FastQCFastQC Report
Thu 2 Mar 2017
160123_I132_FCH3YHMBBXX_L4_OYSzenG1AAD96FAAPEI-109_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160123_I132_FCH3YHMBBXX_L4_OYSzenG1AAD96FAAPEI-109_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364261046
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGATTCGGCCCAAATTTGCACCACATCAGGCCCTTGACAGGGCGCTT25882840.7105574500546512No Hit
CAGCGAATCGCACCAAATTTGCACCATCGAAGCGCCCTGTCAAGGGCCTG15961700.43819398684755323No Hit
CAGCGAATCGCGCCAAATTTGCACCATCGAAGCGCCCTGTCAAGGGCCTG4829960.13259611624790646No Hit
NAGCGATTCGGCCCAAATTTGCACCACATCAGGCCCTTGACAGGGCGCTT4183520.11484950273820933No Hit
GAGCGATTCGGCCCAAATTTGCACCACATCAGGCCCTTGACAGGGCGCTT4021450.11040022105465541No Hit
CAGCAAATCGCACCAAATTTGCACCATCGAAGCGCCCTGTCAAGGGCCTG3779020.1037448291959278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC7212050.089.5304958
CAGCGAA3729050.086.604821
TTCGGCC7494850.086.5054867
CGGCCCA7504100.086.093069
ATTCGGC7650350.084.803176
TCGCACC6762900.084.488878
GATTCGG8349300.083.645955
ATCGCAC6865800.083.3064657
AGCGAAT5000600.083.0646062
CAGCGAT4565400.082.956351
ATCGCGC3263300.082.847797
CAGCTGC203450.079.395351
GCGAATC5976600.079.239553
TCACCGG11008400.079.187788
AATCGCG3418200.079.187116
AGCGATT5321700.078.7431952
TCGCGCC3432400.078.599128
GTGTGGC9869800.078.597777
CGCGCCA3480900.077.3137749
AATCGCA7415450.077.305476