FastQCFastQC Report
Tue 7 Mar 2017
1HL_10A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_10A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3186565
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA148090.4647323999353536No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG95890.30091964231076407No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG76460.2399448936393891No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC59410.18643900249955672No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG57500.18044508742172213No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA57480.18038232391305373No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT56860.17843665514433255No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG53040.16644882498866334No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG49920.15665771763638903No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA48400.15188769097758872No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA47110.14783944466847532No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT47070.14771391765113845No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC46620.1463017387060989No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA44230.1388014994202221No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA43020.13500430714578238No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA40330.1265626152298792No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT38800.12176120681674468No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA36020.11303707911183358No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA33800.10607032964963839No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC33570.10534854929995152No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT33250.10434433316125671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCG202.6993124E-586.999682
CAGCAGC6800.086.999671
GCTGCTA450.086.999673
GCTGCAC302.2702807E-886.999673
GCTGCAA850.086.999673
TGCTGCC302.2702807E-886.999672
GCAGCTT550.086.999673
GCAGCTA356.6393113E-1086.999673
GCAGCCT356.6393113E-1086.999673
GCAGCCA550.086.999673
AGCAGCT1450.086.999672
AGCAGCC1200.086.999672
CAGCTGC5900.084.050531
GCTGCCC1250.083.519693
AGCAGCG1250.083.519692
AGCAGCA3100.082.790012
AGCTGCA1850.082.296992
GCAGCAA900.082.166363
AGCTGCT1700.081.882042
GCAGCAC800.081.5621953
Produced by FastQC (version 0.11.5)