FastQCFastQC Report
Tue 7 Mar 2017
1HL_10A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_10A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3186565
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG127300.082.335768
CACCGGC126700.079.576689
CTTCACC144400.075.641026
CCTTCAC161350.071.593055
TTCACCG152150.070.584977
CGTAGTG30250.069.5153664
CGGGCCC10550.068.961627
CAGCTGC1100.067.428361
GCGTAGT19500.065.954453
GGGCCCG11250.064.253448
GTGTGGC47600.064.195157
AACGGGC11200.062.863925
CAGCGTA13150.062.044351
GGCCCGA11700.061.7821589
GCCTTCA48500.060.874744
ACGGGCC11600.060.6961946
GCAGCCT351.6258385E-460.547921
ACCTTCA63000.060.274854
CGGGGCA12950.058.35583594
TGTGGCA55850.057.7388
Produced by FastQC (version 0.11.5)