FastQCFastQC Report
Thu 2 Mar 2017
160123_I132_FCH3YHMBBXX_L4_OYSzenG1AAD96FAAPEI-109_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160123_I132_FCH3YHMBBXX_L4_OYSzenG1AAD96FAAPEI-109_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364261046
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG4519550.087.170391
TAGCGGA4625550.085.596411
CCGGATA3690600.083.878971
CCGAGAC5290250.082.641773
TCGGGAC4220450.082.398193
CGCCTTA3091800.082.330671
AACCGAG5318950.082.04241
GGTACGT5375650.081.749822
GGACCTA5841900.081.638271
ACCTAAC5228600.081.258281
GTCGATT5131350.081.1358261
CTACGGA4003250.081.0122451
GGTAGAC4392600.080.814282
CGTGTGG5601150.080.5201641
GTACGTC5459800.080.477133
TCGAAGA5356800.080.2984541
CGAAGAC5368500.080.2735442
CGGACAG7380000.080.251224
TGGTACG5484200.080.008351
GCGGAGA4961950.079.7083362
Produced by FastQC (version 0.11.5)