: Encoder = 126083 = motor step 98502.3 = 61.5640 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:43.848 -07 000000586 INF: Y Motor: Position error before settling (table move) = -18 encoder counts (-0.009 mm) 17-03-18 00:09:43.848 -07 000000586 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 389 ms, distance = 0.840 mm, average velocity = 2.159 mm/sec 17-03-18 00:09:44.798 -07 000000586 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:44.798 -07 000000561 INF: FPGA: Recv YRDPOS 126083 17-03-18 00:09:44.808 -07 000000586 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:44.808 -07 000000586 INF: Y Motor: Encoder = 126083 = motor step 98502.3 = 61.5640 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:44.828 -07 000000586 INF: Y Motor: After move to 61.572609, error = -0.008644, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:44.828 -07 000000586 INF: Y Motor: Position error after settling (table move) = -18 encoder counts (-0.009 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:44.828 -07 000000586 INF: Y Motor: Moved to 61.5640 after 1460 ms (table move) 17-03-18 00:09:44.838 -07 000000512 INF: Z Motor: EndOfMoveAbsStepped: target = -0.138499 mm (DAC = 30255) 17-03-18 00:09:44.838 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.838 -07 000000586 INF: FPGA: Recv ZSGSRD 30850 17-03-18 00:09:44.848 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:44.848 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.848 -07 000000482 INF: FPGA: Recv ZSGSRD 30846 17-03-18 00:09:44.858 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.858 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.858 -07 000000482 INF: FPGA: Recv ZSGSRD 30855 17-03-18 00:09:44.868 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.868 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.878 -07 000000561 INF: FPGA: Recv ZSGSRD 30851 17-03-18 00:09:44.878 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:44.878 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.878 -07 000000561 INF: FPGA: Recv ZSGSRD 30851 17-03-18 00:09:44.888 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.888 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.888 -07 000000561 INF: FPGA: Recv ZSGSRD 30842 17-03-18 00:09:44.898 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.898 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.898 -07 000000561 INF: FPGA: Recv ZSGSRD 30847 17-03-18 00:09:44.908 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.908 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.908 -07 000000586 INF: FPGA: Recv ZSGSRD 30851 17-03-18 00:09:44.918 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.918 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.918 -07 000000586 INF: FPGA: Recv ZSGSRD 30850 17-03-18 00:09:44.928 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.928 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:44.928 -07 000000561 INF: FPGA: Recv ZSGSRD 30853 17-03-18 00:09:44.938 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.938 -07 000000512 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:44.938 -07 000000561 INF: FPGA: Recv ZPIEZORD 26564 17-03-18 00:09:44.948 -07 000000512 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:44.948 -07 000000512 INF: Z Motor: Piezo is near 36.7 V (nominally 0 to 100 V), expect near 46.2 V, since DAC is at 4.617 V. Piezo Error = -9.4 V. 17-03-18 00:09:44.948 -07 000000512 INF: Z Motor: ReadAdcAverage: Commanded Z = -138.5 um, Read 10 ADC samples: Avg = 30850 (-137.7 uncal um), Error = 0.8 uncal um, Median = 30850 (-137.7 uncal um), StdDev = 4 (0.0 uncal um), Range = 13 (0.1 uncal um), Min = 30842 (-137.7 uncal um), Max = 30855 (-137.7 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 8 (0.1 uncal um) 17-03-18 00:09:44.948 -07 000000512 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30850, posNoise = 5, negNoise = 8 17-03-18 00:09:44.948 -07 000000512 INF: Z Motor: After move to -0.138499, error = -0.000012, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:44.948 -07 000000512 INF: Z Motor: Settle wait time = 1463 ms, measure time = 108 ms (allowed 150 ms), total time = 1571 ms 17-03-18 00:09:44.948 -07 000000512 INF: Z Motor: Waiting extra 42 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:44.998 -07 000000512 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1623 ms 17-03-18 00:09:44.998 -07 000000512 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:44.998 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:44.998 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:44.998 -07 000000512 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:44.998 -07 000000561 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:09:44.998 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:44.998 -07 000000586 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:44.998 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:44.998 -07 000000561 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:09:45.008 -07 000000561 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:45.008 -07 000000561 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:09:45.008 -07 000000561 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 7 ms. 17-03-18 00:09:45.008 -07 000000561 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 11 ms. 17-03-18 00:09:45.018 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:09:45.058 -07 000000586 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:09:45.138 -07 000000561 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:09:45.358 -07 000000482 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:09:45.358 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:09:45.358 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:09:45.368 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:09:45.398 -07 000000512 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:09:45.398 -07 000000561 INF: FPGA: Recv CAMSNAP 17-03-18 00:09:45.398 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 376 ms to get response, and 384 ms overall 17-03-18 00:09:45.478 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 114 ms. 17-03-18 00:09:45.478 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:45.478 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:09:45.478 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 117 ms. 17-03-18 00:09:45.498 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:45.498 -07 000000561 INF: Autofocus start 17-03-18 00:09:45.498 -07 000000561 INF: MeasureProfile start 17-03-18 00:09:45.498 -07 000000627 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:45.508 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:45.508 -07 000000512 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:09:45.508 -07 000000561 INF: MeasureProfile done 17-03-18 00:09:45.508 -07 000000561 INF: X=16 Z correction [um]=0.0530179 17-03-18 00:09:45.508 -07 000000561 INF: Autofocus finished 17-03-18 00:09:45.708 -07 000000382 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:09:45.708 -07 000000512 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:09:45.708 -07 000000610 INF: ------------ Scan Snapping tile 3 at Y-position 61.56396484375, Z-position -0.138510296584461. ---------- 17-03-18 00:09:45.708 -07 000000610 INF: Cycle 82 Tile 3 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:45.718 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28779, sync = True, waitForSettling = True) 17-03-18 00:09:45.718 -07 000000610 INF: Z Motor: Moving to -0.131742 mm, started at -0.138510 mm, distance = 6.77 um 17-03-18 00:09:45.718 -07 000000610 INF: FPGA: Xmit ZDACW 28779\n [Z Motor] 17-03-18 00:09:45.718 -07 000000561 INF: FPGA: Recv ZDACW 17-03-18 00:09:45.728 -07 000000610 INF: FPGA: 'ZDACW 28779' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.728 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:45.728 -07 000000448 INF: FPGA: Recv ZDACR 28779 17-03-18 00:09:45.738 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.738 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:45.888 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.888 -07 000000482 INF: FPGA: Recv ZSGSRD 29801 17-03-18 00:09:45.898 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:45.898 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.898 -07 000000502 INF: FPGA: Recv ZSGSRD 29795 17-03-18 00:09:45.908 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.908 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.908 -07 000000382 INF: FPGA: Recv ZSGSRD 29795 17-03-18 00:09:45.918 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.918 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.918 -07 000000586 INF: FPGA: Recv ZSGSRD 29797 17-03-18 00:09:45.928 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.928 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.928 -07 000000448 INF: FPGA: Recv ZSGSRD 29795 17-03-18 00:09:45.938 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.938 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.938 -07 000000382 INF: FPGA: Recv ZSGSRD 29800 17-03-18 00:09:45.948 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.948 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.948 -07 000000561 INF: FPGA: Recv ZSGSRD 29794 17-03-18 00:09:45.958 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.958 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.958 -07 000000586 INF: FPGA: Recv ZSGSRD 29803 17-03-18 00:09:45.968 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.968 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.978 -07 000000561 INF: FPGA: Recv ZSGSRD 29798 17-03-18 00:09:45.978 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:45.978 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:45.978 -07 000000586 INF: FPGA: Recv ZSGSRD 29797 17-03-18 00:09:45.988 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.988 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:45.988 -07 000000627 INF: FPGA: Recv ZPIEZORD 25140 17-03-18 00:09:45.998 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:45.998 -07 000000610 INF: Z Motor: Piezo is near 33.7 V (nominally 0 to 100 V), expect near 43.9 V, since DAC is at 4.391 V. Piezo Error = -10.2 V. 17-03-18 00:09:45.998 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -131.7 um, Read 10 ADC samples: Avg = 29798 (-131.0 uncal um), Error = 0.8 uncal um, Median = 29797 (-131.0 uncal um), StdDev = 3 (0.0 uncal um), Range = 9 (0.1 uncal um), Min = 29794 (-130.9 uncal um), Max = 29803 (-131.0 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:09:45.998 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29798, posNoise = 5, negNoise = 4 17-03-18 00:09:45.998 -07 000000610 INF: Z Motor: After move to -0.131742, error = -0.000010, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:45.998 -07 000000610 INF: Z Motor: MoveAbsStepped took 283 ms) 17-03-18 00:09:45.998 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:45.998 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:45.998 -07 000000512 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:45.998 -07 000000382 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:45.998 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:45.998 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:45.998 -07 000000502 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:45.998 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:45.998 -07 000000382 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:45.998 -07 000000382 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:45.998 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:09:45.998 -07 000000502 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:46.008 -07 000000382 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:46.008 -07 000000382 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:46.008 -07 000000502 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:46.008 -07 000000502 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:46.018 -07 000000382 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 14 ms. 17-03-18 00:09:46.018 -07 000000382 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 19 ms. 17-03-18 00:09:46.018 -07 000000502 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:09:46.018 -07 000000502 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:09:46.028 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28956 150\n [Camera] 17-03-18 00:09:46.228 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:09:47.138 -07 000000448 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:47.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:47.258 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:47.258 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:47.258 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:47.258 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:47.258 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) start. 17-03-18 00:09:47.268 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:47.268 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:47.268 -07 000000502 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:47.268 -07 000000502 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:47.268 -07 000000502 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) start. 17-03-18 00:09:47.458 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28956 1422 17-03-18 00:09:47.608 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:47.638 -07 000000502 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) end. Duration was 376 ms. 17-03-18 00:09:47.638 -07 000000502 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 376 ms. 17-03-18 00:09:47.638 -07 000000502 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:47.648 -07 000000502 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:47.648 -07 000000502 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 386 ms. 17-03-18 00:09:47.658 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) end. Duration was 390 ms. 17-03-18 00:09:47.658 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 390 ms. 17-03-18 00:09:47.658 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:47.658 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:47.658 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:09:48.108 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:48.108 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:48.108 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:48.218 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:48.218 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:48.218 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:48.218 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:48.218 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:48.218 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:48.218 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:09:48.298 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:48.298 -07 000000561 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:48.298 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28956 150' command took 4 ms to send, 2262 ms to get response, and 2272 ms overall 17-03-18 00:09:48.298 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:09:48.298 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 4 at Y-position 61.56396484375, Z-Position -0.131752025411328. ------ 17-03-18 00:09:48.298 -07 000000586 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:48.298 -07 000000424 INF: Y Motor: MoveYZ optimized starting. Moving Y to 62.4026 mm, moving Z to -0.13815 mm 17-03-18 00:09:48.298 -07 000000424 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:48.308 -07 000000569 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:48.308 -07 000000586 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 2 ms to get response, and 3 ms overall 17-03-18 00:09:48.308 -07 000000448 INF: Y Motor: Entering MoveAbsStepped (target = 99844 steps, sync = True, waitForSettling = True) 17-03-18 00:09:48.308 -07 000000448 INF: Y Motor: Moving to 62.4026 mm, started at 61.5640 mm, delta = 0.8386 mm 17-03-18 00:09:48.308 -07 000000586 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:09:48.308 -07 000000482 INF: Z Motor: Entering MoveAbsStepped (DAC = 30178, sync = True, waitForSettling = False) 17-03-18 00:09:48.308 -07 000000482 INF: Z Motor: Moving to -0.138146 mm, started at -0.131752 mm, distance = -6.39 um 17-03-18 00:09:48.318 -07 000000482 INF: FPGA: Xmit ZDACW 30178\n [Z Motor] 17-03-18 00:09:48.318 -07 000000569 INF: FPGA: Recv ZDACW 17-03-18 00:09:48.328 -07 000000482 INF: FPGA: 'ZDACW 30178' command took 0 ms to send, 0 ms to get response, and 16 ms overall 17-03-18 00:09:48.328 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:48.328 -07 000000561 INF: FPGA: Recv YRDPOS 126083 17-03-18 00:09:48.338 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 27 ms overall 17-03-18 00:09:48.338 -07 000000482 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:48.338 -07 000000448 INF: Y Motor: Encoder = 126083 = motor step 98502.3 = 61.5640 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:48.338 -07 000000448 INF: Y Motor: Last commanded position = 61.5726 mm (98516.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:48.338 -07 000000448 INF: Y Motor: Moving 1342 steps-18 17-03-18 00:09:48.338 -07 000000502 INF: FPGA: Recv ZDACR 30178 17-03-18 00:09:48.348 -07 000000482 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:48.348 -07 000000482 INF: Z Motor: MoveAbsStepped took 36 ms (did NOT wait for settling yet)) 17-03-18 00:09:48.348 -07 000000448 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:48.508 -07 000000569 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:09:48.508 -07 000000586 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 195 ms to get response, and 202 ms overall 17-03-18 00:09:48.618 -07 000000382 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 397 ms. 17-03-18 00:09:48.618 -07 000000382 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 397 ms. 17-03-18 00:09:48.618 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:48.618 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:09:48.618 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 401 ms. 17-03-18 00:09:48.618 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:48.618 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:48.628 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:09:48.628 -07 000000382 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 520 ms. 17-03-18 00:09:48.628 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:09:48.718 -07 000000569 INF: FPGA: Recv YMVTILE 17-03-18 00:09:48.718 -07 000000448 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 385 ms overall 17-03-18 00:09:48.758 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:48.768 -07 000000569 INF: FPGA: Recv YRDPOS 127786 17-03-18 00:09:48.778 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:48.778 -07 000000448 INF: Y Motor: Encoder = 127786 = motor step 99832.8 = 62.3955 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:48.778 -07 000000448 INF: Y Motor: Position error before settling (table move) = -15 encoder counts (-0.007 mm) 17-03-18 00:09:48.778 -07 000000448 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 386 ms, distance = 0.839 mm, average velocity = 2.173 mm/sec 17-03-18 00:09:49.728 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:49.728 -07 000000512 INF: FPGA: Recv YRDPOS 127786 17-03-18 00:09:49.738 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:49.738 -07 000000448 INF: Y Motor: Encoder = 127786 = motor step 99832.8 = 62.3955 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:49.758 -07 000000448 INF: Y Motor: After move to 62.402607, error = -0.007099, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:49.758 -07 000000448 INF: Y Motor: Position error after settling (table move) = -15 encoder counts (-0.007 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:09:49.758 -07 000000448 INF: Y Motor: Moved to 62.3955 after 1454 ms (table move) 17-03-18 00:09:49.768 -07 000000424 INF: Z Motor: EndOfMoveAbsStepped: target = -0.138146 mm (DAC = 30178) 17-03-18 00:09:49.768 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:49.768 -07 000000448 INF: FPGA: Recv ZSGSRD 30795 17-03-18 00:09:49.778 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:49.778 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:49.778 -07 000000561 INF: FPGA: Recv ZSGSRD 30803 17-03-18 00:09:49.788 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:49.788 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:49.788 -07 000000502 INF: FPGA: Recv ZSGSRD 30794 17-03-18 00:09:49.798 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:49.808 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:50.718 -07 000000476 INF: FPGA: Recv ZSGSRD 30799 17-03-18 00:09:50.718 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 901 ms to get response, and 920 ms overall 17-03-18 00:09:50.718 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:50.718 -07 000000382 INF: FPGA: Recv ZSGSRD 30799 17-03-18 00:09:50.728 -07 000000424 INF: FPGA: 'ZSGSRD' command took 1 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:50.728 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:50.728 -07 000000476 INF: FPGA: Recv ZSGSRD 30797 17-03-18 00:09:50.738 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:50.738 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:50.738 -07 000000502 INF: FPGA: Recv ZSGSRD 30791 17-03-18 00:09:50.748 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:50.748 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:50.748 -07 000000627 INF: FPGA: Recv ZSGSRD 30789 17-03-18 00:09:50.758 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:50.758 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:50.758 -07 000000586 INF: FPGA: Recv ZSGSRD 30795 17-03-18 00:09:50.768 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:50.768 -07 000000424 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:50.768 -07 000000627 INF: FPGA: Recv ZSGSRD 30798 17-03-18 00:09:50.778 -07 000000424 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:50.778 -07 000000424 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:50.778 -07 000000382 INF: FPGA: Recv ZPIEZORD 26532 17-03-18 00:09:50.788 -07 000000424 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:50.788 -07 000000424 INF: Z Motor: Piezo is near 36.7 V (nominally 0 to 100 V), expect near 46.0 V, since DAC is at 4.605 V. Piezo Error = -9.4 V. 17-03-18 00:09:50.788 -07 000000424 INF: Z Motor: ReadAdcAverage: Commanded Z = -138.1 um, Read 10 ADC samples: Avg = 30796 (-137.4 uncal um), Error = 0.8 uncal um, Median = 30796 (-137.4 uncal um), StdDev = 4 (0.0 uncal um), Range = 14 (0.1 uncal um), Min = 30789 (-137.3 uncal um), Max = 30803 (-137.4 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:09:50.788 -07 000000424 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30796, posNoise = 7, negNoise = 7 17-03-18 00:09:50.788 -07 000000424 INF: Z Motor: After move to -0.138146, error = -0.000017, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:50.788 -07 000000424 INF: Z Motor: Settle wait time = 1456 ms, measure time = 1021 ms (allowed 150 ms), total time = 2477 ms 17-03-18 00:09:50.788 -07 000000424 INF: Z Motor: Warning: Actual position measuring time = 1021 ms, longer than max expected time of 150 ms 17-03-18 00:09:50.788 -07 000000424 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 2481 ms 17-03-18 00:09:50.788 -07 000000424 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:50.788 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:50.788 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:50.788 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:50.788 -07 000000476 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:50.788 -07 000000502 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:09:50.788 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:50.788 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:50.788 -07 000000502 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:09:50.798 -07 000000502 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:50.798 -07 000000502 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:09:50.808 -07 000000502 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 10 ms. 17-03-18 00:09:50.808 -07 000000502 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 14 ms. 17-03-18 00:09:50.808 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:09:50.858 -07 000000476 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:09:51.148 -07 000000561 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:09:51.158 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:09:51.158 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:09:51.158 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:09:51.188 -07 000000502 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:09:51.198 -07 000000382 INF: FPGA: Recv CAMSNAP 17-03-18 00:09:51.198 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 380 ms to get response, and 385 ms overall 17-03-18 00:09:51.278 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 126 ms. 17-03-18 00:09:51.278 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:51.288 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:09:51.288 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 129 ms. 17-03-18 00:09:51.298 -07 000000448 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:51.298 -07 000000476 INF: Autofocus start 17-03-18 00:09:51.298 -07 000000476 INF: MeasureProfile start 17-03-18 00:09:51.298 -07 000000424 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:51.308 -07 000000448 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:09:51.308 -07 000000448 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:09:51.308 -07 000000476 INF: MeasureProfile done 17-03-18 00:09:51.308 -07 000000476 INF: X=16 Z correction [um]=0.0530179 17-03-18 00:09:51.308 -07 000000476 INF: Autofocus finished 17-03-18 00:09:51.338 -07 000000561 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:09:51.508 -07 000000382 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:09:51.508 -07 000000448 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:09:51.508 -07 000000610 INF: ------------ Scan Snapping tile 4 at Y-position 62.3955078125, Z-position -0.138163389128805. ---------- 17-03-18 00:09:51.508 -07 000000610 INF: Cycle 82 Tile 4 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:51.518 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28702, sync = True, waitForSettling = True) 17-03-18 00:09:51.518 -07 000000610 INF: Z Motor: Moving to -0.131389 mm, started at -0.138163 mm, distance = 6.77 um 17-03-18 00:09:51.518 -07 000000610 INF: FPGA: Xmit ZDACW 28702\n [Z Motor] 17-03-18 00:09:51.518 -07 000000502 INF: FPGA: Recv ZDACW 17-03-18 00:09:51.528 -07 000000610 INF: FPGA: 'ZDACW 28702' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:09:51.528 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:51.528 -07 000000586 INF: FPGA: Recv ZDACR 28702 17-03-18 00:09:51.538 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.538 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:51.688 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.688 -07 000000569 INF: FPGA: Recv ZSGSRD 29742 17-03-18 00:09:51.698 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:51.698 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.698 -07 000000569 INF: FPGA: Recv ZSGSRD 29748 17-03-18 00:09:51.708 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.708 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.708 -07 000000448 INF: FPGA: Recv ZSGSRD 29738 17-03-18 00:09:51.718 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.718 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.718 -07 000000476 INF: FPGA: Recv ZSGSRD 29739 17-03-18 00:09:51.728 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.728 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.728 -07 000000627 INF: FPGA: Recv ZSGSRD 29740 17-03-18 00:09:51.738 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.738 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.738 -07 000000382 INF: FPGA: Recv ZSGSRD 29741 17-03-18 00:09:51.748 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.748 -07 000000627 INF: FPGA: Recv ZSGSRD 29748 17-03-18 00:09:51.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.758 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.758 -07 000000586 INF: FPGA: Recv ZSGSRD 29738 17-03-18 00:09:51.768 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.768 -07 000000476 INF: FPGA: Recv ZSGSRD 29742 17-03-18 00:09:51.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:51.778 -07 000000502 INF: FPGA: Recv ZSGSRD 29746 17-03-18 00:09:51.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.788 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:51.788 -07 000000382 INF: FPGA: Recv ZPIEZORD 25101 17-03-18 00:09:51.798 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:51.798 -07 000000610 INF: Z Motor: Piezo is near 33.6 V (nominally 0 to 100 V), expect near 43.8 V, since DAC is at 4.380 V. Piezo Error = -10.2 V. 17-03-18 00:09:51.798 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -131.4 um, Read 10 ADC samples: Avg = 29742 (-130.6 uncal um), Error = 0.8 uncal um, Median = 29742 (-130.6 uncal um), StdDev = 4 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 29738 (-130.6 uncal um), Max = 29748 (-130.6 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:09:51.798 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29742, posNoise = 6, negNoise = 4 17-03-18 00:09:51.798 -07 000000610 INF: Z Motor: After move to -0.131389, error = -0.000003, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:51.798 -07 000000610 INF: Z Motor: MoveAbsStepped took 280 ms) 17-03-18 00:09:51.798 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:51.798 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:51.798 -07 000000586 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:51.798 -07 000000502 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:51.798 -07 000000448 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:51.798 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:51.798 -07 000000424 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:51.798 -07 000000424 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:51.798 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:09:51.798 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:51.798 -07 000000424 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:51.798 -07 000000424 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:51.808 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:09:51.808 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:51.808 -07 000000424 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:09:51.808 -07 000000424 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:51.818 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 16 ms. 17-03-18 00:09:51.818 -07 000000448 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 22 ms. 17-03-18 00:09:51.818 -07 000000424 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:09:51.818 -07 000000424 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 22 ms. 17-03-18 00:09:51.828 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28879 150\n [Camera] 17-03-18 00:09:52.028 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:09:52.138 -07 000000448 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:52.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:53.058 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:53.058 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:53.058 -07 000000501 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:53.068 -07 000000501 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:53.068 -07 000000501 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) start. 17-03-18 00:09:53.068 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:53.068 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:53.068 -07 000000586 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:53.068 -07 000000586 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:53.068 -07 000000586 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) start. 17-03-18 00:09:53.368 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28879 1422 17-03-18 00:09:53.408 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:53.458 -07 000000501 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) end. Duration was 393 ms. 17-03-18 00:09:53.458 -07 000000501 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:09:53.458 -07 000000501 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:53.458 -07 000000586 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) end. Duration was 392 ms. 17-03-18 00:09:53.458 -07 000000586 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 392 ms. 17-03-18 00:09:53.458 -07 000000586 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:53.458 -07 000000501 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:53.458 -07 000000501 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 398 ms. 17-03-18 00:09:53.458 -07 000000586 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:53.468 -07 000000586 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 404 ms. 17-03-18 00:09:53.908 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:53.908 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:53.908 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:53.908 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:53.908 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:53.908 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:53.908 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:53.908 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:09:53.908 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:53.908 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:09:54.098 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:54.098 -07 000000627 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:54.098 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28879 150' command took 2 ms to send, 2263 ms to get response, and 2272 ms overall 17-03-18 00:09:54.098 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:09:54.098 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 5 at Y-position 62.3955078125, Z-Position -0.131392269531389. ------ 17-03-18 00:09:54.098 -07 000000382 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:54.098 -07 000000501 INF: Y Motor: MoveYZ optimized starting. Moving Y to 63.2326 mm, moving Z to -0.13812 mm 17-03-18 00:09:54.108 -07 000000501 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:54.108 -07 000000586 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:54.108 -07 000000382 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 4 ms to get response, and 5 ms overall 17-03-18 00:09:54.108 -07 000000382 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:09:54.108 -07 000000424 INF: Y Motor: Entering MoveAbsStepped (target = 101172 steps, sync = True, waitForSettling = True) 17-03-18 00:09:54.108 -07 000000424 INF: Y Motor: Moving to 63.2326 mm, started at 62.3955 mm, delta = 0.8371 mm 17-03-18 00:09:54.108 -07 000000586 INF: Z Motor: Entering MoveAbsStepped (DAC = 30172, sync = True, waitForSettling = False) 17-03-18 00:09:54.108 -07 000000586 INF: Z Motor: Moving to -0.138119 mm, started at -0.131392 mm, distance = -6.73 um 17-03-18 00:09:54.118 -07 000000586 INF: FPGA: Xmit ZDACW 30172\n [Z Motor] 17-03-18 00:09:54.118 -07 000000448 INF: FPGA: Recv ZDACW 17-03-18 00:09:54.128 -07 000000586 INF: FPGA: 'ZDACW 30172' command took 0 ms to send, 0 ms to get response, and 11 ms overall 17-03-18 00:09:54.128 -07 000000424 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:54.128 -07 000000448 INF: FPGA: Recv YRDPOS 127786 17-03-18 00:09:54.138 -07 000000424 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 23 ms overall 17-03-18 00:09:54.138 -07 000000586 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:54.138 -07 000000424 INF: Y Motor: Encoder = 127786 = motor step 99832.8 = 62.3955 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:54.138 -07 000000424 INF: Y Motor: Last commanded position = 62.4026 mm (99844.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:09:54.138 -07 000000424 INF: Y Motor: Moving 1339 steps-15 17-03-18 00:09:54.138 -07 000000012 INF: FPGA: Recv ZDACR 30172 17-03-18 00:09:54.148 -07 000000586 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:54.148 -07 000000586 INF: Z Motor: MoveAbsStepped took 31 ms (did NOT wait for settling yet)) 17-03-18 00:09:54.148 -07 000000424 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:09:54.288 -07 000000476 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 376 ms. 17-03-18 00:09:54.288 -07 000000476 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 377 ms. 17-03-18 00:09:54.288 -07 000000476 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:54.288 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 379 ms. 17-03-18 00:09:54.288 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 379 ms. 17-03-18 00:09:54.288 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:54.298 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:09:54.298 -07 000000476 INF: Camera: CopyActivePixFullImage end. Duration was 8 ms. 17-03-18 00:09:54.298 -07 000000476 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 389 ms. 17-03-18 00:09:54.298 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 391 ms. 17-03-18 00:09:54.308 -07 000000476 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:09:54.308 -07 000000382 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 198 ms to get response, and 202 ms overall 17-03-18 00:09:54.518 -07 000000476 INF: FPGA: Recv YMVTILE 17-03-18 00:09:54.528 -07 000000424 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 370 ms to get response, and 389 ms overall 17-03-18 00:09:54.568 -07 000000424 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:54.578 -07 000000561 INF: FPGA: Recv YRDPOS 129482 17-03-18 00:09:54.578 -07 000000424 INF: FPGA: 'YRDPOS' command took 0 ms to send, 2 ms to get response, and 9 ms overall 17-03-18 00:09:54.578 -07 000000424 INF: Y Motor: Encoder = 129482 = motor step 101157.8 = 63.2236 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:54.578 -07 000000424 INF: Y Motor: Position error before settling (table move) = -18 encoder counts (-0.009 mm) 17-03-18 00:09:54.578 -07 000000424 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 390 ms, distance = 0.837 mm, average velocity = 2.146 mm/sec 17-03-18 00:09:55.528 -07 000000424 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:55.528 -07 000000626 INF: FPGA: Recv YRDPOS 129483 17-03-18 00:09:55.538 -07 000000424 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.538 -07 000000424 INF: Y Motor: Encoder = 129483 = motor step 101158.6 = 63.2241 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:09:55.558 -07 000000424 INF: Y Motor: After move to 63.232609, error = -0.008488, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:09:55.558 -07 000000424 INF: Y Motor: Position error after settling (table move) = -17 encoder counts (-0.008 mm), Delta during settling = 1 counts (0.000 mm) 17-03-18 00:09:55.558 -07 000000424 INF: Y Motor: Moved to 63.2241 after 1449 ms (table move) 17-03-18 00:09:55.558 -07 000000501 INF: Z Motor: EndOfMoveAbsStepped: target = -0.138119 mm (DAC = 30172) 17-03-18 00:09:55.558 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.568 -07 000000476 INF: FPGA: Recv ZSGSRD 30794 17-03-18 00:09:55.568 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 2 ms to get response, and 2 ms overall 17-03-18 00:09:55.568 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.578 -07 000000586 INF: FPGA: Recv ZSGSRD 30794 17-03-18 00:09:55.578 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 2 ms to get response, and 10 ms overall 17-03-18 00:09:55.578 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.578 -07 000000627 INF: FPGA: Recv ZSGSRD 30796 17-03-18 00:09:55.588 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:09:55.588 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.588 -07 000000012 INF: FPGA: Recv ZSGSRD 30796 17-03-18 00:09:55.598 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.598 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.598 -07 000000012 INF: FPGA: Recv ZSGSRD 30790 17-03-18 00:09:55.608 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.608 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.608 -07 000000382 INF: FPGA: Recv ZSGSRD 30794 17-03-18 00:09:55.618 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.618 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.618 -07 000000012 INF: FPGA: Recv ZSGSRD 30789 17-03-18 00:09:55.628 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.628 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.628 -07 000000561 INF: FPGA: Recv ZSGSRD 30789 17-03-18 00:09:55.638 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.638 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.638 -07 000000561 INF: FPGA: Recv ZSGSRD 30791 17-03-18 00:09:55.648 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.648 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:55.648 -07 000000012 INF: FPGA: Recv ZSGSRD 30791 17-03-18 00:09:55.658 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:55.658 -07 000000501 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:55.658 -07 000000626 INF: FPGA: Recv ZPIEZORD 26533 17-03-18 00:09:55.668 -07 000000501 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:55.668 -07 000000501 INF: Z Motor: Piezo is near 36.7 V (nominally 0 to 100 V), expect near 46.0 V, since DAC is at 4.604 V. Piezo Error = -9.4 V. 17-03-18 00:09:55.668 -07 000000501 INF: Z Motor: ReadAdcAverage: Commanded Z = -138.1 um, Read 10 ADC samples: Avg = 30792 (-137.3 uncal um), Error = 0.8 uncal um, Median = 30792 (-137.3 uncal um), StdDev = 3 (0.0 uncal um), Range = 7 (0.0 uncal um), Min = 30789 (-137.3 uncal um), Max = 30796 (-137.4 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 3 (0.0 uncal um) 17-03-18 00:09:55.668 -07 000000501 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30792, posNoise = 4, negNoise = 3 17-03-18 00:09:55.668 -07 000000501 INF: Z Motor: After move to -0.138119, error = -0.000019, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:55.668 -07 000000501 INF: Z Motor: Settle wait time = 1450 ms, measure time = 101 ms (allowed 150 ms), total time = 1551 ms 17-03-18 00:09:55.668 -07 000000501 INF: Z Motor: Waiting extra 49 ms after reading position to reach expected total of 150 ms 17-03-18 00:09:55.718 -07 000000501 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1610 ms 17-03-18 00:09:55.718 -07 000000501 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:09:55.718 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:55.718 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:55.718 -07 000000626 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:55.718 -07 000000501 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:55.718 -07 000000586 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:09:55.718 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:55.718 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:55.718 -07 000000586 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:09:55.728 -07 000000586 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:09:55.728 -07 000000586 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:09:55.738 -07 000000586 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 10 ms. 17-03-18 00:09:55.738 -07 000000586 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 14 ms. 17-03-18 00:09:55.738 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:09:55.778 -07 000000627 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:09:56.078 -07 000000627 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:09:56.078 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:09:56.088 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:09:56.088 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:09:56.118 -07 000000561 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:09:56.118 -07 000000626 INF: FPGA: Recv CAMSNAP 17-03-18 00:09:56.118 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 378 ms to get response, and 384 ms overall 17-03-18 00:09:56.208 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 123 ms. 17-03-18 00:09:56.208 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:56.208 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:09:56.208 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 126 ms. 17-03-18 00:09:56.318 -07 000000382 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:56.318 -07 000000448 INF: Autofocus start 17-03-18 00:09:56.318 -07 000000448 INF: MeasureProfile start 17-03-18 00:09:56.318 -07 000000476 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:56.328 -07 000000382 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:09:56.328 -07 000000382 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:09:56.328 -07 000000448 INF: MeasureProfile done 17-03-18 00:09:56.328 -07 000000448 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:09:56.328 -07 000000448 INF: Autofocus finished 17-03-18 00:09:56.528 -07 000000424 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:09:56.528 -07 000000382 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:09:56.528 -07 000000610 INF: ------------ Scan Snapping tile 5 at Y-position 63.22412109375, Z-position -0.138137692280238. ---------- 17-03-18 00:09:56.528 -07 000000610 INF: Cycle 82 Tile 5 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:09:56.538 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28679, sync = True, waitForSettling = True) 17-03-18 00:09:56.538 -07 000000610 INF: Z Motor: Moving to -0.131284 mm, started at -0.138138 mm, distance = 6.85 um 17-03-18 00:09:56.538 -07 000000610 INF: FPGA: Xmit ZDACW 28679\n [Z Motor] 17-03-18 00:09:56.538 -07 000000627 INF: FPGA: Recv ZDACW 17-03-18 00:09:56.548 -07 000000610 INF: FPGA: 'ZDACW 28679' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.548 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:09:56.548 -07 000000627 INF: FPGA: Recv ZDACR 28679 17-03-18 00:09:56.558 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.558 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:09:56.708 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.708 -07 000000476 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:09:56.708 -07 000000476 INF: FPGA: Recv ZSGSRD 29728 17-03-18 00:09:56.718 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.738 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.738 -07 000000561 INF: FPGA: Recv ZSGSRD 29723 17-03-18 00:09:56.748 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:09:56.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.748 -07 000000424 INF: FPGA: Recv ZSGSRD 29731 17-03-18 00:09:56.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:56.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.768 -07 000000627 INF: FPGA: Recv ZSGSRD 29725 17-03-18 00:09:56.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:56.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.778 -07 000000012 INF: FPGA: Recv ZSGSRD 29725 17-03-18 00:09:56.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:09:56.798 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.798 -07 000000424 INF: FPGA: Recv ZSGSRD 29728 17-03-18 00:09:56.808 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:09:56.808 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.808 -07 000000424 INF: FPGA: Recv ZSGSRD 29725 17-03-18 00:09:56.818 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.818 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.818 -07 000000382 INF: FPGA: Recv ZSGSRD 29724 17-03-18 00:09:56.828 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.828 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.828 -07 000000382 INF: FPGA: Recv ZSGSRD 29731 17-03-18 00:09:56.838 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.838 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:09:56.838 -07 000000382 INF: FPGA: Recv ZSGSRD 29725 17-03-18 00:09:56.848 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.848 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:09:56.848 -07 000000382 INF: FPGA: Recv ZPIEZORD 25113 17-03-18 00:09:56.858 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:09:56.858 -07 000000610 INF: Z Motor: Piezo is near 33.6 V (nominally 0 to 100 V), expect near 43.8 V, since DAC is at 4.376 V. Piezo Error = -10.1 V. 17-03-18 00:09:56.858 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -131.3 um, Read 10 ADC samples: Avg = 29726 (-130.5 uncal um), Error = 0.8 uncal um, Median = 29725 (-130.5 uncal um), StdDev = 3 (0.0 uncal um), Range = 8 (0.1 uncal um), Min = 29723 (-130.5 uncal um), Max = 29731 (-130.5 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 3 (0.0 uncal um) 17-03-18 00:09:56.858 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29726, posNoise = 5, negNoise = 3 17-03-18 00:09:56.858 -07 000000610 INF: Z Motor: After move to -0.131284, error = -0.000005, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:09:56.858 -07 000000610 INF: Z Motor: MoveAbsStepped took 320 ms) 17-03-18 00:09:56.858 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:09:56.858 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:09:56.858 -07 000000561 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:09:56.858 -07 000000586 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:09:56.858 -07 000000476 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:09:56.858 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:09:56.858 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:09:56.858 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:09:56.858 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:09:56.858 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:09:56.858 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:09:56.858 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:09:56.858 -07 000000476 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:09:56.858 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:09:56.868 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:09:56.868 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:09:56.878 -07 000000476 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:09:56.878 -07 000000476 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:09:56.878 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:09:56.878 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:09:56.878 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28856 150\n [Camera] 17-03-18 00:09:57.078 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:09:57.138 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:09:57.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:09:58.118 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:09:58.118 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:09:58.118 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:58.118 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:58.118 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) start. 17-03-18 00:09:58.128 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:09:58.128 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:09:58.128 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:58.128 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:58.128 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 1113297000, 0x0, 418435064) start. 17-03-18 00:09:58.308 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28856 1422 17-03-18 00:09:58.458 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:09:58.498 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 1113297000, 0x0, 418435064) end. Duration was 377 ms. 17-03-18 00:09:58.498 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 377 ms. 17-03-18 00:09:58.498 -07 000000424 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:58.508 -07 000000424 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:58.508 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 1077874736, 0x0, 391868352) end. Duration was 387 ms. 17-03-18 00:09:58.508 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 388 ms. 17-03-18 00:09:58.508 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:58.508 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 388 ms. 17-03-18 00:09:58.518 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:58.518 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 391 ms. 17-03-18 00:09:58.968 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:09:58.968 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:09:58.968 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:09:58.968 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:09:58.968 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 1148719264, 0x0, 391868352) start. 17-03-18 00:09:58.968 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:09:58.968 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:09:58.968 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:09:58.968 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:09:58.968 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 2147487792, 0x0, 418435064) start. 17-03-18 00:09:59.158 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:09:59.158 -07 000000586 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:09:59.158 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28856 150' command took 2 ms to send, 2261 ms to get response, and 2272 ms overall 17-03-18 00:09:59.158 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:09:59.158 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 6 at Y-position 63.22412109375, Z-Position -0.131289482137121. ------ 17-03-18 00:09:59.158 -07 000000012 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:09:59.158 -07 000000586 INF: Y Motor: MoveYZ optimized starting. Moving Y to 64.0626 mm, moving Z to -0.13773 mm 17-03-18 00:09:59.158 -07 000000586 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:09:59.368 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 1148719264, 0x0, 391868352) end. Duration was 402 ms. 17-03-18 00:09:59.368 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:09:59.368 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:59.368 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 2147487792, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:09:59.368 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:09:59.368 -07 000000424 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:09:59.368 -07 000000424 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:09:59.378 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:09:59.378 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 410 ms. 17-03-18 00:09:59.378 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:09:59.388 -07 000000561 INF: Y Motor: Entering MoveAbsStepped (target = 102500 steps, sync = True, waitForSettling = True) 17-03-18 00:09:59.388 -07 000000561 INF: Y Motor: Moving to 64.0626 mm, started at 63.2241 mm, delta = 0.8385 mm 17-03-18 00:09:59.388 -07 000000561 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:09:59.388 -07 000000424 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:09:59.388 -07 000000012 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 224 ms to get response, and 226 ms overall 17-03-18 00:09:59.388 -07 000000424 INF: Z Motor: Entering MoveAbsStepped (DAC = 30087, sync = True, waitForSettling = False) 17-03-18 00:09:59.388 -07 000000424 INF: Z Motor: Moving to -0.137729 mm, started at -0.131289 mm, distance = -6.44 um 17-03-18 00:09:59.398 -07 000000424 INF: FPGA: Xmit ZDACW 30087\n [Z Motor] 17-03-18 00:09:59.408 -07 000000012 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:00.218 -07 000000608 INF: FPGA: Recv YRDPOS 129483 17-03-18 00:10:00.218 -07 000000608 INF: FPGA: Recv ZDACW 17-03-18 00:10:00.218 -07 000000608 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:00.218 -07 000000012 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 800 ms to get response, and 826 ms overall 17-03-18 00:10:00.218 -07 000000561 INF: FPGA: 'YRDPOS' command took 0 ms to send, 820 ms to get response, and 827 ms overall 17-03-18 00:10:00.218 -07 000000561 INF: Y Motor: Encoder = 129483 = motor step 101158.6 = 63.2241 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:00.218 -07 000000561 INF: Y Motor: Last commanded position = 63.2326 mm (101172.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:00.218 -07 000000561 INF: Y Motor: Moving 1342 steps-18 17-03-18 00:10:00.218 -07 000000424 INF: FPGA: 'ZDACW 30087' command took 0 ms to send, 810 ms to get response, and 824 ms overall 17-03-18 00:10:00.218 -07 000000424 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:00.218 -07 000000608 INF: FPGA: Recv ZDACR 30087 17-03-18 00:10:00.228 -07 000000424 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:00.228 -07 000000424 INF: Z Motor: MoveAbsStepped took 834 ms (did NOT wait for settling yet)) 17-03-18 00:10:00.228 -07 000000561 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:00.598 -07 000000012 INF: FPGA: Recv YMVTILE 17-03-18 00:10:00.598 -07 000000561 INF: FPGA: 'YMVTILE 1327' command took 1 ms to send, 369 ms to get response, and 387 ms overall 17-03-18 00:10:00.648 -07 000000561 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:00.648 -07 000000012 INF: FPGA: Recv YRDPOS 131189 17-03-18 00:10:00.658 -07 000000561 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:10:00.658 -07 000000561 INF: Y Motor: Encoder = 131189 = motor step 102491.4 = 64.0571 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:00.658 -07 000000561 INF: Y Motor: Position error before settling (table move) = -11 encoder counts (-0.005 mm) 17-03-18 00:10:00.658 -07 000000561 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 387 ms, distance = 0.838 mm, average velocity = 2.167 mm/sec 17-03-18 00:10:01.608 -07 000000561 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:01.608 -07 000000012 INF: FPGA: Recv YRDPOS 131189 17-03-18 00:10:01.618 -07 000000561 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.618 -07 000000561 INF: Y Motor: Encoder = 131189 = motor step 102491.4 = 64.0571 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:01.638 -07 000000561 INF: Y Motor: After move to 64.062607, error = -0.005478, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:01.638 -07 000000561 INF: Y Motor: Position error after settling (table move) = -11 encoder counts (-0.005 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:01.638 -07 000000561 INF: Y Motor: Moved to 64.0571 after 2251 ms (table move) 17-03-18 00:10:01.638 -07 000000586 INF: Z Motor: EndOfMoveAbsStepped: target = -0.137729 mm (DAC = 30087) 17-03-18 00:10:01.638 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.648 -07 000000561 INF: FPGA: Recv ZSGSRD 30732 17-03-18 00:10:01.648 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 2 ms overall 17-03-18 00:10:01.648 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.648 -07 000000561 INF: FPGA: Recv ZSGSRD 30732 17-03-18 00:10:01.658 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.658 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.658 -07 000000561 INF: FPGA: Recv ZSGSRD 30733 17-03-18 00:10:01.668 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.668 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.668 -07 000000561 INF: FPGA: Recv ZSGSRD 30728 17-03-18 00:10:01.678 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.678 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.678 -07 000000012 INF: FPGA: Recv ZSGSRD 30737 17-03-18 00:10:01.688 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.688 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.688 -07 000000561 INF: FPGA: Recv ZSGSRD 30725 17-03-18 00:10:01.698 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.698 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.698 -07 000000486 INF: FPGA: Recv ZSGSRD 30731 17-03-18 00:10:01.708 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:01.708 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.708 -07 000000478 INF: FPGA: Recv ZSGSRD 30731 17-03-18 00:10:01.718 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.718 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.718 -07 000000486 INF: FPGA: Recv ZSGSRD 30726 17-03-18 00:10:01.728 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.728 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:01.728 -07 000000478 INF: FPGA: Recv ZSGSRD 30734 17-03-18 00:10:01.738 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.738 -07 000000586 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:01.738 -07 000000012 INF: FPGA: Recv ZPIEZORD 26412 17-03-18 00:10:01.748 -07 000000586 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:01.748 -07 000000586 INF: Z Motor: Piezo is near 36.4 V (nominally 0 to 100 V), expect near 45.9 V, since DAC is at 4.591 V. Piezo Error = -9.5 V. 17-03-18 00:10:01.748 -07 000000586 INF: Z Motor: ReadAdcAverage: Commanded Z = -137.7 um, Read 10 ADC samples: Avg = 30731 (-136.9 uncal um), Error = 0.8 uncal um, Median = 30732 (-137.0 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 30725 (-136.9 uncal um), Max = 30737 (-137.0 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:10:01.748 -07 000000586 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30731, posNoise = 6, negNoise = 6 17-03-18 00:10:01.748 -07 000000586 INF: Z Motor: After move to -0.137729, error = -0.000016, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:01.748 -07 000000586 INF: Z Motor: Settle wait time = 2251 ms, measure time = 103 ms (allowed 150 ms), total time = 2354 ms 17-03-18 00:10:01.748 -07 000000586 INF: Z Motor: Waiting extra 47 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:01.798 -07 000000586 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 2631 ms 17-03-18 00:10:01.798 -07 000000586 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:01.798 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:01.798 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:01.798 -07 000000012 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:01.798 -07 000000486 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:01.798 -07 000000547 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:01.798 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:01.798 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:10:01.798 -07 000000547 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:01.798 -07 000000547 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:01.798 -07 000000547 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:01.808 -07 000000547 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 8 ms. 17-03-18 00:10:01.808 -07 000000547 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 12 ms. 17-03-18 00:10:01.818 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:01.858 -07 000000012 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:02.118 -07 000000478 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:10:02.138 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:02.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:02.158 -07 000000438 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:02.158 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:02.158 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:02.158 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1201016592, 0x0, 559484976) start. 17-03-18 00:10:02.198 -07 000000547 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:02.198 -07 000000486 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:02.198 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 376 ms to get response, and 385 ms overall 17-03-18 00:10:02.288 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1201016592, 0x0, 559484976) end. Duration was 124 ms. 17-03-18 00:10:02.288 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:02.288 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:10:02.288 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 127 ms. 17-03-18 00:10:02.298 -07 000000586 INF: Autofocus start 17-03-18 00:10:02.298 -07 000000586 INF: MeasureProfile start 17-03-18 00:10:02.298 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:02.298 -07 000000486 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:02.308 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:10:02.308 -07 000000512 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:02.308 -07 000000586 INF: MeasureProfile done 17-03-18 00:10:02.308 -07 000000586 INF: X=-16 Z correction [um]=-0.08128808 17-03-18 00:10:02.308 -07 000000586 INF: Autofocus finished 17-03-18 00:10:02.508 -07 000000478 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:02.508 -07 000000512 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:02.508 -07 000000610 INF: ------------ Scan Snapping tile 6 at Y-position 64.05712890625, Z-position -0.137745815339591. ---------- 17-03-18 00:10:02.508 -07 000000610 INF: Cycle 82 Tile 6 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:02.518 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28582, sync = True, waitForSettling = True) 17-03-18 00:10:02.518 -07 000000610 INF: Z Motor: Moving to -0.130840 mm, started at -0.137746 mm, distance = 6.91 um 17-03-18 00:10:02.518 -07 000000610 INF: FPGA: Xmit ZDACW 28582\n [Z Motor] 17-03-18 00:10:02.518 -07 000000478 INF: FPGA: Recv ZDACW 17-03-18 00:10:02.528 -07 000000610 INF: FPGA: 'ZDACW 28582' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:02.528 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:02.528 -07 000000512 INF: FPGA: Recv ZDACR 28582 17-03-18 00:10:02.538 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.538 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:02.688 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.688 -07 000000586 INF: FPGA: Recv ZSGSRD 29656 17-03-18 00:10:02.698 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:02.698 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.698 -07 000000478 INF: FPGA: Recv ZSGSRD 29657 17-03-18 00:10:02.708 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.708 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.708 -07 000000486 INF: FPGA: Recv ZSGSRD 29653 17-03-18 00:10:02.718 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.718 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.718 -07 000000561 INF: FPGA: Recv ZSGSRD 29658 17-03-18 00:10:02.728 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.728 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.728 -07 000000512 INF: FPGA: Recv ZSGSRD 29657 17-03-18 00:10:02.738 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.738 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.738 -07 000000547 INF: FPGA: Recv ZSGSRD 29655 17-03-18 00:10:02.748 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.748 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.748 -07 000000438 INF: FPGA: Recv ZSGSRD 29658 17-03-18 00:10:02.758 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.758 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.758 -07 000000547 INF: FPGA: Recv ZSGSRD 29658 17-03-18 00:10:02.768 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.768 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.768 -07 000000486 INF: FPGA: Recv ZSGSRD 29658 17-03-18 00:10:02.778 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.778 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:02.778 -07 000000478 INF: FPGA: Recv ZSGSRD 29657 17-03-18 00:10:02.788 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.788 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:02.788 -07 000000586 INF: FPGA: Recv ZPIEZORD 25036 17-03-18 00:10:02.798 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:02.798 -07 000000610 INF: Z Motor: Piezo is near 33.5 V (nominally 0 to 100 V), expect near 43.6 V, since DAC is at 4.361 V. Piezo Error = -10.1 V. 17-03-18 00:10:02.798 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -130.8 um, Read 10 ADC samples: Avg = 29657 (-130.1 uncal um), Error = 0.8 uncal um, Median = 29657 (-130.1 uncal um), StdDev = 2 (0.0 uncal um), Range = 5 (0.0 uncal um), Min = 29653 (-130.0 uncal um), Max = 29658 (-130.1 uncal um), Pos Noise = 1 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:10:02.798 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29657, posNoise = 1, negNoise = 4 17-03-18 00:10:02.798 -07 000000610 INF: Z Motor: After move to -0.130840, error = -0.000006, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:02.798 -07 000000610 INF: Z Motor: MoveAbsStepped took 279 ms) 17-03-18 00:10:02.798 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:02.798 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:02.798 -07 000000478 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:02.798 -07 000000012 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:02.798 -07 000000586 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:02.798 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:02.798 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:02.798 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:02.798 -07 000000586 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:10:02.798 -07 000000586 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:02.798 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:02.798 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:02.808 -07 000000586 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:10:02.808 -07 000000586 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:02.808 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:02.808 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:02.818 -07 000000586 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:10:02.818 -07 000000586 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:10:02.818 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:10:02.818 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:02.828 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28759 150\n [Camera] 17-03-18 00:10:03.028 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:04.058 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:04.068 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:04.068 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:04.068 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:04.068 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2187315360, 0x0, 391868352) start. 17-03-18 00:10:04.068 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:04.068 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:04.068 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:04.068 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:04.068 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2222737624, 0x0, 418435064) start. 17-03-18 00:10:04.468 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2187315360, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:10:04.468 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:10:04.468 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:04.468 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:04.468 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:10:04.468 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2222737624, 0x0, 418435064) end. Duration was 401 ms. 17-03-18 00:10:04.468 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 401 ms. 17-03-18 00:10:04.468 -07 000000012 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:04.468 -07 000000012 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:04.478 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28759 1422 17-03-18 00:10:04.478 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:04.478 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 413 ms. 17-03-18 00:10:04.908 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:04.908 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:04.908 -07 000000012 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:04.908 -07 000000012 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:04.908 -07 000000012 INF: Camera: sBCa_ReadImage(785074288, 2281705520, 0x0, 391868352) start. 17-03-18 00:10:04.908 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:04.908 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:04.908 -07 000000512 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:04.908 -07 000000512 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:04.908 -07 000000512 INF: Camera: sBCa_ReadImage(785074704, 2317127784, 0x0, 418435064) start. 17-03-18 00:10:05.288 -07 000000012 INF: Camera: sBCa_ReadImage(785074288, 2281705520, 0x0, 391868352) end. Duration was 381 ms. 17-03-18 00:10:05.288 -07 000000012 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 381 ms. 17-03-18 00:10:05.288 -07 000000012 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:05.288 -07 000000512 INF: Camera: sBCa_ReadImage(785074704, 2317127784, 0x0, 418435064) end. Duration was 380 ms. 17-03-18 00:10:05.288 -07 000000512 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 380 ms. 17-03-18 00:10:05.288 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:05.298 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:05.298 -07 000000012 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:10:05.298 -07 000000012 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 389 ms. 17-03-18 00:10:05.298 -07 000000512 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 391 ms. 17-03-18 00:10:05.308 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:05.308 -07 000000012 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:05.308 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28759 150' command took 2 ms to send, 2472 ms to get response, and 2480 ms overall 17-03-18 00:10:05.308 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:05.308 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 7 at Y-position 64.05712890625, Z-Position -0.13084621149934. ------ 17-03-18 00:10:05.308 -07 000000012 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:05.308 -07 000000512 INF: Y Motor: MoveYZ optimized starting. Moving Y to 64.8926 mm, moving Z to -0.13742 mm 17-03-18 00:10:05.308 -07 000000512 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:06.158 -07 000000502 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:06.158 -07 000000012 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 840 ms to get response, and 842 ms overall 17-03-18 00:10:06.158 -07 000000012 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:06.158 -07 000000502 INF: Y Motor: Entering MoveAbsStepped (target = 103828 steps, sync = True, waitForSettling = True) 17-03-18 00:10:06.158 -07 000000502 INF: Y Motor: Moving to 64.8926 mm, started at 64.0571 mm, delta = 0.8355 mm 17-03-18 00:10:06.168 -07 000000502 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:06.688 -07 000000448 INF: FPGA: Recv YRDPOS 131189 17-03-18 00:10:06.688 -07 000000448 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:06.688 -07 000000012 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 520 ms to get response, and 529 ms overall 17-03-18 00:10:06.688 -07 000000502 INF: FPGA: 'YRDPOS' command took 0 ms to send, 510 ms to get response, and 528 ms overall 17-03-18 00:10:06.688 -07 000000502 INF: Y Motor: Encoder = 131189 = motor step 102491.4 = 64.0571 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:06.688 -07 000000502 INF: Y Motor: Last commanded position = 64.0626 mm (102500.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:06.688 -07 000000502 INF: Y Motor: Moving 1337 steps-11 17-03-18 00:10:06.688 -07 000000502 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:06.688 -07 000000448 INF: Z Motor: Entering MoveAbsStepped (DAC = 30020, sync = True, waitForSettling = False) 17-03-18 00:10:06.688 -07 000000448 INF: Z Motor: Moving to -0.137423 mm, started at -0.130846 mm, distance = -6.58 um 17-03-18 00:10:06.698 -07 000000448 INF: FPGA: Xmit ZDACW 30020\n [Z Motor] 17-03-18 00:10:06.698 -07 000000012 INF: FPGA: Recv ZDACW 17-03-18 00:10:06.708 -07 000000448 INF: FPGA: 'ZDACW 30020' command took 0 ms to send, 0 ms to get response, and 18 ms overall 17-03-18 00:10:06.708 -07 000000448 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:06.708 -07 000000012 INF: FPGA: Recv ZDACR 30020 17-03-18 00:10:06.718 -07 000000448 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:06.718 -07 000000448 INF: Z Motor: MoveAbsStepped took 28 ms (did NOT wait for settling yet)) 17-03-18 00:10:07.058 -07 000000448 INF: FPGA: Recv YMVTILE 17-03-18 00:10:07.058 -07 000000502 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 369 ms to get response, and 378 ms overall 17-03-18 00:10:07.128 -07 000000502 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:07.128 -07 000000382 INF: FPGA: Recv YRDPOS 132872 17-03-18 00:10:07.138 -07 000000502 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:10:07.138 -07 000000502 INF: Y Motor: Encoder = 132872 = motor step 103806.3 = 64.8789 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:07.138 -07 000000502 INF: Y Motor: Position error before settling (table move) = -28 encoder counts (-0.014 mm) 17-03-18 00:10:07.138 -07 000000502 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 378 ms, distance = 0.835 mm, average velocity = 2.210 mm/sec 17-03-18 00:10:07.138 -07 000000626 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:07.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:07.478 -07 000000626 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:10:08.088 -07 000000502 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:08.088 -07 000000627 INF: FPGA: Recv YRDPOS 132872 17-03-18 00:10:08.098 -07 000000502 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.098 -07 000000502 INF: Y Motor: Encoder = 132872 = motor step 103806.3 = 64.8789 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:08.118 -07 000000502 INF: Y Motor: After move to 64.892609, error = -0.013702, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:08.118 -07 000000502 INF: Y Motor: Position error after settling (table move) = -28 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:08.118 -07 000000502 INF: Y Motor: Moved to 64.8789 after 1964 ms (table move) 17-03-18 00:10:08.128 -07 000000512 INF: Z Motor: EndOfMoveAbsStepped: target = -0.137423 mm (DAC = 30020) 17-03-18 00:10:08.128 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.128 -07 000000382 INF: FPGA: Recv ZSGSRD 30686 17-03-18 00:10:08.138 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.138 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.138 -07 000000626 INF: FPGA: Recv ZSGSRD 30679 17-03-18 00:10:08.148 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.148 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.148 -07 000000448 INF: FPGA: Recv ZSGSRD 30675 17-03-18 00:10:08.158 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.158 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.158 -07 000000626 INF: FPGA: Recv ZSGSRD 30679 17-03-18 00:10:08.168 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.168 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.168 -07 000000382 INF: FPGA: Recv ZSGSRD 30678 17-03-18 00:10:08.178 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.178 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.178 -07 000000627 INF: FPGA: Recv ZSGSRD 30684 17-03-18 00:10:08.188 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.188 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.188 -07 000000476 INF: FPGA: Recv ZSGSRD 30687 17-03-18 00:10:08.198 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.198 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.198 -07 000000627 INF: FPGA: Recv ZSGSRD 30683 17-03-18 00:10:08.208 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.208 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.208 -07 000000012 INF: FPGA: Recv ZSGSRD 30687 17-03-18 00:10:08.218 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.218 -07 000000512 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:08.218 -07 000000502 INF: FPGA: Recv ZSGSRD 30678 17-03-18 00:10:08.228 -07 000000512 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.228 -07 000000512 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:08.228 -07 000000501 INF: FPGA: Recv ZPIEZORD 26410 17-03-18 00:10:08.238 -07 000000512 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:08.238 -07 000000512 INF: Z Motor: Piezo is near 36.4 V (nominally 0 to 100 V), expect near 45.8 V, since DAC is at 4.581 V. Piezo Error = -9.4 V. 17-03-18 00:10:08.238 -07 000000512 INF: Z Motor: ReadAdcAverage: Commanded Z = -137.4 um, Read 10 ADC samples: Avg = 30682 (-136.6 uncal um), Error = 0.8 uncal um, Median = 30681 (-136.6 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 30675 (-136.6 uncal um), Max = 30687 (-136.7 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:10:08.238 -07 000000512 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30682, posNoise = 5, negNoise = 7 17-03-18 00:10:08.238 -07 000000512 INF: Z Motor: After move to -0.137423, error = -0.000008, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:08.238 -07 000000512 INF: Z Motor: Settle wait time = 1438 ms, measure time = 110 ms (allowed 150 ms), total time = 1548 ms 17-03-18 00:10:08.238 -07 000000512 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:08.278 -07 000000512 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 2961 ms 17-03-18 00:10:08.278 -07 000000512 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:08.278 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:08.278 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:08.278 -07 000000512 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:08.278 -07 000000501 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:08.278 -07 000000476 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:08.278 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:08.288 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:08.288 -07 000000476 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:08.288 -07 000000476 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:08.288 -07 000000476 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:08.298 -07 000000476 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 9 ms. 17-03-18 00:10:08.298 -07 000000476 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 14 ms. 17-03-18 00:10:08.298 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:08.348 -07 000000502 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:08.648 -07 000000502 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:08.648 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:08.648 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:08.648 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2352550048, 0x0, 559484976) start. 17-03-18 00:10:08.678 -07 000000626 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:08.688 -07 000000012 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:08.688 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 380 ms to get response, and 384 ms overall 17-03-18 00:10:08.778 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2352550048, 0x0, 559484976) end. Duration was 129 ms. 17-03-18 00:10:08.778 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:08.778 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:10:08.778 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 131 ms. 17-03-18 00:10:08.788 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:08.788 -07 000000424 INF: Autofocus start 17-03-18 00:10:08.788 -07 000000424 INF: MeasureProfile start 17-03-18 00:10:08.788 -07 000000448 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:08.798 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:08.798 -07 000000512 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:08.798 -07 000000424 INF: MeasureProfile done 17-03-18 00:10:08.798 -07 000000424 INF: X=-48 Z correction [um]=-0.2370583 17-03-18 00:10:08.798 -07 000000424 INF: Autofocus finished 17-03-18 00:10:08.998 -07 000000476 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:08.998 -07 000000512 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:08.998 -07 000000610 INF: ------------ Scan Snapping tile 7 at Y-position 64.87890625, Z-position -0.137431028944645. ---------- 17-03-18 00:10:09.008 -07 000000610 INF: Cycle 82 Tile 7 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:09.008 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28481, sync = True, waitForSettling = True) 17-03-18 00:10:09.008 -07 000000610 INF: Z Motor: Moving to -0.130378 mm, started at -0.137431 mm, distance = 7.05 um 17-03-18 00:10:09.008 -07 000000610 INF: FPGA: Xmit ZDACW 28481\n [Z Motor] 17-03-18 00:10:09.008 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:10:09.018 -07 000000610 INF: FPGA: 'ZDACW 28481' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:09.018 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:09.018 -07 000000382 INF: FPGA: Recv ZDACR 28481 17-03-18 00:10:09.028 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.028 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:09.178 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.178 -07 000000626 INF: FPGA: Recv ZSGSRD 29583 17-03-18 00:10:09.188 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:09.188 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.188 -07 000000626 INF: FPGA: Recv ZSGSRD 29588 17-03-18 00:10:09.198 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.198 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.198 -07 000000626 INF: FPGA: Recv ZSGSRD 29586 17-03-18 00:10:09.208 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.208 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.208 -07 000000502 INF: FPGA: Recv ZSGSRD 29585 17-03-18 00:10:09.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.218 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.218 -07 000000501 INF: FPGA: Recv ZSGSRD 29588 17-03-18 00:10:09.228 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.228 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.228 -07 000000502 INF: FPGA: Recv ZSGSRD 29590 17-03-18 00:10:09.238 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.238 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.238 -07 000000501 INF: FPGA: Recv ZSGSRD 29583 17-03-18 00:10:09.248 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.248 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.248 -07 000000501 INF: FPGA: Recv ZSGSRD 29584 17-03-18 00:10:09.258 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.258 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.258 -07 000000501 INF: FPGA: Recv ZSGSRD 29584 17-03-18 00:10:09.268 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.268 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:09.268 -07 000000627 INF: FPGA: Recv ZSGSRD 29579 17-03-18 00:10:09.278 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.278 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:09.278 -07 000000627 INF: FPGA: Recv ZPIEZORD 24946 17-03-18 00:10:09.288 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:09.288 -07 000000610 INF: Z Motor: Piezo is near 33.3 V (nominally 0 to 100 V), expect near 43.5 V, since DAC is at 4.346 V. Piezo Error = -10.2 V. 17-03-18 00:10:09.288 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -130.4 um, Read 10 ADC samples: Avg = 29585 (-129.6 uncal um), Error = 0.8 uncal um, Median = 29584 (-129.6 uncal um), StdDev = 3 (0.0 uncal um), Range = 11 (0.1 uncal um), Min = 29579 (-129.6 uncal um), Max = 29590 (-129.6 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:10:09.288 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29585, posNoise = 5, negNoise = 6 17-03-18 00:10:09.288 -07 000000610 INF: Z Motor: After move to -0.130378, error = -0.000006, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:09.288 -07 000000610 INF: Z Motor: MoveAbsStepped took 278 ms) 17-03-18 00:10:09.288 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:09.288 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:09.288 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:09.288 -07 000000501 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:09.288 -07 000000501 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:09.288 -07 000000012 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:09.288 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:09.288 -07 000000512 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:09.288 -07 000000501 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:09.288 -07 000000501 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:09.288 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:09.288 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:09.298 -07 000000501 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:09.298 -07 000000501 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:09.298 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:10:09.298 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:09.308 -07 000000501 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 11 ms. 17-03-18 00:10:09.308 -07 000000501 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 16 ms. 17-03-18 00:10:09.308 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 13 ms. 17-03-18 00:10:09.308 -07 000000012 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 18 ms. 17-03-18 00:10:09.308 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28658 150\n [Camera] 17-03-18 00:10:09.508 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:10.548 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:10.548 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:10.548 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:10.548 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:10.548 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2367226128, 0x0, 391868352) start. 17-03-18 00:10:10.548 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:10.548 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:10.548 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:10.548 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:10.548 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 2415923248, 0x0, 418435064) start. 17-03-18 00:10:10.738 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28658 1422 17-03-18 00:10:10.888 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:10.928 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2367226128, 0x0, 391868352) end. Duration was 376 ms. 17-03-18 00:10:10.928 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 376 ms. 17-03-18 00:10:10.928 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:10.928 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 2415923248, 0x0, 418435064) end. Duration was 374 ms. 17-03-18 00:10:10.928 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 374 ms. 17-03-18 00:10:10.928 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:10.928 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 5 ms. 17-03-18 00:10:10.928 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 382 ms. 17-03-18 00:10:10.928 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 5 ms. 17-03-18 00:10:10.938 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 387 ms. 17-03-18 00:10:11.388 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:11.388 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:11.388 -07 000000501 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:11.388 -07 000000501 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:11.388 -07 000000501 INF: Camera: sBCa_ReadImage(785074288, 2451345512, 0x0, 391868352) start. 17-03-18 00:10:11.398 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:11.398 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:11.398 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:11.398 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:11.398 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2503642840, 0x0, 418435064) start. 17-03-18 00:10:11.578 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:11.578 -07 000000512 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:11.578 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28658 150' command took 2 ms to send, 2259 ms to get response, and 2272 ms overall 17-03-18 00:10:11.588 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:11.588 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 8 at Y-position 64.87890625, Z-Position -0.130383668225133. ------ 17-03-18 00:10:11.588 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:11.588 -07 000000627 INF: Y Motor: MoveYZ optimized starting. Moving Y to 65.7226 mm, moving Z to -0.13702 mm 17-03-18 00:10:11.588 -07 000000627 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:11.798 -07 000000501 INF: Camera: sBCa_ReadImage(785074288, 2451345512, 0x0, 391868352) end. Duration was 401 ms. 17-03-18 00:10:11.798 -07 000000501 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:10:11.798 -07 000000501 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:11.798 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2503642840, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:10:11.798 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:10:11.798 -07 000000012 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:11.798 -07 000000012 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:11.798 -07 000000501 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:10:11.798 -07 000000501 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 410 ms. 17-03-18 00:10:11.808 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:10:11.808 -07 000000501 INF: Y Motor: Entering MoveAbsStepped (target = 105156 steps, sync = True, waitForSettling = True) 17-03-18 00:10:11.808 -07 000000501 INF: Y Motor: Moving to 65.7226 mm, started at 64.8789 mm, delta = 0.8437 mm 17-03-18 00:10:11.808 -07 000000501 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:11.818 -07 000000012 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:11.818 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 221 ms to get response, and 225 ms overall 17-03-18 00:10:11.818 -07 000000512 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:11.818 -07 000000012 INF: Z Motor: Entering MoveAbsStepped (DAC = 29932, sync = True, waitForSettling = False) 17-03-18 00:10:11.818 -07 000000012 INF: Z Motor: Moving to -0.137020 mm, started at -0.130384 mm, distance = -6.64 um 17-03-18 00:10:11.828 -07 000000012 INF: FPGA: Xmit ZDACW 29932\n [Z Motor] 17-03-18 00:10:12.718 -07 000000608 INF: FPGA: Recv YRDPOS 132872 17-03-18 00:10:12.718 -07 000000608 INF: FPGA: Recv ZDACW 17-03-18 00:10:12.718 -07 000000608 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:12.718 -07 000000608 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:12.718 -07 000000012 INF: FPGA: 'ZDACW 29932' command took 0 ms to send, 880 ms to get response, and 897 ms overall 17-03-18 00:10:12.718 -07 000000012 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:12.718 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:12.718 -07 000000501 INF: FPGA: 'YRDPOS' command took 0 ms to send, 900 ms to get response, and 901 ms overall 17-03-18 00:10:12.718 -07 000000501 INF: Y Motor: Encoder = 132872 = motor step 103806.3 = 64.8789 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:12.718 -07 000000501 INF: Y Motor: Last commanded position = 64.8926 mm (103828.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:12.718 -07 000000501 INF: Y Motor: Moving 1350 steps-28 17-03-18 00:10:12.718 -07 000000512 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 891 ms to get response, and 898 ms overall 17-03-18 00:10:12.718 -07 000000608 INF: FPGA: Recv ZDACR 29932 17-03-18 00:10:12.728 -07 000000012 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:12.728 -07 000000501 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:12.728 -07 000000012 INF: Z Motor: MoveAbsStepped took 906 ms (did NOT wait for settling yet)) 17-03-18 00:10:13.098 -07 000000512 INF: FPGA: Recv YMVTILE 17-03-18 00:10:13.098 -07 000000501 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 385 ms overall 17-03-18 00:10:13.148 -07 000000501 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:13.148 -07 000000569 INF: FPGA: Recv YRDPOS 134582 17-03-18 00:10:13.158 -07 000000501 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:13.158 -07 000000501 INF: Y Motor: Encoder = 134582 = motor step 105142.2 = 65.7139 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:13.158 -07 000000501 INF: Y Motor: Position error before settling (table move) = -18 encoder counts (-0.009 mm) 17-03-18 00:10:13.158 -07 000000501 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 385 ms, distance = 0.844 mm, average velocity = 2.191 mm/sec 17-03-18 00:10:13.338 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:10:14.108 -07 000000501 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:14.108 -07 000000547 INF: FPGA: Recv YRDPOS 134582 17-03-18 00:10:14.118 -07 000000501 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:14.118 -07 000000501 INF: Y Motor: Encoder = 134582 = motor step 105142.2 = 65.7139 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:14.148 -07 000000501 INF: Y Motor: After move to 65.722610, error = -0.008743, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:14.148 -07 000000501 INF: Y Motor: Position error after settling (table move) = -18 encoder counts (-0.009 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:14.148 -07 000000501 INF: Y Motor: Moved to 65.7139 after 2331 ms (table move) 17-03-18 00:10:14.148 -07 000000627 INF: Z Motor: EndOfMoveAbsStepped: target = -0.137020 mm (DAC = 29932) 17-03-18 00:10:14.148 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.148 -07 000000547 INF: FPGA: Recv ZSGSRD 30624 17-03-18 00:10:14.158 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:10:14.158 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.158 -07 000000586 INF: FPGA: Recv ZSGSRD 30619 17-03-18 00:10:14.168 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.168 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.168 -07 000000569 INF: FPGA: Recv ZSGSRD 30624 17-03-18 00:10:14.178 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.178 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.178 -07 000000501 INF: FPGA: Recv ZSGSRD 30618 17-03-18 00:10:14.188 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.188 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.188 -07 000000012 INF: FPGA: Recv ZSGSRD 30619 17-03-18 00:10:14.198 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.198 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.198 -07 000000608 INF: FPGA: Recv ZSGSRD 30620 17-03-18 00:10:14.208 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.208 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.208 -07 000000012 INF: FPGA: Recv ZSGSRD 30615 17-03-18 00:10:14.218 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.218 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.218 -07 000000501 INF: FPGA: Recv ZSGSRD 30618 17-03-18 00:10:14.228 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.228 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.228 -07 000000012 INF: FPGA: Recv ZSGSRD 30616 17-03-18 00:10:14.238 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.238 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:14.238 -07 000000501 INF: FPGA: Recv ZSGSRD 30618 17-03-18 00:10:14.248 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.248 -07 000000627 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:14.248 -07 000000012 INF: FPGA: Recv ZPIEZORD 26366 17-03-18 00:10:14.258 -07 000000627 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:14.258 -07 000000627 INF: Z Motor: Piezo is near 36.3 V (nominally 0 to 100 V), expect near 45.7 V, since DAC is at 4.567 V. Piezo Error = -9.3 V. 17-03-18 00:10:14.258 -07 000000627 INF: Z Motor: ReadAdcAverage: Commanded Z = -137.0 um, Read 10 ADC samples: Avg = 30619 (-136.2 uncal um), Error = 0.8 uncal um, Median = 30618 (-136.2 uncal um), StdDev = 3 (0.0 uncal um), Range = 9 (0.1 uncal um), Min = 30615 (-136.2 uncal um), Max = 30624 (-136.3 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:10:14.258 -07 000000627 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30619, posNoise = 5, negNoise = 4 17-03-18 00:10:14.258 -07 000000627 INF: Z Motor: After move to -0.137020, error = -0.000006, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:14.258 -07 000000627 INF: Z Motor: Settle wait time = 2332 ms, measure time = 105 ms (allowed 150 ms), total time = 2437 ms 17-03-18 00:10:14.258 -07 000000627 INF: Z Motor: Waiting extra 45 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:14.308 -07 000000627 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 2713 ms 17-03-18 00:10:14.308 -07 000000627 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:14.308 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:14.308 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:14.308 -07 000000501 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:14.308 -07 000000569 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:14.308 -07 000000012 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:14.308 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:14.308 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:14.308 -07 000000012 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:14.318 -07 000000012 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:14.318 -07 000000012 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:14.328 -07 000000012 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 10 ms. 17-03-18 00:10:14.328 -07 000000012 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 15 ms. 17-03-18 00:10:14.328 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:14.378 -07 000000501 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:14.668 -07 000000482 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:14.678 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:14.678 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:14.678 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2402648392, 0x0, 559484976) start. 17-03-18 00:10:14.708 -07 000000586 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:14.718 -07 000000012 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:14.718 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 380 ms to get response, and 384 ms overall 17-03-18 00:10:14.798 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2402648392, 0x0, 559484976) end. Duration was 127 ms. 17-03-18 00:10:14.798 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:14.808 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:10:14.808 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 129 ms. 17-03-18 00:10:14.818 -07 000000012 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:14.818 -07 000000501 INF: Autofocus start 17-03-18 00:10:14.818 -07 000000501 INF: MeasureProfile start 17-03-18 00:10:14.818 -07 000000569 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:14.828 -07 000000012 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:10:14.828 -07 000000012 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:14.828 -07 000000501 INF: MeasureProfile done 17-03-18 00:10:14.828 -07 000000501 INF: X=16 Z correction [um]=0.0530179 17-03-18 00:10:14.828 -07 000000501 INF: Autofocus finished 17-03-18 00:10:15.028 -07 000000547 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:15.028 -07 000000012 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:15.028 -07 000000610 INF: ------------ Scan Snapping tile 8 at Y-position 65.7138671875, Z-position -0.137026303579714. ---------- 17-03-18 00:10:15.028 -07 000000610 INF: Cycle 82 Tile 8 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:15.038 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28456, sync = True, waitForSettling = True) 17-03-18 00:10:15.038 -07 000000610 INF: Z Motor: Moving to -0.130263 mm, started at -0.137026 mm, distance = 6.76 um 17-03-18 00:10:15.038 -07 000000610 INF: FPGA: Xmit ZDACW 28456\n [Z Motor] 17-03-18 00:10:15.038 -07 000000501 INF: FPGA: Recv ZDACW 17-03-18 00:10:15.048 -07 000000610 INF: FPGA: 'ZDACW 28456' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:15.048 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:15.048 -07 000000586 INF: FPGA: Recv ZDACR 28456 17-03-18 00:10:15.058 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.058 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:15.208 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.208 -07 000000608 INF: FPGA: Recv ZSGSRD 29566 17-03-18 00:10:15.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:15.218 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.218 -07 000000586 INF: FPGA: Recv ZSGSRD 29572 17-03-18 00:10:15.228 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:10:15.228 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.228 -07 000000608 INF: FPGA: Recv ZSGSRD 29568 17-03-18 00:10:15.238 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.238 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.238 -07 000000608 INF: FPGA: Recv ZSGSRD 29564 17-03-18 00:10:15.248 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.248 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.248 -07 000000627 INF: FPGA: Recv ZSGSRD 29565 17-03-18 00:10:15.258 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.258 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.258 -07 000000512 INF: FPGA: Recv ZSGSRD 29572 17-03-18 00:10:15.268 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.268 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.268 -07 000000012 INF: FPGA: Recv ZSGSRD 29564 17-03-18 00:10:15.278 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.278 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.278 -07 000000482 INF: FPGA: Recv ZSGSRD 29560 17-03-18 00:10:15.288 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.288 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.288 -07 000000586 INF: FPGA: Recv ZSGSRD 29568 17-03-18 00:10:15.298 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.298 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:15.298 -07 000000608 INF: FPGA: Recv ZSGSRD 29568 17-03-18 00:10:15.308 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.308 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:15.308 -07 000000012 INF: FPGA: Recv ZPIEZORD 24994 17-03-18 00:10:15.318 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:15.318 -07 000000610 INF: Z Motor: Piezo is near 33.4 V (nominally 0 to 100 V), expect near 43.4 V, since DAC is at 4.342 V. Piezo Error = -10.0 V. 17-03-18 00:10:15.318 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -130.3 um, Read 10 ADC samples: Avg = 29567 (-129.5 uncal um), Error = 0.8 uncal um, Median = 29567 (-129.5 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 29560 (-129.4 uncal um), Max = 29572 (-129.5 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 7 (0.0 uncal um) 17-03-18 00:10:15.318 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29567, posNoise = 5, negNoise = 7 17-03-18 00:10:15.318 -07 000000610 INF: Z Motor: After move to -0.130263, error = -0.000005, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:15.318 -07 000000610 INF: Z Motor: MoveAbsStepped took 279 ms) 17-03-18 00:10:15.318 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:15.318 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:15.318 -07 000000512 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:15.318 -07 000000482 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:15.318 -07 000000547 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:15.318 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:15.318 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:15.318 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:15.318 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:15.318 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:15.318 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:15.318 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:15.328 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:10:15.328 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:15.328 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:15.328 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:15.338 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:10:15.338 -07 000000547 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:15.338 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:10:15.338 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:15.348 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28633 150\n [Camera] 17-03-18 00:10:15.548 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:16.588 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1232 17-03-18 00:10:16.588 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1232 17-03-18 00:10:16.588 -07 000000482 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:16.588 -07 000000482 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:16.588 -07 000000482 INF: Camera: sBCa_ReadImage(785074288, 2550140976, 0x0, 391868352) start. 17-03-18 00:10:16.588 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1232 17-03-18 00:10:16.588 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1232 17-03-18 00:10:16.588 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:16.588 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:16.588 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2585563240, 0x0, 418435064) start. 17-03-18 00:10:16.778 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28633 1422 17-03-18 00:10:16.928 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:16.978 -07 000000482 INF: Camera: sBCa_ReadImage(785074288, 2550140976, 0x0, 391868352) end. Duration was 393 ms. 17-03-18 00:10:16.978 -07 000000482 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 393 ms. 17-03-18 00:10:16.978 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:16.978 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 2585563240, 0x0, 418435064) end. Duration was 392 ms. 17-03-18 00:10:16.978 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 393 ms. 17-03-18 00:10:16.978 -07 000000012 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:16.988 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 5 ms. 17-03-18 00:10:16.988 -07 000000482 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:10:16.988 -07 000000012 INF: Camera: CopyActivePixFullImage end. Duration was 5 ms. 17-03-18 00:10:16.988 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 405 ms. 17-03-18 00:10:17.138 -07 000000627 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:17.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:17.428 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:17.428 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:17.428 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:17.428 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:17.428 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 2620985504, 0x0, 391868352) start. 17-03-18 00:10:17.428 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:17.428 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:17.428 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:17.578 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:17.578 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 1389170736, 0x0, 418435064) start. 17-03-18 00:10:17.618 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:17.618 -07 000000482 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:17.618 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28633 150' command took 2 ms to send, 2262 ms to get response, and 2272 ms overall 17-03-18 00:10:17.618 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:17.618 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 9 at Y-position 65.7138671875, Z-Position -0.130268032406581. ------ 17-03-18 00:10:17.618 -07 000000012 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:17.618 -07 000000627 INF: Y Motor: MoveYZ optimized starting. Moving Y to 66.5526 mm, moving Z to -0.13657 mm 17-03-18 00:10:17.618 -07 000000627 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:17.628 -07 000000482 INF: Y Motor: Entering MoveAbsStepped (target = 106484 steps, sync = True, waitForSettling = True) 17-03-18 00:10:17.628 -07 000000482 INF: Y Motor: Moving to 66.5526 mm, started at 65.7139 mm, delta = 0.8387 mm 17-03-18 00:10:17.628 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:17.828 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 2620985504, 0x0, 391868352) end. Duration was 402 ms. 17-03-18 00:10:17.828 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:10:17.828 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:17.838 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 6 ms. 17-03-18 00:10:17.838 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 409 ms. 17-03-18 00:10:17.848 -07 000000561 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:17.848 -07 000000561 INF: FPGA: Recv YRDPOS 134583 17-03-18 00:10:17.848 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 210 ms to get response, and 218 ms overall 17-03-18 00:10:17.848 -07 000000482 INF: Y Motor: Encoder = 134583 = motor step 105143.0 = 65.7144 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:17.848 -07 000000012 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 220 ms to get response, and 223 ms overall 17-03-18 00:10:17.848 -07 000000012 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:17.848 -07 000000561 INF: Z Motor: Entering MoveAbsStepped (DAC = 29833, sync = True, waitForSettling = False) 17-03-18 00:10:17.848 -07 000000561 INF: Z Motor: Moving to -0.136567 mm, started at -0.130268 mm, distance = -6.30 um 17-03-18 00:10:17.848 -07 000000482 INF: Y Motor: Last commanded position = 65.7226 mm (105156.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:17.848 -07 000000482 INF: Y Motor: Moving 1341 steps-18 17-03-18 00:10:17.858 -07 000000561 INF: FPGA: Xmit ZDACW 29833\n [Z Motor] 17-03-18 00:10:17.868 -07 000000482 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:17.948 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 1389170736, 0x0, 418435064) end. Duration was 372 ms. 17-03-18 00:10:17.948 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 372 ms. 17-03-18 00:10:17.948 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:17.958 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:17.958 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 532 ms. 17-03-18 00:10:17.968 -07 000000608 INF: FPGA: Recv ZDACW 17-03-18 00:10:17.968 -07 000000561 INF: FPGA: 'ZDACW 29833' command took 0 ms to send, 106 ms to get response, and 121 ms overall 17-03-18 00:10:17.968 -07 000000561 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:17.968 -07 000000608 INF: FPGA: Recv ZDACR 29833 17-03-18 00:10:17.978 -07 000000561 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:10:17.978 -07 000000561 INF: Z Motor: MoveAbsStepped took 125 ms (did NOT wait for settling yet)) 17-03-18 00:10:18.048 -07 000000561 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:18.048 -07 000000012 INF: FPGA: 'FOCMRRFOC' command took 2 ms to send, 197 ms to get response, and 205 ms overall 17-03-18 00:10:18.238 -07 000000608 INF: FPGA: Recv YMVTILE 17-03-18 00:10:18.238 -07 000000482 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 389 ms overall 17-03-18 00:10:18.278 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:18.288 -07 000000561 INF: FPGA: Recv YRDPOS 136272 17-03-18 00:10:18.288 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 2 ms to get response, and 3 ms overall 17-03-18 00:10:18.288 -07 000000482 INF: Y Motor: Encoder = 136272 = motor step 106462.5 = 66.5391 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:18.288 -07 000000482 INF: Y Motor: Position error before settling (table move) = -28 encoder counts (-0.014 mm) 17-03-18 00:10:18.288 -07 000000482 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 389 ms, distance = 0.839 mm, average velocity = 2.156 mm/sec 17-03-18 00:10:18.718 -07 000000438 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:10:19.238 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:19.238 -07 000000561 INF: FPGA: Recv YRDPOS 136272 17-03-18 00:10:19.248 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:19.248 -07 000000482 INF: Y Motor: Encoder = 136272 = motor step 106462.5 = 66.5391 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:19.268 -07 000000482 INF: Y Motor: After move to 66.552605, error = -0.013542, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:19.268 -07 000000482 INF: Y Motor: Position error after settling (table move) = -28 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:19.268 -07 000000482 INF: Y Motor: Moved to 66.5391 after 1645 ms (table move) 17-03-18 00:10:19.278 -07 000000627 INF: Z Motor: EndOfMoveAbsStepped: target = -0.136567 mm (DAC = 29833) 17-03-18 00:10:19.278 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.278 -07 000000438 INF: FPGA: Recv ZSGSRD 30537 17-03-18 00:10:19.288 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.288 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.288 -07 000000482 INF: FPGA: Recv ZSGSRD 30542 17-03-18 00:10:19.298 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.298 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.298 -07 000000438 INF: FPGA: Recv ZSGSRD 30554 17-03-18 00:10:19.308 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.308 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.308 -07 000000012 INF: FPGA: Recv ZSGSRD 30552 17-03-18 00:10:19.318 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.318 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.318 -07 000000438 INF: FPGA: Recv ZSGSRD 30550 17-03-18 00:10:19.328 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.328 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.328 -07 000000561 INF: FPGA: Recv ZSGSRD 30554 17-03-18 00:10:19.338 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.338 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.338 -07 000000438 INF: FPGA: Recv ZSGSRD 30544 17-03-18 00:10:19.348 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.348 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.348 -07 000000012 INF: FPGA: Recv ZSGSRD 30546 17-03-18 00:10:19.358 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.358 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.358 -07 000000438 INF: FPGA: Recv ZSGSRD 30546 17-03-18 00:10:19.368 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.368 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:19.368 -07 000000486 INF: FPGA: Recv ZSGSRD 30544 17-03-18 00:10:19.378 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.378 -07 000000627 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:19.378 -07 000000561 INF: FPGA: Recv ZPIEZORD 26263 17-03-18 00:10:19.388 -07 000000627 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:19.388 -07 000000627 INF: Z Motor: Piezo is near 36.1 V (nominally 0 to 100 V), expect near 45.5 V, since DAC is at 4.552 V. Piezo Error = -9.4 V. 17-03-18 00:10:19.388 -07 000000627 INF: Z Motor: ReadAdcAverage: Commanded Z = -136.6 um, Read 10 ADC samples: Avg = 30547 (-135.8 uncal um), Error = 0.8 uncal um, Median = 30546 (-135.8 uncal um), StdDev = 6 (0.0 uncal um), Range = 17 (0.1 uncal um), Min = 30537 (-135.7 uncal um), Max = 30554 (-135.8 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 10 (0.1 uncal um) 17-03-18 00:10:19.388 -07 000000627 INF: Z Motor: ADC noise = 10, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30547, posNoise = 7, negNoise = 10 17-03-18 00:10:19.388 -07 000000627 INF: Z Motor: After move to -0.136567, error = 0.000003, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:19.388 -07 000000627 INF: Z Motor: Settle wait time = 1426 ms, measure time = 110 ms (allowed 150 ms), total time = 1536 ms 17-03-18 00:10:19.388 -07 000000627 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:19.428 -07 000000627 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1801 ms 17-03-18 00:10:19.428 -07 000000627 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:19.428 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:19.428 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:19.428 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:19.428 -07 000000438 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:19.428 -07 000000482 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:19.428 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:19.428 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:19.428 -07 000000482 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:19.438 -07 000000482 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:19.438 -07 000000482 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:19.448 -07 000000482 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 9 ms. 17-03-18 00:10:19.448 -07 000000482 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 13 ms. 17-03-18 00:10:19.448 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:19.488 -07 000000561 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:19.788 -07 000000627 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:19.788 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:19.788 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:19.798 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) start. 17-03-18 00:10:19.828 -07 000000478 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:19.828 -07 000000482 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:19.828 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 378 ms to get response, and 385 ms overall 17-03-18 00:10:19.918 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 608668944, 0x0, 559484976) end. Duration was 127 ms. 17-03-18 00:10:19.918 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:19.918 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:10:19.918 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 130 ms. 17-03-18 00:10:20.028 -07 000000561 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:20.028 -07 000000012 INF: Autofocus start 17-03-18 00:10:20.028 -07 000000012 INF: MeasureProfile start 17-03-18 00:10:20.028 -07 000000482 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:20.038 -07 000000561 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:20.038 -07 000000561 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:20.038 -07 000000012 INF: MeasureProfile done 17-03-18 00:10:20.038 -07 000000012 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:10:20.038 -07 000000012 INF: Autofocus finished 17-03-18 00:10:20.238 -07 000000486 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:20.238 -07 000000561 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:20.238 -07 000000610 INF: ------------ Scan Snapping tile 9 at Y-position 66.5390625, Z-position -0.136563760305507. ---------- 17-03-18 00:10:20.238 -07 000000610 INF: Cycle 82 Tile 9 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:20.248 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28340, sync = True, waitForSettling = True) 17-03-18 00:10:20.248 -07 000000610 INF: Z Motor: Moving to -0.129732 mm, started at -0.136564 mm, distance = 6.83 um 17-03-18 00:10:20.248 -07 000000610 INF: FPGA: Xmit ZDACW 28340\n [Z Motor] 17-03-18 00:10:20.248 -07 000000608 INF: FPGA: Recv ZDACW 17-03-18 00:10:20.258 -07 000000610 INF: FPGA: 'ZDACW 28340' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.258 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:20.258 -07 000000478 INF: FPGA: Recv ZDACR 28340 17-03-18 00:10:20.268 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.268 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:20.418 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.418 -07 000000486 INF: FPGA: Recv ZSGSRD 29482 17-03-18 00:10:20.428 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:20.428 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.428 -07 000000438 INF: FPGA: Recv ZSGSRD 29490 17-03-18 00:10:20.438 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.438 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.438 -07 000000561 INF: FPGA: Recv ZSGSRD 29488 17-03-18 00:10:20.448 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.448 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.448 -07 000000482 INF: FPGA: Recv ZSGSRD 29482 17-03-18 00:10:20.458 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.458 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.458 -07 000000608 INF: FPGA: Recv ZSGSRD 29483 17-03-18 00:10:20.468 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.468 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.468 -07 000000438 INF: FPGA: Recv ZSGSRD 29491 17-03-18 00:10:20.478 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.478 -07 000000608 INF: FPGA: Recv ZSGSRD 29477 17-03-18 00:10:20.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.488 -07 000000478 INF: FPGA: Recv ZSGSRD 29486 17-03-18 00:10:20.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.498 -07 000000627 INF: FPGA: Recv ZSGSRD 29483 17-03-18 00:10:20.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:20.508 -07 000000012 INF: FPGA: Recv ZSGSRD 29491 17-03-18 00:10:20.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.518 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:20.518 -07 000000561 INF: FPGA: Recv ZPIEZORD 24938 17-03-18 00:10:20.528 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:20.528 -07 000000610 INF: Z Motor: Piezo is near 33.3 V (nominally 0 to 100 V), expect near 43.2 V, since DAC is at 4.324 V. Piezo Error = -10.0 V. 17-03-18 00:10:20.528 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -129.7 um, Read 10 ADC samples: Avg = 29485 (-129.0 uncal um), Error = 0.8 uncal um, Median = 29484 (-129.0 uncal um), StdDev = 5 (0.0 uncal um), Range = 14 (0.1 uncal um), Min = 29477 (-128.9 uncal um), Max = 29491 (-129.0 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 8 (0.1 uncal um) 17-03-18 00:10:20.528 -07 000000610 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29485, posNoise = 6, negNoise = 8 17-03-18 00:10:20.528 -07 000000610 INF: Z Motor: After move to -0.129732, error = -0.000009, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:20.528 -07 000000610 INF: Z Motor: MoveAbsStepped took 281 ms) 17-03-18 00:10:20.528 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:20.528 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:20.528 -07 000000486 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:20.528 -07 000000608 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:20.528 -07 000000012 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:20.528 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:20.528 -07 000000561 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:20.528 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:20.528 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:20.528 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:20.528 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:20.528 -07 000000561 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:20.528 -07 000000012 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:20.528 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:20.538 -07 000000561 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:10:20.538 -07 000000561 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:20.548 -07 000000012 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:10:20.548 -07 000000012 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 19 ms. 17-03-18 00:10:20.548 -07 000000561 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:10:20.548 -07 000000561 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:10:20.548 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28517 150\n [Camera] 17-03-18 00:10:20.748 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:21.788 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:21.788 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:21.788 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:21.788 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:21.788 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:21.788 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:21.798 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:21.798 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2550140976, 0x0, 391868352) start. 17-03-18 00:10:21.798 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:21.798 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 2585563240, 0x0, 418435064) start. 17-03-18 00:10:21.988 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28517 1426 17-03-18 00:10:22.128 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:22.138 -07 000000012 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:22.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:22.198 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2550140976, 0x0, 391868352) end. Duration was 396 ms. 17-03-18 00:10:22.198 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 397 ms. 17-03-18 00:10:22.198 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:22.198 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 2585563240, 0x0, 418435064) end. Duration was 396 ms. 17-03-18 00:10:22.198 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 397 ms. 17-03-18 00:10:22.198 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:22.198 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:22.198 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 410 ms. 17-03-18 00:10:22.198 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:22.208 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 417 ms. 17-03-18 00:10:22.628 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:22.628 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:22.628 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:22.748 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:22.748 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:22.748 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:22.748 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:22.748 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:10:22.748 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:22.748 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:10:22.818 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:22.818 -07 000000382 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:22.818 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28517 150' command took 2 ms to send, 2259 ms to get response, and 2272 ms overall 17-03-18 00:10:22.828 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:22.828 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 10 at Y-position 66.5390625, Z-Position -0.129741247010957. ------ 17-03-18 00:10:22.828 -07 000000448 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:22.828 -07 000000478 INF: Y Motor: MoveYZ optimized starting. Moving Y to 67.3826 mm, moving Z to -0.13647 mm 17-03-18 00:10:22.828 -07 000000478 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:22.828 -07 000000482 INF: Y Motor: Entering MoveAbsStepped (target = 107812 steps, sync = True, waitForSettling = True) 17-03-18 00:10:22.828 -07 000000482 INF: Y Motor: Moving to 67.3826 mm, started at 66.5391 mm, delta = 0.8435 mm 17-03-18 00:10:22.828 -07 000000627 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:22.828 -07 000000438 INF: Z Motor: Entering MoveAbsStepped (DAC = 29812, sync = True, waitForSettling = False) 17-03-18 00:10:22.828 -07 000000438 INF: Z Motor: Moving to -0.136471 mm, started at -0.129741 mm, distance = -6.73 um 17-03-18 00:10:22.838 -07 000000448 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:10:22.838 -07 000000438 INF: FPGA: Xmit ZDACW 29812\n [Z Motor] 17-03-18 00:10:22.838 -07 000000486 INF: FPGA: Recv ZDACW 17-03-18 00:10:22.848 -07 000000438 INF: FPGA: 'ZDACW 29812' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:22.848 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:22.848 -07 000000608 INF: FPGA: Recv YRDPOS 136272 17-03-18 00:10:22.858 -07 000000448 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:22.858 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 20 ms overall 17-03-18 00:10:22.858 -07 000000482 INF: Y Motor: Encoder = 136272 = motor step 106462.5 = 66.5391 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:22.858 -07 000000482 INF: Y Motor: Last commanded position = 66.5526 mm (106484.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:22.858 -07 000000482 INF: Y Motor: Moving 1350 steps-28 17-03-18 00:10:22.868 -07 000000438 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:22.878 -07 000000482 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:23.148 -07 000000012 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 403 ms. 17-03-18 00:10:23.148 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 403 ms. 17-03-18 00:10:23.148 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:10:23.148 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:23.148 -07 000000012 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 403 ms. 17-03-18 00:10:23.148 -07 000000012 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:23.158 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:10:23.158 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 524 ms. 17-03-18 00:10:23.158 -07 000000012 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:10:23.168 -07 000000012 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 416 ms. 17-03-18 00:10:23.168 -07 000000012 INF: FPGA: Recv ZDACR 29812 17-03-18 00:10:23.168 -07 000000012 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:23.168 -07 000000448 INF: FPGA: 'FOCMRRFOC' command took 3 ms to send, 309 ms to get response, and 339 ms overall 17-03-18 00:10:23.178 -07 000000438 INF: FPGA: 'ZDACR' command took 0 ms to send, 299 ms to get response, and 329 ms overall 17-03-18 00:10:23.178 -07 000000438 INF: Z Motor: MoveAbsStepped took 340 ms (did NOT wait for settling yet)) 17-03-18 00:10:23.258 -07 000000448 INF: FPGA: Recv YMVTILE 17-03-18 00:10:23.258 -07 000000482 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 374 ms to get response, and 401 ms overall 17-03-18 00:10:23.298 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:23.308 -07 000000438 INF: FPGA: Recv YRDPOS 137977 17-03-18 00:10:23.308 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:10:23.308 -07 000000482 INF: Y Motor: Encoder = 137977 = motor step 107794.5 = 67.3716 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:23.308 -07 000000482 INF: Y Motor: Position error before settling (table move) = -23 encoder counts (-0.011 mm) 17-03-18 00:10:23.308 -07 000000482 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 401 ms, distance = 0.844 mm, average velocity = 2.104 mm/sec 17-03-18 00:10:24.078 -07 000000448 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.977204, 17-03-18 00:10:24.258 -07 000000482 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:24.258 -07 000000448 INF: FPGA: Recv YRDPOS 137977 17-03-18 00:10:24.268 -07 000000482 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:24.268 -07 000000482 INF: Y Motor: Encoder = 137977 = motor step 107794.5 = 67.3716 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:24.288 -07 000000482 INF: Y Motor: After move to 67.382607, error = -0.011024, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:24.288 -07 000000482 INF: Y Motor: Position error after settling (table move) = -23 encoder counts (-0.011 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:24.288 -07 000000482 INF: Y Motor: Moved to 67.3716 after 1457 ms (table move) 17-03-18 00:10:24.298 -07 000000478 INF: Z Motor: EndOfMoveAbsStepped: target = -0.136471 mm (DAC = 29812) 17-03-18 00:10:24.298 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.298 -07 000000626 INF: FPGA: Recv ZSGSRD 30534 17-03-18 00:10:24.308 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.308 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.308 -07 000000569 INF: FPGA: Recv ZSGSRD 30535 17-03-18 00:10:24.318 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.318 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.318 -07 000000448 INF: FPGA: Recv ZSGSRD 30541 17-03-18 00:10:24.328 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.328 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.328 -07 000000012 INF: FPGA: Recv ZSGSRD 30535 17-03-18 00:10:24.338 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.338 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.338 -07 000000626 INF: FPGA: Recv ZSGSRD 30530 17-03-18 00:10:24.348 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.348 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.348 -07 000000448 INF: FPGA: Recv ZSGSRD 30534 17-03-18 00:10:24.358 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.358 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.358 -07 000000482 INF: FPGA: Recv ZSGSRD 30535 17-03-18 00:10:24.368 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.368 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.368 -07 000000626 INF: FPGA: Recv ZSGSRD 30529 17-03-18 00:10:24.378 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.378 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.388 -07 000000012 INF: FPGA: Recv ZSGSRD 30529 17-03-18 00:10:24.388 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:24.388 -07 000000478 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:24.388 -07 000000448 INF: FPGA: Recv ZSGSRD 30534 17-03-18 00:10:24.398 -07 000000478 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.398 -07 000000478 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:24.398 -07 000000482 INF: FPGA: Recv ZPIEZORD 26308 17-03-18 00:10:24.408 -07 000000478 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:24.408 -07 000000478 INF: Z Motor: Piezo is near 36.2 V (nominally 0 to 100 V), expect near 45.5 V, since DAC is at 4.549 V. Piezo Error = -9.3 V. 17-03-18 00:10:24.408 -07 000000478 INF: Z Motor: ReadAdcAverage: Commanded Z = -136.5 um, Read 10 ADC samples: Avg = 30534 (-135.7 uncal um), Error = 0.8 uncal um, Median = 30534 (-135.7 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 30529 (-135.7 uncal um), Max = 30541 (-135.7 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:10:24.408 -07 000000478 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30534, posNoise = 7, negNoise = 5 17-03-18 00:10:24.408 -07 000000478 INF: Z Motor: After move to -0.136471, error = -0.000010, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:24.408 -07 000000478 INF: Z Motor: Settle wait time = 1460 ms, measure time = 110 ms (allowed 150 ms), total time = 1570 ms 17-03-18 00:10:24.408 -07 000000478 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:24.448 -07 000000478 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1613 ms 17-03-18 00:10:24.448 -07 000000478 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:24.448 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:24.448 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:24.448 -07 000000502 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:24.448 -07 000000448 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:24.448 -07 000000482 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:24.448 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:24.448 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:10:24.448 -07 000000482 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:24.458 -07 000000482 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:24.458 -07 000000482 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:24.458 -07 000000482 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 7 ms. 17-03-18 00:10:24.458 -07 000000482 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 11 ms. 17-03-18 00:10:24.468 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:24.508 -07 000000438 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:24.808 -07 000000626 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:24.808 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:24.808 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:24.808 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1406045800, 0x0, 559484976) start. 17-03-18 00:10:24.848 -07 000000482 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:24.848 -07 000000478 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:24.848 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 376 ms to get response, and 384 ms overall 17-03-18 00:10:24.938 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1406045800, 0x0, 559484976) end. Duration was 127 ms. 17-03-18 00:10:24.938 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:24.938 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:10:24.938 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 129 ms. 17-03-18 00:10:25.048 -07 000000586 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:25.048 -07 000000012 INF: Autofocus start 17-03-18 00:10:25.048 -07 000000012 INF: MeasureProfile start 17-03-18 00:10:25.048 -07 000000502 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:25.058 -07 000000586 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:25.058 -07 000000586 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:25.058 -07 000000012 INF: MeasureProfile done 17-03-18 00:10:25.058 -07 000000012 INF: X=16 Z correction [um]=0.0530179 17-03-18 00:10:25.058 -07 000000012 INF: Autofocus finished 17-03-18 00:10:25.258 -07 000000448 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:25.258 -07 000000586 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:25.258 -07 000000610 INF: ------------ Scan Snapping tile 10 at Y-position 67.37158203125, Z-position -0.136480245547664. ---------- 17-03-18 00:10:25.258 -07 000000610 INF: Cycle 82 Tile 10 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:25.268 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28336, sync = True, waitForSettling = True) 17-03-18 00:10:25.268 -07 000000610 INF: Z Motor: Moving to -0.129714 mm, started at -0.136480 mm, distance = 6.77 um 17-03-18 00:10:25.268 -07 000000610 INF: FPGA: Xmit ZDACW 28336\n [Z Motor] 17-03-18 00:10:25.268 -07 000000502 INF: FPGA: Recv ZDACW 17-03-18 00:10:25.278 -07 000000610 INF: FPGA: 'ZDACW 28336' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:25.278 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:25.288 -07 000000448 INF: FPGA: Recv ZDACR 28336 17-03-18 00:10:25.288 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:25.288 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:25.438 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.438 -07 000000586 INF: FPGA: Recv ZSGSRD 29481 17-03-18 00:10:25.448 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:25.448 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.448 -07 000000012 INF: FPGA: Recv ZSGSRD 29483 17-03-18 00:10:25.458 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.458 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.458 -07 000000478 INF: FPGA: Recv ZSGSRD 29482 17-03-18 00:10:25.468 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.468 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.468 -07 000000012 INF: FPGA: Recv ZSGSRD 29482 17-03-18 00:10:25.478 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.488 -07 000000502 INF: FPGA: Recv ZSGSRD 29481 17-03-18 00:10:25.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:25.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.488 -07 000000438 INF: FPGA: Recv ZSGSRD 29489 17-03-18 00:10:25.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.498 -07 000000586 INF: FPGA: Recv ZSGSRD 29486 17-03-18 00:10:25.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.508 -07 000000438 INF: FPGA: Recv ZSGSRD 29479 17-03-18 00:10:25.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.518 -07 000000586 INF: FPGA: Recv ZSGSRD 29484 17-03-18 00:10:25.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:25.528 -07 000000438 INF: FPGA: Recv ZSGSRD 29476 17-03-18 00:10:25.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.538 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:25.538 -07 000000626 INF: FPGA: Recv ZPIEZORD 24890 17-03-18 00:10:25.548 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:25.548 -07 000000610 INF: Z Motor: Piezo is near 33.2 V (nominally 0 to 100 V), expect near 43.2 V, since DAC is at 4.324 V. Piezo Error = -10.1 V. 17-03-18 00:10:25.548 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -129.7 um, Read 10 ADC samples: Avg = 29482 (-128.9 uncal um), Error = 0.8 uncal um, Median = 29482 (-128.9 uncal um), StdDev = 4 (0.0 uncal um), Range = 13 (0.1 uncal um), Min = 29476 (-128.9 uncal um), Max = 29489 (-129.0 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:10:25.548 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29482, posNoise = 7, negNoise = 6 17-03-18 00:10:25.548 -07 000000610 INF: Z Motor: After move to -0.129714, error = -0.000008, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:25.548 -07 000000610 INF: Z Motor: MoveAbsStepped took 280 ms) 17-03-18 00:10:25.548 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:25.548 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:25.548 -07 000000448 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:25.548 -07 000000482 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:25.548 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:25.548 -07 000000569 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:25.548 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:25.548 -07 000000438 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:25.548 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:25.548 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:25.548 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:25.548 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:25.558 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:25.558 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:25.558 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:10:25.558 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:25.568 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:10:25.568 -07 000000482 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:25.568 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 16 ms. 17-03-18 00:10:25.568 -07 000000569 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:25.568 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28513 150\n [Camera] 17-03-18 00:10:25.788 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP turning red on 199 17-03-18 00:10:26.818 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:26.818 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:26.818 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:26.818 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:26.818 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) start. 17-03-18 00:10:26.818 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:26.818 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:26.818 -07 000000586 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:26.818 -07 000000586 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:26.818 -07 000000586 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) start. 17-03-18 00:10:27.008 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28513 1422 17-03-18 00:10:27.138 -07 000000438 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:27.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:27.158 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:27.208 -07 000000586 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) end. Duration was 386 ms. 17-03-18 00:10:27.208 -07 000000586 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 387 ms. 17-03-18 00:10:27.208 -07 000000586 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:27.208 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1476890328, 0x0, 391868352) end. Duration was 392 ms. 17-03-18 00:10:27.208 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 392 ms. 17-03-18 00:10:27.208 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:27.208 -07 000000586 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:27.218 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:27.218 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 396 ms. 17-03-18 00:10:27.218 -07 000000586 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 397 ms. 17-03-18 00:10:27.658 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:27.658 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:27.658 -07 000000502 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:27.658 -07 000000502 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:27.658 -07 000000502 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) start. 17-03-18 00:10:27.658 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:27.658 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:27.658 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:27.658 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:27.658 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) start. 17-03-18 00:10:27.848 -07 000000478 INF: FPGA: Recv @CAMDBLSNAP 2264 17-03-18 00:10:27.848 -07 000000448 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:27.848 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28513 150' command took 2 ms to send, 2266 ms to get response, and 2277 ms overall 17-03-18 00:10:27.848 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:27.848 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 11 at Y-position 67.37158203125, Z-Position -0.129721974374532. ------ 17-03-18 00:10:27.858 -07 000000501 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:27.858 -07 000000586 INF: Y Motor: MoveYZ optimized starting. Moving Y to 68.2126 mm, moving Z to -0.13620 mm 17-03-18 00:10:27.858 -07 000000586 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:27.858 -07 000000448 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:27.858 -07 000000448 INF: Y Motor: Entering MoveAbsStepped (target = 109140 steps, sync = True, waitForSettling = True) 17-03-18 00:10:27.858 -07 000000448 INF: Y Motor: Moving to 68.2126 mm, started at 67.3716 mm, delta = 0.8410 mm 17-03-18 00:10:27.858 -07 000000482 INF: Z Motor: Entering MoveAbsStepped (DAC = 29753, sync = True, waitForSettling = False) 17-03-18 00:10:27.858 -07 000000482 INF: Z Motor: Moving to -0.136201 mm, started at -0.129722 mm, distance = -6.48 um 17-03-18 00:10:27.868 -07 000000501 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:27.868 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:27.868 -07 000000512 INF: FPGA: Recv YRDPOS 137978 17-03-18 00:10:27.878 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 12 ms overall 17-03-18 00:10:27.878 -07 000000501 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:27.878 -07 000000448 INF: Y Motor: Encoder = 137978 = motor step 107795.3 = 67.3721 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:27.878 -07 000000448 INF: Y Motor: Last commanded position = 67.3826 mm (107812.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:27.878 -07 000000448 INF: Y Motor: Moving 1345 steps-23 17-03-18 00:10:27.888 -07 000000482 INF: FPGA: Xmit ZDACW 29753\n [Z Motor] 17-03-18 00:10:27.888 -07 000000626 INF: FPGA: Recv ZDACW 17-03-18 00:10:27.898 -07 000000448 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:27.898 -07 000000482 INF: FPGA: 'ZDACW 29753' command took 0 ms to send, 0 ms to get response, and 30 ms overall 17-03-18 00:10:27.908 -07 000000482 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:27.908 -07 000000626 INF: FPGA: Recv ZDACR 29753 17-03-18 00:10:27.918 -07 000000482 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:10:27.918 -07 000000482 INF: Z Motor: MoveAbsStepped took 50 ms (did NOT wait for settling yet)) 17-03-18 00:10:28.058 -07 000000502 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) end. Duration was 402 ms. 17-03-18 00:10:28.058 -07 000000502 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:10:28.058 -07 000000502 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:28.058 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:10:28.058 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 400 ms. 17-03-18 00:10:28.058 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:28.068 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:28.068 -07 000000502 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:10:28.068 -07 000000502 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 410 ms. 17-03-18 00:10:28.078 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:10:28.078 -07 000000012 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:28.078 -07 000000501 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 193 ms to get response, and 212 ms overall 17-03-18 00:10:28.268 -07 000000482 INF: FPGA: Recv YMVTILE 17-03-18 00:10:28.268 -07 000000448 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 393 ms overall 17-03-18 00:10:28.328 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:28.328 -07 000000512 INF: FPGA: Recv YRDPOS 139684 17-03-18 00:10:28.338 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:28.338 -07 000000448 INF: Y Motor: Encoder = 139684 = motor step 109128.1 = 68.2051 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:28.338 -07 000000448 INF: Y Motor: Position error before settling (table move) = -15 encoder counts (-0.007 mm) 17-03-18 00:10:28.338 -07 000000448 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 394 ms, distance = 0.841 mm, average velocity = 2.135 mm/sec 17-03-18 00:10:29.288 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:29.288 -07 000000512 INF: FPGA: Recv YRDPOS 139684 17-03-18 00:10:29.298 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:29.298 -07 000000448 INF: Y Motor: Encoder = 139684 = motor step 109128.1 = 68.2051 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:29.318 -07 000000448 INF: Y Motor: After move to 68.212608, error = -0.007530, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:29.318 -07 000000448 INF: Y Motor: Position error after settling (table move) = -15 encoder counts (-0.007 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:29.318 -07 000000448 INF: Y Motor: Moved to 68.2051 after 1459 ms (table move) 17-03-18 00:10:29.328 -07 000000586 INF: Z Motor: EndOfMoveAbsStepped: target = -0.136201 mm (DAC = 29753) 17-03-18 00:10:29.328 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.328 -07 000000438 INF: FPGA: Recv ZSGSRD 30500 17-03-18 00:10:29.338 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.338 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.338 -07 000000512 INF: FPGA: Recv ZSGSRD 30496 17-03-18 00:10:29.348 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.348 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.348 -07 000000448 INF: FPGA: Recv ZSGSRD 30487 17-03-18 00:10:29.358 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.358 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.358 -07 000000448 INF: FPGA: Recv ZSGSRD 30495 17-03-18 00:10:29.368 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.368 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.368 -07 000000512 INF: FPGA: Recv ZSGSRD 30482 17-03-18 00:10:29.378 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.378 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.378 -07 000000438 INF: FPGA: Recv ZSGSRD 30489 17-03-18 00:10:29.388 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.388 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.388 -07 000000512 INF: FPGA: Recv ZSGSRD 30492 17-03-18 00:10:29.398 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.398 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.398 -07 000000438 INF: FPGA: Recv ZSGSRD 30492 17-03-18 00:10:29.408 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.408 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.408 -07 000000448 INF: FPGA: Recv ZSGSRD 30494 17-03-18 00:10:29.418 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.418 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:29.418 -07 000000438 INF: FPGA: Recv ZSGSRD 30490 17-03-18 00:10:29.428 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.428 -07 000000586 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:29.428 -07 000000512 INF: FPGA: Recv ZPIEZORD 26244 17-03-18 00:10:29.438 -07 000000586 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:29.438 -07 000000586 INF: Z Motor: Piezo is near 36.1 V (nominally 0 to 100 V), expect near 45.4 V, since DAC is at 4.540 V. Piezo Error = -9.3 V. 17-03-18 00:10:29.438 -07 000000586 INF: Z Motor: ReadAdcAverage: Commanded Z = -136.2 um, Read 10 ADC samples: Avg = 30492 (-135.4 uncal um), Error = 0.8 uncal um, Median = 30492 (-135.4 uncal um), StdDev = 5 (0.0 uncal um), Range = 18 (0.1 uncal um), Min = 30482 (-135.4 uncal um), Max = 30500 (-135.5 uncal um), Pos Noise = 8 (0.1 uncal um), Neg Noise = 10 (0.1 uncal um) 17-03-18 00:10:29.438 -07 000000586 INF: Z Motor: ADC noise = 10, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30492, posNoise = 8, negNoise = 10 17-03-18 00:10:29.438 -07 000000586 INF: Z Motor: After move to -0.136201, error = -0.000010, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:29.438 -07 000000586 INF: Z Motor: Settle wait time = 1460 ms, measure time = 111 ms (allowed 150 ms), total time = 1571 ms 17-03-18 00:10:29.438 -07 000000586 INF: Z Motor: Waiting extra 39 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:29.448 -07 000000438 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:10:29.478 -07 000000586 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1616 ms 17-03-18 00:10:29.478 -07 000000586 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:29.478 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:29.478 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:29.478 -07 000000476 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:29.478 -07 000000569 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:29.478 -07 000000627 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:29.478 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:29.478 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:10:29.478 -07 000000627 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:29.488 -07 000000627 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:29.488 -07 000000627 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:29.488 -07 000000627 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 6 ms. 17-03-18 00:10:29.488 -07 000000627 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 10 ms. 17-03-18 00:10:29.498 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:29.538 -07 000000608 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:29.838 -07 000000448 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:29.838 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:29.838 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:29.958 -07 000000424 INF: FPGA: Recv @CAMSNAP 385 17-03-18 00:10:29.958 -07 000000424 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:29.958 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 453 ms to get response, and 460 ms overall 17-03-18 00:10:29.958 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1512312592, 0x0, 559484976) start. 17-03-18 00:10:30.068 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1512312592, 0x0, 559484976) end. Duration was 115 ms. 17-03-18 00:10:30.068 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:30.068 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:10:30.068 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 232 ms. 17-03-18 00:10:30.158 -07 000000569 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:30.158 -07 000000448 INF: Autofocus start 17-03-18 00:10:30.158 -07 000000448 INF: MeasureProfile start 17-03-18 00:10:30.158 -07 000000608 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:30.168 -07 000000569 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:10:30.168 -07 000000569 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:30.168 -07 000000448 INF: MeasureProfile done 17-03-18 00:10:30.168 -07 000000448 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:10:30.168 -07 000000448 INF: Autofocus finished 17-03-18 00:10:30.368 -07 000000608 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:30.368 -07 000000569 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:30.368 -07 000000610 INF: ------------ Scan Snapping tile 11 at Y-position 68.205078125, Z-position -0.13621042863771. ---------- 17-03-18 00:10:30.368 -07 000000610 INF: Cycle 82 Tile 11 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:30.378 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28260, sync = True, waitForSettling = True) 17-03-18 00:10:30.378 -07 000000610 INF: Z Motor: Moving to -0.129366 mm, started at -0.136210 mm, distance = 6.84 um 17-03-18 00:10:30.378 -07 000000610 INF: FPGA: Xmit ZDACW 28260\n [Z Motor] 17-03-18 00:10:30.388 -07 000000448 INF: FPGA: Recv ZDACW 17-03-18 00:10:30.388 -07 000000610 INF: FPGA: 'ZDACW 28260' command took 0 ms to send, 1 ms to get response, and 10 ms overall 17-03-18 00:10:30.388 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:30.388 -07 000000448 INF: FPGA: Recv ZDACR 28260 17-03-18 00:10:30.398 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:30.398 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:30.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.548 -07 000000448 INF: FPGA: Recv ZSGSRD 29428 17-03-18 00:10:30.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:30.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.558 -07 000000476 INF: FPGA: Recv ZSGSRD 29424 17-03-18 00:10:30.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.568 -07 000000586 INF: FPGA: Recv ZSGSRD 29430 17-03-18 00:10:30.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.578 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.578 -07 000000586 INF: FPGA: Recv ZSGSRD 29431 17-03-18 00:10:30.588 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.588 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.588 -07 000000586 INF: FPGA: Recv ZSGSRD 29426 17-03-18 00:10:30.598 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.598 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.598 -07 000000627 INF: FPGA: Recv ZSGSRD 29430 17-03-18 00:10:30.608 -07 000000610 INF: FPGA: 'ZSGSRD' command took 1 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.608 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.608 -07 000000569 INF: FPGA: Recv ZSGSRD 29428 17-03-18 00:10:30.618 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.618 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.618 -07 000000608 INF: FPGA: Recv ZSGSRD 29432 17-03-18 00:10:30.628 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.628 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.628 -07 000000586 INF: FPGA: Recv ZSGSRD 29425 17-03-18 00:10:30.638 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.638 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:30.638 -07 000000438 INF: FPGA: Recv ZSGSRD 29426 17-03-18 00:10:30.648 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.648 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:30.648 -07 000000438 INF: FPGA: Recv ZPIEZORD 24812 17-03-18 00:10:30.658 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:30.658 -07 000000610 INF: Z Motor: Piezo is near 33.0 V (nominally 0 to 100 V), expect near 43.1 V, since DAC is at 4.312 V. Piezo Error = -10.1 V. 17-03-18 00:10:30.658 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -129.4 um, Read 10 ADC samples: Avg = 29428 (-128.6 uncal um), Error = 0.8 uncal um, Median = 29428 (-128.6 uncal um), StdDev = 3 (0.0 uncal um), Range = 8 (0.1 uncal um), Min = 29424 (-128.6 uncal um), Max = 29432 (-128.6 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:10:30.658 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29428, posNoise = 4, negNoise = 4 17-03-18 00:10:30.658 -07 000000610 INF: Z Motor: After move to -0.129366, error = -0.000009, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:30.658 -07 000000610 INF: Z Motor: MoveAbsStepped took 280 ms) 17-03-18 00:10:30.658 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:30.658 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:30.658 -07 000000486 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:30.658 -07 000000512 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:30.658 -07 000000448 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:30.658 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:30.658 -07 000000569 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:30.658 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:30.658 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:30.658 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:30.658 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:30.658 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:30.668 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:30.668 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:30.668 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:10:30.668 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:30.678 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:10:30.678 -07 000000448 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:30.678 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:10:30.678 -07 000000569 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:30.688 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28437 150\n [Camera] 17-03-18 00:10:30.888 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP turning red on 195 17-03-18 00:10:31.918 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:31.918 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:31.918 -07 000000424 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:31.928 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:31.928 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:31.928 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:31.928 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:31.928 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 1389170736, 0x0, 418435064) start. 17-03-18 00:10:31.928 -07 000000424 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:31.928 -07 000000424 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:10:32.118 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28437 1422 17-03-18 00:10:32.138 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:32.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:32.268 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:32.318 -07 000000448 INF: Camera: sBCa_ReadImage(785074704, 1389170736, 0x0, 418435064) end. Duration was 391 ms. 17-03-18 00:10:32.318 -07 000000448 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 391 ms. 17-03-18 00:10:32.318 -07 000000448 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:32.328 -07 000000448 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:32.328 -07 000000424 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 395 ms. 17-03-18 00:10:32.328 -07 000000424 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 395 ms. 17-03-18 00:10:32.328 -07 000000424 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:32.328 -07 000000424 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:32.328 -07 000000424 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 407 ms. 17-03-18 00:10:32.328 -07 000000448 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 406 ms. 17-03-18 00:10:32.768 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:32.768 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:32.768 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:32.768 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:32.768 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:32.768 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:32.888 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:32.888 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) start. 17-03-18 00:10:32.888 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:32.888 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) start. 17-03-18 00:10:32.958 -07 000000608 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:32.958 -07 000000569 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:32.958 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28437 150' command took 2 ms to send, 2262 ms to get response, and 2272 ms overall 17-03-18 00:10:32.958 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:32.958 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 12 at Y-position 68.205078125, Z-Position -0.129375066918876. ------ 17-03-18 00:10:32.958 -07 000000586 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:32.958 -07 000000627 INF: Y Motor: MoveYZ optimized starting. Moving Y to 69.0426 mm, moving Z to -0.13603 mm 17-03-18 00:10:32.958 -07 000000627 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:32.968 -07 000000569 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:32.968 -07 000000586 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 2 ms to get response, and 3 ms overall 17-03-18 00:10:32.968 -07 000000476 INF: Z Motor: Entering MoveAbsStepped (DAC = 29715, sync = True, waitForSettling = False) 17-03-18 00:10:32.968 -07 000000476 INF: Z Motor: Moving to -0.136027 mm, started at -0.129375 mm, distance = -6.65 um 17-03-18 00:10:32.968 -07 000000476 INF: FPGA: Xmit ZDACW 29715\n [Z Motor] 17-03-18 00:10:32.968 -07 000000382 INF: Y Motor: Entering MoveAbsStepped (target = 110468 steps, sync = True, waitForSettling = True) 17-03-18 00:10:32.968 -07 000000382 INF: Y Motor: Moving to 69.0426 mm, started at 68.2051 mm, delta = 0.8375 mm 17-03-18 00:10:32.968 -07 000000448 INF: FPGA: Recv ZDACW 17-03-18 00:10:32.978 -07 000000586 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:32.978 -07 000000476 INF: FPGA: 'ZDACW 29715' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:32.988 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:32.988 -07 000000448 INF: FPGA: Recv YRDPOS 139684 17-03-18 00:10:32.998 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 26 ms overall 17-03-18 00:10:32.998 -07 000000382 INF: Y Motor: Encoder = 139684 = motor step 109128.1 = 68.2051 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:32.998 -07 000000382 INF: Y Motor: Last commanded position = 68.2126 mm (109140.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:32.998 -07 000000382 INF: Y Motor: Moving 1340 steps-15 17-03-18 00:10:32.998 -07 000000476 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:32.998 -07 000000512 INF: FPGA: Recv ZDACR 29715 17-03-18 00:10:33.008 -07 000000476 INF: FPGA: 'ZDACR' command took 1 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:10:33.008 -07 000000476 INF: Z Motor: MoveAbsStepped took 37 ms (did NOT wait for settling yet)) 17-03-18 00:10:33.008 -07 000000382 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:33.178 -07 000000569 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:33.178 -07 000000586 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 192 ms to get response, and 210 ms overall 17-03-18 00:10:33.278 -07 000000424 INF: Camera: sBCa_ReadImage(785074704, 1476890328, 0x0, 418435064) end. Duration was 396 ms. 17-03-18 00:10:33.278 -07 000000424 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 396 ms. 17-03-18 00:10:33.278 -07 000000424 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:33.288 -07 000000486 INF: Camera: sBCa_ReadImage(785074288, 1441468064, 0x0, 391868352) end. Duration was 395 ms. 17-03-18 00:10:33.288 -07 000000486 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 396 ms. 17-03-18 00:10:33.288 -07 000000486 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:33.288 -07 000000424 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:33.288 -07 000000486 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:33.288 -07 000000486 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 523 ms. 17-03-18 00:10:33.288 -07 000000424 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 525 ms. 17-03-18 00:10:33.388 -07 000000561 INF: FPGA: Recv YMVTILE 17-03-18 00:10:33.388 -07 000000382 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 371 ms to get response, and 391 ms overall 17-03-18 00:10:33.428 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:33.428 -07 000000608 INF: FPGA: Recv YRDPOS 141367 17-03-18 00:10:33.438 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:33.438 -07 000000382 INF: Y Motor: Encoder = 141367 = motor step 110443.0 = 69.0269 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:33.438 -07 000000382 INF: Y Motor: Position error before settling (table move) = -32 encoder counts (-0.016 mm) 17-03-18 00:10:33.438 -07 000000382 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 391 ms, distance = 0.838 mm, average velocity = 2.142 mm/sec 17-03-18 00:10:34.388 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:34.388 -07 000000512 INF: FPGA: Recv YRDPOS 141368 17-03-18 00:10:34.398 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:34.398 -07 000000382 INF: Y Motor: Encoder = 141368 = motor step 110443.8 = 69.0273 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:34.418 -07 000000382 INF: Y Motor: After move to 69.042610, error = -0.015266, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:34.418 -07 000000382 INF: Y Motor: Position error after settling (table move) = -31 encoder counts (-0.015 mm), Delta during settling = 1 counts (0.000 mm) 17-03-18 00:10:34.418 -07 000000382 INF: Y Motor: Moved to 69.0273 after 1454 ms (table move) 17-03-18 00:10:34.428 -07 000000627 INF: Z Motor: EndOfMoveAbsStepped: target = -0.136027 mm (DAC = 29715) 17-03-18 00:10:34.428 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.428 -07 000000486 INF: FPGA: Recv ZSGSRD 30465 17-03-18 00:10:34.438 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:34.438 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.438 -07 000000424 INF: FPGA: Recv ZSGSRD 30468 17-03-18 00:10:34.448 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:34.448 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.448 -07 000000502 INF: FPGA: Recv ZSGSRD 30470 17-03-18 00:10:34.458 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:34.458 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.458 -07 000000424 INF: FPGA: Recv ZSGSRD 30462 17-03-18 00:10:34.468 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:34.468 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.468 -07 000000486 INF: FPGA: Recv ZSGSRD 30460 17-03-18 00:10:34.478 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:34.478 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.478 -07 000000502 INF: FPGA: Recv ZSGSRD 30462 17-03-18 00:10:34.488 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:34.498 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.498 -07 000000486 INF: FPGA: Recv ZSGSRD 30463 17-03-18 00:10:34.508 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:10:34.528 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.528 -07 000000502 INF: FPGA: Recv ZSGSRD 30468 17-03-18 00:10:34.538 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:10:34.538 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.538 -07 000000502 INF: FPGA: Recv ZSGSRD 30465 17-03-18 00:10:34.548 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:34.558 -07 000000627 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:34.558 -07 000000512 INF: FPGA: Recv ZSGSRD 30463 17-03-18 00:10:34.568 -07 000000627 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:10:34.568 -07 000000627 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:34.568 -07 000000502 INF: FPGA: Recv ZPIEZORD 26259 17-03-18 00:10:34.578 -07 000000627 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:34.578 -07 000000627 INF: Z Motor: Piezo is near 36.1 V (nominally 0 to 100 V), expect near 45.3 V, since DAC is at 4.534 V. Piezo Error = -9.2 V. 17-03-18 00:10:34.578 -07 000000627 INF: Z Motor: ReadAdcAverage: Commanded Z = -136.0 um, Read 10 ADC samples: Avg = 30465 (-135.2 uncal um), Error = 0.8 uncal um, Median = 30464 (-135.2 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 30460 (-135.2 uncal um), Max = 30470 (-135.3 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:10:34.578 -07 000000627 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30465, posNoise = 5, negNoise = 5 17-03-18 00:10:34.578 -07 000000627 INF: Z Motor: After move to -0.136027, error = -0.000010, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:34.578 -07 000000627 INF: Z Motor: Settle wait time = 1459 ms, measure time = 148 ms (allowed 150 ms), total time = 1607 ms 17-03-18 00:10:34.578 -07 000000627 INF: Z Motor: Waiting extra 2 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:34.588 -07 000000627 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1620 ms 17-03-18 00:10:34.588 -07 000000627 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:34.588 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:34.588 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:34.588 -07 000000486 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:34.588 -07 000000512 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:34.588 -07 000000476 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:34.588 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:34.588 -07 000000476 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:34.588 -07 000000476 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:34.598 -07 000000476 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:34.598 -07 000000476 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:34.598 -07 000000476 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 6 ms. 17-03-18 00:10:34.598 -07 000000476 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 10 ms. 17-03-18 00:10:34.608 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:34.648 -07 000000476 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:34.828 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:10:34.948 -07 000000476 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:34.948 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:34.948 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:34.948 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1389170736, 0x0, 559484976) start. 17-03-18 00:10:34.988 -07 000000627 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:34.988 -07 000000502 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:34.988 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 376 ms to get response, and 385 ms overall 17-03-18 00:10:35.078 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1389170736, 0x0, 559484976) end. Duration was 124 ms. 17-03-18 00:10:35.078 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:35.078 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:10:35.078 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 127 ms. 17-03-18 00:10:35.088 -07 000000627 INF: Autofocus start 17-03-18 00:10:35.088 -07 000000627 INF: MeasureProfile start 17-03-18 00:10:35.088 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:35.088 -07 000000486 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:35.098 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:10:35.098 -07 000000512 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:35.098 -07 000000627 INF: MeasureProfile done 17-03-18 00:10:35.098 -07 000000627 INF: X=-16 Z correction [um]=-0.08128808 17-03-18 00:10:35.098 -07 000000627 INF: Autofocus finished 17-03-18 00:10:35.298 -07 000000627 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:35.298 -07 000000512 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:35.298 -07 000000610 INF: ------------ Scan Snapping tile 12 at Y-position 69.02734375, Z-position -0.136036974909883. ---------- 17-03-18 00:10:35.298 -07 000000610 INF: Cycle 82 Tile 12 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:35.308 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28210, sync = True, waitForSettling = True) 17-03-18 00:10:35.308 -07 000000610 INF: Z Motor: Moving to -0.129137 mm, started at -0.136037 mm, distance = 6.90 um 17-03-18 00:10:35.308 -07 000000610 INF: FPGA: Xmit ZDACW 28210\n [Z Motor] 17-03-18 00:10:35.308 -07 000000512 INF: FPGA: Recv ZDACW 17-03-18 00:10:35.318 -07 000000610 INF: FPGA: 'ZDACW 28210' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:35.318 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:35.318 -07 000000502 INF: FPGA: Recv ZDACR 28210 17-03-18 00:10:35.328 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.328 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:35.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.478 -07 000000627 INF: FPGA: Recv ZSGSRD 29389 17-03-18 00:10:35.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:35.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.488 -07 000000476 INF: FPGA: Recv ZSGSRD 29394 17-03-18 00:10:35.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.498 -07 000000512 INF: FPGA: Recv ZSGSRD 29393 17-03-18 00:10:35.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.508 -07 000000626 INF: FPGA: Recv ZSGSRD 29393 17-03-18 00:10:35.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.518 -07 000000627 INF: FPGA: Recv ZSGSRD 29394 17-03-18 00:10:35.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.528 -07 000000626 INF: FPGA: Recv ZSGSRD 29389 17-03-18 00:10:35.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.538 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.538 -07 000000486 INF: FPGA: Recv ZSGSRD 29394 17-03-18 00:10:35.548 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.548 -07 000000476 INF: FPGA: Recv ZSGSRD 29394 17-03-18 00:10:35.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.558 -07 000000486 INF: FPGA: Recv ZSGSRD 29388 17-03-18 00:10:35.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:35.568 -07 000000476 INF: FPGA: Recv ZSGSRD 29391 17-03-18 00:10:35.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.578 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:35.578 -07 000000512 INF: FPGA: Recv ZPIEZORD 24780 17-03-18 00:10:35.588 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:35.588 -07 000000610 INF: Z Motor: Piezo is near 32.9 V (nominally 0 to 100 V), expect near 43.0 V, since DAC is at 4.305 V. Piezo Error = -10.1 V. 17-03-18 00:10:35.588 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -129.1 um, Read 10 ADC samples: Avg = 29392 (-128.4 uncal um), Error = 0.8 uncal um, Median = 29393 (-128.4 uncal um), StdDev = 2 (0.0 uncal um), Range = 6 (0.0 uncal um), Min = 29388 (-128.3 uncal um), Max = 29394 (-128.4 uncal um), Pos Noise = 2 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:10:35.588 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29392, posNoise = 2, negNoise = 4 17-03-18 00:10:35.588 -07 000000610 INF: Z Motor: After move to -0.129137, error = -0.000007, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:35.588 -07 000000610 INF: Z Motor: MoveAbsStepped took 279 ms) 17-03-18 00:10:35.588 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:35.588 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:35.588 -07 000000476 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:35.588 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:35.588 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:35.588 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:35.588 -07 000000626 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:35.588 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:35.588 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:35.588 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:35.588 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:35.588 -07 000000626 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:35.598 -07 000000626 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:10:35.598 -07 000000626 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:35.598 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 4 ms. 17-03-18 00:10:35.598 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:35.608 -07 000000626 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 16 ms. 17-03-18 00:10:35.608 -07 000000626 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:35.608 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:10:35.608 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:35.618 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28387 150\n [Camera] 17-03-18 00:10:35.818 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:36.848 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:36.848 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:36.848 -07 000000012 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:36.848 -07 000000012 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:36.848 -07 000000012 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:10:36.858 -07 000000476 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:36.858 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:36.858 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:36.858 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:36.858 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) start. 17-03-18 00:10:37.048 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28387 1422 17-03-18 00:10:37.138 -07 000000626 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:37.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:37.198 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:37.248 -07 000000012 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 398 ms. 17-03-18 00:10:37.248 -07 000000012 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 398 ms. 17-03-18 00:10:37.248 -07 000000012 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:37.258 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 1077874736, 0x0, 418435064) end. Duration was 398 ms. 17-03-18 00:10:37.258 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:10:37.258 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:37.258 -07 000000012 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:37.258 -07 000000012 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:10:37.258 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:37.268 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 409 ms. 17-03-18 00:10:37.698 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:37.698 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:37.698 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:37.698 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:37.698 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) start. 17-03-18 00:10:37.698 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:37.698 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:37.698 -07 000000501 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:37.698 -07 000000501 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:37.698 -07 000000501 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) start. 17-03-18 00:10:37.888 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP 2267 17-03-18 00:10:37.888 -07 000000476 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:37.888 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28387 150' command took 2 ms to send, 2265 ms to get response, and 2274 ms overall 17-03-18 00:10:37.888 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:37.888 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 13 at Y-position 69.02734375, Z-Position -0.129143795281773. ------ 17-03-18 00:10:37.898 -07 000000482 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:37.898 -07 000000626 INF: Y Motor: MoveYZ optimized starting. Moving Y to 69.8726 mm, moving Z to -0.13543 mm 17-03-18 00:10:37.898 -07 000000626 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:37.898 -07 000000478 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:37.898 -07 000000547 INF: Y Motor: Entering MoveAbsStepped (target = 111796 steps, sync = True, waitForSettling = True) 17-03-18 00:10:37.898 -07 000000547 INF: Y Motor: Moving to 69.8726 mm, started at 69.0273 mm, delta = 0.8453 mm 17-03-18 00:10:37.898 -07 000000512 INF: Z Motor: Entering MoveAbsStepped (DAC = 29584, sync = True, waitForSettling = False) 17-03-18 00:10:37.898 -07 000000512 INF: Z Motor: Moving to -0.135427 mm, started at -0.129144 mm, distance = -6.28 um 17-03-18 00:10:37.908 -07 000000482 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:37.908 -07 000000512 INF: FPGA: Xmit ZDACW 29584\n [Z Motor] 17-03-18 00:10:37.908 -07 000000486 INF: FPGA: Recv ZDACW 17-03-18 00:10:37.918 -07 000000512 INF: FPGA: 'ZDACW 29584' command took 0 ms to send, 0 ms to get response, and 14 ms overall 17-03-18 00:10:37.918 -07 000000547 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:37.918 -07 000000448 INF: FPGA: Recv YRDPOS 141368 17-03-18 00:10:37.928 -07 000000482 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:37.928 -07 000000547 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 24 ms overall 17-03-18 00:10:37.928 -07 000000547 INF: Y Motor: Encoder = 141368 = motor step 110443.8 = 69.0273 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:37.928 -07 000000547 INF: Y Motor: Last commanded position = 69.0426 mm (110468.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:37.928 -07 000000547 INF: Y Motor: Moving 1352 steps-32 17-03-18 00:10:37.938 -07 000000512 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:37.938 -07 000000561 INF: FPGA: Recv ZDACR 29584 17-03-18 00:10:37.948 -07 000000547 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:37.948 -07 000000512 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:10:37.948 -07 000000512 INF: Z Motor: MoveAbsStepped took 44 ms (did NOT wait for settling yet)) 17-03-18 00:10:38.088 -07 000000501 INF: Camera: sBCa_ReadImage(785074704, 1148719264, 0x0, 418435064) end. Duration was 394 ms. 17-03-18 00:10:38.088 -07 000000501 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 394 ms. 17-03-18 00:10:38.088 -07 000000501 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:38.098 -07 000000627 INF: Camera: sBCa_ReadImage(785074288, 1113297000, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:10:38.098 -07 000000627 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 399 ms. 17-03-18 00:10:38.098 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:38.098 -07 000000501 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:38.098 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 6 ms. 17-03-18 00:10:38.098 -07 000000627 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 406 ms. 17-03-18 00:10:38.098 -07 000000501 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 405 ms. 17-03-18 00:10:38.128 -07 000000438 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:38.128 -07 000000482 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 192 ms to get response, and 222 ms overall 17-03-18 00:10:38.318 -07 000000502 INF: FPGA: Recv YMVTILE 17-03-18 00:10:38.318 -07 000000547 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 397 ms overall 17-03-18 00:10:38.368 -07 000000547 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:38.368 -07 000000501 INF: FPGA: Recv YRDPOS 143080 17-03-18 00:10:38.378 -07 000000547 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:38.378 -07 000000547 INF: Y Motor: Encoder = 143080 = motor step 111781.3 = 69.8633 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:38.378 -07 000000547 INF: Y Motor: Position error before settling (table move) = -19 encoder counts (-0.009 mm) 17-03-18 00:10:38.378 -07 000000547 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 397 ms, distance = 0.845 mm, average velocity = 2.129 mm/sec 17-03-18 00:10:39.328 -07 000000547 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:39.328 -07 000000012 INF: FPGA: Recv YRDPOS 143080 17-03-18 00:10:39.338 -07 000000547 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:39.338 -07 000000547 INF: Y Motor: Encoder = 143080 = motor step 111781.3 = 69.8633 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:39.358 -07 000000547 INF: Y Motor: After move to 69.872612, error = -0.009331, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:39.358 -07 000000547 INF: Y Motor: Position error after settling (table move) = -19 encoder counts (-0.009 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:39.358 -07 000000547 INF: Y Motor: Moved to 69.8633 after 1462 ms (table move) 17-03-18 00:10:39.368 -07 000000626 INF: Z Motor: EndOfMoveAbsStepped: target = -0.135427 mm (DAC = 29584) 17-03-18 00:10:39.368 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.368 -07 000000476 INF: FPGA: Recv ZSGSRD 30370 17-03-18 00:10:39.378 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:39.378 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.378 -07 000000561 INF: FPGA: Recv ZSGSRD 30373 17-03-18 00:10:39.388 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.388 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.388 -07 000000502 INF: FPGA: Recv ZSGSRD 30365 17-03-18 00:10:39.398 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.398 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.398 -07 000000482 INF: FPGA: Recv ZSGSRD 30376 17-03-18 00:10:39.408 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.408 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.408 -07 000000561 INF: FPGA: Recv ZSGSRD 30371 17-03-18 00:10:39.418 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.418 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.418 -07 000000501 INF: FPGA: Recv ZSGSRD 30374 17-03-18 00:10:39.428 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.428 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.428 -07 000000482 INF: FPGA: Recv ZSGSRD 30372 17-03-18 00:10:39.438 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.438 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.438 -07 000000478 INF: FPGA: Recv ZSGSRD 30360 17-03-18 00:10:39.448 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.448 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.448 -07 000000627 INF: FPGA: Recv ZSGSRD 30377 17-03-18 00:10:39.458 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.458 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:39.458 -07 000000482 INF: FPGA: Recv ZSGSRD 30370 17-03-18 00:10:39.468 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.468 -07 000000626 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:39.468 -07 000000627 INF: FPGA: Recv ZPIEZORD 26119 17-03-18 00:10:39.478 -07 000000626 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:39.478 -07 000000626 INF: Z Motor: Piezo is near 35.8 V (nominally 0 to 100 V), expect near 45.1 V, since DAC is at 4.514 V. Piezo Error = -9.3 V. 17-03-18 00:10:39.478 -07 000000626 INF: Z Motor: ReadAdcAverage: Commanded Z = -135.4 um, Read 10 ADC samples: Avg = 30371 (-134.6 uncal um), Error = 0.8 uncal um, Median = 30372 (-134.6 uncal um), StdDev = 5 (0.0 uncal um), Range = 17 (0.1 uncal um), Min = 30360 (-134.6 uncal um), Max = 30377 (-134.7 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 11 (0.1 uncal um) 17-03-18 00:10:39.478 -07 000000626 INF: Z Motor: ADC noise = 11, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30371, posNoise = 6, negNoise = 11 17-03-18 00:10:39.478 -07 000000626 INF: Z Motor: After move to -0.135427, error = -0.000006, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:39.478 -07 000000626 INF: Z Motor: Settle wait time = 1466 ms, measure time = 108 ms (allowed 150 ms), total time = 1574 ms 17-03-18 00:10:39.478 -07 000000626 INF: Z Motor: Waiting extra 42 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:39.528 -07 000000626 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1629 ms 17-03-18 00:10:39.528 -07 000000626 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:39.528 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:39.528 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:39.528 -07 000000478 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:39.528 -07 000000448 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:39.528 -07 000000627 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:39.528 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:39.538 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:39.538 -07 000000627 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:39.538 -07 000000627 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:39.538 -07 000000627 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:39.548 -07 000000627 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 8 ms. 17-03-18 00:10:39.548 -07 000000627 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 12 ms. 17-03-18 00:10:39.548 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:39.588 -07 000000561 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:39.888 -07 000000482 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:39.888 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:39.898 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:39.898 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1408252120, 0x0, 559484976) start. 17-03-18 00:10:39.928 -07 000000512 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:39.928 -07 000000502 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:39.928 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 379 ms to get response, and 384 ms overall 17-03-18 00:10:40.008 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1408252120, 0x0, 559484976) end. Duration was 115 ms. 17-03-18 00:10:40.008 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:40.008 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:10:40.008 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 118 ms. 17-03-18 00:10:40.028 -07 000000502 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:40.028 -07 000000482 INF: Autofocus start 17-03-18 00:10:40.028 -07 000000482 INF: MeasureProfile start 17-03-18 00:10:40.028 -07 000000512 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:40.038 -07 000000502 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:40.038 -07 000000502 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:40.038 -07 000000482 INF: MeasureProfile done 17-03-18 00:10:40.038 -07 000000482 INF: X=16 Z correction [um]=0.0530179 17-03-18 00:10:40.038 -07 000000482 INF: Autofocus finished 17-03-18 00:10:40.228 -07 000000626 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:10:40.238 -07 000000626 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:40.238 -07 000000502 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 194 ms to get response, and 204 ms overall 17-03-18 00:10:40.238 -07 000000610 INF: ------------ Scan Snapping tile 13 at Y-position 69.86328125, Z-position -0.135433098968557. ---------- 17-03-18 00:10:40.238 -07 000000610 INF: Cycle 82 Tile 13 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:40.248 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28108, sync = True, waitForSettling = True) 17-03-18 00:10:40.248 -07 000000610 INF: Z Motor: Moving to -0.128670 mm, started at -0.135433 mm, distance = 6.76 um 17-03-18 00:10:40.268 -07 000000610 INF: FPGA: Xmit ZDACW 28108\n [Z Motor] 17-03-18 00:10:40.268 -07 000000482 INF: FPGA: Recv ZDACW 17-03-18 00:10:40.278 -07 000000610 INF: FPGA: 'ZDACW 28108' command took 0 ms to send, 0 ms to get response, and 28 ms overall 17-03-18 00:10:40.278 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:40.278 -07 000000502 INF: FPGA: Recv ZDACR 28108 17-03-18 00:10:40.288 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.288 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:40.438 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.438 -07 000000502 INF: FPGA: Recv ZSGSRD 29316 17-03-18 00:10:40.448 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:40.448 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.448 -07 000000626 INF: FPGA: Recv ZSGSRD 29322 17-03-18 00:10:40.458 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.458 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.458 -07 000000482 INF: FPGA: Recv ZSGSRD 29317 17-03-18 00:10:40.468 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.468 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.468 -07 000000626 INF: FPGA: Recv ZSGSRD 29321 17-03-18 00:10:40.478 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.478 -07 000000512 INF: FPGA: Recv ZSGSRD 29324 17-03-18 00:10:40.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.488 -07 000000482 INF: FPGA: Recv ZSGSRD 29314 17-03-18 00:10:40.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.498 -07 000000626 INF: FPGA: Recv ZSGSRD 29322 17-03-18 00:10:40.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.508 -07 000000512 INF: FPGA: Recv ZSGSRD 29320 17-03-18 00:10:40.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.518 -07 000000502 INF: FPGA: Recv ZSGSRD 29321 17-03-18 00:10:40.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:40.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:40.528 -07 000000482 INF: FPGA: Recv ZSGSRD 29317 17-03-18 00:10:40.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.538 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:40.538 -07 000000512 INF: FPGA: Recv ZPIEZORD 24701 17-03-18 00:10:40.548 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:40.548 -07 000000610 INF: Z Motor: Piezo is near 32.8 V (nominally 0 to 100 V), expect near 42.9 V, since DAC is at 4.289 V. Piezo Error = -10.1 V. 17-03-18 00:10:40.548 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -128.7 um, Read 10 ADC samples: Avg = 29319 (-127.9 uncal um), Error = 0.8 uncal um, Median = 29320 (-127.9 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 29314 (-127.9 uncal um), Max = 29324 (-127.9 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:10:40.548 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29319, posNoise = 5, negNoise = 5 17-03-18 00:10:40.548 -07 000000610 INF: Z Motor: After move to -0.128670, error = -0.000005, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:40.548 -07 000000610 INF: Z Motor: MoveAbsStepped took 299 ms) 17-03-18 00:10:40.548 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:40.548 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:40.548 -07 000000626 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:40.548 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:40.548 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:40.548 -07 000000608 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:40.548 -07 000000482 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:40.548 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:40.548 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:40.548 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:40.548 -07 000000482 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:40.548 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:40.548 -07 000000482 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:10:40.548 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:40.558 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:40.558 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:40.558 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 8 ms. 17-03-18 00:10:40.558 -07 000000512 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 13 ms. 17-03-18 00:10:40.558 -07 000000482 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 9 ms. 17-03-18 00:10:40.558 -07 000000482 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 13 ms. 17-03-18 00:10:40.568 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28285 150\n [Camera] 17-03-18 00:10:40.768 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:41.798 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:41.808 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:41.808 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:41.808 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:41.808 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) start. 17-03-18 00:10:41.808 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:41.808 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:41.808 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:41.808 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:41.808 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2182910056, 0x0, 418435064) start. 17-03-18 00:10:41.998 -07 000000482 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28285 1422 17-03-18 00:10:42.138 -07 000000502 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:42.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:42.148 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:42.178 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2182910056, 0x0, 418435064) end. Duration was 375 ms. 17-03-18 00:10:42.178 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 376 ms. 17-03-18 00:10:42.178 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:42.188 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:42.188 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 385 ms. 17-03-18 00:10:42.198 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2147487792, 0x0, 391868352) end. Duration was 389 ms. 17-03-18 00:10:42.198 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 389 ms. 17-03-18 00:10:42.198 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:42.198 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:42.198 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 394 ms. 17-03-18 00:10:42.648 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:42.648 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:42.648 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:42.648 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:42.648 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2218332320, 0x0, 391868352) start. 17-03-18 00:10:42.648 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:42.648 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:42.648 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:42.648 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:42.648 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2281705520, 0x0, 418435064) start. 17-03-18 00:10:42.838 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:42.838 -07 000000626 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:42.838 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28285 150' command took 2 ms to send, 2264 ms to get response, and 2272 ms overall 17-03-18 00:10:42.838 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:42.838 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 14 at Y-position 69.86328125, Z-Position -0.128674827795425. ------ 17-03-18 00:10:42.848 -07 000000626 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:42.848 -07 000000482 INF: Y Motor: MoveYZ optimized starting. Moving Y to 70.7026 mm, moving Z to -0.13477 mm 17-03-18 00:10:42.848 -07 000000482 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:42.848 -07 000000569 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:42.848 -07 000000382 INF: Y Motor: Entering MoveAbsStepped (target = 113124 steps, sync = True, waitForSettling = True) 17-03-18 00:10:42.848 -07 000000382 INF: Y Motor: Moving to 70.7026 mm, started at 69.8633 mm, delta = 0.8393 mm 17-03-18 00:10:42.848 -07 000000502 INF: Z Motor: Entering MoveAbsStepped (DAC = 29441, sync = True, waitForSettling = False) 17-03-18 00:10:42.848 -07 000000502 INF: Z Motor: Moving to -0.134772 mm, started at -0.128675 mm, distance = -6.10 um 17-03-18 00:10:42.858 -07 000000626 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:42.858 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:42.858 -07 000000586 INF: FPGA: Recv YRDPOS 143080 17-03-18 00:10:42.868 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 15 ms overall 17-03-18 00:10:42.868 -07 000000382 INF: Y Motor: Encoder = 143080 = motor step 111781.3 = 69.8633 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:42.868 -07 000000382 INF: Y Motor: Last commanded position = 69.8726 mm (111796.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:42.868 -07 000000382 INF: Y Motor: Moving 1343 steps-19 17-03-18 00:10:42.868 -07 000000502 INF: FPGA: Xmit ZDACW 29441\n [Z Motor] 17-03-18 00:10:42.868 -07 000000438 INF: FPGA: Recv ZDACW 17-03-18 00:10:42.878 -07 000000626 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:42.878 -07 000000502 INF: FPGA: 'ZDACW 29441' command took 1 ms to send, 0 ms to get response, and 25 ms overall 17-03-18 00:10:42.888 -07 000000382 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:42.898 -07 000000502 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:42.898 -07 000000424 INF: FPGA: Recv ZDACR 29441 17-03-18 00:10:42.908 -07 000000502 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:10:42.908 -07 000000502 INF: Z Motor: MoveAbsStepped took 55 ms (did NOT wait for settling yet)) 17-03-18 00:10:43.048 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2218332320, 0x0, 391868352) end. Duration was 404 ms. 17-03-18 00:10:43.048 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 404 ms. 17-03-18 00:10:43.048 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:43.048 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2281705520, 0x0, 418435064) end. Duration was 402 ms. 17-03-18 00:10:43.048 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 403 ms. 17-03-18 00:10:43.048 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:43.058 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:43.058 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:10:43.058 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 412 ms. 17-03-18 00:10:43.058 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 413 ms. 17-03-18 00:10:43.078 -07 000000502 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:43.078 -07 000000626 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 192 ms to get response, and 222 ms overall 17-03-18 00:10:43.258 -07 000000569 INF: FPGA: Recv YMVTILE 17-03-18 00:10:43.268 -07 000000382 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 369 ms to get response, and 399 ms overall 17-03-18 00:10:43.308 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:43.308 -07 000000512 INF: FPGA: Recv YRDPOS 144770 17-03-18 00:10:43.318 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:43.318 -07 000000382 INF: Y Motor: Encoder = 144770 = motor step 113101.6 = 70.6885 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:43.318 -07 000000382 INF: Y Motor: Position error before settling (table move) = -29 encoder counts (-0.014 mm) 17-03-18 00:10:43.318 -07 000000382 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 399 ms, distance = 0.839 mm, average velocity = 2.104 mm/sec 17-03-18 00:10:44.268 -07 000000382 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:44.268 -07 000000626 INF: FPGA: Recv YRDPOS 144770 17-03-18 00:10:44.278 -07 000000382 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.278 -07 000000382 INF: Y Motor: Encoder = 144770 = motor step 113101.6 = 70.6885 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:44.298 -07 000000382 INF: Y Motor: After move to 70.702606, error = -0.014130, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:44.298 -07 000000382 INF: Y Motor: Position error after settling (table move) = -29 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:44.298 -07 000000382 INF: Y Motor: Moved to 70.6885 after 1454 ms (table move) 17-03-18 00:10:44.308 -07 000000482 INF: Z Motor: EndOfMoveAbsStepped: target = -0.134772 mm (DAC = 29441) 17-03-18 00:10:44.308 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.308 -07 000000382 INF: FPGA: Recv ZSGSRD 30271 17-03-18 00:10:44.318 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:44.318 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.318 -07 000000382 INF: FPGA: Recv ZSGSRD 30278 17-03-18 00:10:44.328 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.328 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.328 -07 000000382 INF: FPGA: Recv ZSGSRD 30268 17-03-18 00:10:44.338 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.338 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.338 -07 000000561 INF: FPGA: Recv ZSGSRD 30266 17-03-18 00:10:44.348 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.348 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.348 -07 000000586 INF: FPGA: Recv ZSGSRD 30266 17-03-18 00:10:44.358 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.358 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.358 -07 000000586 INF: FPGA: Recv ZSGSRD 30268 17-03-18 00:10:44.368 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.368 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.368 -07 000000586 INF: FPGA: Recv ZSGSRD 30268 17-03-18 00:10:44.378 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.378 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.378 -07 000000586 INF: FPGA: Recv ZSGSRD 30267 17-03-18 00:10:44.388 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.388 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.388 -07 000000626 INF: FPGA: Recv ZSGSRD 30268 17-03-18 00:10:44.398 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.398 -07 000000482 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:44.398 -07 000000586 INF: FPGA: Recv ZSGSRD 30274 17-03-18 00:10:44.408 -07 000000482 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.408 -07 000000482 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:44.408 -07 000000586 INF: FPGA: Recv ZPIEZORD 26000 17-03-18 00:10:44.418 -07 000000482 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:44.418 -07 000000482 INF: Z Motor: Piezo is near 35.5 V (nominally 0 to 100 V), expect near 44.9 V, since DAC is at 4.492 V. Piezo Error = -9.4 V. 17-03-18 00:10:44.418 -07 000000482 INF: Z Motor: ReadAdcAverage: Commanded Z = -134.8 um, Read 10 ADC samples: Avg = 30269 (-134.0 uncal um), Error = 0.8 uncal um, Median = 30268 (-134.0 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 30266 (-134.0 uncal um), Max = 30278 (-134.0 uncal um), Pos Noise = 9 (0.1 uncal um), Neg Noise = 3 (0.0 uncal um) 17-03-18 00:10:44.418 -07 000000482 INF: Z Motor: ADC noise = 9, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30269, posNoise = 9, negNoise = 3 17-03-18 00:10:44.418 -07 000000482 INF: Z Motor: After move to -0.134772, error = -0.000006, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:44.418 -07 000000482 INF: Z Motor: Settle wait time = 1457 ms, measure time = 110 ms (allowed 150 ms), total time = 1567 ms 17-03-18 00:10:44.418 -07 000000482 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:44.458 -07 000000482 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1608 ms 17-03-18 00:10:44.458 -07 000000482 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:44.458 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:44.458 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:44.458 -07 000000512 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:44.458 -07 000000586 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:44.458 -07 000000626 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:44.458 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:44.458 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:44.458 -07 000000626 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:44.468 -07 000000626 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:44.468 -07 000000626 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:44.468 -07 000000626 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 6 ms. 17-03-18 00:10:44.468 -07 000000626 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 12 ms. 17-03-18 00:10:44.478 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:44.518 -07 000000424 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:44.818 -07 000000438 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:44.818 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:44.818 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:44.818 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1201016592, 0x0, 559484976) start. 17-03-18 00:10:44.858 -07 000000626 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:44.858 -07 000000438 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:44.858 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 377 ms to get response, and 384 ms overall 17-03-18 00:10:44.948 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 1201016592, 0x0, 559484976) end. Duration was 127 ms. 17-03-18 00:10:44.948 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:44.948 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:10:44.948 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 129 ms. 17-03-18 00:10:45.058 -07 000000512 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:45.058 -07 000000502 INF: Autofocus start 17-03-18 00:10:45.058 -07 000000502 INF: MeasureProfile start 17-03-18 00:10:45.058 -07 000000608 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:45.068 -07 000000512 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:10:45.068 -07 000000512 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:45.068 -07 000000502 INF: MeasureProfile done 17-03-18 00:10:45.068 -07 000000502 INF: X=96 Z correction [um]=0.437849 17-03-18 00:10:45.068 -07 000000502 INF: Autofocus finished 17-03-18 00:10:45.268 -07 000000626 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:45.268 -07 000000512 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 195 ms to get response, and 204 ms overall 17-03-18 00:10:45.268 -07 000000610 INF: ------------ Scan Snapping tile 14 at Y-position 70.6884765625, Z-position -0.134777829330097. ---------- 17-03-18 00:10:45.278 -07 000000610 INF: Cycle 82 Tile 14 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:45.278 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28049, sync = True, waitForSettling = True) 17-03-18 00:10:45.278 -07 000000610 INF: Z Motor: Moving to -0.128400 mm, started at -0.134778 mm, distance = 6.38 um 17-03-18 00:10:45.298 -07 000000610 INF: FPGA: Xmit ZDACW 28049\n [Z Motor] 17-03-18 00:10:45.298 -07 000000502 INF: FPGA: Recv ZDACW 17-03-18 00:10:45.308 -07 000000610 INF: FPGA: 'ZDACW 28049' command took 0 ms to send, 0 ms to get response, and 27 ms overall 17-03-18 00:10:45.308 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:45.308 -07 000000608 INF: FPGA: Recv ZDACR 28049 17-03-18 00:10:45.318 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.318 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:45.468 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.468 -07 000000608 INF: FPGA: Recv ZSGSRD 29281 17-03-18 00:10:45.478 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:45.478 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.478 -07 000000502 INF: FPGA: Recv ZSGSRD 29279 17-03-18 00:10:45.488 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.488 -07 000000502 INF: FPGA: Recv ZSGSRD 29273 17-03-18 00:10:45.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.498 -07 000000502 INF: FPGA: Recv ZSGSRD 29277 17-03-18 00:10:45.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.508 -07 000000502 INF: FPGA: Recv ZSGSRD 29278 17-03-18 00:10:45.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.518 -07 000000512 INF: FPGA: Recv ZSGSRD 29285 17-03-18 00:10:45.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.528 -07 000000569 INF: FPGA: Recv ZSGSRD 29277 17-03-18 00:10:45.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.538 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.538 -07 000000502 INF: FPGA: Recv ZSGSRD 29273 17-03-18 00:10:45.548 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.548 -07 000000608 INF: FPGA: Recv ZSGSRD 29278 17-03-18 00:10:45.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:45.558 -07 000000512 INF: FPGA: Recv ZSGSRD 29277 17-03-18 00:10:45.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.568 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:45.568 -07 000000512 INF: FPGA: Recv ZPIEZORD 24667 17-03-18 00:10:45.578 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:45.578 -07 000000610 INF: Z Motor: Piezo is near 32.7 V (nominally 0 to 100 V), expect near 42.8 V, since DAC is at 4.280 V. Piezo Error = -10.1 V. 17-03-18 00:10:45.578 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -128.4 um, Read 10 ADC samples: Avg = 29278 (-127.6 uncal um), Error = 0.8 uncal um, Median = 29278 (-127.6 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 29273 (-127.6 uncal um), Max = 29285 (-127.7 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:10:45.578 -07 000000610 INF: Z Motor: ADC noise = 7, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29278, posNoise = 7, negNoise = 5 17-03-18 00:10:45.578 -07 000000610 INF: Z Motor: After move to -0.128400, error = -0.000011, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:45.578 -07 000000610 INF: Z Motor: MoveAbsStepped took 298 ms) 17-03-18 00:10:45.578 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:45.578 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:45.578 -07 000000502 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:45.578 -07 000000512 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:45.578 -07 000000569 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:45.578 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:45.578 -07 000000608 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:45.578 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:45.578 -07 000000569 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:45.578 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:45.578 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:45.578 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:45.578 -07 000000569 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:45.578 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:45.588 -07 000000608 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:10:45.588 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:45.598 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:10:45.598 -07 000000569 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:10:45.598 -07 000000569 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:10:45.598 -07 000000608 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 14 ms. 17-03-18 00:10:45.598 -07 000000608 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:10:45.608 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28226 150\n [Camera] 17-03-18 00:10:45.798 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:46.838 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:46.838 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:46.838 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:46.838 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:46.838 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2317127784, 0x0, 391868352) start. 17-03-18 00:10:46.848 -07 000000569 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:46.848 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:46.848 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:46.848 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:46.848 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 2352550048, 0x0, 418435064) start. 17-03-18 00:10:47.038 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28226 1422 17-03-18 00:10:47.138 -07 000000012 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:47.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:47.188 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:47.238 -07 000000608 INF: Camera: sBCa_ReadImage(785074288, 2317127784, 0x0, 391868352) end. Duration was 399 ms. 17-03-18 00:10:47.238 -07 000000608 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:10:47.238 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:47.248 -07 000000478 INF: Camera: sBCa_ReadImage(785074704, 2352550048, 0x0, 418435064) end. Duration was 399 ms. 17-03-18 00:10:47.248 -07 000000478 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:10:47.248 -07 000000478 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:47.248 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:47.248 -07 000000608 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 404 ms. 17-03-18 00:10:47.248 -07 000000478 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:47.258 -07 000000478 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:10:47.688 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:47.688 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:47.688 -07 000000502 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:47.688 -07 000000502 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:47.688 -07 000000502 INF: Camera: sBCa_ReadImage(785074288, 2415923248, 0x0, 391868352) start. 17-03-18 00:10:47.698 -07 000000569 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:47.698 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:47.698 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:47.698 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:47.698 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2451345512, 0x0, 418435064) start. 17-03-18 00:10:47.878 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP 2264 17-03-18 00:10:47.878 -07 000000569 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:47.878 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28226 150' command took 2 ms to send, 2262 ms to get response, and 2274 ms overall 17-03-18 00:10:47.878 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:47.878 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 15 at Y-position 70.6884765625, Z-Position -0.128411435097612. ------ 17-03-18 00:10:47.878 -07 000000486 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:47.878 -07 000000547 INF: Y Motor: MoveYZ optimized starting. Moving Y to 71.5326 mm, moving Z to -0.13487 mm 17-03-18 00:10:47.878 -07 000000547 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:47.878 -07 000000012 INF: Z Motor: Entering MoveAbsStepped (DAC = 29462, sync = True, waitForSettling = False) 17-03-18 00:10:47.878 -07 000000012 INF: Z Motor: Moving to -0.134868 mm, started at -0.128411 mm, distance = -6.46 um 17-03-18 00:10:47.878 -07 000000512 INF: Y Motor: Entering MoveAbsStepped (target = 114452 steps, sync = True, waitForSettling = True) 17-03-18 00:10:47.888 -07 000000512 INF: Y Motor: Moving to 71.5326 mm, started at 70.6885 mm, delta = 0.8441 mm 17-03-18 00:10:47.888 -07 000000512 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:47.888 -07 000000501 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:47.888 -07 000000486 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 6 ms to get response, and 9 ms overall 17-03-18 00:10:47.888 -07 000000478 INF: FPGA: Recv YRDPOS 144770 17-03-18 00:10:47.898 -07 000000512 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:47.898 -07 000000512 INF: Y Motor: Encoder = 144770 = motor step 113101.6 = 70.6885 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:47.898 -07 000000512 INF: Y Motor: Last commanded position = 70.7026 mm (113124.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:47.898 -07 000000512 INF: Y Motor: Moving 1351 steps-29 17-03-18 00:10:47.898 -07 000000486 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:47.908 -07 000000012 INF: FPGA: Xmit ZDACW 29462\n [Z Motor] 17-03-18 00:10:47.908 -07 000000627 INF: FPGA: Recv ZDACW 17-03-18 00:10:47.918 -07 000000012 INF: FPGA: 'ZDACW 29462' command took 0 ms to send, 0 ms to get response, and 30 ms overall 17-03-18 00:10:47.918 -07 000000512 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:47.928 -07 000000012 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:47.928 -07 000000627 INF: FPGA: Recv ZDACR 29462 17-03-18 00:10:47.938 -07 000000012 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:10:47.938 -07 000000012 INF: Z Motor: MoveAbsStepped took 50 ms (did NOT wait for settling yet)) 17-03-18 00:10:48.078 -07 000000608 INF: Camera: sBCa_ReadImage(785074704, 2451345512, 0x0, 418435064) end. Duration was 387 ms. 17-03-18 00:10:48.078 -07 000000608 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 388 ms. 17-03-18 00:10:48.078 -07 000000608 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:48.088 -07 000000502 INF: Camera: sBCa_ReadImage(785074288, 2415923248, 0x0, 391868352) end. Duration was 395 ms. 17-03-18 00:10:48.088 -07 000000502 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 395 ms. 17-03-18 00:10:48.088 -07 000000502 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:48.088 -07 000000608 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:48.088 -07 000000502 INF: Camera: CopyActivePixFullImage end. Duration was 6 ms. 17-03-18 00:10:48.088 -07 000000502 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:10:48.088 -07 000000608 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 398 ms. 17-03-18 00:10:48.098 -07 000000569 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:48.098 -07 000000486 INF: FPGA: 'FOCMRRFOC' command took 1 ms to send, 193 ms to get response, and 207 ms overall 17-03-18 00:10:48.288 -07 000000501 INF: FPGA: Recv YMVTILE 17-03-18 00:10:48.288 -07 000000512 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 398 ms overall 17-03-18 00:10:48.338 -07 000000512 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:48.338 -07 000000448 INF: FPGA: Recv YRDPOS 146471 17-03-18 00:10:48.348 -07 000000512 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:48.348 -07 000000512 INF: Y Motor: Encoder = 146471 = motor step 114430.5 = 71.5190 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:48.348 -07 000000512 INF: Y Motor: Position error before settling (table move) = -28 encoder counts (-0.014 mm) 17-03-18 00:10:48.348 -07 000000512 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 398 ms, distance = 0.844 mm, average velocity = 2.121 mm/sec 17-03-18 00:10:49.298 -07 000000512 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:49.298 -07 000000569 INF: FPGA: Recv YRDPOS 146471 17-03-18 00:10:49.308 -07 000000512 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:49.308 -07 000000512 INF: Y Motor: Encoder = 146471 = motor step 114430.5 = 71.5190 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:49.328 -07 000000512 INF: Y Motor: After move to 71.532608, error = -0.013565, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:49.328 -07 000000512 INF: Y Motor: Position error after settling (table move) = -28 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:49.328 -07 000000512 INF: Y Motor: Moved to 71.5190 after 1448 ms (table move) 17-03-18 00:10:49.338 -07 000000547 INF: Z Motor: EndOfMoveAbsStepped: target = -0.134868 mm (DAC = 29462) 17-03-18 00:10:49.338 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.338 -07 000000486 INF: FPGA: Recv ZSGSRD 30281 17-03-18 00:10:49.348 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:49.348 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.348 -07 000000502 INF: FPGA: Recv ZSGSRD 30280 17-03-18 00:10:49.358 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.358 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.358 -07 000000478 INF: FPGA: Recv ZSGSRD 30289 17-03-18 00:10:49.368 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.368 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.368 -07 000000478 INF: FPGA: Recv ZSGSRD 30287 17-03-18 00:10:49.378 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.378 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.378 -07 000000478 INF: FPGA: Recv ZSGSRD 30285 17-03-18 00:10:49.388 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.388 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.388 -07 000000502 INF: FPGA: Recv ZSGSRD 30279 17-03-18 00:10:49.398 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.398 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.398 -07 000000627 INF: FPGA: Recv ZSGSRD 30284 17-03-18 00:10:49.408 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.408 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.408 -07 000000448 INF: FPGA: Recv ZSGSRD 30282 17-03-18 00:10:49.418 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.418 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.418 -07 000000627 INF: FPGA: Recv ZSGSRD 30273 17-03-18 00:10:49.428 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.428 -07 000000547 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:49.428 -07 000000486 INF: FPGA: Recv ZSGSRD 30282 17-03-18 00:10:49.438 -07 000000547 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.438 -07 000000547 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:49.438 -07 000000502 INF: FPGA: Recv ZPIEZORD 26025 17-03-18 00:10:49.448 -07 000000547 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:49.448 -07 000000547 INF: Z Motor: Piezo is near 35.6 V (nominally 0 to 100 V), expect near 45.0 V, since DAC is at 4.496 V. Piezo Error = -9.4 V. 17-03-18 00:10:49.448 -07 000000547 INF: Z Motor: ReadAdcAverage: Commanded Z = -134.9 um, Read 10 ADC samples: Avg = 30282 (-134.1 uncal um), Error = 0.8 uncal um, Median = 30282 (-134.1 uncal um), StdDev = 4 (0.0 uncal um), Range = 16 (0.1 uncal um), Min = 30273 (-134.0 uncal um), Max = 30289 (-134.1 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 9 (0.1 uncal um) 17-03-18 00:10:49.448 -07 000000547 INF: Z Motor: ADC noise = 9, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30282, posNoise = 7, negNoise = 9 17-03-18 00:10:49.448 -07 000000547 INF: Z Motor: After move to -0.134868, error = 0.000007, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:49.448 -07 000000547 INF: Z Motor: Settle wait time = 1451 ms, measure time = 109 ms (allowed 150 ms), total time = 1560 ms 17-03-18 00:10:49.448 -07 000000547 INF: Z Motor: Waiting extra 41 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:49.498 -07 000000547 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1613 ms 17-03-18 00:10:49.498 -07 000000547 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:49.498 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:49.498 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:49.498 -07 000000476 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:49.498 -07 000000501 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:49.498 -07 000000608 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:49.498 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:49.498 -07 000000608 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 2 ms. 17-03-18 00:10:49.498 -07 000000608 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:49.508 -07 000000608 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:49.508 -07 000000608 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:49.518 -07 000000608 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 11 ms. 17-03-18 00:10:49.518 -07 000000608 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 16 ms. 17-03-18 00:10:49.518 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:49.558 -07 000000512 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:10:49.858 -07 000000569 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:10:49.858 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:10:49.868 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:49.868 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2387972312, 0x0, 559484976) start. 17-03-18 00:10:49.898 -07 000000476 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:49.898 -07 000000502 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:49.898 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 379 ms to get response, and 384 ms overall 17-03-18 00:10:49.988 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2387972312, 0x0, 559484976) end. Duration was 127 ms. 17-03-18 00:10:49.988 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:49.998 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:10:49.998 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 130 ms. 17-03-18 00:10:50.098 -07 000000501 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:50.098 -07 000000448 INF: Autofocus start 17-03-18 00:10:50.098 -07 000000448 INF: MeasureProfile start 17-03-18 00:10:50.098 -07 000000512 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:50.108 -07 000000501 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:10:50.108 -07 000000501 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:50.108 -07 000000448 INF: MeasureProfile done 17-03-18 00:10:50.108 -07 000000448 INF: X=48 Z correction [um]=0.2069503 17-03-18 00:10:50.108 -07 000000448 INF: Autofocus finished 17-03-18 00:10:50.308 -07 000000478 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:50.308 -07 000000501 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:50.308 -07 000000610 INF: ------------ Scan Snapping tile 15 at Y-position 71.51904296875, Z-position -0.13486134408794. ---------- 17-03-18 00:10:50.308 -07 000000610 INF: Cycle 82 Tile 15 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:50.308 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 28020, sync = True, waitForSettling = True) 17-03-18 00:10:50.308 -07 000000610 INF: Z Motor: Moving to -0.128267 mm, started at -0.134861 mm, distance = 6.59 um 17-03-18 00:10:50.318 -07 000000610 INF: FPGA: Xmit ZDACW 28020\n [Z Motor] 17-03-18 00:10:50.318 -07 000000012 INF: FPGA: Recv ZDACW 17-03-18 00:10:50.328 -07 000000610 INF: FPGA: 'ZDACW 28020' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:50.328 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:50.328 -07 000000448 INF: FPGA: Recv ZDACR 28020 17-03-18 00:10:50.338 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.338 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:50.488 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.488 -07 000000012 INF: FPGA: Recv ZSGSRD 29255 17-03-18 00:10:50.498 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:50.498 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.498 -07 000000448 INF: FPGA: Recv ZSGSRD 29257 17-03-18 00:10:50.508 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.508 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.508 -07 000000501 INF: FPGA: Recv ZSGSRD 29255 17-03-18 00:10:50.518 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.518 -07 000000502 INF: FPGA: Recv ZSGSRD 29259 17-03-18 00:10:50.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.528 -07 000000012 INF: FPGA: Recv ZSGSRD 29256 17-03-18 00:10:50.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.538 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.538 -07 000000502 INF: FPGA: Recv ZSGSRD 29256 17-03-18 00:10:50.548 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.548 -07 000000502 INF: FPGA: Recv ZSGSRD 29259 17-03-18 00:10:50.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.558 -07 000000502 INF: FPGA: Recv ZSGSRD 29256 17-03-18 00:10:50.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.568 -07 000000448 INF: FPGA: Recv ZSGSRD 29260 17-03-18 00:10:50.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.578 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:50.578 -07 000000448 INF: FPGA: Recv ZSGSRD 29261 17-03-18 00:10:50.588 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.588 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:50.588 -07 000000448 INF: FPGA: Recv ZPIEZORD 24639 17-03-18 00:10:50.598 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:50.598 -07 000000610 INF: Z Motor: Piezo is near 32.6 V (nominally 0 to 100 V), expect near 42.8 V, since DAC is at 4.276 V. Piezo Error = -10.1 V. 17-03-18 00:10:50.598 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -128.3 um, Read 10 ADC samples: Avg = 29257 (-127.5 uncal um), Error = 0.8 uncal um, Median = 29256 (-127.5 uncal um), StdDev = 2 (0.0 uncal um), Range = 6 (0.0 uncal um), Min = 29255 (-127.5 uncal um), Max = 29261 (-127.5 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 2 (0.0 uncal um) 17-03-18 00:10:50.598 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29257, posNoise = 4, negNoise = 2 17-03-18 00:10:50.598 -07 000000610 INF: Z Motor: After move to -0.128267, error = -0.000009, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:50.598 -07 000000610 INF: Z Motor: MoveAbsStepped took 281 ms) 17-03-18 00:10:50.598 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:50.598 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:50.598 -07 000000448 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:50.598 -07 000000502 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:50.598 -07 000000448 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:50.598 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:50.598 -07 000000502 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:50.598 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:50.598 -07 000000448 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:50.598 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:50.598 -07 000000502 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:10:50.598 -07 000000502 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:50.608 -07 000000448 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:50.608 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:50.608 -07 000000502 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 4 ms. 17-03-18 00:10:50.608 -07 000000502 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:50.618 -07 000000448 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:10:50.618 -07 000000448 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:10:50.618 -07 000000502 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:10:50.618 -07 000000502 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:50.628 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28197 150\n [Camera] 17-03-18 00:10:50.828 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:50.978 -07 000000502 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:10:51.858 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:51.858 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:51.868 -07 000000502 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:51.868 -07 000000502 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:51.868 -07 000000502 INF: Camera: sBCa_ReadImage(785074288, 2486767776, 0x0, 391868352) start. 17-03-18 00:10:51.868 -07 000000448 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:51.868 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:51.868 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:51.868 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:51.868 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 2550140976, 0x0, 418435064) start. 17-03-18 00:10:52.058 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28197 1422 17-03-18 00:10:52.138 -07 000000561 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:52.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:52.208 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:52.268 -07 000000502 INF: Camera: sBCa_ReadImage(785074288, 2486767776, 0x0, 391868352) end. Duration was 400 ms. 17-03-18 00:10:52.268 -07 000000502 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 402 ms. 17-03-18 00:10:52.268 -07 000000502 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:52.268 -07 000000482 INF: Camera: sBCa_ReadImage(785074704, 2550140976, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:10:52.268 -07 000000482 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 401 ms. 17-03-18 00:10:52.268 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:52.268 -07 000000502 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:10:52.268 -07 000000502 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 407 ms. 17-03-18 00:10:52.268 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:52.278 -07 000000482 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 412 ms. 17-03-18 00:10:52.708 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:52.708 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:52.708 -07 000000482 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:52.708 -07 000000482 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:52.708 -07 000000482 INF: Camera: sBCa_ReadImage(785074288, 2585563240, 0x0, 391868352) start. 17-03-18 00:10:52.708 -07 000000502 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:52.708 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:52.708 -07 000000438 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:52.708 -07 000000438 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:52.708 -07 000000438 INF: Camera: sBCa_ReadImage(785074704, 2620985504, 0x0, 418435064) start. 17-03-18 00:10:52.898 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:52.898 -07 000000448 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:52.898 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28197 150' command took 2 ms to send, 2264 ms to get response, and 2272 ms overall 17-03-18 00:10:52.898 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:52.898 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 16 at Y-position 71.51904296875, Z-Position -0.128276526642635. ------ 17-03-18 00:10:52.898 -07 000000586 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:52.898 -07 000000626 INF: Y Motor: MoveYZ optimized starting. Moving Y to 72.3626 mm, moving Z to -0.13470 mm 17-03-18 00:10:52.898 -07 000000626 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:52.908 -07 000000424 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:52.908 -07 000000586 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 2 ms to get response, and 4 ms overall 17-03-18 00:10:52.908 -07 000000586 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:52.908 -07 000000502 INF: Z Motor: Entering MoveAbsStepped (DAC = 29426, sync = True, waitForSettling = False) 17-03-18 00:10:52.908 -07 000000502 INF: Z Motor: Moving to -0.134704 mm, started at -0.128277 mm, distance = -6.43 um 17-03-18 00:10:52.908 -07 000000448 INF: Y Motor: Entering MoveAbsStepped (target = 115780 steps, sync = True, waitForSettling = True) 17-03-18 00:10:52.908 -07 000000448 INF: Y Motor: Moving to 72.3626 mm, started at 71.5190 mm, delta = 0.8436 mm 17-03-18 00:10:52.918 -07 000000502 INF: FPGA: Xmit ZDACW 29426\n [Z Motor] 17-03-18 00:10:52.918 -07 000000627 INF: FPGA: Recv ZDACW 17-03-18 00:10:52.928 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:52.928 -07 000000502 INF: FPGA: 'ZDACW 29426' command took 0 ms to send, 0 ms to get response, and 15 ms overall 17-03-18 00:10:52.928 -07 000000478 INF: FPGA: Recv YRDPOS 146471 17-03-18 00:10:52.938 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 25 ms overall 17-03-18 00:10:52.938 -07 000000448 INF: Y Motor: Encoder = 146471 = motor step 114430.5 = 71.5190 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:52.938 -07 000000448 INF: Y Motor: Last commanded position = 71.5326 mm (114452.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:52.938 -07 000000448 INF: Y Motor: Moving 1350 steps-28 17-03-18 00:10:52.938 -07 000000502 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:52.938 -07 000000627 INF: FPGA: Recv ZDACR 29426 17-03-18 00:10:52.948 -07 000000502 INF: FPGA: 'ZDACR' command took 1 ms to send, 0 ms to get response, and 19 ms overall 17-03-18 00:10:52.948 -07 000000502 INF: Z Motor: MoveAbsStepped took 35 ms (did NOT wait for settling yet)) 17-03-18 00:10:52.948 -07 000000448 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:53.108 -07 000000502 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:53.108 -07 000000586 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 196 ms to get response, and 202 ms overall 17-03-18 00:10:53.108 -07 000000482 INF: Camera: sBCa_ReadImage(785074288, 2585563240, 0x0, 391868352) end. Duration was 402 ms. 17-03-18 00:10:53.108 -07 000000482 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 403 ms. 17-03-18 00:10:53.108 -07 000000482 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:53.108 -07 000000438 INF: Camera: sBCa_ReadImage(785074704, 2620985504, 0x0, 418435064) end. Duration was 402 ms. 17-03-18 00:10:53.108 -07 000000438 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 402 ms. 17-03-18 00:10:53.108 -07 000000438 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:53.118 -07 000000438 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:10:53.118 -07 000000482 INF: Camera: CopyActivePixFullImage end. Duration was 7 ms. 17-03-18 00:10:53.118 -07 000000482 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 411 ms. 17-03-18 00:10:53.118 -07 000000438 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 413 ms. 17-03-18 00:10:53.318 -07 000000627 INF: FPGA: Recv YMVTILE 17-03-18 00:10:53.318 -07 000000448 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 387 ms overall 17-03-18 00:10:53.368 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:53.368 -07 000000608 INF: FPGA: Recv YRDPOS 148172 17-03-18 00:10:53.378 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:53.378 -07 000000448 INF: Y Motor: Encoder = 148172 = motor step 115759.4 = 72.3496 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:53.378 -07 000000448 INF: Y Motor: Position error before settling (table move) = -27 encoder counts (-0.013 mm) 17-03-18 00:10:53.378 -07 000000448 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 388 ms, distance = 0.844 mm, average velocity = 2.174 mm/sec 17-03-18 00:10:54.328 -07 000000448 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:54.328 -07 000000608 INF: FPGA: Recv YRDPOS 148172 17-03-18 00:10:54.338 -07 000000448 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:54.338 -07 000000448 INF: Y Motor: Encoder = 148172 = motor step 115759.4 = 72.3496 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:54.358 -07 000000448 INF: Y Motor: After move to 72.362610, error = -0.013000, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:54.358 -07 000000448 INF: Y Motor: Position error after settling (table move) = -27 encoder counts (-0.013 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:54.358 -07 000000448 INF: Y Motor: Moved to 72.3496 after 1454 ms (table move) 17-03-18 00:10:54.368 -07 000000626 INF: Z Motor: EndOfMoveAbsStepped: target = -0.134704 mm (DAC = 29426) 17-03-18 00:10:54.368 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.368 -07 000000501 INF: FPGA: Recv ZSGSRD 30255 17-03-18 00:10:54.378 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:54.378 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.378 -07 000000627 INF: FPGA: Recv ZSGSRD 30256 17-03-18 00:10:54.388 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.388 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.388 -07 000000382 INF: FPGA: Recv ZSGSRD 30252 17-03-18 00:10:54.398 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.398 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.398 -07 000000502 INF: FPGA: Recv ZSGSRD 30262 17-03-18 00:10:54.408 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.408 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.408 -07 000000627 INF: FPGA: Recv ZSGSRD 30260 17-03-18 00:10:54.418 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.418 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.418 -07 000000424 INF: FPGA: Recv ZSGSRD 30260 17-03-18 00:10:54.428 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.428 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.428 -07 000000502 INF: FPGA: Recv ZSGSRD 30258 17-03-18 00:10:54.438 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.438 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.438 -07 000000448 INF: FPGA: Recv ZSGSRD 30256 17-03-18 00:10:54.448 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.448 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.448 -07 000000424 INF: FPGA: Recv ZSGSRD 30256 17-03-18 00:10:54.458 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.458 -07 000000626 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:54.458 -07 000000502 INF: FPGA: Recv ZSGSRD 30259 17-03-18 00:10:54.468 -07 000000626 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.468 -07 000000626 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:54.468 -07 000000608 INF: FPGA: Recv ZPIEZORD 25981 17-03-18 00:10:54.478 -07 000000626 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:54.478 -07 000000626 INF: Z Motor: Piezo is near 35.5 V (nominally 0 to 100 V), expect near 44.9 V, since DAC is at 4.490 V. Piezo Error = -9.4 V. 17-03-18 00:10:54.478 -07 000000626 INF: Z Motor: ReadAdcAverage: Commanded Z = -134.7 um, Read 10 ADC samples: Avg = 30257 (-133.9 uncal um), Error = 0.8 uncal um, Median = 30257 (-133.9 uncal um), StdDev = 3 (0.0 uncal um), Range = 10 (0.1 uncal um), Min = 30252 (-133.9 uncal um), Max = 30262 (-133.9 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:10:54.478 -07 000000626 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30257, posNoise = 5, negNoise = 5 17-03-18 00:10:54.478 -07 000000626 INF: Z Motor: After move to -0.134704, error = 0.000003, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:54.478 -07 000000626 INF: Z Motor: Settle wait time = 1458 ms, measure time = 108 ms (allowed 150 ms), total time = 1566 ms 17-03-18 00:10:54.478 -07 000000626 INF: Z Motor: Waiting extra 42 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:54.528 -07 000000626 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1621 ms 17-03-18 00:10:54.528 -07 000000626 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:54.528 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:54.528 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:54.528 -07 000000478 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:54.528 -07 000000501 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:54.528 -07 000000561 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:10:54.528 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:54.528 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 0 ms. 17-03-18 00:10:54.528 -07 000000561 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:10:54.538 -07 000000561 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:10:54.538 -07 000000561 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:10:54.538 -07 000000561 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 7 ms. 17-03-18 00:10:54.538 -07 000000561 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 11 ms. 17-03-18 00:10:54.548 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:10:54.588 -07 000000626 INF: FPGA: Recv @CAMSNAP turning led on 42 17-03-18 00:10:54.888 -07 000000561 INF: FPGA: Recv @CAMSNAP img rdy 342 17-03-18 00:10:54.888 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 342 17-03-18 00:10:54.888 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:10:54.888 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2402648392, 0x0, 559484976) start. 17-03-18 00:10:54.928 -07 000000482 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:10:54.928 -07 000000438 INF: FPGA: Recv CAMSNAP 17-03-18 00:10:54.928 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 376 ms to get response, and 384 ms overall 17-03-18 00:10:55.018 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2402648392, 0x0, 559484976) end. Duration was 128 ms. 17-03-18 00:10:55.018 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:55.028 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:10:55.028 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 130 ms. 17-03-18 00:10:55.128 -07 000000482 INF: Autofocus start 17-03-18 00:10:55.128 -07 000000482 INF: MeasureProfile start 17-03-18 00:10:55.128 -07 000000448 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:55.128 -07 000000478 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:55.138 -07 000000448 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:55.138 -07 000000448 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:10:55.138 -07 000000482 INF: MeasureProfile done 17-03-18 00:10:55.138 -07 000000482 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:10:55.138 -07 000000482 INF: Autofocus finished 17-03-18 00:10:55.338 -07 000000382 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:10:55.338 -07 000000448 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:10:55.338 -07 000000610 INF: ------------ Scan Snapping tile 16 at Y-position 72.349609375, Z-position -0.134700738784396. ---------- 17-03-18 00:10:55.338 -07 000000610 INF: Cycle 82 Tile 16 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:10:55.348 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 27933, sync = True, waitForSettling = True) 17-03-18 00:10:55.348 -07 000000610 INF: Z Motor: Moving to -0.127869 mm, started at -0.134701 mm, distance = 6.83 um 17-03-18 00:10:55.348 -07 000000610 INF: FPGA: Xmit ZDACW 27933\n [Z Motor] 17-03-18 00:10:55.348 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:10:55.358 -07 000000610 INF: FPGA: 'ZDACW 27933' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:10:55.358 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:55.358 -07 000000627 INF: FPGA: Recv ZDACR 27933 17-03-18 00:10:55.368 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.368 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:10:55.518 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.518 -07 000000561 INF: FPGA: Recv ZSGSRD 29191 17-03-18 00:10:55.528 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:10:55.528 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.528 -07 000000626 INF: FPGA: Recv ZSGSRD 29196 17-03-18 00:10:55.538 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.538 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.538 -07 000000478 INF: FPGA: Recv ZSGSRD 29194 17-03-18 00:10:55.548 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.548 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.548 -07 000000476 INF: FPGA: Recv ZSGSRD 29195 17-03-18 00:10:55.558 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.558 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.558 -07 000000501 INF: FPGA: Recv ZSGSRD 29195 17-03-18 00:10:55.568 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.568 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.568 -07 000000608 INF: FPGA: Recv ZSGSRD 29199 17-03-18 00:10:55.578 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.578 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.578 -07 000000627 INF: FPGA: Recv ZSGSRD 29191 17-03-18 00:10:55.588 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.588 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.588 -07 000000627 INF: FPGA: Recv ZSGSRD 29191 17-03-18 00:10:55.598 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.598 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.598 -07 000000627 INF: FPGA: Recv ZSGSRD 29194 17-03-18 00:10:55.608 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.608 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:55.608 -07 000000627 INF: FPGA: Recv ZSGSRD 29196 17-03-18 00:10:55.618 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.618 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:55.618 -07 000000627 INF: FPGA: Recv ZPIEZORD 24583 17-03-18 00:10:55.628 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:55.628 -07 000000610 INF: Z Motor: Piezo is near 32.5 V (nominally 0 to 100 V), expect near 42.6 V, since DAC is at 4.262 V. Piezo Error = -10.1 V. 17-03-18 00:10:55.628 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -127.9 um, Read 10 ADC samples: Avg = 29194 (-127.1 uncal um), Error = 0.8 uncal um, Median = 29194 (-127.1 uncal um), StdDev = 3 (0.0 uncal um), Range = 8 (0.1 uncal um), Min = 29191 (-127.1 uncal um), Max = 29199 (-127.1 uncal um), Pos Noise = 5 (0.0 uncal um), Neg Noise = 3 (0.0 uncal um) 17-03-18 00:10:55.628 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29194, posNoise = 5, negNoise = 3 17-03-18 00:10:55.628 -07 000000610 INF: Z Motor: After move to -0.127869, error = -0.000003, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:55.628 -07 000000610 INF: Z Motor: MoveAbsStepped took 279 ms) 17-03-18 00:10:55.628 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:55.628 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:10:55.628 -07 000000627 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:10:55.628 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:10:55.628 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:10:55.628 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:10:55.638 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:10:55.638 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:10:55.638 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:10:55.638 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:10:55.658 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:10:55.658 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:10:55.658 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:10:55.658 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:10:55.658 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 0 ms. 17-03-18 00:10:55.658 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:10:55.658 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 2 ms. 17-03-18 00:10:55.658 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:10:55.668 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 13 ms. 17-03-18 00:10:55.668 -07 000000627 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 17 ms. 17-03-18 00:10:55.678 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28110 150\n [Camera] 17-03-18 00:10:55.878 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:10:56.348 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:10:56.908 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:10:56.908 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:10:56.908 -07 000000569 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:56.908 -07 000000569 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:56.918 -07 000000569 INF: Camera: sBCa_ReadImage(785074288, 2684358704, 0x0, 391868352) start. 17-03-18 00:10:56.918 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:10:56.918 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:10:56.918 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:56.918 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:56.918 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 2719780968, 0x0, 418435064) start. 17-03-18 00:10:57.108 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28110 1422 17-03-18 00:10:57.138 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:10:57.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:10:57.258 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:10:57.318 -07 000000569 INF: Camera: sBCa_ReadImage(785074288, 2684358704, 0x0, 391868352) end. Duration was 401 ms. 17-03-18 00:10:57.318 -07 000000569 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 401 ms. 17-03-18 00:10:57.318 -07 000000569 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:57.318 -07 000000627 INF: Camera: sBCa_ReadImage(785074704, 2719780968, 0x0, 418435064) end. Duration was 400 ms. 17-03-18 00:10:57.318 -07 000000627 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 401 ms. 17-03-18 00:10:57.318 -07 000000627 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:57.318 -07 000000569 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:10:57.318 -07 000000569 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 406 ms. 17-03-18 00:10:57.318 -07 000000627 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:57.328 -07 000000627 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 412 ms. 17-03-18 00:10:57.758 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:10:57.758 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:10:57.758 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:10:57.898 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:10:57.898 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:10:57.898 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:10:57.898 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2755203232, 0x0, 391868352) start. 17-03-18 00:10:57.898 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:10:57.908 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:10:57.908 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 2415923248, 0x0, 418435064) start. 17-03-18 00:10:57.948 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:10:57.948 -07 000000501 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:10:57.948 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28110 150' command took 2 ms to send, 2263 ms to get response, and 2272 ms overall 17-03-18 00:10:57.948 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:10:57.948 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 17 at Y-position 72.349609375, Z-Position -0.127871801277704. ------ 17-03-18 00:10:57.948 -07 000000627 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:10:57.958 -07 000000476 INF: Y Motor: MoveYZ optimized starting. Moving Y to 73.1926 mm, moving Z to -0.13442 mm 17-03-18 00:10:57.958 -07 000000476 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:10:57.958 -07 000000382 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:10:57.958 -07 000000569 INF: Y Motor: Entering MoveAbsStepped (target = 117108 steps, sync = True, waitForSettling = True) 17-03-18 00:10:57.958 -07 000000627 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 2 ms to get response, and 3 ms overall 17-03-18 00:10:57.958 -07 000000569 INF: Y Motor: Moving to 73.1926 mm, started at 72.3496 mm, delta = 0.8430 mm 17-03-18 00:10:57.958 -07 000000569 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:57.958 -07 000000012 INF: Z Motor: Entering MoveAbsStepped (DAC = 29364, sync = True, waitForSettling = False) 17-03-18 00:10:57.958 -07 000000012 INF: Z Motor: Moving to -0.134420 mm, started at -0.127872 mm, distance = -6.55 um 17-03-18 00:10:57.958 -07 000000501 INF: FPGA: Recv YRDPOS 148173 17-03-18 00:10:57.968 -07 000000569 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:10:57.968 -07 000000569 INF: Y Motor: Encoder = 148173 = motor step 115760.2 = 72.3501 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:57.968 -07 000000012 INF: FPGA: Xmit ZDACW 29364\n [Z Motor] 17-03-18 00:10:57.968 -07 000000382 INF: FPGA: Recv ZDACW 17-03-18 00:10:57.968 -07 000000569 INF: Y Motor: Last commanded position = 72.3626 mm (115780.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:10:57.968 -07 000000569 INF: Y Motor: Moving 1348 steps-27 17-03-18 00:10:57.978 -07 000000627 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:10:57.978 -07 000000012 INF: FPGA: 'ZDACW 29364' command took 0 ms to send, 0 ms to get response, and 15 ms overall 17-03-18 00:10:57.988 -07 000000569 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:10:57.998 -07 000000012 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:10:57.998 -07 000000382 INF: FPGA: Recv ZDACR 29364 17-03-18 00:10:58.008 -07 000000012 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 29 ms overall 17-03-18 00:10:58.008 -07 000000012 INF: Z Motor: MoveAbsStepped took 46 ms (did NOT wait for settling yet)) 17-03-18 00:10:58.178 -07 000000012 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:10:58.178 -07 000000627 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 192 ms to get response, and 219 ms overall 17-03-18 00:10:58.288 -07 000000547 INF: Camera: sBCa_ReadImage(785074704, 2415923248, 0x0, 418435064) end. Duration was 383 ms. 17-03-18 00:10:58.288 -07 000000547 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 383 ms. 17-03-18 00:10:58.288 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:58.288 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:58.288 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 2755203232, 0x0, 391868352) end. Duration was 395 ms. 17-03-18 00:10:58.288 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 396 ms. 17-03-18 00:10:58.288 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:10:58.298 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:10:58.298 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 540 ms. 17-03-18 00:10:58.298 -07 000000547 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 401 ms. 17-03-18 00:10:58.368 -07 000000626 INF: FPGA: Recv YMVTILE 17-03-18 00:10:58.368 -07 000000569 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 373 ms to get response, and 395 ms overall 17-03-18 00:10:58.408 -07 000000569 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:58.418 -07 000000547 INF: FPGA: Recv YRDPOS 149864 17-03-18 00:10:58.418 -07 000000569 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:10:58.418 -07 000000569 INF: Y Motor: Encoder = 149864 = motor step 117081.3 = 73.1758 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:58.418 -07 000000569 INF: Y Motor: Position error before settling (table move) = -34 encoder counts (-0.017 mm) 17-03-18 00:10:58.418 -07 000000569 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 395 ms, distance = 0.843 mm, average velocity = 2.134 mm/sec 17-03-18 00:10:59.368 -07 000000569 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:10:59.368 -07 000000512 INF: FPGA: Recv YRDPOS 149864 17-03-18 00:10:59.378 -07 000000569 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.378 -07 000000569 INF: Y Motor: Encoder = 149864 = motor step 117081.3 = 73.1758 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:10:59.398 -07 000000569 INF: Y Motor: After move to 73.192604, error = -0.016823, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:10:59.398 -07 000000569 INF: Y Motor: Position error after settling (table move) = -34 encoder counts (-0.017 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:10:59.398 -07 000000569 INF: Y Motor: Moved to 73.1758 after 1444 ms (table move) 17-03-18 00:10:59.408 -07 000000476 INF: Z Motor: EndOfMoveAbsStepped: target = -0.134420 mm (DAC = 29364) 17-03-18 00:10:59.408 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.408 -07 000000569 INF: FPGA: Recv ZSGSRD 30208 17-03-18 00:10:59.418 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.418 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.418 -07 000000569 INF: FPGA: Recv ZSGSRD 30219 17-03-18 00:10:59.428 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.428 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.428 -07 000000569 INF: FPGA: Recv ZSGSRD 30216 17-03-18 00:10:59.438 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.438 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.438 -07 000000626 INF: FPGA: Recv ZSGSRD 30215 17-03-18 00:10:59.448 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.448 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.448 -07 000000626 INF: FPGA: Recv ZSGSRD 30219 17-03-18 00:10:59.458 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.458 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.458 -07 000000626 INF: FPGA: Recv ZSGSRD 30213 17-03-18 00:10:59.468 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.468 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.468 -07 000000569 INF: FPGA: Recv ZSGSRD 30213 17-03-18 00:10:59.478 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.478 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.478 -07 000000626 INF: FPGA: Recv ZSGSRD 30206 17-03-18 00:10:59.488 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.488 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.488 -07 000000626 INF: FPGA: Recv ZSGSRD 30205 17-03-18 00:10:59.498 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.498 -07 000000476 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:10:59.498 -07 000000626 INF: FPGA: Recv ZSGSRD 30216 17-03-18 00:10:59.508 -07 000000476 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.508 -07 000000476 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:10:59.508 -07 000000569 INF: FPGA: Recv ZPIEZORD 25943 17-03-18 00:10:59.518 -07 000000476 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:10:59.518 -07 000000476 INF: Z Motor: Piezo is near 35.4 V (nominally 0 to 100 V), expect near 44.8 V, since DAC is at 4.481 V. Piezo Error = -9.4 V. 17-03-18 00:10:59.518 -07 000000476 INF: Z Motor: ReadAdcAverage: Commanded Z = -134.4 um, Read 10 ADC samples: Avg = 30213 (-133.6 uncal um), Error = 0.8 uncal um, Median = 30214 (-133.6 uncal um), StdDev = 5 (0.0 uncal um), Range = 14 (0.1 uncal um), Min = 30205 (-133.6 uncal um), Max = 30219 (-133.7 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 8 (0.1 uncal um) 17-03-18 00:10:59.518 -07 000000476 INF: Z Motor: ADC noise = 8, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30213, posNoise = 6, negNoise = 8 17-03-18 00:10:59.518 -07 000000476 INF: Z Motor: After move to -0.134420, error = 0.000002, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:10:59.518 -07 000000476 INF: Z Motor: Settle wait time = 1446 ms, measure time = 110 ms (allowed 150 ms), total time = 1556 ms 17-03-18 00:10:59.518 -07 000000476 INF: Z Motor: Waiting extra 40 ms after reading position to reach expected total of 150 ms 17-03-18 00:10:59.558 -07 000000476 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1600 ms 17-03-18 00:10:59.558 -07 000000476 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:10:59.558 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:10:59.558 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:11:00.148 -07 000000012 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:11:00.148 -07 000000561 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:11:00.148 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:11:00.148 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:11:00.148 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:11:00.148 -07 000000561 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:11:00.148 -07 000000561 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:11:00.148 -07 000000561 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:11:00.168 -07 000000561 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 14 ms. 17-03-18 00:11:00.168 -07 000000561 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 18 ms. 17-03-18 00:11:00.168 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:11:00.208 -07 000000382 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:11:00.508 -07 000000012 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:11:00.508 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:11:00.508 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:11:00.518 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2451345512, 0x0, 559484976) start. 17-03-18 00:11:00.548 -07 000000569 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:11:00.548 -07 000000012 INF: FPGA: Recv CAMSNAP 17-03-18 00:11:00.548 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 377 ms to get response, and 384 ms overall 17-03-18 00:11:00.638 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2451345512, 0x0, 559484976) end. Duration was 126 ms. 17-03-18 00:11:00.638 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:00.638 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:11:00.638 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 129 ms. 17-03-18 00:11:00.748 -07 000000501 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:11:00.748 -07 000000561 INF: Autofocus start 17-03-18 00:11:00.748 -07 000000561 INF: MeasureProfile start 17-03-18 00:11:00.748 -07 000000627 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:11:00.758 -07 000000501 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:11:00.758 -07 000000501 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:11:00.758 -07 000000561 INF: MeasureProfile done 17-03-18 00:11:00.758 -07 000000561 INF: X=16 Z correction [um]=0.0530179 17-03-18 00:11:00.758 -07 000000561 INF: Autofocus finished 17-03-18 00:11:00.958 -07 000000626 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:11:00.958 -07 000000501 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 192 ms to get response, and 202 ms overall 17-03-18 00:11:00.958 -07 000000610 INF: ------------ Scan Snapping tile 17 at Y-position 73.17578125, Z-position -0.134418073450159. ---------- 17-03-18 00:11:00.958 -07 000000610 INF: Cycle 82 Tile 17 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:11:00.968 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 27888, sync = True, waitForSettling = True) 17-03-18 00:11:00.968 -07 000000610 INF: Z Motor: Moving to -0.127663 mm, started at -0.134418 mm, distance = 6.75 um 17-03-18 00:11:00.968 -07 000000610 INF: FPGA: Xmit ZDACW 27888\n [Z Motor] 17-03-18 00:11:00.968 -07 000000547 INF: FPGA: Recv ZDACW 17-03-18 00:11:00.978 -07 000000610 INF: FPGA: 'ZDACW 27888' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:00.978 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:11:00.978 -07 000000486 INF: FPGA: Recv ZDACR 27888 17-03-18 00:11:00.988 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:00.988 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:11:01.138 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.138 -07 000000547 INF: FPGA: Recv ZSGSRD 29159 17-03-18 00:11:01.148 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.148 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.148 -07 000000547 INF: FPGA: Recv ZSGSRD 29164 17-03-18 00:11:01.158 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.158 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.158 -07 000000547 INF: FPGA: Recv ZSGSRD 29166 17-03-18 00:11:01.168 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.168 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.168 -07 000000561 INF: FPGA: Recv ZSGSRD 29159 17-03-18 00:11:01.178 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.178 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.178 -07 000000561 INF: FPGA: Recv ZSGSRD 29164 17-03-18 00:11:01.188 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.188 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.188 -07 000000561 INF: FPGA: Recv ZSGSRD 29163 17-03-18 00:11:01.198 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.198 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.198 -07 000000501 INF: FPGA: Recv ZSGSRD 29169 17-03-18 00:11:01.208 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:11:01.208 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.208 -07 000000561 INF: FPGA: Recv ZSGSRD 29165 17-03-18 00:11:01.218 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.218 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.218 -07 000000501 INF: FPGA: Recv ZSGSRD 29157 17-03-18 00:11:01.228 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.228 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:01.228 -07 000000547 INF: FPGA: Recv ZSGSRD 29165 17-03-18 00:11:01.238 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.238 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:11:01.238 -07 000000012 INF: FPGA: Recv ZPIEZORD 24558 17-03-18 00:11:01.248 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:01.248 -07 000000610 INF: Z Motor: Piezo is near 32.5 V (nominally 0 to 100 V), expect near 42.6 V, since DAC is at 4.255 V. Piezo Error = -10.1 V. 17-03-18 00:11:01.248 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -127.7 um, Read 10 ADC samples: Avg = 29163 (-126.9 uncal um), Error = 0.8 uncal um, Median = 29164 (-126.9 uncal um), StdDev = 4 (0.0 uncal um), Range = 12 (0.1 uncal um), Min = 29157 (-126.9 uncal um), Max = 29169 (-126.9 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 6 (0.0 uncal um) 17-03-18 00:11:01.248 -07 000000610 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29163, posNoise = 6, negNoise = 6 17-03-18 00:11:01.248 -07 000000610 INF: Z Motor: After move to -0.127663, error = -0.000010, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:11:01.248 -07 000000610 INF: Z Motor: MoveAbsStepped took 280 ms) 17-03-18 00:11:01.248 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:11:01.248 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:11:01.248 -07 000000501 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:11:01.248 -07 000000627 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:11:01.248 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:11:01.248 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:11:01.248 -07 000000626 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:11:01.248 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:11:01.248 -07 000000512 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:11:01.248 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:11:01.248 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:11:01.248 -07 000000626 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:11:01.258 -07 000000626 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:11:01.258 -07 000000626 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:11:01.258 -07 000000512 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:11:01.258 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:11:01.268 -07 000000512 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 16 ms. 17-03-18 00:11:01.268 -07 000000512 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 22 ms. 17-03-18 00:11:01.268 -07 000000626 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 16 ms. 17-03-18 00:11:01.268 -07 000000626 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 22 ms. 17-03-18 00:11:01.268 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 28065 150\n [Camera] 17-03-18 00:11:01.468 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:11:01.718 -07 000000547 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:11:02.138 -07 000000486 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:02.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:11:02.508 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:11:02.508 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:11:02.508 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:11:02.508 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:11:02.508 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:11:02.518 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:11:02.518 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:11:02.518 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 2470426896, 0x0, 391868352) start. 17-03-18 00:11:02.518 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:11:02.518 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 2684358704, 0x0, 418435064) start. 17-03-18 00:11:02.698 -07 000000627 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 28065 1422 17-03-18 00:11:02.848 -07 000000012 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:11:02.918 -07 000000561 INF: Camera: sBCa_ReadImage(785074288, 2470426896, 0x0, 391868352) end. Duration was 396 ms. 17-03-18 00:11:02.918 -07 000000561 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 396 ms. 17-03-18 00:11:02.918 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:02.918 -07 000000382 INF: Camera: sBCa_ReadImage(785074704, 2684358704, 0x0, 418435064) end. Duration was 395 ms. 17-03-18 00:11:02.918 -07 000000382 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 396 ms. 17-03-18 00:11:02.918 -07 000000382 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:02.918 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:11:02.918 -07 000000561 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 408 ms. 17-03-18 00:11:02.918 -07 000000382 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:11:02.928 -07 000000382 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 414 ms. 17-03-18 00:11:03.348 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:11:03.348 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:11:03.358 -07 000000569 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:11:03.468 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:11:03.468 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:11:03.468 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:11:03.478 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:11:03.478 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 2415923248, 0x0, 418435064) start. 17-03-18 00:11:03.478 -07 000000569 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:11:03.478 -07 000000569 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:11:03.548 -07 000000382 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:11:03.548 -07 000000547 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:11:03.548 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 28065 150' command took 4 ms to send, 2261 ms to get response, and 2272 ms overall 17-03-18 00:11:03.548 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:11:03.548 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 18 at Y-position 73.17578125, Z-Position -0.12767265070131. ------ 17-03-18 00:11:03.548 -07 000000586 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:11:03.548 -07 000000501 INF: Y Motor: MoveYZ optimized starting. Moving Y to 74.0226 mm, moving Z to -0.13398 mm 17-03-18 00:11:03.548 -07 000000501 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:11:03.558 -07 000000512 INF: Z Motor: Entering MoveAbsStepped (DAC = 29268, sync = True, waitForSettling = False) 17-03-18 00:11:03.558 -07 000000512 INF: Z Motor: Moving to -0.133980 mm, started at -0.127673 mm, distance = -6.31 um 17-03-18 00:11:03.558 -07 000000012 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:11:03.558 -07 000000586 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 1 ms to get response, and 3 ms overall 17-03-18 00:11:03.558 -07 000000512 INF: FPGA: Xmit ZDACW 29268\n [Z Motor] 17-03-18 00:11:03.558 -07 000000627 INF: Y Motor: Entering MoveAbsStepped (target = 118436 steps, sync = True, waitForSettling = True) 17-03-18 00:11:03.558 -07 000000627 INF: Y Motor: Moving to 74.0226 mm, started at 73.1758 mm, delta = 0.8468 mm 17-03-18 00:11:03.558 -07 000000012 INF: FPGA: Recv ZDACW 17-03-18 00:11:03.568 -07 000000512 INF: FPGA: 'ZDACW 29268' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:03.568 -07 000000586 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:11:03.578 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:03.578 -07 000000547 INF: FPGA: Recv YRDPOS 149865 17-03-18 00:11:03.588 -07 000000512 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:11:03.588 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 28 ms overall 17-03-18 00:11:03.588 -07 000000627 INF: Y Motor: Encoder = 149865 = motor step 117082.0 = 73.1763 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:03.588 -07 000000627 INF: Y Motor: Last commanded position = 73.1926 mm (117108.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:11:03.588 -07 000000627 INF: Y Motor: Moving 1354 steps-34 17-03-18 00:11:03.598 -07 000000478 INF: FPGA: Recv ZDACR 29268 17-03-18 00:11:03.598 -07 000000512 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 30 ms overall 17-03-18 00:11:03.598 -07 000000512 INF: Z Motor: MoveAbsStepped took 39 ms (did NOT wait for settling yet)) 17-03-18 00:11:03.598 -07 000000627 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:11:03.768 -07 000000478 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:11:03.768 -07 000000586 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 193 ms to get response, and 211 ms overall 17-03-18 00:11:03.868 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 2415923248, 0x0, 418435064) end. Duration was 391 ms. 17-03-18 00:11:03.868 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 391 ms. 17-03-18 00:11:03.868 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:03.878 -07 000000569 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 395 ms. 17-03-18 00:11:03.878 -07 000000569 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 395 ms. 17-03-18 00:11:03.878 -07 000000569 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:03.878 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:11:03.878 -07 000000569 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:11:03.878 -07 000000569 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 525 ms. 17-03-18 00:11:03.888 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 411 ms. 17-03-18 00:11:03.968 -07 000000586 INF: FPGA: Recv YMVTILE 17-03-18 00:11:03.968 -07 000000627 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 368 ms to get response, and 383 ms overall 17-03-18 00:11:04.008 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:04.008 -07 000000626 INF: FPGA: Recv YRDPOS 151569 17-03-18 00:11:04.018 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:11:04.018 -07 000000627 INF: Y Motor: Encoder = 151569 = motor step 118413.3 = 74.0083 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:04.018 -07 000000627 INF: Y Motor: Position error before settling (table move) = -29 encoder counts (-0.014 mm) 17-03-18 00:11:04.018 -07 000000627 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 383 ms, distance = 0.847 mm, average velocity = 2.211 mm/sec 17-03-18 00:11:04.968 -07 000000627 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:04.968 -07 000000569 INF: FPGA: Recv YRDPOS 151569 17-03-18 00:11:04.978 -07 000000627 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:11:04.978 -07 000000627 INF: Y Motor: Encoder = 151569 = motor step 118413.3 = 74.0083 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:04.998 -07 000000627 INF: Y Motor: After move to 74.022606, error = -0.014305, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:11:04.998 -07 000000627 INF: Y Motor: Position error after settling (table move) = -29 encoder counts (-0.014 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:11:04.998 -07 000000627 INF: Y Motor: Moved to 74.0083 after 1438 ms (table move) 17-03-18 00:11:04.998 -07 000000501 INF: Z Motor: EndOfMoveAbsStepped: target = -0.133980 mm (DAC = 29268) 17-03-18 00:11:04.998 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:04.998 -07 000000627 INF: FPGA: Recv ZSGSRD 30148 17-03-18 00:11:05.008 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:11:05.008 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.008 -07 000000547 INF: FPGA: Recv ZSGSRD 30144 17-03-18 00:11:05.018 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.018 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.018 -07 000000547 INF: FPGA: Recv ZSGSRD 30142 17-03-18 00:11:05.028 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.028 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.028 -07 000000547 INF: FPGA: Recv ZSGSRD 30145 17-03-18 00:11:05.038 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.038 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.038 -07 000000547 INF: FPGA: Recv ZSGSRD 30150 17-03-18 00:11:05.048 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.048 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.048 -07 000000382 INF: FPGA: Recv ZSGSRD 30145 17-03-18 00:11:05.058 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.058 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.058 -07 000000382 INF: FPGA: Recv ZSGSRD 30151 17-03-18 00:11:05.068 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.068 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.068 -07 000000626 INF: FPGA: Recv ZSGSRD 30143 17-03-18 00:11:05.078 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.078 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.078 -07 000000512 INF: FPGA: Recv ZSGSRD 30142 17-03-18 00:11:05.088 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.088 -07 000000501 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:05.088 -07 000000382 INF: FPGA: Recv ZSGSRD 30143 17-03-18 00:11:05.098 -07 000000501 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.098 -07 000000501 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:11:05.098 -07 000000382 INF: FPGA: Recv ZPIEZORD 25911 17-03-18 00:11:05.108 -07 000000501 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.108 -07 000000501 INF: Z Motor: Piezo is near 35.4 V (nominally 0 to 100 V), expect near 44.7 V, since DAC is at 4.466 V. Piezo Error = -9.3 V. 17-03-18 00:11:05.108 -07 000000501 INF: Z Motor: ReadAdcAverage: Commanded Z = -134.0 um, Read 10 ADC samples: Avg = 30145 (-133.2 uncal um), Error = 0.8 uncal um, Median = 30144 (-133.2 uncal um), StdDev = 3 (0.0 uncal um), Range = 9 (0.1 uncal um), Min = 30142 (-133.2 uncal um), Max = 30151 (-133.2 uncal um), Pos Noise = 6 (0.0 uncal um), Neg Noise = 3 (0.0 uncal um) 17-03-18 00:11:05.108 -07 000000501 INF: Z Motor: ADC noise = 6, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30145, posNoise = 6, negNoise = 3 17-03-18 00:11:05.108 -07 000000501 INF: Z Motor: After move to -0.133980, error = -0.000001, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:11:05.108 -07 000000501 INF: Z Motor: Settle wait time = 1442 ms, measure time = 107 ms (allowed 150 ms), total time = 1549 ms 17-03-18 00:11:05.108 -07 000000501 INF: Z Motor: Waiting extra 43 ms after reading position to reach expected total of 150 ms 17-03-18 00:11:05.158 -07 000000501 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1602 ms 17-03-18 00:11:05.158 -07 000000501 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:11:05.158 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:11:05.158 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:11:05.158 -07 000000586 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:11:05.158 -07 000000561 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:11:05.158 -07 000000012 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:11:05.158 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:11:05.158 -07 000000012 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:11:05.158 -07 000000012 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:11:05.168 -07 000000012 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:11:05.168 -07 000000012 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:11:05.178 -07 000000012 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 10 ms. 17-03-18 00:11:05.178 -07 000000012 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 16 ms. 17-03-18 00:11:05.178 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:11:05.218 -07 000000501 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:11:05.518 -07 000000012 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:11:05.518 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:11:05.518 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:11:05.528 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2451345512, 0x0, 559484976) start. 17-03-18 00:11:05.558 -07 000000501 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:11:05.558 -07 000000561 INF: FPGA: Recv CAMSNAP 17-03-18 00:11:05.558 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 379 ms to get response, and 384 ms overall 17-03-18 00:11:05.648 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 2451345512, 0x0, 559484976) end. Duration was 123 ms. 17-03-18 00:11:05.648 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:05.648 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 0 ms. 17-03-18 00:11:05.648 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 126 ms. 17-03-18 00:11:05.658 -07 000000478 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:11:05.658 -07 000000382 INF: Autofocus start 17-03-18 00:11:05.658 -07 000000382 INF: MeasureProfile start 17-03-18 00:11:05.658 -07 000000627 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:11:05.668 -07 000000478 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:11:05.668 -07 000000478 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:11:05.668 -07 000000382 INF: MeasureProfile done 17-03-18 00:11:05.668 -07 000000382 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:11:05.668 -07 000000382 INF: Autofocus finished 17-03-18 00:11:05.868 -07 000000627 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:11:05.868 -07 000000478 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:11:05.868 -07 000000610 INF: ------------ Scan Snapping tile 18 at Y-position 74.00830078125, Z-position -0.133981227024519. ---------- 17-03-18 00:11:05.868 -07 000000610 INF: Cycle 82 Tile 18 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:11:05.878 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 27775, sync = True, waitForSettling = True) 17-03-18 00:11:05.878 -07 000000610 INF: Z Motor: Moving to -0.127146 mm, started at -0.133981 mm, distance = 6.84 um 17-03-18 00:11:05.878 -07 000000610 INF: FPGA: Xmit ZDACW 27775\n [Z Motor] 17-03-18 00:11:05.878 -07 000000501 INF: FPGA: Recv ZDACW 17-03-18 00:11:05.888 -07 000000610 INF: FPGA: 'ZDACW 27775' command took 0 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:11:05.888 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:11:05.888 -07 000000561 INF: FPGA: Recv ZDACR 27775 17-03-18 00:11:05.898 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:05.898 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:11:06.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.048 -07 000000501 INF: FPGA: Recv ZSGSRD 29080 17-03-18 00:11:06.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:11:06.058 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.058 -07 000000561 INF: FPGA: Recv ZSGSRD 29084 17-03-18 00:11:06.068 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.068 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.068 -07 000000501 INF: FPGA: Recv ZSGSRD 29080 17-03-18 00:11:06.078 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.078 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.078 -07 000000486 INF: FPGA: Recv ZSGSRD 29085 17-03-18 00:11:06.088 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.088 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.088 -07 000000501 INF: FPGA: Recv ZSGSRD 29082 17-03-18 00:11:06.098 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.098 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.098 -07 000000569 INF: FPGA: Recv ZSGSRD 29084 17-03-18 00:11:06.108 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.108 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.108 -07 000000561 INF: FPGA: Recv ZSGSRD 29077 17-03-18 00:11:06.118 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.118 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.118 -07 000000501 INF: FPGA: Recv ZSGSRD 29081 17-03-18 00:11:06.128 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.128 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.128 -07 000000561 INF: FPGA: Recv ZSGSRD 29083 17-03-18 00:11:06.138 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.138 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:06.138 -07 000000501 INF: FPGA: Recv ZSGSRD 29085 17-03-18 00:11:06.148 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.148 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:11:06.148 -07 000000486 INF: FPGA: Recv ZPIEZORD 24496 17-03-18 00:11:06.158 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:06.158 -07 000000610 INF: Z Motor: Piezo is near 32.3 V (nominally 0 to 100 V), expect near 42.4 V, since DAC is at 4.238 V. Piezo Error = -10.1 V. 17-03-18 00:11:06.158 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -127.1 um, Read 10 ADC samples: Avg = 29082 (-126.4 uncal um), Error = 0.8 uncal um, Median = 29082 (-126.4 uncal um), StdDev = 3 (0.0 uncal um), Range = 8 (0.1 uncal um), Min = 29077 (-126.3 uncal um), Max = 29085 (-126.4 uncal um), Pos Noise = 3 (0.0 uncal um), Neg Noise = 5 (0.0 uncal um) 17-03-18 00:11:06.158 -07 000000610 INF: Z Motor: ADC noise = 5, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 29082, posNoise = 3, negNoise = 5 17-03-18 00:11:06.158 -07 000000610 INF: Z Motor: After move to -0.127146, error = -0.000006, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:11:06.158 -07 000000610 INF: Z Motor: MoveAbsStepped took 277 ms) 17-03-18 00:11:06.158 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:11:06.158 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:11:06.158 -07 000000382 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:11:06.158 -07 000000547 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:11:06.158 -07 000000626 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:11:06.158 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:11:06.158 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:11:06.158 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:11:06.158 -07 000000626 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:11:06.158 -07 000000626 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:11:06.158 -07 000000627 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:11:06.158 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:11:06.168 -07 000000626 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 2 ms. 17-03-18 00:11:06.168 -07 000000626 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:11:06.168 -07 000000627 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:11:06.168 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:11:06.178 -07 000000626 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:11:06.178 -07 000000626 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 20 ms. 17-03-18 00:11:06.178 -07 000000627 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:11:06.178 -07 000000627 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:11:06.188 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 27952 150\n [Camera] 17-03-18 00:11:06.398 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning red on 199 17-03-18 00:11:07.088 -07 000000586 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:11:07.138 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:07.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:11:07.428 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:11:07.428 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:11:07.428 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:11:07.428 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:11:07.428 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 644204472, 0x0, 391868352) start. 17-03-18 00:11:07.428 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:11:07.428 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:11:07.428 -07 000000501 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:11:07.428 -07 000000501 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:11:07.428 -07 000000501 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) start. 17-03-18 00:11:07.618 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 27952 1422 17-03-18 00:11:07.768 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:11:07.828 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 644204472, 0x0, 391868352) end. Duration was 397 ms. 17-03-18 00:11:07.828 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 398 ms. 17-03-18 00:11:07.828 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:07.828 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:11:07.828 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 401 ms. 17-03-18 00:11:07.828 -07 000000501 INF: Camera: sBCa_ReadImage(785074704, 1441468064, 0x0, 418435064) end. Duration was 398 ms. 17-03-18 00:11:07.828 -07 000000501 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:11:07.828 -07 000000501 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:07.838 -07 000000501 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:11:07.838 -07 000000501 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 409 ms. 17-03-18 00:11:08.268 -07 000000486 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:11:08.268 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:11:08.268 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:11:08.378 -07 000000626 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:11:08.378 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:11:08.388 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:11:08.388 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:11:08.388 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) start. 17-03-18 00:11:08.388 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:11:08.388 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) start. 17-03-18 00:11:08.458 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP 2265 17-03-18 00:11:08.458 -07 000000486 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:11:08.458 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 27952 150' command took 2 ms to send, 2267 ms to get response, and 2277 ms overall 17-03-18 00:11:08.458 -07 000000610 INF: TopSurface Best Z is: -0.172880 - BottomSurface Best Z is: -0.133503 - Using FocusModel for surface 1 - Best Z for this model is: -0.133503 17-03-18 00:11:08.458 -07 000000610 INF: ----- AutoFocus: Snapping image at tile 19 at Y-position 74.00830078125, Z-Position -0.127152289517827. ------ 17-03-18 00:11:08.468 -07 000000501 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:11:08.468 -07 000000586 INF: Y Motor: MoveYZ optimized starting. Moving Y to 74.8526 mm, moving Z to -0.13312 mm 17-03-18 00:11:08.468 -07 000000586 INF: Y Motor: Temporarily changing Y settling times from 3000 or 1100 to 2850 or 950 ms because of parallel Y move 17-03-18 00:11:08.468 -07 000000486 INF: Z Motor: Entering MoveAbsStepped (DAC = 29081, sync = True, waitForSettling = False) 17-03-18 00:11:08.468 -07 000000486 INF: Z Motor: Moving to -0.133124 mm, started at -0.127152 mm, distance = -5.97 um 17-03-18 00:11:08.468 -07 000000547 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:11:08.468 -07 000000626 INF: Y Motor: Entering MoveAbsStepped (target = 119764 steps, sync = True, waitForSettling = True) 17-03-18 00:11:08.468 -07 000000626 INF: Y Motor: Moving to 74.8526 mm, started at 74.0083 mm, delta = 0.8443 mm 17-03-18 00:11:08.478 -07 000000501 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:11:08.478 -07 000000486 INF: FPGA: Xmit ZDACW 29081\n [Z Motor] 17-03-18 00:11:08.478 -07 000000547 INF: FPGA: Recv ZDACW 17-03-18 00:11:08.488 -07 000000486 INF: FPGA: 'ZDACW 29081' command took 0 ms to send, 0 ms to get response, and 13 ms overall 17-03-18 00:11:08.488 -07 000000626 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:08.488 -07 000000547 INF: FPGA: Recv YRDPOS 151570 17-03-18 00:11:08.498 -07 000000626 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 23 ms overall 17-03-18 00:11:08.498 -07 000000626 INF: Y Motor: Encoder = 151570 = motor step 118414.1 = 74.0088 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:08.498 -07 000000501 INF: FPGA: Xmit FOCMRRFOC\n [FocusMirror] 17-03-18 00:11:08.498 -07 000000626 INF: Y Motor: Last commanded position = 74.0226 mm (118436.2 steps), delta from last commanded position = 0.8300 mm (1328.0 steps). UsingTableMove = True 17-03-18 00:11:08.498 -07 000000626 INF: Y Motor: Moving 1350 steps-29 17-03-18 00:11:08.508 -07 000000486 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:11:08.508 -07 000000547 INF: FPGA: Recv ZDACR 29081 17-03-18 00:11:08.518 -07 000000626 INF: FPGA: Xmit YMVTILE 1327\n [Y Motor] 17-03-18 00:11:08.518 -07 000000486 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 30 ms overall 17-03-18 00:11:08.518 -07 000000486 INF: Z Motor: MoveAbsStepped took 44 ms (did NOT wait for settling yet)) 17-03-18 00:11:08.698 -07 000000486 INF: FPGA: Recv FOCMRRFOC 17-03-18 00:11:08.698 -07 000000501 INF: FPGA: 'FOCMRRFOC' command took 0 ms to send, 193 ms to get response, and 222 ms overall 17-03-18 00:11:08.788 -07 000000512 INF: Camera: sBCa_ReadImage(785074288, 1389170736, 0x0, 391868352) end. Duration was 395 ms. 17-03-18 00:11:08.788 -07 000000561 INF: Camera: sBCa_ReadImage(785074704, 639799168, 0x0, 418435064) end. Duration was 398 ms. 17-03-18 00:11:08.788 -07 000000561 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:11:08.788 -07 000000561 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:08.788 -07 000000512 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 395 ms. 17-03-18 00:11:08.788 -07 000000512 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:08.788 -07 000000561 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:11:08.788 -07 000000512 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:11:08.788 -07 000000512 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 518 ms. 17-03-18 00:11:08.798 -07 000000561 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:11:08.898 -07 000000501 INF: FPGA: Recv YMVTILE 17-03-18 00:11:08.898 -07 000000626 INF: FPGA: 'YMVTILE 1327' command took 0 ms to send, 370 ms to get response, and 394 ms overall 17-03-18 00:11:08.918 -07 000000626 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:08.928 -07 000000501 INF: FPGA: Recv YRDPOS 153256 17-03-18 00:11:08.928 -07 000000626 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:11:08.928 -07 000000626 INF: Y Motor: Encoder = 153256 = motor step 119731.3 = 74.8320 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:08.928 -07 000000626 INF: Y Motor: Position error before settling (table move) = -42 encoder counts (-0.021 mm) 17-03-18 00:11:08.928 -07 000000626 INF: Y Motor: FPGA table move command 'YMVTILE 1327' took 394 ms, distance = 0.844 mm, average velocity = 2.143 mm/sec 17-03-18 00:11:09.878 -07 000000626 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:09.878 -07 000000561 INF: FPGA: Recv YRDPOS 153256 17-03-18 00:11:09.888 -07 000000626 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:11:09.888 -07 000000626 INF: Y Motor: Encoder = 153256 = motor step 119731.3 = 74.8320 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:09.898 -07 000000626 INF: Y Motor: After move to 74.852608, error = -0.020576, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:11:09.898 -07 000000626 INF: Y Motor: Position error after settling (table move) = -42 encoder counts (-0.021 mm), Delta during settling = 0 counts (0.000 mm) 17-03-18 00:11:09.898 -07 000000626 INF: Y Motor: Moved to 74.8320 after 1431 ms (table move) 17-03-18 00:11:09.908 -07 000000586 INF: Z Motor: EndOfMoveAbsStepped: target = -0.133124 mm (DAC = 29081) 17-03-18 00:11:09.908 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.908 -07 000000547 INF: FPGA: Recv ZSGSRD 30009 17-03-18 00:11:09.918 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:11:09.918 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.918 -07 000000626 INF: FPGA: Recv ZSGSRD 30010 17-03-18 00:11:09.928 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.928 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.928 -07 000000501 INF: FPGA: Recv ZSGSRD 30010 17-03-18 00:11:09.938 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.938 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.938 -07 000000512 INF: FPGA: Recv ZSGSRD 30014 17-03-18 00:11:09.948 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.948 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.948 -07 000000547 INF: FPGA: Recv ZSGSRD 30003 17-03-18 00:11:09.958 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.958 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.958 -07 000000501 INF: FPGA: Recv ZSGSRD 30018 17-03-18 00:11:09.968 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.968 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.968 -07 000000626 INF: FPGA: Recv ZSGSRD 30017 17-03-18 00:11:09.978 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.978 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.978 -07 000000486 INF: FPGA: Recv ZSGSRD 30014 17-03-18 00:11:09.988 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.988 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.988 -07 000000561 INF: FPGA: Recv ZSGSRD 30018 17-03-18 00:11:09.998 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:09.998 -07 000000586 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:09.998 -07 000000501 INF: FPGA: Recv ZSGSRD 30000 17-03-18 00:11:10.008 -07 000000586 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:10.008 -07 000000586 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:11:10.008 -07 000000486 INF: FPGA: Recv ZPIEZORD 25759 17-03-18 00:11:10.018 -07 000000586 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:10.018 -07 000000586 INF: Z Motor: Piezo is near 35.0 V (nominally 0 to 100 V), expect near 44.4 V, since DAC is at 4.437 V. Piezo Error = -9.3 V. 17-03-18 00:11:10.018 -07 000000586 INF: Z Motor: ReadAdcAverage: Commanded Z = -133.1 um, Read 10 ADC samples: Avg = 30011 (-132.3 uncal um), Error = 0.8 uncal um, Median = 30012 (-132.3 uncal um), StdDev = 6 (0.0 uncal um), Range = 18 (0.1 uncal um), Min = 30000 (-132.3 uncal um), Max = 30018 (-132.4 uncal um), Pos Noise = 7 (0.0 uncal um), Neg Noise = 11 (0.1 uncal um) 17-03-18 00:11:10.018 -07 000000586 INF: Z Motor: ADC noise = 11, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 30011, posNoise = 7, negNoise = 11 17-03-18 00:11:10.018 -07 000000586 INF: Z Motor: After move to -0.133124, error = 0.000004, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:11:10.018 -07 000000586 INF: Z Motor: Settle wait time = 1436 ms, measure time = 108 ms (allowed 150 ms), total time = 1544 ms 17-03-18 00:11:10.018 -07 000000586 INF: Z Motor: Waiting extra 42 ms after reading position to reach expected total of 150 ms 17-03-18 00:11:10.068 -07 000000586 INF: Y Motor: MoveYZ optimized (reads Z position after Y move and settle) took 1596 ms 17-03-18 00:11:10.068 -07 000000586 INF: Y Motor: Restored settling times back to 3000 or 1100 ms 17-03-18 00:11:10.068 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:11:10.068 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:11:10.068 -07 000000561 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:11:10.068 -07 000000512 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:11:10.068 -07 000000501 INF: Disarm and arm camera sensor 0: ROITrigger start. 17-03-18 00:11:10.068 -07 000000501 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:11:10.068 -07 000000501 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:11:10.068 -07 000000501 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) start. 17-03-18 00:11:10.078 -07 000000501 INF: Camera: sBCa_DisarmCaptureImageROITriggered(785074288) end. Duration was 2 ms. 17-03-18 00:11:10.078 -07 000000501 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) start. 17-03-18 00:11:10.078 -07 000000501 INF: Camera: sBCa_ArmCaptureImageROITriggered(785074288, 1024, 512, 255) end. Duration was 6 ms. 17-03-18 00:11:10.078 -07 000000501 INF: Disarm and arm camera sensor 0: ROITrigger end. Duration was 10 ms. 17-03-18 00:11:10.088 -07 000000610 INF: FPGA: Xmit CAMSNAP 1 2 300\n [Camera] 17-03-18 00:11:10.138 -07 000000626 INF: FPGA: Recv @CAMSNAP turning led on 40 17-03-18 00:11:10.438 -07 000000438 INF: FPGA: Recv @CAMSNAP img rdy 340 17-03-18 00:11:10.438 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name Focus T. Args Cmd: img rdy 340 17-03-18 00:11:10.438 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. start. 17-03-18 00:11:10.438 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 675221432, 0x0, 559484976) start. 17-03-18 00:11:10.478 -07 000000626 INF: FPGA: Recv @CAMSNAP 380 17-03-18 00:11:10.478 -07 000000561 INF: FPGA: Recv CAMSNAP 17-03-18 00:11:10.478 -07 000000610 INF: FPGA: 'CAMSNAP 1 2 300' command took 1 ms to send, 383 ms to get response, and 391 ms overall 17-03-18 00:11:10.548 -07 000000005 INF: Camera: sBCa_ReadImageROI(785074288, 1024, 512, 675221432, 0x0, 559484976) end. Duration was 115 ms. 17-03-18 00:11:10.558 -07 000000005 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:10.558 -07 000000005 INF: Camera: CopyActivePixFullImage end. Duration was 1 ms. 17-03-18 00:11:10.558 -07 000000005 INF: Camera 1: ReadROIImage (data transfer) from sensor 0. end. Duration was 117 ms. 17-03-18 00:11:10.578 -07 000000586 INF: FPGA: Xmit FOCMRRCUR 80\n [FocusMirror] 17-03-18 00:11:10.578 -07 000000486 INF: Autofocus start 17-03-18 00:11:10.578 -07 000000486 INF: MeasureProfile start 17-03-18 00:11:10.578 -07 000000502 INF: FPGA: Recv FOCMRRCUR 17-03-18 00:11:10.588 -07 000000586 INF: FPGA: 'FOCMRRCUR 80' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:11:10.588 -07 000000586 INF: FPGA: Xmit FOCMRRIMG\n [FocusMirror] 17-03-18 00:11:10.598 -07 000000486 INF: MeasureProfile done 17-03-18 00:11:10.598 -07 000000486 INF: X=0 Z correction [um]=-0.02394831 17-03-18 00:11:10.598 -07 000000486 INF: Autofocus finished 17-03-18 00:11:10.788 -07 000000501 INF: FPGA: Recv FOCMRRIMG 17-03-18 00:11:10.788 -07 000000586 INF: FPGA: 'FOCMRRIMG' command took 0 ms to send, 193 ms to get response, and 202 ms overall 17-03-18 00:11:10.788 -07 000000610 INF: ------------ Scan Snapping tile 19 at Y-position 74.83203125, Z-position -0.133120382597523. ---------- 17-03-18 00:11:10.798 -07 000000610 INF: Cycle 82 Tile 19 Exposure Settings A 1037 G 500 T 500 C 1037 17-03-18 00:11:10.798 -07 000000610 INF: Z Motor: Entering MoveAbsStepped (DAC = 27588, sync = True, waitForSettling = True) 17-03-18 00:11:10.798 -07 000000610 INF: Z Motor: Moving to -0.126290 mm, started at -0.133120 mm, distance = 6.83 um 17-03-18 00:11:10.798 -07 000000610 INF: FPGA: Xmit ZDACW 27588\n [Z Motor] 17-03-18 00:11:10.798 -07 000000547 INF: FPGA: Recv ZDACW 17-03-18 00:11:10.808 -07 000000610 INF: FPGA: 'ZDACW 27588' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:11:10.808 -07 000000610 INF: FPGA: Xmit ZDACR\n [Z Motor] 17-03-18 00:11:10.808 -07 000000561 INF: FPGA: Recv ZDACR 27588 17-03-18 00:11:10.818 -07 000000610 INF: FPGA: 'ZDACR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:10.818 -07 000000610 INF: Z Motor: Waiting 150 ms for short move 17-03-18 00:11:10.968 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:10.968 -07 000000547 INF: FPGA: Recv ZSGSRD 28949 17-03-18 00:11:10.978 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:11:10.978 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:10.978 -07 000000438 INF: FPGA: Recv ZSGSRD 28949 17-03-18 00:11:10.988 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:10.988 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:10.988 -07 000000512 INF: FPGA: Recv ZSGSRD 28946 17-03-18 00:11:10.998 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:10.998 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:10.998 -07 000000586 INF: FPGA: Recv ZSGSRD 28953 17-03-18 00:11:11.008 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.008 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:11.008 -07 000000512 INF: FPGA: Recv ZSGSRD 28950 17-03-18 00:11:11.018 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.018 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:11.018 -07 000000438 INF: FPGA: Recv ZSGSRD 28951 17-03-18 00:11:11.028 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.028 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:11.028 -07 000000586 INF: FPGA: Recv ZSGSRD 28949 17-03-18 00:11:11.038 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.038 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:11.038 -07 000000438 INF: FPGA: Recv ZSGSRD 28945 17-03-18 00:11:11.048 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.048 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:11.048 -07 000000586 INF: FPGA: Recv ZSGSRD 28952 17-03-18 00:11:11.058 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.058 -07 000000610 INF: FPGA: Xmit ZSGSRD\n [Z Motor] 17-03-18 00:11:11.058 -07 000000438 INF: FPGA: Recv ZSGSRD 28950 17-03-18 00:11:11.068 -07 000000610 INF: FPGA: 'ZSGSRD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.068 -07 000000610 INF: FPGA: Xmit ZPIEZORD\n [Z Motor] 17-03-18 00:11:11.068 -07 000000586 INF: FPGA: Recv ZPIEZORD 24327 17-03-18 00:11:11.078 -07 000000610 INF: FPGA: 'ZPIEZORD' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:11.078 -07 000000610 INF: Z Motor: Piezo is near 32.0 V (nominally 0 to 100 V), expect near 42.1 V, since DAC is at 4.210 V. Piezo Error = -10.1 V. 17-03-18 00:11:11.078 -07 000000610 INF: Z Motor: ReadAdcAverage: Commanded Z = -126.3 um, Read 10 ADC samples: Avg = 28949 (-125.5 uncal um), Error = 0.8 uncal um, Median = 28950 (-125.5 uncal um), StdDev = 2 (0.0 uncal um), Range = 8 (0.1 uncal um), Min = 28945 (-125.5 uncal um), Max = 28953 (-125.6 uncal um), Pos Noise = 4 (0.0 uncal um), Neg Noise = 4 (0.0 uncal um) 17-03-18 00:11:11.078 -07 000000610 INF: Z Motor: ADC noise = 4, warning limit = 655, error limit = 3277, numSamples = 10x8, avg = 28949, posNoise = 4, negNoise = 4 17-03-18 00:11:11.078 -07 000000610 INF: Z Motor: After move to -0.126290, error = -0.000008, min/max error = -0.000831/0.000062, tolerance = 0.004001 mm 17-03-18 00:11:11.078 -07 000000610 INF: Z Motor: MoveAbsStepped took 279 ms) 17-03-18 00:11:11.078 -07 000000610 INF: GreenLED: Using Green data: Target power 180 mW --> 5964.0 mA drive current 17-03-18 00:11:11.078 -07 000000610 INF: RedLED: Using Red data: Target power 205 mW --> 1930.0 mA drive current 17-03-18 00:11:11.078 -07 000000501 INF: RedLED: Enabling LED using existing current: 1930 mA (862 DAC). 17-03-18 00:11:11.078 -07 000000626 INF: GreenLED: Enabling LED using existing current: 5964 mA (2663 DAC). 17-03-18 00:11:11.078 -07 000000561 INF: Disarm and arm camera sensor 0: TwoImagesTrigger start. 17-03-18 00:11:11.078 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) start. 17-03-18 00:11:11.078 -07 000000547 INF: Disarm and arm camera sensor 1: TwoImagesTrigger start. 17-03-18 00:11:11.078 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074704) start. 17-03-18 00:11:11.078 -07 000000561 INF: Camera: sBCa_GetPowerMode(785074288) end. Duration was 1 ms. 17-03-18 00:11:11.078 -07 000000561 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) start. 17-03-18 00:11:11.078 -07 000000547 INF: Camera: sBCa_GetPowerMode(785074704) end. Duration was 1 ms. 17-03-18 00:11:11.078 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) start. 17-03-18 00:11:11.088 -07 000000561 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074288) end. Duration was 3 ms. 17-03-18 00:11:11.088 -07 000000561 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) start. 17-03-18 00:11:11.088 -07 000000547 INF: Camera: sBCa_DisarmCaptureTwoImagesTriggered(785074704) end. Duration was 3 ms. 17-03-18 00:11:11.088 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) start. 17-03-18 00:11:11.098 -07 000000561 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074288, 255) end. Duration was 15 ms. 17-03-18 00:11:11.098 -07 000000561 INF: Disarm and arm camera sensor 0: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:11:11.098 -07 000000547 INF: Camera: sBCa_ArmCaptureTwoImagesTriggered(785074704, 255) end. Duration was 15 ms. 17-03-18 00:11:11.098 -07 000000547 INF: Disarm and arm camera sensor 1: TwoImagesTrigger end. Duration was 21 ms. 17-03-18 00:11:11.098 -07 000000610 INF: FPGA: Xmit CAMDBLSNAP 1037 1037 500 500 27765 150\n [Camera] 17-03-18 00:11:11.298 -07 000000547 INF: FPGA: Recv @CAMDBLSNAP turning red on 192 17-03-18 00:11:12.148 -07 000000586 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:12.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 81. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:11:12.338 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP cam1 red img rdy 1229 17-03-18 00:11:12.338 -07 000000005 INF: Reading out camIdx 0 ledIdx 0 channel image index 3 channel name C. Args Cmd: cam1 red img rdy 1229 17-03-18 00:11:12.338 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:11:12.338 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:11:12.338 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) start. 17-03-18 00:11:12.338 -07 000000438 INF: FPGA: Recv @CAMDBLSNAP cam2 red img rdy 1229 17-03-18 00:11:12.338 -07 000000005 INF: Reading out camIdx 1 ledIdx 0 channel image index 0 channel name A. Args Cmd: cam2 red img rdy 1229 17-03-18 00:11:12.348 -07 000000502 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:11:12.348 -07 000000502 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:11:12.348 -07 000000502 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) start. 17-03-18 00:11:12.458 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:11:12.528 -07 000000586 INF: FPGA: Recv @CAMDBLSNAP Zmove 150 ms 27765 1422 17-03-18 00:11:12.678 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP turning grn on 1572 17-03-18 00:11:12.738 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 1445873368, 0x0, 391868352) end. Duration was 400 ms. 17-03-18 00:11:12.738 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 400 ms. 17-03-18 00:11:12.738 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:12.738 -07 000000502 INF: Camera: sBCa_ReadImage(785074704, 1481295632, 0x0, 418435064) end. Duration was 398 ms. 17-03-18 00:11:12.738 -07 000000502 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 399 ms. 17-03-18 00:11:12.738 -07 000000502 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:12.748 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:11:12.748 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 405 ms. 17-03-18 00:11:12.748 -07 000000502 INF: Camera: CopyActivePixFullImage end. Duration was 4 ms. 17-03-18 00:11:12.758 -07 000000502 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 410 ms. 17-03-18 00:11:13.178 -07 000000561 INF: FPGA: Recv @CAMDBLSNAP cam1 grn img rdy 2072 17-03-18 00:11:13.178 -07 000000005 INF: Reading out camIdx 0 ledIdx 1 channel image index 2 channel name T. Args Cmd: cam1 grn img rdy 2072 17-03-18 00:11:13.178 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. start. 17-03-18 00:11:13.288 -07 000000501 INF: FPGA: Recv @CAMDBLSNAP cam2 grn img rdy 2072 17-03-18 00:11:13.288 -07 000000005 INF: Reading out camIdx 1 ledIdx 1 channel image index 1 channel name G. Args Cmd: cam2 grn img rdy 2072 17-03-18 00:11:13.298 -07 000000586 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. start. 17-03-18 00:11:13.298 -07 000000586 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. start. 17-03-18 00:11:13.298 -07 000000586 INF: Camera: sBCa_ReadImage(785074704, 1389170736, 0x0, 418435064) start. 17-03-18 00:11:13.298 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. start. 17-03-18 00:11:13.298 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) start. 17-03-18 00:11:13.378 -07 000000512 INF: FPGA: Recv @CAMDBLSNAP 2264 17-03-18 00:11:13.378 -07 000000561 INF: FPGA: Recv CAMDBLSNAP 17-03-18 00:11:13.378 -07 000000610 INF: FPGA: 'CAMDBLSNAP 1037 1037 500 500 27765 150' command took 2 ms to send, 2261 ms to get response, and 2272 ms overall 17-03-18 00:11:13.688 -07 000000586 INF: Camera: sBCa_ReadImage(785074704, 1389170736, 0x0, 418435064) end. Duration was 391 ms. 17-03-18 00:11:13.688 -07 000000586 INF: Camera 2: ReadSquareImageHardwareTrigger (data transfer) from sensor 1. end. Duration was 391 ms. 17-03-18 00:11:13.688 -07 000000586 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:13.698 -07 000000586 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:11:13.698 -07 000000547 INF: Camera: sBCa_ReadImage(785074288, 639799168, 0x0, 391868352) end. Duration was 396 ms. 17-03-18 00:11:13.698 -07 000000547 INF: Camera 1: ReadSquareImageHardwareTrigger (data transfer) from sensor 0. end. Duration was 396 ms. 17-03-18 00:11:13.698 -07 000000547 INF: Camera: CopyActivePixFullImage start. 17-03-18 00:11:13.698 -07 000000586 INF: Camera 2: ReadSquareImage (data transfer) from sensor 1. end. Duration was 406 ms. 17-03-18 00:11:13.698 -07 000000547 INF: Camera: CopyActivePixFullImage end. Duration was 3 ms. 17-03-18 00:11:13.698 -07 000000547 INF: Camera 1: ReadSquareImage (data transfer) from sensor 0. end. Duration was 519 ms. 17-03-18 00:11:13.698 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074288, 0) start. 17-03-18 00:11:13.748 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074288, 0) end. Duration was 42 ms. 17-03-18 00:11:13.748 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074704, 0) start. 17-03-18 00:11:13.788 -07 000000610 INF: Camera: sBCa_SetPowerMode(785074704, 0) end. Duration was 42 ms. 17-03-18 00:11:13.798 -07 000000610 INF: Enabling Skipping AutoFocus in the next 5 cycles. 17-03-18 00:11:13.798 -07 000000610 INF: Imaging Cycle 82: ScanTilesCommand Cycle 82 time end. Duration was 196708 ms. 17-03-18 00:11:13.798 -07 000000610 INF: =================== Cycle #82 =================== 17-03-18 00:11:13.798 -07 000000610 INF: =================== =================== 17-03-18 00:11:13.808 -07 000000561 INF: FPGA: Xmit EXLED1ADCR\n [GreenLED] 17-03-18 00:11:13.808 -07 000000586 INF: FPGA: Recv EXLED1ADCR 20 17-03-18 00:11:13.808 -07 000000610 INF: Y Motor: Entering MoveAbsStepped (target = 120609 steps, sync = True, waitForSettling = True) 17-03-18 00:11:13.808 -07 000000610 INF: Y Motor: Moving to 75.3806 mm, started at 74.8320 mm, delta = 0.5485 mm 17-03-18 00:11:13.818 -07 000000561 INF: FPGA: 'EXLED1ADCR' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:11:13.818 -07 000000561 INF: GreenLED: Using Green data: Photodiode ADC 20 --> 0.0 mW (old method), or 1.4 mW (new method). Difference = 1.4 mW 17-03-18 00:11:13.818 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:13.818 -07 000000561 INF: Predictive Diagnostic Log Cycle Entry Metrics: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, FrontChillerHeatSinkTemperature: 0.469694, AmbientTemperature: 18.5769, FlowCellCompartmentTemperature: 21.99836, FlowCellTargetTemperature: 22, FlowCellHeatSinkTemperature: 18.16216, GreenLEDSensorTemperature: 29.88599, PhotodiodePowerAdcGreen: 20, PhotodiodePowerMwGreen: 1.379937 17-03-18 00:11:13.818 -07 000000626 INF: FPGA: Recv YRDPOS 153256 17-03-18 00:11:13.828 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 15 ms overall 17-03-18 00:11:13.828 -07 000000610 INF: Y Motor: Encoder = 153256 = motor step 119731.3 = 74.8320 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:13.828 -07 000000610 INF: Y Motor: Last commanded position = 74.8526 mm (119764.2 steps), delta from last commanded position = 0.5280 mm (844.8 steps). UsingTableMove = False 17-03-18 00:11:13.828 -07 000000610 INF: Y Motor: Moving to 154379 encoder counts 17-03-18 00:11:13.828 -07 000000610 INF: FPGA: Xmit YMVTO 154379\n [Y Motor] 17-03-18 00:11:13.938 -07 000000501 INF: FPGA: Recv YMVTO 154379 878 17-03-18 00:11:13.938 -07 000000610 INF: FPGA: 'YMVTO 154379' command took 0 ms to send, 108 ms to get response, and 117 ms overall 17-03-18 00:11:13.938 -07 000000610 INF: Y Motor: Position error before settling = 0 encoder counts (0.000 mm) 17-03-18 00:11:13.938 -07 000000610 INF: Y Motor: To move from 74.832 to 75.381 (0.549 mm) should take 877 steps, but required 878 steps (0.549 mm). Slippage = 0.001 mm (0.00%). 17-03-18 00:11:13.938 -07 000000610 INF: Y Motor: FPGA move command 'YMVTO 154379' took 117 ms, distance = 0.549 mm, average velocity = 4.688 mm/sec 17-03-18 00:11:16.938 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:16.938 -07 000000512 INF: FPGA: Recv YRDPOS 154387 17-03-18 00:11:16.948 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:11:16.948 -07 000000610 INF: Y Motor: Encoder = 154387 = motor step 120614.8 = 75.3843 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:16.968 -07 000000610 INF: Y Motor: After move to 75.380580, error = 0.003697, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:11:16.968 -07 000000610 INF: Y Motor: Position error after settling = 8 encoder counts (0.004 mm), Delta during settling = 8 counts (0.004 mm) 17-03-18 00:11:16.968 -07 000000610 INF: Y Motor: Moved to 75.3843 after 3156 ms 17-03-18 00:11:16.968 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:11:16.968 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:11:16.988 -07 000000610 INF: ReagentValve: Recv 18\r 17-03-18 00:11:16.988 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 24 to 3. Moving 3 positions. 17-03-18 00:11:16.988 -07 000000610 INF: ReagentValve: Xmit +03\r 17-03-18 00:11:17.138 -07 000000547 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:17.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1309, R2=1360, Total=2669. Warn if over 250 bubbles per interval. 17-03-18 00:11:17.288 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:11:17.308 -07 000000610 INF: ReagentValve: Recv 0278\r 17-03-18 00:11:17.308 -07 000000610 INF: ReagentValve: Move+ from 24 to 3 took 632 steps, expected 612. Error = 20 steps (3.3%) 17-03-18 00:11:17.358 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:11:17.378 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:11:17.378 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:11:17.478 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:17.478 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:11:17.478 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:17.508 -07 000000610 INF: LanePump: Recv /0`0(0x03)\r\n 17-03-18 00:11:17.508 -07 000000610 INF: LanePump: Current Position of Pump is: 0 17-03-18 00:11:17.508 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:11:17.638 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:11:17.678 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:17.828 -07 000000561 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:11:20.808 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:20.838 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:21.258 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:21.288 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:21.708 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:21.738 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:21.738 -07 000000610 INF: LanePump: Waited 4059 ms for pump motion to complete 17-03-18 00:11:21.738 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:21.868 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:21.898 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:11:21.898 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:11:22.028 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:22.058 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:11:22.058 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:11:22.058 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:11:22.138 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 5 R1 6 17-03-18 00:11:22.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=6, R2=5, Total=11. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1315, R2=1365, Total=2680. Warn if over 250 bubbles per interval. 17-03-18 00:11:22.188 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:11:22.218 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:22.648 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:22.678 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:22.678 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:11:22.678 -07 000000610 INF: =================== =================== 17-03-18 00:11:22.718 -07 000000610 INF: Y Motor: Entering MoveAbsStepped (target = 120609 steps, sync = True, waitForSettling = True) 17-03-18 00:11:22.718 -07 000000610 INF: Y Motor: Moving to 75.3806 mm, started at 75.3843 mm, delta = -0.0037 mm 17-03-18 00:11:22.718 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:22.728 -07 000000561 INF: FPGA: Recv YRDPOS 154387 17-03-18 00:11:22.728 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 3 ms overall 17-03-18 00:11:22.728 -07 000000610 INF: Y Motor: Encoder = 154387 = motor step 120614.8 = 75.3843 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:22.728 -07 000000610 INF: Y Motor: Last commanded position = 75.3806 mm (120608.9 steps), delta from last commanded position = 0.0000 mm (0.0 steps). UsingTableMove = False 17-03-18 00:11:22.728 -07 000000610 INF: Y Motor: Moving to 154379 encoder counts 17-03-18 00:11:22.728 -07 000000610 INF: FPGA: Xmit YMVTO 154379\n [Y Motor] 17-03-18 00:11:22.758 -07 000000561 INF: FPGA: Recv YMVTO 154379 12 17-03-18 00:11:22.758 -07 000000610 INF: FPGA: 'YMVTO 154379' command took 0 ms to send, 28 ms to get response, and 37 ms overall 17-03-18 00:11:22.758 -07 000000610 INF: Y Motor: Position error before settling = 0 encoder counts (0.000 mm) 17-03-18 00:11:22.758 -07 000000610 INF: Y Motor: To move from 75.384 to 75.381 (-0.004 mm) should take 6 steps, but required 12 steps (0.008 mm). Slippage = 0.004 mm (-1.01%). 17-03-18 00:11:22.758 -07 000000610 INF: Y Motor: FPGA move command 'YMVTO 154379' took 38 ms, distance = 0.004 mm, average velocity = 0.097 mm/sec 17-03-18 00:11:23.198 -07 000000012 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:11:25.758 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:25.758 -07 000000627 INF: FPGA: Recv YRDPOS 154373 17-03-18 00:11:25.768 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:11:25.768 -07 000000610 INF: Y Motor: Encoder = 154373 = motor step 120603.9 = 75.3774 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:25.768 -07 000000610 INF: Y Motor: After move to 75.380580, error = -0.003139, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:11:25.768 -07 000000610 INF: Y Motor: Position error after settling = -6 encoder counts (-0.003 mm), Delta during settling = -6 counts (-0.003 mm) 17-03-18 00:11:25.768 -07 000000610 INF: Y Motor: Moved to 75.3774 after 3046 ms 17-03-18 00:11:25.778 -07 000000610 INF: =================== Flow Cell Temp 60° =================== 17-03-18 00:11:25.778 -07 000000610 INF: =================== Flow Cell Temp 60°, FC temperature to 60° =================== 17-03-18 00:11:25.778 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 60000000\n [FlowCellTempControl] 17-03-18 00:11:25.788 -07 000000512 INF: FPGA: Recv TEC2TEMP 17-03-18 00:11:25.788 -07 000000610 INF: FPGA: 'TEC2TEMP 0 60000000' command took 1 ms to send, 1 ms to get response, and 3 ms overall 17-03-18 00:11:25.788 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:11:25.788 -07 000000012 INF: FPGA: Recv TEC2EN 17-03-18 00:11:25.798 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:11:25.798 -07 000000610 INF: =================== Flow Cell Temp 60° =================== 17-03-18 00:11:25.798 -07 000000610 INF: =================== =================== 17-03-18 00:11:25.798 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:25.798 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:11:25.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:25.838 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:11:25.838 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:11:25.838 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:11:25.968 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:11:26.008 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:27.138 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:27.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1315, R2=1365, Total=2680. Warn if over 250 bubbles per interval. 17-03-18 00:11:29.138 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:29.218 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:11:29.228 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:29.648 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:29.678 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:30.098 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:30.128 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:30.128 -07 000000610 INF: LanePump: Waited 4119 ms for pump motion to complete 17-03-18 00:11:30.128 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:30.258 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:30.288 -07 000000610 INF: LanePump: Recv /0`1440(0x03)\r\n 17-03-18 00:11:30.288 -07 000000610 INF: LanePump: Current Position of Pump is: 1440 17-03-18 00:11:30.418 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:30.448 -07 000000610 INF: LanePump: Recv /0`1440(0x03)\r\n 17-03-18 00:11:30.448 -07 000000610 INF: LanePump: Current Position of Pump is: 1440 17-03-18 00:11:30.448 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:11:30.578 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:11:30.608 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:31.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:31.068 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:31.068 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:11:31.068 -07 000000610 INF: =================== =================== 17-03-18 00:11:32.138 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 2 R1 2 17-03-18 00:11:32.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=2, R2=2, Total=4. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1317, R2=1367, Total=2684. Warn if over 250 bubbles per interval. 17-03-18 00:11:32.288 -07 000000610 INF: AsyncDelayTimer waited 1217ms 17-03-18 00:11:32.288 -07 000000610 INF: ReagentValve: Go to position 4 - PumpToFlowcell start 17-03-18 00:11:32.288 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:11:32.308 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:11:32.308 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 3 to 4. Moving 1 positions. 17-03-18 00:11:32.308 -07 000000610 INF: ReagentValve: Xmit +04\r 17-03-18 00:11:32.438 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:11:32.458 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:11:32.458 -07 000000610 INF: ReagentValve: Move+ from 3 to 4 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:11:32.508 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:11:32.528 -07 000000610 INF: ReagentValve: Recv 04\r 17-03-18 00:11:32.528 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:11:32.628 -07 000000610 INF: =================== Pumping Reagent CMS (4) =================== 17-03-18 00:11:32.628 -07 000000610 INF: LanePump: Pumping from 4, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 45 µL, calling command Pumping to Flow Cell #1 17-03-18 00:11:32.628 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:32.658 -07 000000610 INF: LanePump: Recv /0`1440(0x03)\r\n 17-03-18 00:11:32.658 -07 000000610 INF: LanePump: Current Position of Pump is: 1440 17-03-18 00:11:32.658 -07 000000610 INF: =================== Pumping Reagent CMS (4), Aspirating port InputPort =================== 17-03-18 00:11:32.788 -07 000000610 INF: LanePump: Xmit /1V400IP270R\r 17-03-18 00:11:32.828 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:34.268 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:34.298 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:34.718 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:34.748 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:34.748 -07 000000610 INF: LanePump: Waited 1919 ms for pump motion to complete 17-03-18 00:11:34.748 -07 000000610 INF: =================== Pumping Reagent CMS (4) =================== 17-03-18 00:11:34.858 -07 000000502 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:11:34.878 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:34.908 -07 000000610 INF: LanePump: Recv /0`1710(0x03)\r\n 17-03-18 00:11:34.908 -07 000000610 INF: LanePump: Current Position of Pump is: 1710 17-03-18 00:11:35.038 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:35.068 -07 000000610 INF: LanePump: Recv /0`1710(0x03)\r\n 17-03-18 00:11:35.068 -07 000000610 INF: LanePump: Current Position of Pump is: 1710 17-03-18 00:11:35.068 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:11:35.198 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:11:35.228 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:35.658 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:35.688 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:35.688 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:11:35.688 -07 000000610 INF: =================== =================== 17-03-18 00:11:36.908 -07 000000610 INF: AsyncDelayTimer waited 1213ms 17-03-18 00:11:36.908 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:11:36.908 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:11:36.928 -07 000000610 INF: ReagentValve: Recv 04\r 17-03-18 00:11:36.928 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 4 to 3. Moving 1 positions. 17-03-18 00:11:36.928 -07 000000610 INF: ReagentValve: Xmit -03\r 17-03-18 00:11:37.078 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:11:37.098 -07 000000610 INF: ReagentValve: Recv 00E1\r 17-03-18 00:11:37.098 -07 000000610 INF: ReagentValve: Move- from 4 to 3 took 225 steps, expected 204. Error = 21 steps (10.3%) 17-03-18 00:11:37.138 -07 000000478 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:37.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1317, R2=1367, Total=2684. Warn if over 250 bubbles per interval. 17-03-18 00:11:37.148 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:11:37.168 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:11:37.168 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:11:37.268 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:37.268 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 20 µL, calling command Pumping to Flow Cell #1 17-03-18 00:11:37.268 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:37.308 -07 000000610 INF: LanePump: Recv /0`1710(0x03)\r\n 17-03-18 00:11:37.308 -07 000000610 INF: LanePump: Current Position of Pump is: 1710 17-03-18 00:11:37.308 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:11:37.438 -07 000000610 INF: LanePump: Xmit /1V400IP120R\r 17-03-18 00:11:37.478 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:38.348 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:38.378 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:38.378 -07 000000610 INF: LanePump: Waited 900 ms for pump motion to complete 17-03-18 00:11:38.378 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:38.508 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:38.538 -07 000000610 INF: LanePump: Recv /0`1830(0x03)\r\n 17-03-18 00:11:38.538 -07 000000610 INF: LanePump: Current Position of Pump is: 1830 17-03-18 00:11:38.668 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:38.698 -07 000000610 INF: LanePump: Recv /0`1830(0x03)\r\n 17-03-18 00:11:38.698 -07 000000610 INF: LanePump: Current Position of Pump is: 1830 17-03-18 00:11:38.698 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:11:38.828 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:11:38.858 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:39.288 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:39.318 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:39.318 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:11:39.318 -07 000000610 INF: =================== =================== 17-03-18 00:11:39.338 -07 000000626 INF: WaitCommand(10000) start 17-03-18 00:11:39.338 -07 000000012 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:11:39.338 -07 000000012 INF: =================== Dispense to DispenseAndWait, Dispensing port OutputPort =================== 17-03-18 00:11:40.228 -07 000000486 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:11:40.538 -07 000000012 INF: AsyncDelayTimer waited 1199ms 17-03-18 00:11:40.538 -07 000000012 INF: LanePump: Xmit /1?\r 17-03-18 00:11:41.148 -07 000000012 INF: LanePump: Recv /0`1830(0x03)\r\n 17-03-18 00:11:41.148 -07 000000012 INF: LanePump: Current Position of Pump is: 1830 17-03-18 00:11:41.278 -07 000000012 INF: LanePump: Xmit /1V1500OA0R\r 17-03-18 00:11:41.318 -07 000000012 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:42.138 -07 000000486 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:42.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1317, R2=1367, Total=2684. Warn if over 250 bubbles per interval. 17-03-18 00:11:43.568 -07 000000012 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:43.598 -07 000000012 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:44.018 -07 000000012 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:44.048 -07 000000012 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:44.048 -07 000000012 INF: LanePump: Waited 2729 ms for pump motion to complete 17-03-18 00:11:44.048 -07 000000012 INF: =================== Dispense to DispenseAndWait =================== 17-03-18 00:11:44.048 -07 000000012 INF: =================== =================== 17-03-18 00:11:46.458 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:11:47.138 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:47.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1317, R2=1367, Total=2684. Warn if over 250 bubbles per interval. 17-03-18 00:11:49.348 -07 000000626 INF: WaitCommand(10000) end 17-03-18 00:11:49.348 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:49.348 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 250 µL, calling command Pumping to Flow Cell #1 17-03-18 00:11:49.348 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:49.388 -07 000000610 INF: LanePump: Recv /0`0(0x03)\r\n 17-03-18 00:11:49.388 -07 000000610 INF: LanePump: Current Position of Pump is: 0 17-03-18 00:11:49.388 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:11:49.518 -07 000000610 INF: LanePump: Xmit /1V400IP1500R\r 17-03-18 00:11:49.558 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:51.818 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:11:52.138 -07 000000626 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:11:52.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1317, R2=1367, Total=2684. Warn if over 250 bubbles per interval. 17-03-18 00:11:55.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:55.718 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:56.138 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:56.168 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:56.588 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:56.618 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:57.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:57.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:11:57.138 -07 000000561 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 4 R1 3 17-03-18 00:11:57.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=3, R2=4, Total=7. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:11:57.178 -07 000000502 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:11:57.488 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:58.028 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:58.028 -07 000000610 INF: LanePump: Waited 8469 ms for pump motion to complete 17-03-18 00:11:58.028 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:11:58.158 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:58.188 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:11:58.188 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:11:58.318 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:11:58.348 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:11:58.348 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:11:58.348 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:11:58.478 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:11:58.508 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:58.938 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:11:58.968 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:11:58.968 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:11:58.968 -07 000000610 INF: =================== =================== 17-03-18 00:11:59.008 -07 000000610 INF: Y Motor: Entering MoveAbsStepped (target = 120609 steps, sync = True, waitForSettling = True) 17-03-18 00:11:59.008 -07 000000610 INF: Y Motor: Moving to 75.3806 mm, started at 75.3774 mm, delta = 0.0031 mm 17-03-18 00:11:59.008 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:11:59.008 -07 000000501 INF: FPGA: Recv YRDPOS 154373 17-03-18 00:11:59.018 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 4 ms overall 17-03-18 00:11:59.018 -07 000000610 INF: Y Motor: Encoder = 154373 = motor step 120603.9 = 75.3774 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:11:59.018 -07 000000610 INF: Y Motor: Last commanded position = 75.3806 mm (120608.9 steps), delta from last commanded position = 0.0000 mm (0.0 steps). UsingTableMove = False 17-03-18 00:11:59.018 -07 000000610 INF: Y Motor: Moving to 154379 encoder counts 17-03-18 00:11:59.018 -07 000000610 INF: FPGA: Xmit YMVTO 154379\n [Y Motor] 17-03-18 00:11:59.048 -07 000000501 INF: FPGA: Recv YMVTO 154379 10 17-03-18 00:11:59.048 -07 000000610 INF: FPGA: 'YMVTO 154379' command took 0 ms to send, 28 ms to get response, and 37 ms overall 17-03-18 00:11:59.048 -07 000000610 INF: Y Motor: Position error before settling = 0 encoder counts (0.000 mm) 17-03-18 00:11:59.048 -07 000000610 INF: Y Motor: To move from 75.377 to 75.381 (0.003 mm) should take 5 steps, but required 10 steps (0.006 mm). Slippage = 0.003 mm (1.00%). 17-03-18 00:11:59.048 -07 000000610 INF: Y Motor: FPGA move command 'YMVTO 154379' took 37 ms, distance = 0.003 mm, average velocity = 0.085 mm/sec 17-03-18 00:12:02.048 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:12:02.048 -07 000000626 INF: FPGA: Recv YRDPOS 154385 17-03-18 00:12:02.058 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 10 ms overall 17-03-18 00:12:02.058 -07 000000610 INF: Y Motor: Encoder = 154385 = motor step 120613.3 = 75.3833 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:12:02.058 -07 000000610 INF: Y Motor: After move to 75.380580, error = 0.002721, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:12:02.058 -07 000000610 INF: Y Motor: Position error after settling = 6 encoder counts (0.003 mm), Delta during settling = 6 counts (0.003 mm) 17-03-18 00:12:02.058 -07 000000610 INF: Y Motor: Moved to 75.3833 after 3048 ms 17-03-18 00:12:02.058 -07 000000610 INF: =================== Flow Cell Temp 20° =================== 17-03-18 00:12:02.068 -07 000000610 INF: =================== Flow Cell Temp 20°, FC temperature to 20° =================== 17-03-18 00:12:02.068 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 20000000\n [FlowCellTempControl] 17-03-18 00:12:02.068 -07 000000382 INF: FPGA: Recv TEC2TEMP 17-03-18 00:12:02.078 -07 000000610 INF: FPGA: 'TEC2TEMP 0 20000000' command took 1 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:12:02.078 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:12:02.078 -07 000000626 INF: FPGA: Recv TEC2EN 17-03-18 00:12:02.088 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:12:02.088 -07 000000610 INF: =================== Flow Cell Temp 20° =================== 17-03-18 00:12:02.098 -07 000000610 INF: =================== =================== 17-03-18 00:12:02.098 -07 000000610 INF: WaitCommand(120000) start 17-03-18 00:12:02.138 -07 000000626 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:02.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:02.538 -07 000000012 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:12:07.138 -07 000000438 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:07.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:07.898 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:12:12.138 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:12.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:13.258 -07 000000012 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.018051, 17-03-18 00:12:17.138 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:17.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:18.618 -07 000000626 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.03576, 17-03-18 00:12:22.138 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:22.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:24.688 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:12:27.138 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:27.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:30.048 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:12:32.138 -07 000000012 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:32.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:35.408 -07 000000012 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:12:37.138 -07 000000502 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:37.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:40.768 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:12:42.138 -07 000000012 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:42.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:46.128 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:12:47.138 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:47.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:51.488 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:12:52.138 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:52.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:12:56.848 -07 000000502 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:12:57.138 -07 000000502 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:12:57.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:02.138 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:02.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:02.208 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:13:07.138 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:07.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:07.568 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:13:12.138 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:12.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:12.928 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.03576, 17-03-18 00:13:17.218 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:17.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:18.288 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:13:22.138 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:22.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:23.648 -07 000000438 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:13:27.138 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:27.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:29.008 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:13:32.138 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:32.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:34.368 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.970374, 17-03-18 00:13:37.138 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:37.138 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:39.728 -07 000000012 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:13:42.138 -07 000000438 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:42.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:45.088 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.028963, 17-03-18 00:13:47.138 -07 000000438 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:47.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:50.448 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:13:52.138 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:52.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:13:55.808 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:13:57.148 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:13:57.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:14:01.168 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:14:02.118 -07 000000610 INF: WaitCommand(120000) end 17-03-18 00:14:02.118 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:14:02.118 -07 000000610 INF: ReagentValve: Go to position 11 - PumpToFlowcell start 17-03-18 00:14:02.118 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:14:02.138 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:14:02.138 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 3 to 11. Moving 8 positions. 17-03-18 00:14:02.138 -07 000000610 INF: ReagentValve: Xmit +0B\r 17-03-18 00:14:02.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:02.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:14:02.828 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:14:02.848 -07 000000610 INF: ReagentValve: Recv 0675\r 17-03-18 00:14:02.848 -07 000000610 INF: ReagentValve: Move+ from 3 to 11 took 1653 steps, expected 1633. Error = 20 steps (1.2%) 17-03-18 00:14:02.898 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:14:02.918 -07 000000610 INF: ReagentValve: Recv 0B\r 17-03-18 00:14:02.918 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:14:03.018 -07 000000610 INF: =================== Pumping Reagent RMF (11) =================== 17-03-18 00:14:03.018 -07 000000610 INF: LanePump: Pumping from 11, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 300 µL, calling command Pumping to Flow Cell #1 17-03-18 00:14:03.098 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:03.128 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:14:03.128 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:14:03.258 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:03.288 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:14:03.288 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:14:06.528 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:14:07.148 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:07.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:14:07.458 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:07.488 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:07.488 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:14:07.618 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:14:07.648 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:08.078 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:08.108 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:08.108 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:14:08.238 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:08.268 -07 000000610 INF: LanePump: Recv /0`1500(0x03)\r\n 17-03-18 00:14:08.268 -07 000000610 INF: LanePump: Current Position of Pump is: 1500 17-03-18 00:14:08.398 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:14:08.438 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:11.888 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:14:12.148 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:12.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:14:13.358 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:13.388 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:13.808 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:13.838 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:14.258 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:14.288 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:14.708 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:14.738 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:14.738 -07 000000610 INF: LanePump: Waited 6299 ms for pump motion to complete 17-03-18 00:14:14.738 -07 000000610 INF: =================== Pumping Reagent RMF (11), Aspirating port InputPort =================== 17-03-18 00:14:14.868 -07 000000610 INF: LanePump: Xmit /1V400IP1800R\r 17-03-18 00:14:14.908 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:17.148 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:17.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1320, R2=1371, Total=2691. Warn if over 250 bubbles per interval. 17-03-18 00:14:17.248 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.956714, 17-03-18 00:14:22.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 5 R1 6 17-03-18 00:14:22.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=6, R2=5, Total=11. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1326, R2=1376, Total=2702. Warn if over 250 bubbles per interval. 17-03-18 00:14:22.328 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:22.358 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:22.608 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:14:22.778 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:22.808 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:23.228 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:23.258 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:23.678 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:23.708 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:24.128 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:24.158 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:24.578 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:24.608 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:24.608 -07 000000610 INF: LanePump: Waited 9699 ms for pump motion to complete 17-03-18 00:14:24.608 -07 000000610 INF: =================== Pumping Reagent RMF (11) =================== 17-03-18 00:14:24.738 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:24.768 -07 000000610 INF: LanePump: Recv /0`1800(0x03)\r\n 17-03-18 00:14:24.768 -07 000000610 INF: LanePump: Current Position of Pump is: 1800 17-03-18 00:14:24.898 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:24.928 -07 000000610 INF: LanePump: Recv /0`1800(0x03)\r\n 17-03-18 00:14:24.928 -07 000000610 INF: LanePump: Current Position of Pump is: 1800 17-03-18 00:14:24.928 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:14:25.058 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:14:25.088 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:25.518 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:25.548 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:25.548 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:14:25.548 -07 000000610 INF: =================== =================== 17-03-18 00:14:25.548 -07 000000610 INF: =================== Pumping Reagent RMF (11) =================== 17-03-18 00:14:25.548 -07 000000610 INF: LanePump: Pumping from 11, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 75 µL, calling command Pumping to Flow Cell #1 17-03-18 00:14:25.678 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:25.708 -07 000000610 INF: LanePump: Recv /0`1800(0x03)\r\n 17-03-18 00:14:25.708 -07 000000610 INF: LanePump: Current Position of Pump is: 1800 17-03-18 00:14:25.708 -07 000000610 INF: =================== Pumping Reagent RMF (11), Aspirating port InputPort =================== 17-03-18 00:14:25.838 -07 000000610 INF: LanePump: Xmit /1V50IP450R\r 17-03-18 00:14:25.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:27.148 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 13 R1 12 17-03-18 00:14:27.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=12, R2=13, Total=25. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1389, Total=2727. Warn if over 250 bubbles per interval. 17-03-18 00:14:27.968 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:14:32.148 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:32.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1389, Total=2727. Warn if over 250 bubbles per interval. 17-03-18 00:14:33.328 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:14:37.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:37.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1389, Total=2727. Warn if over 250 bubbles per interval. 17-03-18 00:14:38.688 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.052111, 17-03-18 00:14:42.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:42.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1389, Total=2727. Warn if over 250 bubbles per interval. 17-03-18 00:14:42.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:42.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:42.748 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:42.778 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:43.198 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:43.228 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:43.648 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:43.678 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:44.048 -07 000000012 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.064349, 17-03-18 00:14:44.098 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:44.128 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:44.548 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:44.578 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:14:44.998 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:45.028 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:45.028 -07 000000610 INF: LanePump: Waited 19149 ms for pump motion to complete 17-03-18 00:14:45.028 -07 000000610 INF: =================== Pumping Reagent RMF (11) =================== 17-03-18 00:14:45.158 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:45.188 -07 000000610 INF: LanePump: Recv /0`2250(0x03)\r\n 17-03-18 00:14:45.188 -07 000000610 INF: LanePump: Current Position of Pump is: 2250 17-03-18 00:14:45.318 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:14:45.348 -07 000000610 INF: LanePump: Recv /0`2250(0x03)\r\n 17-03-18 00:14:45.348 -07 000000610 INF: LanePump: Current Position of Pump is: 2250 17-03-18 00:14:45.348 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:14:45.478 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:14:45.508 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:45.938 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:14:45.968 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:14:45.968 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:14:45.968 -07 000000610 INF: =================== =================== 17-03-18 00:14:45.968 -07 000000610 INF: =================== Flow Cell Temp 38° =================== 17-03-18 00:14:45.968 -07 000000610 INF: =================== Flow Cell Temp 38°, FC temperature to 38° =================== 17-03-18 00:14:45.968 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 38000000\n [FlowCellTempControl] 17-03-18 00:14:45.968 -07 000000429 INF: FPGA: Recv TEC2TEMP 17-03-18 00:14:45.978 -07 000000610 INF: FPGA: 'TEC2TEMP 0 38000000' command took 1 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:14:45.978 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:14:45.978 -07 000000501 INF: FPGA: Recv TEC2EN 17-03-18 00:14:45.988 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 8 ms overall 17-03-18 00:14:45.988 -07 000000610 INF: =================== Flow Cell Temp 38° =================== 17-03-18 00:14:45.988 -07 000000610 INF: =================== =================== 17-03-18 00:14:45.988 -07 000000610 INF: WaitCommand(300000) start 17-03-18 00:14:47.148 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 0 17-03-18 00:14:47.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=1, Total=1. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:14:49.408 -07 000000438 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.031682, 17-03-18 00:14:52.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:52.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:14:54.768 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.031682, 17-03-18 00:14:57.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:14:57.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:00.128 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:15:02.148 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:02.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:05.488 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.963579, 17-03-18 00:15:07.148 -07 000000438 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:07.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:10.848 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:15:12.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:12.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:16.208 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.951279, 17-03-18 00:15:17.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:17.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:21.568 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.948561, 17-03-18 00:15:22.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:22.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:26.928 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:15:27.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:27.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:32.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:32.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:32.288 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:15:37.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:37.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:37.648 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:15:42.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:42.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:43.008 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:15:47.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:47.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:48.368 -07 000000382 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:15:52.148 -07 000000382 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:52.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:53.728 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.03576, 17-03-18 00:15:57.148 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:15:57.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:15:59.088 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.037119, 17-03-18 00:16:02.148 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:02.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:04.448 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:16:07.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:07.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:09.808 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:16:12.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:12.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:15.528 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:16:17.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:17.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:20.908 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:16:22.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:22.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:26.268 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:16:27.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:27.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:31.648 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:16:32.448 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:32.448 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:37.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:37.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:37.788 -07 000000415 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.019446, 17-03-18 00:16:42.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:42.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:43.148 -07 000000415 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:16:47.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:47.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:48.508 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:16:52.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:52.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:54.538 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:16:57.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:16:57.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:16:59.898 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.018051, 17-03-18 00:17:02.148 -07 000000501 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:02.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:05.258 -07 000000501 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:17:07.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:07.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:10.618 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:17:12.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:12.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:15.978 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:17:17.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:17.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:21.338 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:17:22.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:22.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:26.698 -07 000000415 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:17:27.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:27.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:32.058 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:17:32.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:32.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:37.148 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:37.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:37.418 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:17:42.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:42.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:42.778 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:17:47.148 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:47.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:48.138 -07 000000415 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:17:52.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:52.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:54.048 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:17:57.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:17:57.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:17:59.408 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:18:02.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:02.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:04.768 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.0344, 17-03-18 00:18:07.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:07.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:10.128 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.043918, 17-03-18 00:18:12.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:12.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:15.488 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.033041, 17-03-18 00:18:17.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:17.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:20.848 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:18:22.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:22.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:26.208 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:18:27.148 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:27.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:31.568 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:18:32.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:32.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:36.928 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:18:37.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:37.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:42.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:42.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:42.288 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:18:47.148 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:47.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:47.648 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:18:52.148 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:52.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:53.008 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:18:57.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:18:57.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:18:58.368 -07 000000415 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:19:02.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:02.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:03.728 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.071149, 17-03-18 00:19:07.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:07.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:09.088 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:19:12.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:12.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:14.448 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:19:17.148 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:17.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:19.808 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.052111, 17-03-18 00:19:22.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:22.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:25.168 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.050752, 17-03-18 00:19:27.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:27.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:30.528 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:19:32.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:32.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:35.888 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:19:37.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:37.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:41.248 -07 000000458 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.954032, 17-03-18 00:19:42.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:42.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:46.008 -07 000000610 INF: WaitCommand(300000) end 17-03-18 00:19:46.008 -07 000000610 INF: =================== Pumping Reagent RMF (11) =================== 17-03-18 00:19:46.008 -07 000000610 INF: LanePump: Pumping from 11, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 25 µL, calling command Pumping to Flow Cell #1 17-03-18 00:19:46.098 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:19:46.128 -07 000000610 INF: LanePump: Recv /0`2250(0x03)\r\n 17-03-18 00:19:46.128 -07 000000610 INF: LanePump: Current Position of Pump is: 2250 17-03-18 00:19:46.128 -07 000000610 INF: =================== Pumping Reagent RMF (11), Aspirating port InputPort =================== 17-03-18 00:19:46.258 -07 000000610 INF: LanePump: Xmit /1V50IP150R\r 17-03-18 00:19:46.298 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:19:46.608 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:19:47.148 -07 000000458 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:47.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:51.218 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:19:51.248 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:19:51.668 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:19:51.698 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:19:51.968 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:19:52.118 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:19:52.148 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:19:52.148 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:52.148 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:52.568 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:19:52.598 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:19:53.018 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:19:53.048 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:19:53.468 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:19:53.498 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:19:53.498 -07 000000610 INF: LanePump: Waited 7199 ms for pump motion to complete 17-03-18 00:19:53.498 -07 000000610 INF: =================== Pumping Reagent RMF (11) =================== 17-03-18 00:19:53.628 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:19:53.658 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:19:53.658 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:19:53.788 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:19:53.818 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:19:53.818 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:19:53.818 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:19:53.948 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:19:53.978 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:19:54.408 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:19:54.438 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:19:54.438 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:19:54.438 -07 000000610 INF: =================== =================== 17-03-18 00:19:54.438 -07 000000610 INF: WaitCommand(300000) start 17-03-18 00:19:57.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:19:57.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:19:57.328 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:20:02.148 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:02.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:02.688 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:20:07.148 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:07.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:08.048 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:20:12.158 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:12.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:13.408 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:20:17.158 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:17.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:18.768 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:20:22.158 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:22.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:24.128 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:20:27.158 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:27.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:29.488 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:20:32.158 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:32.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:34.848 -07 000000551 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:20:37.158 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:37.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:40.208 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:20:42.158 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:42.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:45.568 -07 000000436 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:20:47.158 -07 000000436 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:47.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:50.928 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:20:52.158 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:52.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:20:56.958 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:20:57.158 -07 000000429 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:20:57.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:02.158 -07 000000436 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:02.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:02.318 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:21:07.158 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:07.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:07.678 -07 000000436 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:21:12.158 -07 000000551 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:12.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:13.038 -07 000000429 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:21:17.158 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:17.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:18.398 -07 000000415 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:21:22.158 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:22.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:23.758 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:21:27.158 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:27.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:29.118 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:21:32.218 -07 000000433 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:32.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:34.478 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:21:37.158 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:37.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:39.838 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:21:42.158 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:42.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:45.198 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:21:47.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:47.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:50.558 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:21:52.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:52.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:21:55.918 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:21:57.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:21:57.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:01.278 -07 000000433 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:22:02.158 -07 000000436 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:02.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:07.218 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:07.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:07.218 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:22:12.158 -07 000000512 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:12.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:12.578 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:22:17.158 -07 000000436 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:17.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:17.938 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:22:22.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:22.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:23.298 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:22:27.158 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:27.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:28.658 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:22:32.158 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:32.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:34.018 -07 000000512 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.037119, 17-03-18 00:22:37.158 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:37.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:39.378 -07 000000436 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:22:42.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:42.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:45.568 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:22:47.158 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:47.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:50.928 -07 000000415 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:22:52.158 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:52.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:22:56.288 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:22:57.158 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:22:57.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:01.648 -07 000000442 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:23:02.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:02.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:07.008 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:23:07.158 -07 000000442 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:07.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:12.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:12.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:12.368 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.03576, 17-03-18 00:23:17.158 -07 000000436 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:17.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:17.728 -07 000000436 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.046637, 17-03-18 00:23:22.158 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:22.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:23.088 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:23:27.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:27.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:28.448 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:23:32.158 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:32.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:34.818 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.970374, 17-03-18 00:23:37.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:37.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:40.178 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:23:42.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:42.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:45.538 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:23:47.218 -07 000000415 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:47.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:50.898 -07 000000436 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:23:52.158 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:52.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:23:56.258 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:23:57.158 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:23:57.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:01.618 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:24:02.158 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:02.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:06.978 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:24:07.158 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:07.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:12.158 -07 000000436 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:12.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:12.338 -07 000000436 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:24:17.158 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:17.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:17.698 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:24:22.158 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:22.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:23.058 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:24:27.158 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:27.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:28.418 -07 000000436 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:24:32.158 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:32.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:33.778 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:24:37.158 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:37.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:39.138 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:24:42.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:42.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:45.368 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:24:47.158 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:47.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:50.728 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:24:52.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:52.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:54.448 -07 000000610 INF: WaitCommand(300000) end 17-03-18 00:24:54.448 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:24:54.448 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:24:54.448 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:24:54.468 -07 000000610 INF: ReagentValve: Recv 0B\r 17-03-18 00:24:54.468 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 11 to 3. Moving 8 positions. 17-03-18 00:24:54.468 -07 000000610 INF: ReagentValve: Xmit -03\r 17-03-18 00:24:55.158 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:24:55.178 -07 000000610 INF: ReagentValve: Recv 0675\r 17-03-18 00:24:55.178 -07 000000610 INF: ReagentValve: Move- from 11 to 3 took 1653 steps, expected 1633. Error = 20 steps (1.2%) 17-03-18 00:24:55.228 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:24:55.248 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:24:55.248 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:24:55.348 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:24:55.348 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:24:55.348 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:24:55.378 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:24:55.378 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:24:55.508 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:24:55.538 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:24:55.538 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:24:57.048 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:24:57.158 -07 000000436 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:24:57.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:24:59.708 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:24:59.738 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:24:59.738 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:24:59.868 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:24:59.898 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:00.328 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:00.358 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:00.358 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:25:00.488 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:00.518 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:25:00.518 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:25:00.648 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:25:00.688 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:02.158 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:25:02.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:25:02.408 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:25:07.158 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:25:07.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:25:07.768 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:25:08.818 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:08.848 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:09.268 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:09.298 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:09.718 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:09.748 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:10.168 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:10.198 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:10.618 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:10.648 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:10.648 -07 000000610 INF: LanePump: Waited 9959 ms for pump motion to complete 17-03-18 00:25:10.648 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:25:10.778 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:25:10.818 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:12.158 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:25:12.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1338, R2=1390, Total=2728. Warn if over 250 bubbles per interval. 17-03-18 00:25:13.128 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:25:13.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:13.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:14.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:14.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:14.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:14.878 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:14.878 -07 000000610 INF: LanePump: Waited 4059 ms for pump motion to complete 17-03-18 00:25:14.878 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:25:15.008 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:15.038 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:25:15.038 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:25:15.168 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:15.198 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:25:15.198 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:25:15.198 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:25:15.328 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:25:15.358 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:15.788 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:15.818 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:15.818 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:25:15.818 -07 000000610 INF: =================== =================== 17-03-18 00:25:17.038 -07 000000610 INF: AsyncDelayTimer waited 1219ms 17-03-18 00:25:17.038 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:25:17.038 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:25:17.058 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:25:17.058 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 3 to 6. Moving 3 positions. 17-03-18 00:25:17.058 -07 000000610 INF: ReagentValve: Xmit +06\r 17-03-18 00:25:17.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 2 R1 1 17-03-18 00:25:17.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=1, R2=2, Total=3. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1339, R2=1392, Total=2731. Warn if over 250 bubbles per interval. 17-03-18 00:25:17.358 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:25:17.378 -07 000000610 INF: ReagentValve: Recv 0278\r 17-03-18 00:25:17.378 -07 000000610 INF: ReagentValve: Move+ from 3 to 6 took 632 steps, expected 612. Error = 20 steps (3.3%) 17-03-18 00:25:17.428 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:25:17.448 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:25:17.448 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:25:17.548 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:25:17.548 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 300 µL, calling command Pumping to Flow Cell #1 17-03-18 00:25:17.548 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:17.578 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:25:17.578 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:25:17.578 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:25:17.708 -07 000000610 INF: LanePump: Xmit /1V400IP1800R\r 17-03-18 00:25:17.748 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:18.488 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:25:22.158 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:25:22.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1339, R2=1392, Total=2731. Warn if over 250 bubbles per interval. 17-03-18 00:25:23.848 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:25:25.168 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:25.198 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:25.618 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:25.648 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:26.068 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:26.098 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:26.518 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:26.548 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:26.968 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:26.998 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:26.998 -07 000000610 INF: LanePump: Waited 9249 ms for pump motion to complete 17-03-18 00:25:26.998 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:25:27.128 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:27.158 -07 000000610 INF: LanePump: Recv /0`2520(0x03)\r\n 17-03-18 00:25:27.158 -07 000000610 INF: LanePump: Current Position of Pump is: 2520 17-03-18 00:25:27.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 3 R1 2 17-03-18 00:25:27.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=2, R2=3, Total=5. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1341, R2=1395, Total=2736. Warn if over 250 bubbles per interval. 17-03-18 00:25:27.288 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:27.318 -07 000000610 INF: LanePump: Recv /0`2520(0x03)\r\n 17-03-18 00:25:27.318 -07 000000610 INF: LanePump: Current Position of Pump is: 2520 17-03-18 00:25:27.318 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:25:27.448 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:25:27.478 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:27.908 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:27.938 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:27.938 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:25:27.938 -07 000000610 INF: =================== =================== 17-03-18 00:25:29.158 -07 000000610 INF: AsyncDelayTimer waited 1219ms 17-03-18 00:25:29.158 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:25:29.158 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:25:29.178 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:25:29.178 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 6 to 3. Moving 3 positions. 17-03-18 00:25:29.178 -07 000000610 INF: ReagentValve: Xmit -03\r 17-03-18 00:25:29.208 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:25:29.478 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:25:29.498 -07 000000610 INF: ReagentValve: Recv 0278\r 17-03-18 00:25:29.498 -07 000000610 INF: ReagentValve: Move- from 6 to 3 took 632 steps, expected 612. Error = 20 steps (3.3%) 17-03-18 00:25:29.548 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:25:29.568 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:25:29.568 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:25:29.668 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:25:29.668 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:25:29.668 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:29.698 -07 000000610 INF: LanePump: Recv /0`2520(0x03)\r\n 17-03-18 00:25:29.698 -07 000000610 INF: LanePump: Current Position of Pump is: 2520 17-03-18 00:25:29.828 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:29.858 -07 000000610 INF: LanePump: Recv /0`2520(0x03)\r\n 17-03-18 00:25:29.858 -07 000000610 INF: LanePump: Current Position of Pump is: 2520 17-03-18 00:25:32.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 2 17-03-18 00:25:32.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=2, R2=0, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1343, R2=1395, Total=2738. Warn if over 250 bubbles per interval. 17-03-18 00:25:34.028 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:34.058 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:34.058 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:25:34.188 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:25:34.218 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:34.568 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:25:34.648 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:34.678 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:34.678 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:25:34.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:34.838 -07 000000610 INF: LanePump: Recv /0`2520(0x03)\r\n 17-03-18 00:25:34.838 -07 000000610 INF: LanePump: Current Position of Pump is: 2520 17-03-18 00:25:34.968 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:25:34.998 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:37.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 2 R1 0 17-03-18 00:25:37.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=2, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1343, R2=1397, Total=2740. Warn if over 250 bubbles per interval. 17-03-18 00:25:39.928 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.028963, 17-03-18 00:25:42.158 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:25:42.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1343, R2=1397, Total=2740. Warn if over 250 bubbles per interval. 17-03-18 00:25:43.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:43.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:43.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:43.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:44.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:44.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:44.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:44.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:45.288 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:25:45.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:45.328 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:45.328 -07 000000610 INF: LanePump: Waited 10329 ms for pump motion to complete 17-03-18 00:25:45.328 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:25:45.458 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:25:45.498 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:47.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:25:47.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1343, R2=1397, Total=2740. Warn if over 250 bubbles per interval. 17-03-18 00:25:48.628 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:48.658 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:49.078 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:49.108 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:49.528 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:49.558 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:49.558 -07 000000610 INF: LanePump: Waited 4059 ms for pump motion to complete 17-03-18 00:25:49.558 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:25:49.688 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:49.718 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:25:49.718 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:25:49.848 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:49.878 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:25:49.878 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:25:49.878 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:25:50.008 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:25:50.038 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:50.468 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:50.498 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:25:50.498 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:25:50.498 -07 000000610 INF: =================== =================== 17-03-18 00:25:50.498 -07 000000610 INF: =================== Flow Cell Temp 50° =================== 17-03-18 00:25:50.498 -07 000000610 INF: =================== Flow Cell Temp 50°, FC temperature to 50° =================== 17-03-18 00:25:50.498 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 50000000\n [FlowCellTempControl] 17-03-18 00:25:50.498 -07 000000431 INF: FPGA: Recv TEC2TEMP 17-03-18 00:25:50.508 -07 000000610 INF: FPGA: 'TEC2TEMP 0 50000000' command took 1 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 00:25:50.508 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:25:50.508 -07 000000409 INF: FPGA: Recv TEC2EN 17-03-18 00:25:50.518 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:25:50.518 -07 000000610 INF: =================== Flow Cell Temp 50° =================== 17-03-18 00:25:50.518 -07 000000610 INF: =================== =================== 17-03-18 00:25:50.648 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.977204, 17-03-18 00:25:51.718 -07 000000610 INF: AsyncDelayTimer waited 1194ms 17-03-18 00:25:51.718 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:25:51.718 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:25:51.738 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:25:51.738 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 3 to 8. Moving 5 positions. 17-03-18 00:25:51.738 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:25:52.168 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 9 R1 10 17-03-18 00:25:52.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=10, R2=9, Total=19. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:25:52.198 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:25:52.218 -07 000000610 INF: ReagentValve: Recv 0413\r 17-03-18 00:25:52.218 -07 000000610 INF: ReagentValve: Move+ from 3 to 8 took 1043 steps, expected 1021. Error = 22 steps (2.2%) 17-03-18 00:25:52.268 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:25:52.288 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:25:52.288 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:25:52.388 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:25:52.388 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:25:52.388 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:25:52.418 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:25:52.418 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:25:52.418 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:25:52.548 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:25:52.588 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:56.008 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:25:57.168 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:25:57.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:25:58.048 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:58.078 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:58.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:58.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:58.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:58.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:59.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:59.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:25:59.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:25:59.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:00.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:00.328 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:00.328 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:26:00.328 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:26:00.458 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:00.488 -07 000000610 INF: LanePump: Recv /0`888(0x03)\r\n 17-03-18 00:26:00.488 -07 000000610 INF: LanePump: Current Position of Pump is: 888 17-03-18 00:26:00.618 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:00.648 -07 000000610 INF: LanePump: Recv /0`888(0x03)\r\n 17-03-18 00:26:00.648 -07 000000610 INF: LanePump: Current Position of Pump is: 888 17-03-18 00:26:00.648 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:26:00.778 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:26:00.808 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:01.238 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:01.268 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:01.268 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:26:01.268 -07 000000610 INF: =================== =================== 17-03-18 00:26:01.268 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:26:01.368 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:26:02.168 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:02.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:06.728 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:26:07.168 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:07.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:08.478 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:26:08.478 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:26:08.478 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:26:08.478 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:26:08.498 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:26:08.498 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:26:08.498 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:26:08.648 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:26:08.668 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:26:08.668 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:26:08.718 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:26:08.738 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:26:08.738 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:26:08.838 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:26:08.838 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:26:08.838 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:08.868 -07 000000610 INF: LanePump: Recv /0`888(0x03)\r\n 17-03-18 00:26:08.868 -07 000000610 INF: LanePump: Current Position of Pump is: 888 17-03-18 00:26:08.868 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:26:08.998 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:26:09.038 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:12.088 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:26:12.158 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:12.158 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:14.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:14.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:14.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:14.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:15.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:15.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:15.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:15.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:16.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:16.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:16.748 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:16.778 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:16.778 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:26:16.778 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:26:16.908 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:16.938 -07 000000610 INF: LanePump: Recv /0`1056(0x03)\r\n 17-03-18 00:26:16.938 -07 000000610 INF: LanePump: Current Position of Pump is: 1056 17-03-18 00:26:17.068 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:17.098 -07 000000610 INF: LanePump: Recv /0`1056(0x03)\r\n 17-03-18 00:26:17.098 -07 000000610 INF: LanePump: Current Position of Pump is: 1056 17-03-18 00:26:17.098 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:26:17.158 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:17.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:17.228 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:26:17.258 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:17.448 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:26:17.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:17.718 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:17.718 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:26:17.718 -07 000000610 INF: =================== =================== 17-03-18 00:26:17.718 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:26:22.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:22.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:22.808 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:26:24.928 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:26:24.928 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:26:24.928 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:26:24.928 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:26:24.948 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:26:24.948 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:26:24.948 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:26:25.078 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:26:25.098 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:26:25.098 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:26:25.148 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:26:25.168 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:26:25.168 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:26:25.268 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:26:25.268 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:26:25.268 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:25.298 -07 000000610 INF: LanePump: Recv /0`1056(0x03)\r\n 17-03-18 00:26:25.298 -07 000000610 INF: LanePump: Current Position of Pump is: 1056 17-03-18 00:26:25.298 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:26:25.428 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:26:25.468 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:27.158 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:27.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:28.168 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:26:32.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:32.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:33.528 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 00:26:37.168 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:37.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:38.888 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:26:41.168 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:41.198 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:41.618 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:41.648 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:42.068 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:42.098 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:42.168 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:42.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:42.518 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:42.548 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:42.968 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:42.998 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:43.418 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:43.448 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:26:43.868 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:43.898 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:43.898 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:26:43.898 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:26:44.028 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:44.058 -07 000000610 INF: LanePump: Recv /0`1488(0x03)\r\n 17-03-18 00:26:44.058 -07 000000610 INF: LanePump: Current Position of Pump is: 1488 17-03-18 00:26:44.188 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:26:44.218 -07 000000610 INF: LanePump: Recv /0`1488(0x03)\r\n 17-03-18 00:26:44.218 -07 000000610 INF: LanePump: Current Position of Pump is: 1488 17-03-18 00:26:44.218 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:26:44.248 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:26:44.348 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:26:44.378 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:44.808 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:26:44.838 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:26:44.838 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:26:44.838 -07 000000610 INF: =================== =================== 17-03-18 00:26:44.838 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:26:47.168 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:47.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:49.608 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:26:52.168 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:52.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:26:54.968 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.018051, 17-03-18 00:26:57.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:26:57.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:00.328 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.037119, 17-03-18 00:27:02.168 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:02.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:03.548 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:27:03.548 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:27:03.548 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:27:03.548 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:27:03.568 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:27:03.568 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:27:03.568 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:27:03.788 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:27:03.808 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:27:03.808 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:27:03.858 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:27:03.878 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:27:03.878 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:27:03.978 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:27:03.978 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:27:03.978 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:04.008 -07 000000610 INF: LanePump: Recv /0`1488(0x03)\r\n 17-03-18 00:27:04.008 -07 000000610 INF: LanePump: Current Position of Pump is: 1488 17-03-18 00:27:04.008 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:27:04.138 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:27:04.178 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:05.688 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:27:07.168 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:07.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:09.638 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:09.668 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:10.088 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:10.118 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:10.538 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:10.568 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:10.988 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:11.018 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:11.048 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:27:11.438 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:11.468 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:11.888 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:11.918 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:11.918 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:27:11.918 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:27:12.048 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:12.078 -07 000000610 INF: LanePump: Recv /0`1656(0x03)\r\n 17-03-18 00:27:12.078 -07 000000610 INF: LanePump: Current Position of Pump is: 1656 17-03-18 00:27:12.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:12.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:12.208 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:12.238 -07 000000610 INF: LanePump: Recv /0`1656(0x03)\r\n 17-03-18 00:27:12.238 -07 000000610 INF: LanePump: Current Position of Pump is: 1656 17-03-18 00:27:12.238 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:27:12.368 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:27:12.398 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:12.828 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:12.858 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:12.858 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:27:12.858 -07 000000610 INF: =================== =================== 17-03-18 00:27:12.858 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:27:16.408 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:27:17.168 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:17.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:20.068 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:27:20.068 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:27:20.068 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:27:20.068 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:27:20.088 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:27:20.088 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:27:20.088 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:27:20.238 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:27:20.258 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:27:20.258 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:27:20.308 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:27:20.328 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:27:20.328 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:27:20.428 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:27:20.428 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:27:20.428 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:20.458 -07 000000610 INF: LanePump: Recv /0`1656(0x03)\r\n 17-03-18 00:27:20.458 -07 000000610 INF: LanePump: Current Position of Pump is: 1656 17-03-18 00:27:20.458 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:27:20.588 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:27:20.628 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:21.768 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:27:22.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:22.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:26.088 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:26.118 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:26.538 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:26.568 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:26.988 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:27.018 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:27.128 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:27:27.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:27.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:27.438 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:27.468 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:27.888 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:27.918 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:28.338 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:28.368 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:28.368 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:27:28.368 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:27:28.498 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:28.528 -07 000000610 INF: LanePump: Recv /0`1824(0x03)\r\n 17-03-18 00:27:28.528 -07 000000610 INF: LanePump: Current Position of Pump is: 1824 17-03-18 00:27:28.658 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:28.688 -07 000000610 INF: LanePump: Recv /0`1824(0x03)\r\n 17-03-18 00:27:28.688 -07 000000610 INF: LanePump: Current Position of Pump is: 1824 17-03-18 00:27:28.688 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:27:28.818 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:27:28.848 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:29.278 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:29.308 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:29.308 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:27:29.308 -07 000000610 INF: =================== =================== 17-03-18 00:27:29.308 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:27:32.168 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:32.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:32.488 -07 000000431 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:27:36.518 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:27:36.518 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:27:36.518 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:27:36.518 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:27:36.538 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:27:36.538 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:27:36.538 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:27:36.678 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:27:36.698 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:27:36.698 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:27:36.748 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:27:36.768 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:27:36.768 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:27:36.868 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:27:36.868 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:27:36.868 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:36.898 -07 000000610 INF: LanePump: Recv /0`1824(0x03)\r\n 17-03-18 00:27:36.898 -07 000000610 INF: LanePump: Current Position of Pump is: 1824 17-03-18 00:27:36.898 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:27:37.028 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:27:37.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:37.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:37.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:37.848 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:27:42.168 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:42.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:43.208 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:27:47.168 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:47.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:48.568 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:27:52.168 -07 000000431 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:52.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:52.768 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:52.798 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:53.218 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:53.248 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:53.668 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:53.698 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:53.928 -07 000000459 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.018051, 17-03-18 00:27:54.118 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:54.148 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:54.568 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:54.598 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:55.018 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:55.048 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:27:55.468 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:55.498 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:55.498 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:27:55.498 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:27:55.628 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:55.658 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:27:55.658 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:27:55.788 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:27:55.818 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:27:55.818 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:27:55.818 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:27:55.948 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:27:55.978 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:56.408 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:27:56.438 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:27:56.438 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:27:56.438 -07 000000610 INF: =================== =================== 17-03-18 00:27:56.438 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:27:57.168 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:27:57.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:27:59.288 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:28:02.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:02.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:04.648 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:28:07.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:07.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:10.008 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.053471, 17-03-18 00:28:12.168 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:12.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:15.148 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:28:15.148 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:28:15.148 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:28:15.148 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:28:15.168 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:28:15.168 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:28:15.168 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:28:15.368 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:28:15.388 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:28:15.408 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:28:15.408 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:28:15.458 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:28:15.478 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:28:15.478 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:28:15.578 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:28:15.578 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:28:15.578 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:15.608 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:28:15.608 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:28:15.608 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:28:15.738 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:28:15.778 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:17.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:17.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:20.728 -07 000000475 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:28:21.238 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:21.268 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:21.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:21.718 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:22.138 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:22.168 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:22.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:22.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:22.588 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:22.618 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:23.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:23.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:23.488 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:23.518 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:23.518 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:28:23.518 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:28:23.648 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:23.678 -07 000000610 INF: LanePump: Recv /0`2424(0x03)\r\n 17-03-18 00:28:23.678 -07 000000610 INF: LanePump: Current Position of Pump is: 2424 17-03-18 00:28:23.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:23.838 -07 000000610 INF: LanePump: Recv /0`2424(0x03)\r\n 17-03-18 00:28:23.838 -07 000000610 INF: LanePump: Current Position of Pump is: 2424 17-03-18 00:28:23.838 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:28:23.968 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:28:23.998 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:24.428 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:24.458 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:24.458 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:28:24.458 -07 000000610 INF: =================== =================== 17-03-18 00:28:24.458 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:28:26.088 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:28:27.168 -07 000000475 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:27.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:31.448 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:28:31.668 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:28:31.668 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:28:31.668 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:28:31.668 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:28:31.688 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:28:31.688 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:28:31.688 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:28:31.838 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:28:31.858 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:28:31.858 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:28:31.908 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:28:31.928 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:28:31.928 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:28:32.028 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:28:32.028 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:28:32.028 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:32.058 -07 000000610 INF: LanePump: Recv /0`2424(0x03)\r\n 17-03-18 00:28:32.058 -07 000000610 INF: LanePump: Current Position of Pump is: 2424 17-03-18 00:28:32.058 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:28:32.168 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:32.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:32.188 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:28:32.228 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:36.808 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:28:37.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:37.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:37.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:37.718 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:38.138 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:38.168 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:38.588 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:38.618 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:39.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:39.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:39.488 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:39.518 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:39.938 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:39.968 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:39.968 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:28:39.968 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:28:40.098 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:40.128 -07 000000610 INF: LanePump: Recv /0`2592(0x03)\r\n 17-03-18 00:28:40.128 -07 000000610 INF: LanePump: Current Position of Pump is: 2592 17-03-18 00:28:40.258 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:40.288 -07 000000610 INF: LanePump: Recv /0`2592(0x03)\r\n 17-03-18 00:28:40.288 -07 000000610 INF: LanePump: Current Position of Pump is: 2592 17-03-18 00:28:40.288 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:28:40.418 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:28:40.448 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:40.878 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:40.908 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:40.908 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:28:40.908 -07 000000610 INF: =================== =================== 17-03-18 00:28:40.908 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:28:42.168 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:28:42.168 -07 000000459 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:42.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:47.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:47.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:47.528 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:28:48.118 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:28:48.118 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:28:48.118 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:28:48.118 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:28:48.138 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:28:48.138 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:28:48.138 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:28:48.268 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:28:48.288 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:28:48.288 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:28:48.338 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:28:48.358 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:28:48.358 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:28:48.458 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:28:48.458 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:28:48.458 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:48.488 -07 000000610 INF: LanePump: Recv /0`2592(0x03)\r\n 17-03-18 00:28:48.488 -07 000000610 INF: LanePump: Current Position of Pump is: 2592 17-03-18 00:28:48.618 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:48.648 -07 000000610 INF: LanePump: Recv /0`2592(0x03)\r\n 17-03-18 00:28:48.648 -07 000000610 INF: LanePump: Current Position of Pump is: 2592 17-03-18 00:28:52.168 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:52.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:52.818 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:52.848 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:52.848 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:28:52.888 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:28:52.978 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:28:53.008 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:53.438 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:28:53.468 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:28:53.468 -07 000000610 INF: LanePump: Waited 461 ms for pump motion to complete 17-03-18 00:28:53.598 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:28:53.628 -07 000000610 INF: LanePump: Recv /0`2592(0x03)\r\n 17-03-18 00:28:53.628 -07 000000610 INF: LanePump: Current Position of Pump is: 2592 17-03-18 00:28:53.758 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:28:53.798 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:28:57.168 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:28:57.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:28:58.248 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:29:02.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:02.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:02.588 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:02.618 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:03.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:03.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:03.488 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:03.518 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:03.608 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:29:03.938 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:03.968 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:04.388 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:04.418 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:29:04.418 -07 000000610 INF: LanePump: Waited 10619 ms for pump motion to complete 17-03-18 00:29:04.418 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:29:04.548 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:29:04.588 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:07.168 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:07.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:08.968 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:29:12.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:12.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:14.328 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:29:17.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:17.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:19.688 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.031682, 17-03-18 00:29:20.288 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:20.318 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:20.738 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:20.768 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:21.188 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:21.218 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:21.638 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:21.668 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:22.088 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:22.118 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:22.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:22.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:22.538 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:22.568 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:22.988 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:23.018 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:23.438 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:24.228 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:29:24.228 -07 000000610 INF: LanePump: Waited 19639 ms for pump motion to complete 17-03-18 00:29:24.228 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:29:24.358 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:29:24.388 -07 000000610 INF: LanePump: Recv /0`432(0x03)\r\n 17-03-18 00:29:24.388 -07 000000610 INF: LanePump: Current Position of Pump is: 432 17-03-18 00:29:24.518 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:29:24.548 -07 000000610 INF: LanePump: Recv /0`432(0x03)\r\n 17-03-18 00:29:24.548 -07 000000610 INF: LanePump: Current Position of Pump is: 432 17-03-18 00:29:24.548 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:29:24.678 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:29:24.708 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:29:25.048 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.028963, 17-03-18 00:29:25.138 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:25.168 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:29:25.168 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:29:25.168 -07 000000610 INF: =================== =================== 17-03-18 00:29:25.168 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:29:27.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:27.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:30.408 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:29:32.168 -07 000000562 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:32.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:35.768 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:29:37.168 -07 000000562 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:37.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:41.128 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:29:42.168 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:42.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:43.878 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:29:43.878 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:29:43.878 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:29:43.878 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:29:43.898 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:29:43.898 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:29:43.898 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:29:44.118 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:29:44.138 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:29:44.138 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:29:44.188 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:29:44.208 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:29:44.208 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:29:44.308 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:29:44.308 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:29:44.308 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:29:44.338 -07 000000610 INF: LanePump: Recv /0`432(0x03)\r\n 17-03-18 00:29:44.338 -07 000000610 INF: LanePump: Current Position of Pump is: 432 17-03-18 00:29:44.338 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:29:44.468 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:29:44.508 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:46.488 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:29:47.168 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:47.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:49.968 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:49.998 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:50.418 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:50.448 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:50.868 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:50.898 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:51.318 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:51.348 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:51.768 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:52.198 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:52.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:52.208 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:29:52.508 -07 000000562 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:29:52.628 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:52.658 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:29:52.658 -07 000000610 INF: LanePump: Waited 8149 ms for pump motion to complete 17-03-18 00:29:52.658 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:29:52.788 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:29:52.818 -07 000000610 INF: LanePump: Recv /0`600(0x03)\r\n 17-03-18 00:29:52.818 -07 000000610 INF: LanePump: Current Position of Pump is: 600 17-03-18 00:29:52.948 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:29:52.978 -07 000000610 INF: LanePump: Recv /0`600(0x03)\r\n 17-03-18 00:29:52.978 -07 000000610 INF: LanePump: Current Position of Pump is: 600 17-03-18 00:29:52.978 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:29:53.108 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:29:53.138 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:29:53.568 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:29:53.598 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:29:53.598 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:29:53.598 -07 000000610 INF: =================== =================== 17-03-18 00:29:53.598 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:29:57.168 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:29:57.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:29:57.868 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:30:00.808 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:30:00.808 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:30:00.808 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:30:00.808 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:30:00.828 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:30:00.828 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:30:00.828 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:30:00.978 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:30:00.998 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:30:00.998 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:30:01.048 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:30:01.068 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:30:01.068 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:30:01.168 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:30:01.168 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:30:01.168 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:30:01.198 -07 000000610 INF: LanePump: Recv /0`600(0x03)\r\n 17-03-18 00:30:01.198 -07 000000610 INF: LanePump: Current Position of Pump is: 600 17-03-18 00:30:01.198 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:30:01.328 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:30:01.368 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:02.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:02.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:03.228 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:30:06.828 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:06.858 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:07.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:07.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:07.278 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:07.308 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:07.728 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:07.758 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:08.178 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:08.208 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:08.628 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:08.658 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:09.078 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:09.108 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:30:09.108 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:30:09.108 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:30:09.238 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:30:09.268 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:30:09.268 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:30:09.398 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:30:09.428 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:30:09.428 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:30:09.428 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:30:09.558 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:30:09.558 -07 000000562 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:30:09.588 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:30:10.018 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:10.048 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:30:10.048 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:30:10.048 -07 000000610 INF: =================== =================== 17-03-18 00:30:10.048 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:30:12.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:12.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:14.928 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.956714, 17-03-18 00:30:17.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:17.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:17.258 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:30:17.258 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:30:17.258 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:30:17.258 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:30:17.278 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:30:17.278 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:30:17.278 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:30:17.418 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:30:17.438 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:30:17.438 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:30:17.488 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:30:17.508 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:30:17.508 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:30:17.608 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:30:17.608 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:30:17.608 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:30:17.638 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:30:17.638 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:30:17.638 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:30:17.768 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:30:17.808 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:20.298 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:30:22.168 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:22.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:25.658 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:30:27.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:27.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:31.018 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:30:32.168 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:32.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:33.508 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:33.538 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:33.958 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:33.988 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:34.408 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:34.438 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:34.858 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:34.888 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:35.308 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:35.338 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:35.758 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:35.788 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:36.208 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:36.238 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:30:36.238 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:30:36.238 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:30:36.368 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:30:36.378 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:30:36.398 -07 000000610 INF: LanePump: Recv /0`1200(0x03)\r\n 17-03-18 00:30:36.398 -07 000000610 INF: LanePump: Current Position of Pump is: 1200 17-03-18 00:30:36.528 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:30:36.558 -07 000000610 INF: LanePump: Recv /0`1200(0x03)\r\n 17-03-18 00:30:36.558 -07 000000610 INF: LanePump: Current Position of Pump is: 1200 17-03-18 00:30:36.558 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:30:36.688 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:30:36.718 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:30:37.148 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:30:37.168 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:37.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:37.178 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:30:37.178 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:30:37.178 -07 000000610 INF: =================== =================== 17-03-18 00:30:37.178 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:30:41.738 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:30:42.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:42.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:47.098 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:30:47.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:47.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:52.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:52.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:52.458 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:30:55.888 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:30:55.888 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:30:55.888 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:30:55.888 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:30:55.908 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:30:55.908 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:30:55.908 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:30:56.128 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:30:56.148 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:30:56.148 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:30:56.198 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:30:56.218 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:30:56.218 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:30:56.318 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:30:56.318 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:30:56.318 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:30:56.348 -07 000000610 INF: LanePump: Recv /0`1200(0x03)\r\n 17-03-18 00:30:56.348 -07 000000610 INF: LanePump: Current Position of Pump is: 1200 17-03-18 00:30:56.348 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:30:56.478 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:30:56.518 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:30:57.168 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:30:57.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:30:57.818 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:31:01.978 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:02.008 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:02.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:02.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:02.428 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:02.458 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:02.878 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:02.908 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:03.178 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:31:03.328 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:03.358 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:03.778 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:03.808 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:04.228 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:04.258 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:04.258 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:31:04.258 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:31:04.388 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:04.418 -07 000000610 INF: LanePump: Recv /0`1368(0x03)\r\n 17-03-18 00:31:04.418 -07 000000610 INF: LanePump: Current Position of Pump is: 1368 17-03-18 00:31:04.548 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:04.578 -07 000000610 INF: LanePump: Recv /0`1368(0x03)\r\n 17-03-18 00:31:04.578 -07 000000610 INF: LanePump: Current Position of Pump is: 1368 17-03-18 00:31:04.578 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:31:04.708 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:31:04.738 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:05.168 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:05.198 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:05.198 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:31:05.198 -07 000000610 INF: =================== =================== 17-03-18 00:31:05.198 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:31:07.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:07.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:08.538 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:31:12.168 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:12.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:12.408 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:31:12.408 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:31:12.408 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:31:12.408 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:31:12.428 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:31:12.428 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:31:12.428 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:31:12.578 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:31:12.598 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:31:12.598 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:31:12.648 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:31:12.668 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:31:12.668 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:31:12.768 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:31:12.768 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:31:12.768 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:12.808 -07 000000610 INF: LanePump: Recv /0`1368(0x03)\r\n 17-03-18 00:31:12.808 -07 000000610 INF: LanePump: Current Position of Pump is: 1368 17-03-18 00:31:12.808 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:31:12.938 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:31:12.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:13.898 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:31:17.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:17.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:18.438 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:18.468 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:18.888 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:18.918 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:19.258 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:31:19.338 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:19.368 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:19.788 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:19.818 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:20.238 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:20.268 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:20.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:20.718 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:20.718 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:31:20.718 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:31:20.848 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:20.878 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:31:20.878 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:31:21.008 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:21.038 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:31:21.038 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:31:21.038 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:31:21.168 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:31:21.198 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:21.628 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:21.658 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:21.658 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:31:21.658 -07 000000610 INF: =================== =================== 17-03-18 00:31:21.658 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:31:22.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:22.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:24.618 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.023489, 17-03-18 00:31:27.168 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:27.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:28.868 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:31:28.868 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:31:28.868 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:31:28.868 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:31:28.888 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:31:28.888 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:31:28.888 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:31:29.028 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:31:29.048 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:31:29.048 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:31:29.098 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:31:29.118 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:31:29.118 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:31:29.218 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:31:29.218 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:31:29.218 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:29.248 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:31:29.248 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:31:29.248 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:31:29.378 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:31:29.418 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:29.978 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:31:32.178 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:32.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:35.338 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:31:37.178 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:40.698 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:31:42.178 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:42.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:45.118 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:45.148 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:45.568 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:45.598 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:46.018 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:46.048 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:46.058 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:31:46.468 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:46.498 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:46.918 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:46.948 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:47.168 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:47.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:47.368 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:47.398 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:31:47.818 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:47.848 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:47.848 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:31:47.848 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:31:47.978 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:48.008 -07 000000610 INF: LanePump: Recv /0`1968(0x03)\r\n 17-03-18 00:31:48.008 -07 000000610 INF: LanePump: Current Position of Pump is: 1968 17-03-18 00:31:48.138 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:31:48.168 -07 000000610 INF: LanePump: Recv /0`1968(0x03)\r\n 17-03-18 00:31:48.168 -07 000000610 INF: LanePump: Current Position of Pump is: 1968 17-03-18 00:31:48.168 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:31:48.298 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:31:48.328 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:48.758 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:31:48.788 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:31:48.788 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:31:48.788 -07 000000610 INF: =================== =================== 17-03-18 00:31:48.788 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:31:51.418 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:31:52.168 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:52.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:31:56.778 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:31:57.168 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:31:57.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:02.138 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:32:02.178 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:02.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:07.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:07.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:07.498 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:32:07.498 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:32:07.498 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:32:07.498 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:32:07.498 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:32:07.518 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:32:07.518 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:32:07.518 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:32:07.738 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:32:07.758 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:32:07.758 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:32:07.808 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:32:07.828 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:32:07.828 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:32:07.928 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:32:07.928 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:32:07.928 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:07.958 -07 000000610 INF: LanePump: Recv /0`1968(0x03)\r\n 17-03-18 00:32:07.958 -07 000000610 INF: LanePump: Current Position of Pump is: 1968 17-03-18 00:32:07.958 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:32:08.088 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:32:08.128 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:12.178 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:12.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:12.858 -07 000000493 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:32:13.588 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:13.618 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:14.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:14.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:14.488 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:14.518 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:14.938 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:14.968 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:15.388 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:15.418 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:15.838 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:15.868 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:32:15.868 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:32:15.868 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:32:15.998 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:16.028 -07 000000610 INF: LanePump: Recv /0`2136(0x03)\r\n 17-03-18 00:32:16.028 -07 000000610 INF: LanePump: Current Position of Pump is: 2136 17-03-18 00:32:16.158 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:16.188 -07 000000610 INF: LanePump: Recv /0`2136(0x03)\r\n 17-03-18 00:32:16.188 -07 000000610 INF: LanePump: Current Position of Pump is: 2136 17-03-18 00:32:16.188 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:32:16.318 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:32:16.348 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:32:16.778 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:16.808 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:32:16.808 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:32:16.808 -07 000000610 INF: =================== =================== 17-03-18 00:32:16.808 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:32:17.168 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:17.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:18.218 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:32:22.168 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:22.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:23.578 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:32:24.018 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:32:24.018 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:32:24.018 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:32:24.018 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:32:24.038 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:32:24.038 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:32:24.038 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:32:24.188 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:32:24.208 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:32:24.208 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:32:24.258 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:32:24.278 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:32:24.278 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:32:24.378 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:32:24.378 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:32:24.378 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:24.408 -07 000000610 INF: LanePump: Recv /0`2136(0x03)\r\n 17-03-18 00:32:24.408 -07 000000610 INF: LanePump: Current Position of Pump is: 2136 17-03-18 00:32:24.408 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:32:24.538 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:32:24.578 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:27.168 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:27.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:28.938 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:32:30.038 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:30.068 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:30.488 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:30.518 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:30.938 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:30.968 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:31.388 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:31.418 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:31.838 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:31.868 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:32.168 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:32.168 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:32.288 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:32.318 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:32:32.318 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:32:32.318 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:32:32.448 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:32.478 -07 000000610 INF: LanePump: Recv /0`2304(0x03)\r\n 17-03-18 00:32:32.478 -07 000000610 INF: LanePump: Current Position of Pump is: 2304 17-03-18 00:32:32.608 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:32.638 -07 000000610 INF: LanePump: Recv /0`2304(0x03)\r\n 17-03-18 00:32:32.638 -07 000000610 INF: LanePump: Current Position of Pump is: 2304 17-03-18 00:32:32.638 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:32:32.768 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:32:32.798 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:32:33.228 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:33.258 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:32:33.258 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:32:33.258 -07 000000610 INF: =================== =================== 17-03-18 00:32:33.258 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:32:34.298 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:32:37.168 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:39.658 -07 000000558 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:32:40.468 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:32:40.468 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:32:40.468 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:32:40.468 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:32:40.488 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:32:40.488 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:32:40.488 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:32:40.618 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:32:40.638 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:32:40.638 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:32:40.688 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:32:40.708 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:32:40.708 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:32:40.808 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:32:40.808 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:32:40.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:40.838 -07 000000610 INF: LanePump: Recv /0`2304(0x03)\r\n 17-03-18 00:32:40.838 -07 000000610 INF: LanePump: Current Position of Pump is: 2304 17-03-18 00:32:40.838 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:32:40.968 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:32:41.008 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:42.168 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:42.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:45.018 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:32:47.178 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:47.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:50.378 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.05755, 17-03-18 00:32:52.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:52.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:55.738 -07 000000557 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.06163, 17-03-18 00:32:56.708 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:56.738 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:57.158 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:57.178 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:32:57.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:32:57.188 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:57.608 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:57.638 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:58.058 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:58.088 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:58.508 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:58.538 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:58.958 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:58.988 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:32:59.408 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:32:59.438 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:32:59.438 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:32:59.438 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:32:59.568 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:59.598 -07 000000610 INF: LanePump: Recv /0`2736(0x03)\r\n 17-03-18 00:32:59.598 -07 000000610 INF: LanePump: Current Position of Pump is: 2736 17-03-18 00:32:59.728 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:32:59.758 -07 000000610 INF: LanePump: Recv /0`2736(0x03)\r\n 17-03-18 00:32:59.758 -07 000000610 INF: LanePump: Current Position of Pump is: 2736 17-03-18 00:32:59.758 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:32:59.888 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:32:59.918 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:00.348 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:00.378 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:00.378 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:33:00.378 -07 000000610 INF: =================== =================== 17-03-18 00:33:00.378 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:33:01.098 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:33:02.178 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:02.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:06.458 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:33:07.178 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:07.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:11.818 -07 000000481 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:33:12.178 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:12.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:17.178 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:17.178 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.964938, 17-03-18 00:33:17.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:19.088 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:33:19.088 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:33:19.088 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:33:19.088 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:33:19.108 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:33:19.108 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:33:19.108 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:33:19.328 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:33:19.348 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:33:19.348 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:33:19.398 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:33:19.418 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:33:19.418 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:33:19.518 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:33:19.518 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:33:19.518 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:33:19.558 -07 000000610 INF: LanePump: Recv /0`2736(0x03)\r\n 17-03-18 00:33:19.558 -07 000000610 INF: LanePump: Current Position of Pump is: 2736 17-03-18 00:33:19.558 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:33:19.688 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:33:19.728 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:22.178 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:22.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:22.538 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:33:25.188 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:25.218 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:25.638 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:25.668 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:26.088 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:26.118 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:26.538 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:26.568 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:26.988 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:27.018 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:27.178 -07 000000556 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:27.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:27.438 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:27.468 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:27.468 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:33:27.468 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:33:27.598 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:33:27.628 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:33:27.628 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:33:27.758 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:33:27.788 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:33:27.788 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:33:27.788 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:33:27.898 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:33:27.918 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:33:27.948 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:28.378 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:28.408 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:28.408 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:33:28.408 -07 000000610 INF: =================== =================== 17-03-18 00:33:28.408 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:33:32.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:32.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:33.258 -07 000000493 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:33:35.618 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:33:35.618 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:33:35.618 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:33:35.618 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:33:35.638 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:33:35.638 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:33:35.638 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:33:35.788 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:33:35.808 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:33:35.808 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:33:35.858 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:33:35.878 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:33:35.878 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:33:35.978 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:33:35.978 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:33:35.978 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:33:36.008 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:33:36.008 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:33:36.138 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:33:36.168 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:33:36.168 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:33:37.178 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:38.618 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:33:40.338 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:40.368 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:40.368 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:33:40.498 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:33:40.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:40.958 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:40.988 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:40.988 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:33:41.118 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:33:41.148 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:33:41.148 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:33:41.278 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:33:41.318 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:42.178 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:42.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:43.978 -07 000000481 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:33:47.178 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:47.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:49.338 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.043918, 17-03-18 00:33:51.448 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:51.478 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:51.898 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:51.928 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:52.178 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:52.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:52.348 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:52.378 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:52.798 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:52.828 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:53.248 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:53.278 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:33:53.278 -07 000000610 INF: LanePump: Waited 11959 ms for pump motion to complete 17-03-18 00:33:53.278 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:33:53.408 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:33:53.448 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:54.698 -07 000000557 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:33:57.178 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:33:57.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:33:58.908 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:58.938 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:59.358 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:59.388 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:33:59.808 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:33:59.838 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:00.058 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:34:00.258 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:00.288 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:00.708 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:00.738 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:01.158 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:01.188 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:01.608 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:01.638 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:01.638 -07 000000610 INF: LanePump: Waited 8189 ms for pump motion to complete 17-03-18 00:34:01.638 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:34:01.768 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:01.798 -07 000000610 INF: LanePump: Recv /0`168(0x03)\r\n 17-03-18 00:34:01.798 -07 000000610 INF: LanePump: Current Position of Pump is: 168 17-03-18 00:34:01.928 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:01.958 -07 000000610 INF: LanePump: Recv /0`168(0x03)\r\n 17-03-18 00:34:01.958 -07 000000610 INF: LanePump: Current Position of Pump is: 168 17-03-18 00:34:01.958 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:34:02.088 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:34:02.118 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:02.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:02.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:02.548 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:02.578 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:02.578 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:34:02.578 -07 000000610 INF: =================== =================== 17-03-18 00:34:02.578 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:34:05.418 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.955391, 17-03-18 00:34:07.178 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:07.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:09.788 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:34:09.788 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:34:09.788 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:34:09.788 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:34:09.808 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:34:09.808 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:34:09.808 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:34:09.938 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:34:09.958 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:34:09.958 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:34:10.008 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:34:10.028 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:34:10.028 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:34:10.128 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:34:10.128 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:34:10.128 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:10.158 -07 000000610 INF: LanePump: Recv /0`168(0x03)\r\n 17-03-18 00:34:10.158 -07 000000610 INF: LanePump: Current Position of Pump is: 168 17-03-18 00:34:10.158 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:34:10.288 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:34:10.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:10.778 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.970374, 17-03-18 00:34:12.178 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:12.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:16.138 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:34:17.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:17.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:21.498 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.052111, 17-03-18 00:34:22.178 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:22.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:26.028 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:26.058 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:26.478 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:26.508 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:26.858 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.045313, 17-03-18 00:34:26.928 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:26.958 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:27.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:27.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:27.378 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:27.408 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:27.828 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:27.858 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:28.278 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:28.308 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:28.728 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:28.758 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:28.758 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:34:28.758 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:34:28.888 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:28.918 -07 000000610 INF: LanePump: Recv /0`600(0x03)\r\n 17-03-18 00:34:28.918 -07 000000610 INF: LanePump: Current Position of Pump is: 600 17-03-18 00:34:29.048 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:29.078 -07 000000610 INF: LanePump: Recv /0`600(0x03)\r\n 17-03-18 00:34:29.078 -07 000000610 INF: LanePump: Current Position of Pump is: 600 17-03-18 00:34:29.078 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:34:29.208 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:34:29.238 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:29.668 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:29.698 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:29.698 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:34:29.698 -07 000000610 INF: =================== =================== 17-03-18 00:34:29.698 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:34:32.178 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:32.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:32.218 -07 000000493 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.046637, 17-03-18 00:34:37.178 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:37.578 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.019446, 17-03-18 00:34:42.178 -07 000000554 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:42.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:42.938 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:34:47.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:47.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:48.298 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:34:48.408 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:34:48.408 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:34:48.408 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:34:48.408 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:34:48.428 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:34:48.428 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:34:48.428 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:34:48.648 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:34:48.668 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:34:48.668 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:34:48.718 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:34:48.738 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:34:48.738 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:34:48.838 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:34:48.838 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:34:48.838 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:48.868 -07 000000610 INF: LanePump: Recv /0`600(0x03)\r\n 17-03-18 00:34:48.868 -07 000000610 INF: LanePump: Current Position of Pump is: 600 17-03-18 00:34:48.868 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:34:48.998 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:34:49.038 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:52.178 -07 000000539 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:52.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:53.658 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:34:54.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:54.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:54.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:54.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:55.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:55.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:55.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:55.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:56.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:56.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:34:56.748 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:56.778 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:56.778 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:34:56.778 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:34:56.908 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:56.938 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:34:56.938 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:34:57.068 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:34:57.098 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:34:57.098 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:34:57.098 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:34:57.178 -07 000000493 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:34:57.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:34:57.228 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:34:57.258 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:57.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:34:57.718 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:34:57.718 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:34:57.718 -07 000000610 INF: =================== =================== 17-03-18 00:34:57.718 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:34:59.018 -07 000000556 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:35:02.178 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:02.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:04.378 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:35:04.928 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:35:04.928 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:35:04.928 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:35:04.928 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:35:04.948 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:35:04.948 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:35:04.948 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:35:05.098 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:35:05.118 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:35:05.118 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:35:05.168 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:35:05.188 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:35:05.188 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:35:05.288 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:35:05.288 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:35:05.288 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:35:05.318 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:35:05.318 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:35:05.318 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:35:05.448 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:35:05.488 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:07.178 -07 000000493 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:07.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:09.738 -07 000000558 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:35:10.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:10.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:11.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:11.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:11.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:11.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:12.178 -07 000000493 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:12.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:12.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:12.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:12.748 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:12.778 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:13.198 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:13.228 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:35:13.228 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:35:13.228 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:35:13.358 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:35:13.388 -07 000000610 INF: LanePump: Recv /0`936(0x03)\r\n 17-03-18 00:35:13.388 -07 000000610 INF: LanePump: Current Position of Pump is: 936 17-03-18 00:35:13.518 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:35:13.548 -07 000000610 INF: LanePump: Recv /0`936(0x03)\r\n 17-03-18 00:35:13.548 -07 000000610 INF: LanePump: Current Position of Pump is: 936 17-03-18 00:35:13.548 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:35:13.678 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:35:13.708 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:35:14.138 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:14.168 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:35:14.168 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:35:14.168 -07 000000610 INF: =================== =================== 17-03-18 00:35:14.168 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:35:15.098 -07 000000539 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:35:17.178 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:17.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:20.458 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:35:21.378 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:35:21.378 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:35:21.378 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:35:21.378 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:35:21.398 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:35:21.398 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:35:21.398 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:35:21.538 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:35:21.558 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:35:21.558 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:35:21.608 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:35:21.628 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:35:21.628 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:35:21.728 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:35:21.728 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:35:21.728 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:35:21.758 -07 000000610 INF: LanePump: Recv /0`936(0x03)\r\n 17-03-18 00:35:21.758 -07 000000610 INF: LanePump: Current Position of Pump is: 936 17-03-18 00:35:21.758 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:35:21.888 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:35:21.928 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:22.178 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:22.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:25.818 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:35:27.178 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:27.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:31.178 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:35:32.178 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:32.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:36.538 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:35:37.178 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:37.628 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:37.658 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:38.078 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:38.108 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:38.528 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:38.558 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:38.978 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:39.008 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:39.428 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:39.458 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:39.878 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:39.908 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:35:40.328 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:40.358 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:35:40.358 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:35:40.358 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:35:40.488 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:35:40.518 -07 000000610 INF: LanePump: Recv /0`1368(0x03)\r\n 17-03-18 00:35:40.518 -07 000000610 INF: LanePump: Current Position of Pump is: 1368 17-03-18 00:35:40.648 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:35:40.678 -07 000000610 INF: LanePump: Recv /0`1368(0x03)\r\n 17-03-18 00:35:40.678 -07 000000610 INF: LanePump: Current Position of Pump is: 1368 17-03-18 00:35:40.678 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:35:40.808 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:35:40.838 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:35:41.268 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:35:41.298 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:35:41.298 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:35:41.298 -07 000000610 INF: =================== =================== 17-03-18 00:35:41.298 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:35:41.898 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:35:42.178 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:42.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:47.178 -07 000000493 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:47.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:47.258 -07 000000493 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:35:52.178 -07 000000409 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:52.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:52.618 -07 000000554 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:35:57.178 -07 000000493 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:35:57.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:35:57.978 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.018051, 17-03-18 00:36:00.008 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:36:00.008 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:36:00.008 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:36:00.008 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:36:00.028 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:36:00.028 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:36:00.028 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:36:00.248 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:36:00.268 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:36:00.268 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:36:00.318 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:36:00.338 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:36:00.338 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:36:00.438 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:36:00.438 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:36:00.438 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:00.468 -07 000000610 INF: LanePump: Recv /0`1368(0x03)\r\n 17-03-18 00:36:00.468 -07 000000610 INF: LanePump: Current Position of Pump is: 1368 17-03-18 00:36:00.468 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:36:00.598 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:36:00.638 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:02.178 -07 000000493 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:02.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:03.338 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.028963, 17-03-18 00:36:06.098 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:06.128 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:06.548 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:06.578 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:06.998 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:07.028 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:07.178 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:07.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:07.448 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:07.478 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:07.898 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:07.928 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:08.348 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:08.378 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:08.378 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:36:08.378 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:36:08.508 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:08.538 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:36:08.538 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:36:08.668 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:08.698 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:36:08.698 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:36:08.698 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:36:08.698 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:36:08.828 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:36:08.858 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:09.288 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:09.318 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:09.318 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:36:09.318 -07 000000610 INF: =================== =================== 17-03-18 00:36:09.318 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:36:12.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:12.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:14.058 -07 000000409 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:36:16.528 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:36:16.528 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:36:16.528 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:36:16.528 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:36:16.548 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:36:16.548 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:36:16.548 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:36:16.698 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:36:16.718 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:36:16.718 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:36:16.768 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:36:16.788 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:36:16.788 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:36:16.888 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:36:16.888 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:36:16.888 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:16.918 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:36:16.918 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:36:16.918 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:36:17.048 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:36:17.088 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:17.178 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:17.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:19.418 -07 000000481 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:36:22.178 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:22.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:22.548 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:22.578 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:22.998 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:23.028 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:23.448 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:23.478 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:23.898 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:23.928 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:24.348 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:24.378 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:24.778 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:36:24.798 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:24.828 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:24.828 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:36:24.828 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:36:24.958 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:24.988 -07 000000610 INF: LanePump: Recv /0`1704(0x03)\r\n 17-03-18 00:36:24.988 -07 000000610 INF: LanePump: Current Position of Pump is: 1704 17-03-18 00:36:25.118 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:25.148 -07 000000610 INF: LanePump: Recv /0`1704(0x03)\r\n 17-03-18 00:36:25.148 -07 000000610 INF: LanePump: Current Position of Pump is: 1704 17-03-18 00:36:25.148 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:36:25.278 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:36:25.308 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:25.738 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:25.768 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:25.768 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:36:25.768 -07 000000610 INF: =================== =================== 17-03-18 00:36:25.768 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:36:27.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:27.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:30.138 -07 000000481 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:36:32.178 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:32.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:32.978 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:36:32.978 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:36:32.978 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:36:32.978 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:36:32.998 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:36:32.998 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:36:32.998 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:36:33.138 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:36:33.158 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:36:33.158 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:36:33.208 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:36:33.228 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:36:33.228 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:36:33.328 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:36:33.328 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:36:33.328 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:33.358 -07 000000610 INF: LanePump: Recv /0`1704(0x03)\r\n 17-03-18 00:36:33.358 -07 000000610 INF: LanePump: Current Position of Pump is: 1704 17-03-18 00:36:33.358 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:36:33.488 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:36:33.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:35.498 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:36:37.178 -07 000000493 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:40.858 -07 000000403 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:36:42.178 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:42.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:47.058 -07 000000493 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:36:47.178 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:47.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:49.228 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:49.258 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:49.678 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:49.708 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:50.128 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:50.158 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:50.578 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:50.608 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:51.028 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:51.058 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:51.478 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:51.508 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:36:51.928 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:51.958 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:51.958 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:36:51.958 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:36:52.088 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:52.118 -07 000000610 INF: LanePump: Recv /0`2136(0x03)\r\n 17-03-18 00:36:52.118 -07 000000610 INF: LanePump: Current Position of Pump is: 2136 17-03-18 00:36:52.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:52.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:52.248 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:36:52.278 -07 000000610 INF: LanePump: Recv /0`2136(0x03)\r\n 17-03-18 00:36:52.278 -07 000000610 INF: LanePump: Current Position of Pump is: 2136 17-03-18 00:36:52.278 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:36:52.408 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:36:52.418 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:36:52.438 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:52.868 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:36:52.898 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:36:52.898 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:36:52.898 -07 000000610 INF: =================== =================== 17-03-18 00:36:52.898 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:36:57.178 -07 000000403 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:36:57.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:36:58.808 -07 000000481 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:37:02.178 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:02.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:04.168 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:37:07.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:07.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:09.548 -07 000000428 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:37:11.608 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:37:11.608 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:37:11.608 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:37:11.608 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:37:11.628 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:37:11.628 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:37:11.628 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:37:11.848 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:37:11.868 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:37:11.868 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:37:11.918 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:37:11.938 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:37:11.938 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:37:12.038 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:37:12.038 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:37:12.038 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:37:12.068 -07 000000610 INF: LanePump: Recv /0`2136(0x03)\r\n 17-03-18 00:37:12.068 -07 000000610 INF: LanePump: Current Position of Pump is: 2136 17-03-18 00:37:12.068 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:37:12.178 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:12.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:12.198 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:37:12.238 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:14.908 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:37:17.188 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:17.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:17.698 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:17.728 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:18.148 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:18.178 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:18.598 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:18.628 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:19.048 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:19.078 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:19.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:19.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:19.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:19.978 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:37:19.978 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:37:19.978 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:37:20.108 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:37:20.138 -07 000000610 INF: LanePump: Recv /0`2304(0x03)\r\n 17-03-18 00:37:20.138 -07 000000610 INF: LanePump: Current Position of Pump is: 2304 17-03-18 00:37:20.268 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:37:20.278 -07 000000493 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.977204, 17-03-18 00:37:20.298 -07 000000610 INF: LanePump: Recv /0`2304(0x03)\r\n 17-03-18 00:37:20.298 -07 000000610 INF: LanePump: Current Position of Pump is: 2304 17-03-18 00:37:20.298 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:37:20.428 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:37:20.458 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:37:20.888 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:20.918 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:37:20.918 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:37:20.918 -07 000000610 INF: =================== =================== 17-03-18 00:37:20.918 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:37:22.178 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:22.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:25.648 -07 000000557 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 00:37:27.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:27.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:28.128 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:37:28.128 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:37:28.128 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:37:28.128 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:37:28.148 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:37:28.148 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:37:28.148 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:37:28.298 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:37:28.318 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:37:28.318 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:37:28.368 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:37:28.388 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:37:28.388 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:37:28.488 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:37:28.488 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:37:28.488 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:37:28.518 -07 000000610 INF: LanePump: Recv /0`2304(0x03)\r\n 17-03-18 00:37:28.518 -07 000000610 INF: LanePump: Current Position of Pump is: 2304 17-03-18 00:37:28.518 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:37:28.648 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:37:28.688 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:31.018 -07 000000595 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:37:32.178 -07 000000481 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:32.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:34.148 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:34.178 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:34.598 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:34.628 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:35.048 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:35.078 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:35.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:35.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:35.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:35.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:36.388 -07 000000499 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:37:36.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:36.428 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:37:36.428 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:37:36.428 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:37:36.558 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:37:36.588 -07 000000610 INF: LanePump: Recv /0`2472(0x03)\r\n 17-03-18 00:37:36.588 -07 000000610 INF: LanePump: Current Position of Pump is: 2472 17-03-18 00:37:36.718 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:37:36.748 -07 000000610 INF: LanePump: Recv /0`2472(0x03)\r\n 17-03-18 00:37:36.748 -07 000000610 INF: LanePump: Current Position of Pump is: 2472 17-03-18 00:37:36.748 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:37:36.878 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:37:36.908 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:37:37.178 -07 000000428 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:37.338 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:37:37.368 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:37:37.368 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:37:37.368 -07 000000610 INF: =================== =================== 17-03-18 00:37:37.368 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:37:41.758 -07 000000481 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:37:42.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:42.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:44.578 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:37:44.578 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:37:44.578 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:37:44.578 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:37:44.598 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:37:44.598 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:37:44.598 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:37:44.738 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:37:44.758 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:37:44.758 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:37:44.808 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:37:44.828 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:37:44.828 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:37:44.928 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:37:44.928 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:37:44.928 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:37:44.968 -07 000000610 INF: LanePump: Recv /0`2472(0x03)\r\n 17-03-18 00:37:44.968 -07 000000610 INF: LanePump: Current Position of Pump is: 2472 17-03-18 00:37:44.968 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:37:45.098 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:37:45.138 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:37:47.128 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:37:47.188 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:47.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:52.188 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:52.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:52.508 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:37:57.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:37:57.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:37:57.868 -07 000000481 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:38:00.838 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:00.868 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:01.288 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:01.318 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:01.738 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:01.768 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:02.178 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:02.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:02.188 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:02.218 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:02.638 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:02.668 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:03.088 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:03.118 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:03.238 -07 000000557 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:38:03.538 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:03.568 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:03.568 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:38:03.568 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:38:03.698 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:03.728 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:38:03.728 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:38:03.858 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:03.888 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:38:03.888 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:38:03.888 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:38:04.018 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:38:04.048 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:04.478 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:04.508 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:04.508 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:38:04.508 -07 000000610 INF: =================== =================== 17-03-18 00:38:04.508 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:38:07.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:07.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:08.608 -07 000000557 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:38:12.178 -07 000000557 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:12.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:13.978 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.966297, 17-03-18 00:38:17.178 -07 000000595 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:17.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:19.358 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:38:22.178 -07 000000595 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:22.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:23.218 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:38:23.218 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:38:23.218 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:38:23.218 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:38:23.238 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:38:23.238 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:38:23.238 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:38:23.458 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:38:23.478 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:38:23.478 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:38:23.528 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:38:23.548 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:38:23.548 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:38:23.648 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:38:23.648 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:38:23.648 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:23.678 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:38:23.678 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:38:23.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:23.838 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:38:23.838 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:38:24.718 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:38:27.178 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:27.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:28.008 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:28.038 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:28.038 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:38:28.168 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:38:28.198 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:28.628 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:28.658 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:28.658 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:38:28.788 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:28.818 -07 000000610 INF: LanePump: Recv /0`2904(0x03)\r\n 17-03-18 00:38:28.818 -07 000000610 INF: LanePump: Current Position of Pump is: 2904 17-03-18 00:38:28.948 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:38:28.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:30.088 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:38:32.178 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:32.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:35.458 -07 000000417 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.977204, 17-03-18 00:38:37.178 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:37.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:39.108 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:39.138 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:39.558 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:39.588 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:40.008 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:40.038 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:40.458 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:40.488 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:40.828 -07 000000417 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:38:40.908 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:40.938 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:40.938 -07 000000610 INF: LanePump: Waited 11959 ms for pump motion to complete 17-03-18 00:38:40.938 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:38:41.068 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:38:41.108 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:42.698 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:42.698 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:46.198 -07 000000557 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:38:46.568 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:46.598 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:47.018 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:47.048 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:47.178 -07 000000595 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:47.178 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:47.468 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:47.498 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:47.918 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:47.948 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:48.368 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:48.398 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:48.818 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:48.848 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:38:49.268 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:49.298 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:49.298 -07 000000610 INF: LanePump: Waited 8189 ms for pump motion to complete 17-03-18 00:38:49.298 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:38:49.428 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:49.458 -07 000000610 INF: LanePump: Recv /0`168(0x03)\r\n 17-03-18 00:38:49.458 -07 000000610 INF: LanePump: Current Position of Pump is: 168 17-03-18 00:38:49.588 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:49.618 -07 000000610 INF: LanePump: Recv /0`168(0x03)\r\n 17-03-18 00:38:49.618 -07 000000610 INF: LanePump: Current Position of Pump is: 168 17-03-18 00:38:49.618 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:38:49.748 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:38:49.778 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:50.208 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:38:50.238 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:38:50.238 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:38:50.238 -07 000000610 INF: =================== =================== 17-03-18 00:38:50.238 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:38:51.568 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.042558, 17-03-18 00:38:52.178 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:52.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:56.938 -07 000000557 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.027603, 17-03-18 00:38:57.178 -07 000000625 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:38:57.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:38:57.448 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:38:57.448 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:38:57.448 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:38:57.448 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:38:57.468 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:38:57.468 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:38:57.468 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:38:57.618 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:38:57.638 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:38:57.638 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:38:57.688 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:38:57.708 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:38:57.708 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:38:57.808 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:38:57.808 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:38:57.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:38:57.838 -07 000000610 INF: LanePump: Recv /0`168(0x03)\r\n 17-03-18 00:38:57.838 -07 000000610 INF: LanePump: Current Position of Pump is: 168 17-03-18 00:38:57.838 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:38:57.968 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:38:58.008 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:02.188 -07 000000595 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:02.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:02.308 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.037119, 17-03-18 00:39:03.468 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:03.498 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:03.918 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:03.948 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:04.368 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:04.398 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:04.818 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:04.848 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:05.268 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:05.298 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:05.718 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:05.748 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:39:05.748 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:39:05.748 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:39:05.878 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:39:05.908 -07 000000610 INF: LanePump: Recv /0`336(0x03)\r\n 17-03-18 00:39:05.908 -07 000000610 INF: LanePump: Current Position of Pump is: 336 17-03-18 00:39:06.038 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:39:06.068 -07 000000610 INF: LanePump: Recv /0`336(0x03)\r\n 17-03-18 00:39:06.068 -07 000000610 INF: LanePump: Current Position of Pump is: 336 17-03-18 00:39:06.068 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:39:06.198 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:39:06.228 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:39:06.658 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:06.688 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:39:06.688 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:39:06.688 -07 000000610 INF: =================== =================== 17-03-18 00:39:06.688 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:39:07.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:07.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:07.678 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:39:12.188 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:12.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:13.048 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:39:13.898 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:39:13.898 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:39:13.898 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:39:13.898 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:39:13.918 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:39:13.918 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:39:13.918 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:39:14.058 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:39:14.078 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:39:14.078 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:39:14.128 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:39:14.148 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:39:14.148 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:39:14.248 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:39:14.248 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:39:14.248 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:39:14.278 -07 000000610 INF: LanePump: Recv /0`336(0x03)\r\n 17-03-18 00:39:14.278 -07 000000610 INF: LanePump: Current Position of Pump is: 336 17-03-18 00:39:14.278 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:39:14.408 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:39:14.448 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:17.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:17.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:18.418 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:39:22.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:22.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:23.788 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:39:27.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:27.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:29.158 -07 000000444 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:39:30.148 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:30.178 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:30.598 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:30.628 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:31.048 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:31.078 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:31.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:31.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:31.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:31.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:32.188 -07 000000595 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:32.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:32.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:32.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:32.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:32.878 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:39:32.878 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:39:32.878 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:39:33.008 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:39:33.038 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:39:33.038 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:39:33.168 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:39:33.198 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:39:33.198 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:39:33.198 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:39:33.328 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:39:33.358 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:39:33.788 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:33.818 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:39:33.818 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:39:33.818 -07 000000610 INF: =================== =================== 17-03-18 00:39:33.818 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:39:34.538 -07 000000595 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 00:39:37.188 -07 000000595 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:37.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:39.898 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:39:42.188 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:42.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:45.268 -07 000000417 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:39:47.188 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:47.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:50.638 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:39:52.188 -07 000000595 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:52.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:52.528 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:39:52.528 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:39:52.528 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:39:52.528 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:39:52.548 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:39:52.548 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 6 to 8. Moving 2 positions. 17-03-18 00:39:52.548 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:39:52.768 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:39:52.788 -07 000000610 INF: ReagentValve: Recv 01AA\r 17-03-18 00:39:52.788 -07 000000610 INF: ReagentValve: Move+ from 6 to 8 took 426 steps, expected 408. Error = 18 steps (4.4%) 17-03-18 00:39:52.838 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:39:52.858 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:39:52.858 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:39:52.958 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:39:52.958 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:39:52.958 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:39:52.988 -07 000000610 INF: LanePump: Recv /0`768(0x03)\r\n 17-03-18 00:39:52.988 -07 000000610 INF: LanePump: Current Position of Pump is: 768 17-03-18 00:39:52.988 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:39:53.118 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:39:53.158 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:56.008 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:39:57.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:39:57.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:39:58.618 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:58.648 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:59.068 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:59.098 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:59.518 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:59.548 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:39:59.968 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:39:59.998 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:00.418 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:00.448 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:00.868 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:00.898 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:00.898 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:40:00.898 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:40:01.028 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:01.058 -07 000000610 INF: LanePump: Recv /0`936(0x03)\r\n 17-03-18 00:40:01.058 -07 000000610 INF: LanePump: Current Position of Pump is: 936 17-03-18 00:40:01.188 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:01.218 -07 000000610 INF: LanePump: Recv /0`936(0x03)\r\n 17-03-18 00:40:01.218 -07 000000610 INF: LanePump: Current Position of Pump is: 936 17-03-18 00:40:01.218 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:40:01.348 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:40:01.378 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:01.378 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.964938, 17-03-18 00:40:01.808 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:01.838 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:01.838 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:40:01.838 -07 000000610 INF: =================== =================== 17-03-18 00:40:01.838 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:40:02.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:02.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:06.738 -07 000000417 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 00:40:07.188 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:07.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:09.048 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:40:09.048 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:40:09.048 -07 000000610 INF: ReagentValve: Go to position 7 - PumpToFlowcell start 17-03-18 00:40:09.048 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:40:09.068 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:40:09.068 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 8 to 7. Moving 1 positions. 17-03-18 00:40:09.068 -07 000000610 INF: ReagentValve: Xmit -07\r 17-03-18 00:40:09.218 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:40:09.238 -07 000000610 INF: ReagentValve: Recv 00DD\r 17-03-18 00:40:09.238 -07 000000610 INF: ReagentValve: Move- from 8 to 7 took 221 steps, expected 204. Error = 17 steps (8.3%) 17-03-18 00:40:09.288 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:40:09.308 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:40:09.308 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:40:09.408 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:40:09.408 -07 000000610 INF: LanePump: Pumping from 7, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 28 µL, calling command Pumping to Flow Cell #1 17-03-18 00:40:09.408 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:09.438 -07 000000610 INF: LanePump: Recv /0`936(0x03)\r\n 17-03-18 00:40:09.438 -07 000000610 INF: LanePump: Current Position of Pump is: 936 17-03-18 00:40:09.438 -07 000000610 INF: =================== Pumping Reagent LPM (7), Aspirating port InputPort =================== 17-03-18 00:40:09.568 -07 000000610 INF: LanePump: Xmit /1V50IP168R\r 17-03-18 00:40:09.608 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:12.098 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:40:12.188 -07 000000463 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:12.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:15.068 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:15.098 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:15.518 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:15.548 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:15.968 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:15.998 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:16.418 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:16.448 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:16.868 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:16.898 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:17.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:17.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:17.318 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:17.348 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:17.348 -07 000000610 INF: LanePump: Waited 7739 ms for pump motion to complete 17-03-18 00:40:17.348 -07 000000610 INF: =================== Pumping Reagent LPM (7) =================== 17-03-18 00:40:17.458 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:40:17.478 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:17.508 -07 000000610 INF: LanePump: Recv /0`1104(0x03)\r\n 17-03-18 00:40:17.508 -07 000000610 INF: LanePump: Current Position of Pump is: 1104 17-03-18 00:40:17.638 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:17.668 -07 000000610 INF: LanePump: Recv /0`1104(0x03)\r\n 17-03-18 00:40:17.668 -07 000000610 INF: LanePump: Current Position of Pump is: 1104 17-03-18 00:40:17.668 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:40:17.798 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:40:17.828 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:18.258 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:18.288 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:18.288 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:40:18.288 -07 000000610 INF: =================== =================== 17-03-18 00:40:18.288 -07 000000610 INF: WaitCommand(7200) start 17-03-18 00:40:22.188 -07 000000625 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:22.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:22.818 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:40:25.498 -07 000000610 INF: WaitCommand(7200) end 17-03-18 00:40:25.498 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:40:25.498 -07 000000610 INF: ReagentValve: Go to position 6 - PumpToFlowcell start 17-03-18 00:40:25.498 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:40:25.518 -07 000000610 INF: ReagentValve: Recv 07\r 17-03-18 00:40:25.518 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 7 to 6. Moving 1 positions. 17-03-18 00:40:25.518 -07 000000610 INF: ReagentValve: Xmit -06\r 17-03-18 00:40:25.648 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:40:25.668 -07 000000610 INF: ReagentValve: Recv 00CD\r 17-03-18 00:40:25.668 -07 000000610 INF: ReagentValve: Move- from 7 to 6 took 205 steps, expected 204. Error = 1 steps (0.5%) 17-03-18 00:40:25.718 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:40:25.738 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:40:25.738 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:40:25.838 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:40:25.838 -07 000000610 INF: LanePump: Pumping from 6, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 72 µL, calling command Pumping to Flow Cell #1 17-03-18 00:40:25.838 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:25.868 -07 000000610 INF: LanePump: Recv /0`1104(0x03)\r\n 17-03-18 00:40:25.868 -07 000000610 INF: LanePump: Current Position of Pump is: 1104 17-03-18 00:40:25.868 -07 000000610 INF: =================== Pumping Reagent AMS2 (6), Aspirating port InputPort =================== 17-03-18 00:40:25.998 -07 000000610 INF: LanePump: Xmit /1V50IP432R\r 17-03-18 00:40:26.038 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:27.188 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:27.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:28.178 -07 000000417 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 00:40:32.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:32.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:33.538 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:40:37.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:37.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:38.898 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:40:41.738 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:41.768 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:42.188 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:42.188 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:42.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:42.218 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:42.638 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:42.668 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:43.088 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:43.118 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:43.538 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:43.568 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:43.988 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:44.018 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:40:44.258 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:40:44.438 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:44.468 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:44.468 -07 000000610 INF: LanePump: Waited 18429 ms for pump motion to complete 17-03-18 00:40:44.468 -07 000000610 INF: =================== Pumping Reagent AMS2 (6) =================== 17-03-18 00:40:44.598 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:44.628 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:40:44.628 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:40:44.758 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:40:44.788 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:40:44.788 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:40:44.788 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:40:44.918 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:40:44.948 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:45.378 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:40:45.408 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:40:45.408 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:40:45.408 -07 000000610 INF: =================== =================== 17-03-18 00:40:45.408 -07 000000610 INF: WaitCommand(18700) start 17-03-18 00:40:47.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:47.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:49.618 -07 000000451 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:40:52.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:52.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:40:54.978 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:40:57.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:40:57.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:41:00.338 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:41:02.718 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:02.718 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1353, R2=1406, Total=2759. Warn if over 250 bubbles per interval. 17-03-18 00:41:04.118 -07 000000610 INF: WaitCommand(18700) end 17-03-18 00:41:04.118 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:41:04.118 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:41:04.118 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:41:04.138 -07 000000610 INF: ReagentValve: Recv 06\r 17-03-18 00:41:04.138 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 6 to 3. Moving 3 positions. 17-03-18 00:41:04.138 -07 000000610 INF: ReagentValve: Xmit -03\r 17-03-18 00:41:04.428 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:41:04.448 -07 000000610 INF: ReagentValve: Recv 0269\r 17-03-18 00:41:04.448 -07 000000610 INF: ReagentValve: Move- from 6 to 3 took 617 steps, expected 612. Error = 5 steps (0.8%) 17-03-18 00:41:04.498 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:41:04.518 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:41:04.518 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:41:04.618 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:41:04.618 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:41:04.618 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:41:04.648 -07 000000610 INF: LanePump: Recv /0`1536(0x03)\r\n 17-03-18 00:41:04.648 -07 000000610 INF: LanePump: Current Position of Pump is: 1536 17-03-18 00:41:04.648 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:41:04.778 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:41:04.818 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:41:05.698 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:41:07.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 2 R1 4 17-03-18 00:41:07.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=4, R2=2, Total=6. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1357, R2=1408, Total=2765. Warn if over 250 bubbles per interval. 17-03-18 00:41:07.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:41:07.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:41:08.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:41:08.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:41:08.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:41:08.878 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:41:08.878 -07 000000610 INF: LanePump: Waited 4059 ms for pump motion to complete 17-03-18 00:41:08.878 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:41:09.008 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:41:09.038 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:41:09.038 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:41:09.168 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:41:09.198 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:41:09.198 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:41:09.198 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:41:09.328 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:41:09.358 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:41:09.788 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:41:09.818 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:41:09.818 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:41:09.818 -07 000000610 INF: =================== =================== 17-03-18 00:41:09.818 -07 000000610 INF: =================== Flow Cell Temp 20° =================== 17-03-18 00:41:09.818 -07 000000610 INF: =================== Flow Cell Temp 20°, FC temperature to 20° =================== 17-03-18 00:41:09.818 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 20000000\n [FlowCellTempControl] 17-03-18 00:41:09.818 -07 000000618 INF: FPGA: Recv TEC2TEMP 17-03-18 00:41:09.828 -07 000000610 INF: FPGA: 'TEC2TEMP 0 20000000' command took 1 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:41:09.828 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:41:09.828 -07 000000417 INF: FPGA: Recv TEC2EN 17-03-18 00:41:09.838 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:41:09.838 -07 000000610 INF: =================== Flow Cell Temp 20° =================== 17-03-18 00:41:09.838 -07 000000610 INF: WaitCommand(45000) start 17-03-18 00:41:11.058 -07 000000489 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:41:12.188 -07 000000489 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 4 R1 2 17-03-18 00:41:12.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=2, R2=4, Total=6. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:16.418 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:41:17.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:17.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:22.468 -07 000000489 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:22.468 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:22.468 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:41:27.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:27.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:27.828 -07 000000489 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:41:32.188 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:32.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:33.188 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:41:37.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:37.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:38.548 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:41:42.188 -07 000000489 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:42.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:44.618 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:41:47.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:47.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:49.988 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:41:52.188 -07 000000451 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:52.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:41:54.848 -07 000000610 INF: WaitCommand(45000) end 17-03-18 00:41:54.848 -07 000000610 INF: =================== =================== 17-03-18 00:41:54.848 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:41:54.848 -07 000000610 INF: ReagentValve: Go to position 10 - PumpToFlowcell start 17-03-18 00:41:54.848 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:41:54.868 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:41:54.868 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 3 to 10. Moving 7 positions. 17-03-18 00:41:54.868 -07 000000610 INF: ReagentValve: Xmit +0A\r 17-03-18 00:41:55.358 -07 000000417 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:41:55.478 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:41:56.108 -07 000000610 INF: ReagentValve: Recv 05A9\r 17-03-18 00:41:56.108 -07 000000610 INF: ReagentValve: Move+ from 3 to 10 took 1449 steps, expected 1429. Error = 20 steps (1.4%) 17-03-18 00:41:56.158 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:41:56.178 -07 000000610 INF: ReagentValve: Recv 0A\r 17-03-18 00:41:56.178 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:41:56.278 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:41:56.278 -07 000000610 INF: LanePump: Pumping from 10, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 300 µL, calling command Pumping to Flow Cell #1 17-03-18 00:41:56.278 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:41:56.318 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:41:56.318 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:41:56.448 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:41:56.478 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:41:56.478 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:41:57.188 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:41:57.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:42:00.648 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:00.678 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:00.678 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:42:00.728 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:42:00.808 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:42:00.838 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:01.268 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:01.298 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:01.298 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:42:01.428 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:42:01.458 -07 000000610 INF: LanePump: Recv /0`2256(0x03)\r\n 17-03-18 00:42:01.458 -07 000000610 INF: LanePump: Current Position of Pump is: 2256 17-03-18 00:42:01.588 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:42:01.628 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:02.188 -07 000000417 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:42:02.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:42:06.088 -07 000000444 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:42:07.188 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:42:07.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:42:09.078 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:09.108 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:09.528 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:09.558 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:09.978 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:10.008 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:10.428 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:10.458 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:10.878 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:10.908 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:10.908 -07 000000610 INF: LanePump: Waited 9279 ms for pump motion to complete 17-03-18 00:42:10.908 -07 000000610 INF: =================== Pumping Reagent LMX2 (10), Aspirating port InputPort =================== 17-03-18 00:42:11.038 -07 000000610 INF: LanePump: Xmit /1V400IP1800R\r 17-03-18 00:42:11.078 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:11.448 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:42:12.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:42:12.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1359, R2=1412, Total=2771. Warn if over 250 bubbles per interval. 17-03-18 00:42:16.808 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:42:17.188 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 4 R1 5 17-03-18 00:42:17.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=5, R2=4, Total=9. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1364, R2=1416, Total=2780. Warn if over 250 bubbles per interval. 17-03-18 00:42:18.498 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:18.528 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:18.948 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:18.978 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:19.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:19.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:19.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:19.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:20.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:20.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:20.748 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:20.778 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:20.778 -07 000000610 INF: LanePump: Waited 9699 ms for pump motion to complete 17-03-18 00:42:20.778 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:42:20.908 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:42:20.938 -07 000000610 INF: LanePump: Recv /0`1800(0x03)\r\n 17-03-18 00:42:20.938 -07 000000610 INF: LanePump: Current Position of Pump is: 1800 17-03-18 00:42:21.068 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:42:21.098 -07 000000610 INF: LanePump: Recv /0`1800(0x03)\r\n 17-03-18 00:42:21.098 -07 000000610 INF: LanePump: Current Position of Pump is: 1800 17-03-18 00:42:21.098 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:42:21.228 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:42:21.258 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:21.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:21.718 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:21.718 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 00:42:21.718 -07 000000610 INF: =================== =================== 17-03-18 00:42:21.718 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:42:21.718 -07 000000610 INF: LanePump: Pumping from 10, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 75 µL, calling command Pumping to Flow Cell #1 17-03-18 00:42:21.848 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:42:21.878 -07 000000610 INF: LanePump: Recv /0`1800(0x03)\r\n 17-03-18 00:42:21.878 -07 000000610 INF: LanePump: Current Position of Pump is: 1800 17-03-18 00:42:21.878 -07 000000610 INF: =================== Pumping Reagent LMX2 (10), Aspirating port InputPort =================== 17-03-18 00:42:22.008 -07 000000610 INF: LanePump: Xmit /1V50IP450R\r 17-03-18 00:42:22.048 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:22.168 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:42:22.188 -07 000000605 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 4 R1 6 17-03-18 00:42:22.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=6, R2=4, Total=10. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1370, R2=1420, Total=2790. Warn if over 250 bubbles per interval. 17-03-18 00:42:27.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 2 R1 0 17-03-18 00:42:27.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=2, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1370, R2=1422, Total=2792. Warn if over 250 bubbles per interval. 17-03-18 00:42:27.528 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:42:32.188 -07 000000444 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 2 17-03-18 00:42:32.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=2, R2=1, Total=3. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1372, R2=1423, Total=2795. Warn if over 250 bubbles per interval. 17-03-18 00:42:32.888 -07 000000605 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:42:37.188 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 0 17-03-18 00:42:37.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=1, Total=1. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1372, R2=1424, Total=2796. Warn if over 250 bubbles per interval. 17-03-18 00:42:38.248 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:42:38.468 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:38.498 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:38.918 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:38.948 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:39.368 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:39.398 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:39.818 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:39.848 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:40.268 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:40.298 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:40.718 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:40.748 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:42:41.168 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:41.198 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:41.198 -07 000000610 INF: LanePump: Waited 19149 ms for pump motion to complete 17-03-18 00:42:41.198 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:42:41.328 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:42:41.368 -07 000000610 INF: LanePump: Recv /0`2250(0x03)\r\n 17-03-18 00:42:41.368 -07 000000610 INF: LanePump: Current Position of Pump is: 2250 17-03-18 00:42:41.498 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:42:41.528 -07 000000610 INF: LanePump: Recv /0`2250(0x03)\r\n 17-03-18 00:42:41.528 -07 000000610 INF: LanePump: Current Position of Pump is: 2250 17-03-18 00:42:41.528 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:42:41.658 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:42:41.688 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:42.118 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:42:42.148 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:42:42.148 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:42:42.148 -07 000000610 INF: =================== =================== 17-03-18 00:42:42.148 -07 000000610 INF: =================== Flow Cell Temp 38° =================== 17-03-18 00:42:42.148 -07 000000610 INF: =================== Flow Cell Temp 38°, FC temperature to 38° =================== 17-03-18 00:42:42.148 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 38000000\n [FlowCellTempControl] 17-03-18 00:42:42.148 -07 000000618 INF: FPGA: Recv TEC2TEMP 17-03-18 00:42:42.158 -07 000000610 INF: FPGA: 'TEC2TEMP 0 38000000' command took 1 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:42:42.158 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:42:42.158 -07 000000447 INF: FPGA: Recv TEC2EN 17-03-18 00:42:42.168 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:42:42.168 -07 000000610 INF: =================== Flow Cell Temp 38° =================== 17-03-18 00:42:42.168 -07 000000610 INF: =================== =================== 17-03-18 00:42:42.168 -07 000000610 INF: WaitCommand(300000) start 17-03-18 00:42:42.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 1 17-03-18 00:42:42.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=1, R2=0, Total=1. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1373, R2=1424, Total=2797. Warn if over 250 bubbles per interval. 17-03-18 00:42:43.608 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:42:47.188 -07 000000625 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 1 17-03-18 00:42:47.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=1, R2=1, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:42:48.968 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:42:52.188 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:42:52.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:42:54.328 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:42:57.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:42:57.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:42:59.688 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:43:02.188 -07 000000444 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:02.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:05.048 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:43:07.188 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:07.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:10.408 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:43:12.188 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:12.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:15.768 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.975845, 17-03-18 00:43:17.188 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:17.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:21.128 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:43:22.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:22.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:27.148 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:43:27.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:27.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:32.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:32.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:32.508 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:43:37.188 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:37.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:37.868 -07 000000444 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:43:42.188 -07 000000444 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:42.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:43.228 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:43:47.188 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:47.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:48.588 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:43:52.198 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:52.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:53.948 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:43:57.198 -07 000000625 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:43:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:43:59.308 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:44:02.218 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:02.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:04.668 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:44:07.188 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:07.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:10.028 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.02213, 17-03-18 00:44:12.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:12.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:15.388 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:44:17.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:17.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:20.748 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:44:22.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:22.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:26.108 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:44:27.188 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:27.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:31.468 -07 000000625 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.027603, 17-03-18 00:44:32.188 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:32.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:36.828 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:44:37.638 -07 000000444 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:37.638 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:42.188 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:42.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:42.978 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.966297, 17-03-18 00:44:47.188 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:47.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:48.338 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:44:52.188 -07 000000444 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:52.188 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:53.698 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:44:57.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:44:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:44:59.888 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:45:02.188 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:02.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:05.248 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:45:07.188 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:07.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:11.338 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.970374, 17-03-18 00:45:12.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:12.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:16.698 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:45:17.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:17.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:22.068 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:45:22.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:22.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:27.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:27.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:28.108 -07 000000444 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.041198, 17-03-18 00:45:32.198 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:32.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:33.468 -07 000000444 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:45:37.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:37.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:38.838 -07 000000444 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 00:45:42.198 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:42.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:44.208 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:45:47.198 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:47.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:49.578 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:45:52.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:52.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:45:54.958 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:45:57.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:45:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:00.318 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.02213, 17-03-18 00:46:02.198 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:02.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:06.478 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:46:07.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:07.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:11.838 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:46:12.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:12.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:17.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:17.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:17.208 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:46:22.198 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:22.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:22.578 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 00:46:27.198 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:27.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:27.938 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:46:32.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:32.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:33.298 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:46:37.198 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:37.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:38.658 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:46:42.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:42.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:44.698 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:46:47.198 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:47.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:50.058 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:46:52.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:52.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:46:55.418 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:46:57.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:46:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:00.778 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:47:02.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:02.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:06.138 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.033041, 17-03-18 00:47:07.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:07.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:11.498 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:47:12.378 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:12.378 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:17.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:17.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:17.728 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.969015, 17-03-18 00:47:22.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:22.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:23.098 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:47:27.198 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:27.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:28.478 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.030322, 17-03-18 00:47:32.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:32.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:33.838 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:47:37.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:37.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:39.208 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:47:42.188 -07 000000610 INF: WaitCommand(300000) end 17-03-18 00:47:42.188 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:47:42.188 -07 000000610 INF: LanePump: Pumping from 10, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 25 µL, calling command Pumping to Flow Cell #1 17-03-18 00:47:42.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:42.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:42.278 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:47:42.308 -07 000000610 INF: LanePump: Recv /0`2250(0x03)\r\n 17-03-18 00:47:42.308 -07 000000610 INF: LanePump: Current Position of Pump is: 2250 17-03-18 00:47:42.308 -07 000000610 INF: =================== Pumping Reagent LMX2 (10), Aspirating port InputPort =================== 17-03-18 00:47:42.438 -07 000000610 INF: LanePump: Xmit /1V50IP150R\r 17-03-18 00:47:42.478 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:47:44.578 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:47:47.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:47.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:47.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:47:47.428 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:47:47.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:47:47.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:47:48.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:47:48.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:47:48.748 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:47:48.778 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:47:49.198 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:47:49.228 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:47:49.648 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:47:49.678 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:47:49.678 -07 000000610 INF: LanePump: Waited 7199 ms for pump motion to complete 17-03-18 00:47:49.678 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:47:49.808 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:47:49.838 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:47:49.838 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:47:49.958 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:47:49.968 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:47:49.998 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:47:49.998 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:47:49.998 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:47:50.128 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:47:50.158 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:47:50.588 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:47:50.618 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:47:50.618 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:47:50.618 -07 000000610 INF: =================== =================== 17-03-18 00:47:50.618 -07 000000610 INF: WaitCommand(300000) start 17-03-18 00:47:52.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:52.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:47:55.318 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:47:57.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:47:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:00.688 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:48:02.198 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:02.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:06.058 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:48:07.198 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:07.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:12.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:12.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:12.398 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:48:17.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:17.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:18.428 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:48:22.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:22.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:24.078 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.963579, 17-03-18 00:48:27.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:27.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:29.448 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:48:32.198 -07 000000480 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:32.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:34.818 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:48:37.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:37.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:40.718 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 00:48:42.198 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:42.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:46.368 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.030322, 17-03-18 00:48:47.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:47.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:52.438 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:52.438 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:52.438 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.027603, 17-03-18 00:48:57.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:48:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:48:57.798 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:49:02.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:02.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:03.158 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:49:07.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:07.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:08.518 -07 000000480 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.952638, 17-03-18 00:49:12.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:12.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:14.538 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:49:17.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:17.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:19.898 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:49:22.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:22.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:25.258 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.027603, 17-03-18 00:49:27.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:27.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:30.618 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.030322, 17-03-18 00:49:32.198 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:32.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:36.768 -07 000000558 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.047997, 17-03-18 00:49:37.198 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:37.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:42.128 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.047997, 17-03-18 00:49:42.198 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:42.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:47.198 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:47.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:47.488 -07 000000461 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.02213, 17-03-18 00:49:52.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:52.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:52.848 -07 000000461 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:49:57.198 -07 000000461 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:49:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:49:58.208 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.963579, 17-03-18 00:50:02.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:02.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:03.568 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:50:07.198 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:07.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:08.928 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:50:12.198 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:12.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:14.288 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:50:17.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:17.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:19.648 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:50:22.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:22.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:25.008 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:50:27.198 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:27.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:31.208 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:50:32.198 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:32.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:36.568 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:50:37.568 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:37.568 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:41.928 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:50:42.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:42.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:47.198 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:47.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:47.288 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:50:52.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:52.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:52.648 -07 000000487 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:50:57.198 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:50:57.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:50:58.008 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.969015, 17-03-18 00:51:02.198 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:02.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:03.368 -07 000000558 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.973092, 17-03-18 00:51:07.198 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:07.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:08.728 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:51:12.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:12.198 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:14.088 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.007176, 17-03-18 00:51:17.198 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:17.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:19.448 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.031682, 17-03-18 00:51:22.198 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:22.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:24.808 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:51:27.208 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:27.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:30.168 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:51:33.078 -07 000000447 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:33.078 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:35.528 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:51:37.208 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:37.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:40.888 -07 000000447 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.962185, 17-03-18 00:51:42.208 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:42.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:46.248 -07 000000590 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.962185, 17-03-18 00:51:47.208 -07 000000623 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:47.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:51.608 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:51:52.208 -07 000000463 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:52.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:51:56.968 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:51:57.208 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:51:57.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:02.208 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:02.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:02.328 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:52:07.208 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:07.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:07.688 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:52:12.208 -07 000000487 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:12.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:13.048 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:52:17.208 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:17.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:18.408 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.019446, 17-03-18 00:52:22.208 -07 000000590 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:22.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:23.768 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.027603, 17-03-18 00:52:27.208 -07 000000623 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:27.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:29.128 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:52:32.208 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:32.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:34.488 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:52:37.208 -07 000000623 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:37.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:39.848 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.977204, 17-03-18 00:52:42.208 -07 000000463 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:42.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:45.208 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:52:47.208 -07 000000623 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:47.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:50.568 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:52:50.628 -07 000000610 INF: WaitCommand(300000) end 17-03-18 00:52:50.628 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:52:50.628 -07 000000610 INF: LanePump: Pumping from 10, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 25 µL, calling command Pumping to Flow Cell #1 17-03-18 00:52:50.748 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:52:50.778 -07 000000610 INF: LanePump: Recv /0`2400(0x03)\r\n 17-03-18 00:52:50.778 -07 000000610 INF: LanePump: Current Position of Pump is: 2400 17-03-18 00:52:50.778 -07 000000610 INF: =================== Pumping Reagent LMX2 (10), Aspirating port InputPort =================== 17-03-18 00:52:50.908 -07 000000610 INF: LanePump: Xmit /1V50IP150R\r 17-03-18 00:52:50.948 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:52:52.208 -07 000000471 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:52.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:55.868 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:52:55.898 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:52:55.928 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:52:56.318 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:52:56.348 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:52:56.768 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:52:56.798 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:52:57.208 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:52:57.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:52:57.218 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:52:57.248 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:52:57.668 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:52:57.698 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:52:58.118 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:52:58.148 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:52:58.148 -07 000000610 INF: LanePump: Waited 7199 ms for pump motion to complete 17-03-18 00:52:58.148 -07 000000610 INF: =================== Pumping Reagent LMX2 (10) =================== 17-03-18 00:52:58.278 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:52:58.308 -07 000000610 INF: LanePump: Recv /0`2550(0x03)\r\n 17-03-18 00:52:58.308 -07 000000610 INF: LanePump: Current Position of Pump is: 2550 17-03-18 00:52:58.438 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:52:58.468 -07 000000610 INF: LanePump: Recv /0`2550(0x03)\r\n 17-03-18 00:52:58.468 -07 000000610 INF: LanePump: Current Position of Pump is: 2550 17-03-18 00:52:58.468 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:52:58.598 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:52:58.628 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:52:59.058 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:52:59.088 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:52:59.088 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:52:59.088 -07 000000610 INF: =================== =================== 17-03-18 00:52:59.088 -07 000000610 INF: WaitCommand(300000) start 17-03-18 00:53:01.288 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:53:02.208 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:02.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:06.648 -07 000000471 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:53:07.208 -07 000000593 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:07.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:12.008 -07 000000593 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.004458, 17-03-18 00:53:12.208 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:12.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:17.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:17.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:18.218 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.024884, 17-03-18 00:53:22.208 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:22.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:23.578 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.018051, 17-03-18 00:53:27.208 -07 000000463 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:27.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:28.938 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:53:32.208 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:32.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:34.298 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.023489, 17-03-18 00:53:37.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:37.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:39.658 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:53:42.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:42.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:45.018 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:53:47.208 -07 000000479 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:47.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:50.378 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.963579, 17-03-18 00:53:52.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:52.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:53:55.738 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.969015, 17-03-18 00:53:57.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:53:57.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:01.098 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.970374, 17-03-18 00:54:02.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:02.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:06.458 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:54:07.208 -07 000000623 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:07.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:11.818 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.030322, 17-03-18 00:54:12.208 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:12.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:17.178 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.03576, 17-03-18 00:54:17.208 -07 000000618 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:17.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:22.208 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:22.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:22.538 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:54:27.208 -07 000000579 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:27.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:27.898 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.001704, 17-03-18 00:54:32.208 -07 000000479 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:32.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:33.258 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.971733, 17-03-18 00:54:37.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:37.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:38.618 -07 000000463 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 00:54:42.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:42.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:43.978 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:54:47.218 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:47.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:49.338 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:54:52.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:52.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:54:54.698 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:54:57.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:54:57.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:00.058 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:55:02.208 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:02.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:05.418 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:55:07.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:07.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:10.778 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:55:12.208 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:12.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:16.138 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:55:17.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:17.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:21.498 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 00:55:22.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:22.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:26.928 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:55:27.208 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:27.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:32.208 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:32.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:32.288 -07 000000629 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.028963, 17-03-18 00:55:37.208 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:37.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:37.648 -07 000000623 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:55:42.208 -07 000000623 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:42.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:43.698 -07 000000618 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:55:47.208 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:47.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:49.078 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:55:52.208 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:52.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:55:54.438 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:55:57.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:55:57.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:00.158 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:56:02.208 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:02.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:05.788 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:56:07.208 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:07.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:11.168 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.019446, 17-03-18 00:56:12.208 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:12.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:16.528 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.020806, 17-03-18 00:56:17.208 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:17.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:22.298 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:22.298 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:22.298 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.979922, 17-03-18 00:56:27.208 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:27.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:27.968 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.011254, 17-03-18 00:56:32.208 -07 000000579 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:32.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:33.998 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 00:56:37.208 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:37.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:39.358 -07 000000629 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.994908, 17-03-18 00:56:42.208 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:42.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:44.738 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.003063, 17-03-18 00:56:47.208 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:47.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:50.098 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.009895, 17-03-18 00:56:52.208 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:52.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:56:55.478 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:56:57.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:56:57.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:00.838 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 00:57:02.208 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:02.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:06.478 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:57:07.208 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:07.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:12.148 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:57:12.208 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:12.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:17.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:17.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:17.528 -07 000000629 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.988112, 17-03-18 00:57:22.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:22.208 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:23.388 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.99219, 17-03-18 00:57:27.208 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:27.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:28.748 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.997626, 17-03-18 00:57:32.208 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:32.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:34.108 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.98264, 17-03-18 00:57:37.208 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:37.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:39.468 -07 000000629 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:57:42.208 -07 000000579 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:42.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:45.368 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:57:47.218 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:47.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:50.728 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.028963, 17-03-18 00:57:52.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:52.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:56.088 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.983999, 17-03-18 00:57:57.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:57:57.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:57:59.098 -07 000000610 INF: WaitCommand(300000) end 17-03-18 00:57:59.098 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:57:59.098 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 00:57:59.098 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:57:59.118 -07 000000610 INF: ReagentValve: Recv 0A\r 17-03-18 00:57:59.118 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 10 to 3. Moving 7 positions. 17-03-18 00:57:59.118 -07 000000610 INF: ReagentValve: Xmit -03\r 17-03-18 00:57:59.728 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:57:59.748 -07 000000610 INF: ReagentValve: Recv 05A9\r 17-03-18 00:57:59.748 -07 000000610 INF: ReagentValve: Move- from 10 to 3 took 1449 steps, expected 1429. Error = 20 steps (1.4%) 17-03-18 00:57:59.798 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:57:59.818 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:57:59.818 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:57:59.918 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:57:59.918 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 00:57:59.918 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:57:59.948 -07 000000610 INF: LanePump: Recv /0`2550(0x03)\r\n 17-03-18 00:57:59.948 -07 000000610 INF: LanePump: Current Position of Pump is: 2550 17-03-18 00:58:00.078 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:58:00.108 -07 000000610 INF: LanePump: Recv /0`2550(0x03)\r\n 17-03-18 00:58:00.108 -07 000000610 INF: LanePump: Current Position of Pump is: 2550 17-03-18 00:58:02.218 -07 000000579 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:02.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:58:02.228 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.005817, 17-03-18 00:58:04.278 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:04.308 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:04.308 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 00:58:04.438 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 00:58:04.468 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:04.898 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:04.928 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:04.928 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:58:05.058 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:58:05.088 -07 000000610 INF: LanePump: Recv /0`2550(0x03)\r\n 17-03-18 00:58:05.088 -07 000000610 INF: LanePump: Current Position of Pump is: 2550 17-03-18 00:58:05.218 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 00:58:05.258 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:07.218 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:07.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:58:07.588 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.031682, 17-03-18 00:58:12.218 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:12.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1425, Total=2799. Warn if over 250 bubbles per interval. 17-03-18 00:58:13.448 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.000345, 17-03-18 00:58:13.878 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:13.908 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:14.328 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:14.358 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:14.778 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:14.808 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:15.228 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:15.258 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:15.678 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:15.708 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:15.708 -07 000000610 INF: LanePump: Waited 10449 ms for pump motion to complete 17-03-18 00:58:15.708 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 00:58:15.838 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 00:58:15.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:17.218 -07 000000437 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 0 17-03-18 00:58:17.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=1, Total=1. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1374, R2=1426, Total=2800. Warn if over 250 bubbles per interval. 17-03-18 00:58:18.808 -07 000000437 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:58:19.008 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:19.038 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:19.458 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:19.488 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:19.908 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:19.938 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:19.938 -07 000000610 INF: LanePump: Waited 4059 ms for pump motion to complete 17-03-18 00:58:19.938 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 00:58:20.068 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:58:20.098 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:58:20.098 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:58:20.228 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:58:20.258 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:58:20.258 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:58:20.258 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:58:20.388 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:58:20.418 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:20.848 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:20.878 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:20.878 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:58:20.878 -07 000000610 INF: =================== =================== 17-03-18 00:58:20.948 -07 000000610 INF: Y Motor: Entering MoveAbsStepped (target = 120609 steps, sync = True, waitForSettling = True) 17-03-18 00:58:20.948 -07 000000610 INF: Y Motor: Moving to 75.3806 mm, started at 75.3833 mm, delta = -0.0027 mm 17-03-18 00:58:20.948 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:58:20.948 -07 000000579 INF: FPGA: Recv YRDPOS 154385 17-03-18 00:58:20.958 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 5 ms overall 17-03-18 00:58:20.958 -07 000000610 INF: Y Motor: Encoder = 154385 = motor step 120613.3 = 75.3833 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:58:20.958 -07 000000610 INF: Y Motor: Last commanded position = 75.3806 mm (120608.9 steps), delta from last commanded position = 0.0000 mm (0.0 steps). UsingTableMove = False 17-03-18 00:58:20.958 -07 000000610 INF: Y Motor: Moving to 154379 encoder counts 17-03-18 00:58:20.958 -07 000000610 INF: FPGA: Xmit YMVTO 154379\n [Y Motor] 17-03-18 00:58:20.988 -07 000000579 INF: FPGA: Recv YMVTO 154380 11 17-03-18 00:58:20.988 -07 000000610 INF: FPGA: 'YMVTO 154379' command took 0 ms to send, 28 ms to get response, and 37 ms overall 17-03-18 00:58:20.988 -07 000000610 INF: Y Motor: Position error before settling = 1 encoder counts (0.000 mm) 17-03-18 00:58:20.988 -07 000000610 INF: Y Motor: To move from 75.383 to 75.381 (-0.003 mm) should take 5 steps, but required 11 steps (0.007 mm). Slippage = 0.004 mm (-1.38%). 17-03-18 00:58:20.988 -07 000000610 INF: Y Motor: FPGA move command 'YMVTO 154379' took 38 ms, distance = 0.003 mm, average velocity = 0.072 mm/sec 17-03-18 00:58:22.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 1 R1 1 17-03-18 00:58:22.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=1, R2=1, Total=2. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:58:23.988 -07 000000610 INF: FPGA: Xmit YRDPOS\n [Y Motor] 17-03-18 00:58:23.988 -07 000000437 INF: FPGA: Recv YRDPOS 154372 17-03-18 00:58:23.998 -07 000000610 INF: FPGA: 'YRDPOS' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:58:23.998 -07 000000610 INF: Y Motor: Encoder = 154372 = motor step 120603.1 = 75.3770 mm (step is ~0.63 um, encoder count is ~0.49 um) 17-03-18 00:58:23.998 -07 000000610 INF: Y Motor: After move to 75.380580, error = -0.003627, min/max error = -0.022606/0.004186, tolerance = 0.500000 mm 17-03-18 00:58:23.998 -07 000000610 INF: Y Motor: Position error after settling = -7 encoder counts (-0.003 mm), Delta during settling = -8 counts (-0.004 mm) 17-03-18 00:58:23.998 -07 000000610 INF: Y Motor: Moved to 75.3770 after 3049 ms 17-03-18 00:58:24.008 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:58:24.008 -07 000000610 INF: ReagentValve: Go to position 8 - PumpToFlowcell start 17-03-18 00:58:24.008 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:58:24.028 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 00:58:24.028 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 3 to 8. Moving 5 positions. 17-03-18 00:58:24.028 -07 000000610 INF: ReagentValve: Xmit +08\r 17-03-18 00:58:24.168 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:58:24.488 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:58:24.508 -07 000000610 INF: ReagentValve: Recv 0413\r 17-03-18 00:58:24.508 -07 000000610 INF: ReagentValve: Move+ from 3 to 8 took 1043 steps, expected 1021. Error = 22 steps (2.2%) 17-03-18 00:58:24.558 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:58:24.578 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:58:24.578 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:58:24.678 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:58:24.678 -07 000000610 INF: LanePump: Pumping from 8, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 75 µL, calling command Pumping to Flow Cell #1 17-03-18 00:58:24.678 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:58:24.708 -07 000000610 INF: LanePump: Recv /0`720(0x03)\r\n 17-03-18 00:58:24.708 -07 000000610 INF: LanePump: Current Position of Pump is: 720 17-03-18 00:58:24.708 -07 000000610 INF: =================== Pumping Reagent LDR (8), Aspirating port InputPort =================== 17-03-18 00:58:24.838 -07 000000610 INF: LanePump: Xmit /1V50IP450R\r 17-03-18 00:58:24.878 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:27.368 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:27.368 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:58:29.528 -07 000000629 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.981281, 17-03-18 00:58:32.218 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:32.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:58:34.888 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.019446, 17-03-18 00:58:37.218 -07 000000629 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:37.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:58:40.248 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:58:41.298 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:41.328 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:41.748 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:41.778 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:42.198 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:42.218 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:42.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:58:42.228 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:42.648 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:42.678 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:43.098 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:43.128 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:43.548 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:43.578 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:58:43.998 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:44.028 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:44.028 -07 000000610 INF: LanePump: Waited 19149 ms for pump motion to complete 17-03-18 00:58:44.028 -07 000000610 INF: =================== Pumping Reagent LDR (8) =================== 17-03-18 00:58:44.158 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:58:44.188 -07 000000610 INF: LanePump: Recv /0`1170(0x03)\r\n 17-03-18 00:58:44.188 -07 000000610 INF: LanePump: Current Position of Pump is: 1170 17-03-18 00:58:44.318 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:58:44.348 -07 000000610 INF: LanePump: Recv /0`1170(0x03)\r\n 17-03-18 00:58:44.348 -07 000000610 INF: LanePump: Current Position of Pump is: 1170 17-03-18 00:58:44.348 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:58:44.478 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:58:44.508 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:44.938 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:58:44.968 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:58:44.968 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:58:44.968 -07 000000610 INF: =================== =================== 17-03-18 00:58:44.968 -07 000000610 INF: =================== Flow Cell Temp 65° =================== 17-03-18 00:58:44.968 -07 000000610 INF: =================== Flow Cell Temp 65°, FC temperature to 65° =================== 17-03-18 00:58:44.968 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 65000000\n [FlowCellTempControl] 17-03-18 00:58:44.968 -07 000000576 INF: FPGA: Recv TEC2TEMP 17-03-18 00:58:44.978 -07 000000610 INF: FPGA: 'TEC2TEMP 0 65000000' command took 1 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 00:58:44.978 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 00:58:44.978 -07 000000576 INF: FPGA: Recv TEC2EN 17-03-18 00:58:44.988 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 00:58:44.988 -07 000000610 INF: =================== Flow Cell Temp 65° =================== 17-03-18 00:58:44.988 -07 000000610 INF: WaitCommand(60000) start 17-03-18 00:58:45.608 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 00:58:47.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:47.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:58:50.968 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 00:58:52.218 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:52.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:58:56.328 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:58:57.328 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:58:57.328 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:02.218 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:02.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:02.678 -07 000000558 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.986717, 17-03-18 00:59:07.218 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:07.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:08.038 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.985358, 17-03-18 00:59:12.218 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:12.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:13.398 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.023489, 17-03-18 00:59:17.218 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:17.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:18.758 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.026244, 17-03-18 00:59:22.218 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:22.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:24.118 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.028963, 17-03-18 00:59:27.218 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:27.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:29.478 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.052111, 17-03-18 00:59:32.218 -07 000000579 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:32.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:34.838 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.019446, 17-03-18 00:59:37.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:37.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:40.198 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 00:59:42.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:42.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:44.998 -07 000000610 INF: WaitCommand(60000) end 17-03-18 00:59:44.998 -07 000000610 INF: =================== =================== 17-03-18 00:59:44.998 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 00:59:44.998 -07 000000610 INF: ReagentValve: Go to position 14 - PumpToFlowcell start 17-03-18 00:59:44.998 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:59:45.018 -07 000000610 INF: ReagentValve: Recv 08\r 17-03-18 00:59:45.018 -07 000000610 INF: ReagentValve: Taking shortest path '+' from 8 to 14. Moving 6 positions. 17-03-18 00:59:45.018 -07 000000610 INF: ReagentValve: Xmit +0E\r 17-03-18 00:59:45.538 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 00:59:45.558 -07 000000610 INF: ReagentValve: Recv 04C6\r 17-03-18 00:59:45.558 -07 000000610 INF: ReagentValve: Move+ from 8 to 14 took 1222 steps, expected 1225. Error = -3 steps (-0.2%) 17-03-18 00:59:45.558 -07 000000579 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 00:59:45.608 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 00:59:45.628 -07 000000610 INF: ReagentValve: Recv 0E\r 17-03-18 00:59:45.628 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 00:59:45.728 -07 000000610 INF: =================== Pumping Reagent HP11 (14) =================== 17-03-18 00:59:45.728 -07 000000610 INF: LanePump: Pumping from 14, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 300 µL, calling command Pumping to Flow Cell #1 17-03-18 00:59:45.728 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:59:45.758 -07 000000610 INF: LanePump: Recv /0`1170(0x03)\r\n 17-03-18 00:59:45.758 -07 000000610 INF: LanePump: Current Position of Pump is: 1170 17-03-18 00:59:45.758 -07 000000610 INF: =================== Pumping Reagent HP11 (14), Aspirating port InputPort =================== 17-03-18 00:59:45.888 -07 000000610 INF: LanePump: Xmit /1V400IP1800R\r 17-03-18 00:59:45.928 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:59:47.218 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:47.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:50.918 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.989471, 17-03-18 00:59:52.218 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:52.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 00:59:53.348 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:59:53.378 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:59:53.798 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:59:53.828 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:59:54.248 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:59:54.278 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:59:54.698 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:59:54.728 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 00:59:55.148 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:59:55.178 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:59:55.178 -07 000000610 INF: LanePump: Waited 9249 ms for pump motion to complete 17-03-18 00:59:55.178 -07 000000610 INF: =================== Pumping Reagent HP11 (14) =================== 17-03-18 00:59:55.308 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:59:55.338 -07 000000610 INF: LanePump: Recv /0`2970(0x03)\r\n 17-03-18 00:59:55.338 -07 000000610 INF: LanePump: Current Position of Pump is: 2970 17-03-18 00:59:55.468 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:59:55.498 -07 000000610 INF: LanePump: Recv /0`2970(0x03)\r\n 17-03-18 00:59:55.498 -07 000000610 INF: LanePump: Current Position of Pump is: 2970 17-03-18 00:59:55.498 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 00:59:55.628 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 00:59:55.658 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:59:56.088 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 00:59:56.118 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 00:59:56.118 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 00:59:56.118 -07 000000610 INF: =================== =================== 17-03-18 00:59:56.118 -07 000000610 INF: =================== Pumping Reagent HP11 (14) =================== 17-03-18 00:59:56.118 -07 000000610 INF: LanePump: Pumping from 14, aspirate port InputPort, aspiration rate 250 µL/min, dispense rate 7500 µL/min, volume 75 µL, calling command Pumping to Flow Cell #1 17-03-18 00:59:56.248 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:59:56.278 -07 000000610 INF: LanePump: Recv /0`2970(0x03)\r\n 17-03-18 00:59:56.278 -07 000000610 INF: LanePump: Current Position of Pump is: 2970 17-03-18 00:59:56.278 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 00:59:56.408 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 00:59:56.438 -07 000000610 INF: LanePump: Recv /0`2970(0x03)\r\n 17-03-18 00:59:56.438 -07 000000610 INF: LanePump: Current Position of Pump is: 2970 17-03-18 00:59:57.218 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 00:59:57.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:00.608 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:00.638 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:00:00.638 -07 000000610 INF: LanePump: Waited 4199 ms for pump motion to complete 17-03-18 01:00:00.768 -07 000000610 INF: LanePump: Xmit /1OR\r 17-03-18 01:00:00.798 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:01.228 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:01.258 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:00:01.258 -07 000000610 INF: LanePump: Waited 459 ms for pump motion to complete 17-03-18 01:00:01.388 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 01:00:01.418 -07 000000610 INF: LanePump: Recv /0`2970(0x03)\r\n 17-03-18 01:00:01.418 -07 000000610 INF: LanePump: Current Position of Pump is: 2970 17-03-18 01:00:01.548 -07 000000610 INF: LanePump: Xmit /1V500A0R\r 17-03-18 01:00:01.588 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:01.638 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.999021, 17-03-18 01:00:02.218 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:02.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:06.998 -07 000000479 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.008536, 17-03-18 01:00:07.218 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:07.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:11.888 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:11.918 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:12.218 -07 000000558 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:12.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:12.338 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:12.358 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.016692, 17-03-18 01:00:12.368 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:12.788 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:12.818 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:13.238 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:13.268 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:13.688 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:13.718 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:00:13.718 -07 000000610 INF: LanePump: Waited 12129 ms for pump motion to complete 17-03-18 01:00:13.718 -07 000000610 INF: =================== Pumping Reagent HP11 (14), Aspirating port InputPort =================== 17-03-18 01:00:13.848 -07 000000610 INF: LanePump: Xmit /1V50IP450R\r 17-03-18 01:00:13.888 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:17.218 -07 000000467 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:17.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:17.718 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.996267, 17-03-18 01:00:22.218 -07 000000462 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:22.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:23.078 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.96762, 17-03-18 01:00:27.218 -07 000000581 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:27.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:28.438 -07 000000581 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 01:00:30.308 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:30.338 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:30.758 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:30.788 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:31.208 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:31.238 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:31.658 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:31.688 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:32.108 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:32.138 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:32.218 -07 000000455 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:32.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:32.558 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:32.588 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:33.008 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:33.038 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:00:33.458 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:33.488 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:00:33.488 -07 000000610 INF: LanePump: Waited 19599 ms for pump motion to complete 17-03-18 01:00:33.488 -07 000000610 INF: =================== Pumping Reagent HP11 (14) =================== 17-03-18 01:00:33.618 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 01:00:33.648 -07 000000610 INF: LanePump: Recv /0`450(0x03)\r\n 17-03-18 01:00:33.648 -07 000000610 INF: LanePump: Current Position of Pump is: 450 17-03-18 01:00:33.778 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 01:00:33.798 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.993549, 17-03-18 01:00:33.808 -07 000000610 INF: LanePump: Recv /0`450(0x03)\r\n 17-03-18 01:00:33.808 -07 000000610 INF: LanePump: Current Position of Pump is: 450 17-03-18 01:00:33.808 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 01:00:33.938 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 01:00:33.968 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:00:34.398 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:00:34.428 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:00:34.428 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 01:00:34.428 -07 000000610 INF: =================== =================== 17-03-18 01:00:34.428 -07 000000610 INF: =================== Flow Cell Temp 40° =================== 17-03-18 01:00:34.428 -07 000000610 INF: =================== Flow Cell Temp 40°, FC temperature to 40° =================== 17-03-18 01:00:34.428 -07 000000610 INF: FPGA: Xmit TEC2TEMP 0 40000000\n [FlowCellTempControl] 17-03-18 01:00:34.428 -07 000000467 INF: FPGA: Recv TEC2TEMP 17-03-18 01:00:34.438 -07 000000610 INF: FPGA: 'TEC2TEMP 0 40000000' command took 1 ms to send, 0 ms to get response, and 6 ms overall 17-03-18 01:00:34.438 -07 000000610 INF: FPGA: Xmit TEC2EN 0 1\n [FlowCellTempControl] 17-03-18 01:00:34.438 -07 000000576 INF: FPGA: Recv TEC2EN 17-03-18 01:00:34.448 -07 000000610 INF: FPGA: 'TEC2EN 0 1' command took 0 ms to send, 0 ms to get response, and 9 ms overall 17-03-18 01:00:34.448 -07 000000610 INF: =================== Flow Cell Temp 40° =================== 17-03-18 01:00:34.448 -07 000000610 INF: WaitCommand(60000) start 17-03-18 01:00:37.218 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:37.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:39.158 -07 000000462 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.015368, 17-03-18 01:00:42.218 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:42.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:44.518 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.014009, 17-03-18 01:00:47.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:47.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:49.878 -07 000000581 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.052111, 17-03-18 01:00:52.218 -07 000000591 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:52.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:00:55.238 -07 000000591 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.041198, 17-03-18 01:00:57.218 -07 000000455 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:00:57.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:00.598 -07 000000576 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.02213, 17-03-18 01:01:02.218 -07 000000474 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:02.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:05.958 -07 000000455 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.974486, 17-03-18 01:01:07.218 -07 000000576 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:07.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:11.318 -07 000000467 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.978563, 17-03-18 01:01:12.218 -07 000000430 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:12.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:16.678 -07 000000430 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 01:01:17.218 -07 000000430 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:17.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:22.038 -07 000000581 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 01:01:22.218 -07 000000581 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:22.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:27.218 -07 000000430 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:27.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:27.398 -07 000000455 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.960826, 17-03-18 01:01:32.218 -07 000000430 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:32.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:32.758 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 3.990831, 17-03-18 01:01:34.458 -07 000000610 INF: WaitCommand(60000) end 17-03-18 01:01:34.458 -07 000000610 INF: =================== =================== 17-03-18 01:01:34.458 -07 000000610 INF: AsyncDelayTimer did not wait because wait time had elapsed 17-03-18 01:01:34.458 -07 000000610 INF: ReagentValve: Go to position 3 - PumpToFlowcell start 17-03-18 01:01:34.458 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 01:01:34.488 -07 000000610 INF: ReagentValve: Recv 0E\r 17-03-18 01:01:34.488 -07 000000610 INF: ReagentValve: Taking shortest path '-' from 14 to 3. Moving 11 positions. 17-03-18 01:01:34.488 -07 000000610 INF: ReagentValve: Xmit -03\r 17-03-18 01:01:35.408 -07 000000610 INF: ReagentValve: Xmit B\r 17-03-18 01:01:35.428 -07 000000610 INF: ReagentValve: Recv 08D9\r 17-03-18 01:01:35.428 -07 000000610 INF: ReagentValve: Move- from 14 to 3 took 2265 steps, expected 2246. Error = 19 steps (0.8%) 17-03-18 01:01:35.478 -07 000000610 INF: ReagentValve: Xmit S\r 17-03-18 01:01:35.498 -07 000000610 INF: ReagentValve: Recv 03\r 17-03-18 01:01:35.498 -07 000000610 INF: LanePump: Waiting 100 ms after setting reagent valve before aspirating 17-03-18 01:01:35.598 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 01:01:35.598 -07 000000610 INF: LanePump: Pumping from 3, aspirate port InputPort, aspiration rate 2000 µL/min, dispense rate 7500 µL/min, volume 120 µL, calling command Pumping to Flow Cell #1 17-03-18 01:01:35.598 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 01:01:35.628 -07 000000610 INF: LanePump: Recv /0`450(0x03)\r\n 17-03-18 01:01:35.628 -07 000000610 INF: LanePump: Current Position of Pump is: 450 17-03-18 01:01:35.628 -07 000000610 INF: =================== Pumping Reagent PR2 (3), Aspirating port InputPort =================== 17-03-18 01:01:35.758 -07 000000610 INF: LanePump: Xmit /1V400IP720R\r 17-03-18 01:01:35.798 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:01:37.218 -07 000000430 INF: FPGA: Recv @BUBEN2 C2 0 C1 0 R2 0 R1 0 17-03-18 01:01:37.218 -07 000000005 INF: Predictive Diagnostics Bubble Status: CyclesCompleted: 82. Bubbles during last 5000 ms interval: R1=0, R2=0, Total=0. Max bubbles per interval: R1=25, R2=22, Total=47. Bubbles this run: R1=1375, R2=1427, Total=2802. Warn if over 250 bubbles per interval. 17-03-18 01:01:38.118 -07 000000474 INF: Predictive Diagnostic Log Reagent Chiller Temperature Below Limit: RunId: 170317_M03814_0172_000000000-B2K79, InstrumentID: M03814, TotalCycles 158, CycleNumber: 82, FrontChillerTemperature: 4.012614, 17-03-18 01:01:38.928 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:01:38.958 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:01:39.378 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:01:39.408 -07 000000610 INF: LanePump: Recv /0@(0x03)\r\n 17-03-18 01:01:39.828 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:01:39.858 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:01:39.858 -07 000000610 INF: LanePump: Waited 4059 ms for pump motion to complete 17-03-18 01:01:39.858 -07 000000610 INF: =================== Pumping Reagent PR2 (3) =================== 17-03-18 01:01:39.988 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 01:01:40.018 -07 000000610 INF: LanePump: Recv /0`1170(0x03)\r\n 17-03-18 01:01:40.018 -07 000000610 INF: LanePump: Current Position of Pump is: 1170 17-03-18 01:01:40.148 -07 000000610 INF: LanePump: Xmit /1?\r 17-03-18 01:01:40.178 -07 000000610 INF: LanePump: Recv /0`1170(0x03)\r\n 17-03-18 01:01:40.178 -07 000000610 INF: LanePump: Current Position of Pump is: 1170 17-03-18 01:01:40.178 -07 000000610 INF: LanePump: Waiting 2 ms before setting solenoid valve back to 'aspirate' position after PumpSolution 17-03-18 01:01:40.308 -07 000000610 INF: LanePump: Xmit /1IR\r 17-03-18 01:01:40.338 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:01:40.768 -07 000000610 INF: LanePump: Xmit /1Q\r 17-03-18 01:01:40.798 -07 000000610 INF: LanePump: Recv /0`(0x03)\r\n 17-03-18 01:01:40.798 -07 000000610 INF: LanePump: Waited 460 ms for pump motion to complete 17-03-18 01:01:40.798 -07 000000610 INF: =================== =================== 17-03-18 01:01:40.798 -07 000000610 INF: =================== FC temp control off =================== 17-03-18 01:01:40.798 -07 000000610 INF: FPGA: Xmit TEC2EN 0 0\n [FlowCellTempControl] 17-03-18 01:01:40.798 -07 000000474 INF: FPGA: Recv TEC2EN 17-03-18 01:01:40.808 -07 000000610 INF: FPGA: 'TEC2EN 0 0' command took 0 ms to send, 0 ms to get response, and 7 ms overall 17-03-18 01:01:40.808 -07 000000610 INF: =================== =================== 17-03-18 01:01:40.938 -07 000000610 INF: Y-Drift Offset assigned 0.002126916.