##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_10A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3186565 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.809376240559976 12.0 12.0 12.0 2.0 32.0 2 14.672066943558345 12.0 12.0 12.0 12.0 22.0 3 13.70516998711779 12.0 12.0 12.0 12.0 22.0 4 13.752595663355368 12.0 12.0 12.0 12.0 22.0 5 22.450663959467327 22.0 22.0 22.0 22.0 27.0 6 26.81097765148365 27.0 22.0 32.0 22.0 32.0 7 35.00511114632841 37.0 37.0 37.0 27.0 41.0 8 37.623669060571494 41.0 37.0 41.0 32.0 41.0 9 38.553512010581926 41.0 41.0 41.0 32.0 41.0 10-11 38.90843290502469 41.0 41.0 41.0 37.0 41.0 12-13 39.09323958557255 41.0 41.0 41.0 37.0 41.0 14-15 39.04777197389666 41.0 41.0 41.0 37.0 41.0 16-17 38.955475096224305 41.0 41.0 41.0 37.0 41.0 18-19 38.89731168201496 41.0 41.0 41.0 37.0 41.0 20-21 38.90659770003123 41.0 41.0 41.0 37.0 41.0 22-23 38.890413344777215 41.0 41.0 41.0 37.0 41.0 24-25 38.91642395494836 41.0 41.0 41.0 37.0 41.0 26-27 38.88125301068706 41.0 41.0 41.0 37.0 41.0 28-29 38.831672820105666 41.0 41.0 41.0 37.0 41.0 30-31 38.708309731638934 41.0 41.0 41.0 32.0 41.0 32-33 38.74875077081434 41.0 41.0 41.0 32.0 41.0 34-35 38.72203469880577 41.0 41.0 41.0 32.0 41.0 36-37 38.646082537152076 41.0 41.0 41.0 32.0 41.0 38-39 38.55407437161959 41.0 41.0 41.0 32.0 41.0 40-41 38.59821641799242 41.0 41.0 41.0 32.0 41.0 42-43 38.3354113284995 41.0 41.0 41.0 32.0 41.0 44-45 38.44720553323092 41.0 41.0 41.0 32.0 41.0 46-47 38.45744759639298 41.0 41.0 41.0 32.0 41.0 48-49 38.40156830317285 41.0 41.0 41.0 32.0 41.0 50-51 38.277217944714764 41.0 41.0 41.0 32.0 41.0 52-53 38.26000128665193 41.0 41.0 41.0 32.0 41.0 54-55 38.12569428208745 41.0 37.0 41.0 32.0 41.0 56-57 37.91113440334655 41.0 37.0 41.0 29.5 41.0 58-59 37.920017165819615 41.0 37.0 41.0 29.5 41.0 60-61 37.924016770409516 41.0 37.0 41.0 29.5 41.0 62-63 37.919715116434155 41.0 37.0 41.0 29.5 41.0 64-65 37.898863980493104 41.0 37.0 41.0 29.5 41.0 66-67 37.76319940123612 41.0 37.0 41.0 27.0 41.0 68-69 37.786149348907045 41.0 37.0 41.0 27.0 41.0 70-71 37.775834950801254 41.0 37.0 41.0 27.0 41.0 72-73 37.62406509831119 41.0 37.0 41.0 27.0 41.0 74-75 37.539284621528196 41.0 37.0 41.0 27.0 41.0 76-77 36.498464647669195 39.0 34.5 41.0 24.5 41.0 78-79 37.01741106803094 41.0 37.0 41.0 27.0 41.0 80-81 37.66198069080656 41.0 37.0 41.0 27.0 41.0 82-83 37.863841158112265 41.0 37.0 41.0 32.0 41.0 84-85 37.770571602964324 41.0 37.0 41.0 27.0 41.0 86-87 37.63853977558908 41.0 37.0 41.0 27.0 41.0 88-89 37.55384387263401 41.0 37.0 41.0 27.0 41.0 90-91 37.40841784178261 41.0 37.0 41.0 27.0 41.0 92-93 37.287830940213055 41.0 37.0 41.0 27.0 41.0 94-95 37.12692209322578 41.0 37.0 41.0 27.0 41.0 96-97 37.07557856186834 41.0 37.0 41.0 27.0 41.0 98-99 36.96471325706521 41.0 37.0 41.0 27.0 41.0 100 36.83918733808976 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1569128190606754 1101 2 -0.6927846590513269 1101 3 -0.024444811179952453 1101 4 0.015057150606876846 1101 5 -1.123811856281499 1101 6 -0.8030894423330643 1101 7 -0.20591447711522193 1101 8 -0.061544004230874805 1101 9 -0.24039387399887602 1101 10-11 -0.17955258478082925 1101 12-13 -0.1729141419855651 1101 14-15 -0.10897031004881796 1101 16-17 -0.17601147292607067 1101 18-19 -0.06935142228509505 1101 20-21 -0.1435325250140167 1101 22-23 -0.07612289643958547 1101 24-25 -0.045017713912386625 1101 26-27 -0.007588071248505912 1101 28-29 -0.04846680175522522 1101 30-31 -0.11034837818356635 1101 32-33 -0.21546667411905673 1101 34-35 -0.048575801547436015 1101 36-37 0.10768670521594004 1101 38-39 -0.031137567193283644 1101 40-41 0.025292884863993947 1101 42-43 0.07124587002759597 1101 44-45 -0.007072249220485105 1101 46-47 0.10516156032833379 1101 48-49 -0.0218259755606951 1101 50-51 0.02804886773788695 1101 52-53 -0.0855611680904147 1101 54-55 -0.041645622745996036 1101 56-57 0.09829079409742292 1101 58-59 0.1752487220201644 1101 60-61 0.038317170883637175 1101 62-63 -0.16895290088368142 1101 64-65 -0.055619280266355986 1101 66-67 -0.06750854967136632 1101 68-69 -0.050160500595929136 1101 70-71 -0.09402752774447265 1101 72-73 -0.0841318198560046 1101 74-75 0.08186719358760541 1101 76-77 -0.4056495220475256 1101 78-79 -0.2629146738314603 1101 80-81 -0.1924806695633876 1101 82-83 -0.28900709744324615 1101 84-85 -0.22776769126025442 1101 86-87 -0.2834175515407509 1101 88-89 -0.3767725436039484 1101 90-91 -0.08653298781552365 1101 92-93 -0.10787858481494794 1101 94-95 -0.07126124154723357 1101 96-97 -0.25017529514821035 1101 98-99 -0.5321184602607403 1101 100 -0.7363066305662542 1102 1 1.6767706481949851 1102 2 0.8217635255202111 1102 3 0.038064403458603735 1102 4 -0.021288780384852046 1102 5 -0.8429100469667752 1102 6 -0.11996759072032148 1102 7 0.08508003460831048 1102 8 -0.1617310896979376 1102 9 -0.15048353211754772 1102 10-11 -0.2502951575934631 1102 12-13 -0.27389890694875874 1102 14-15 -0.22019930004392307 1102 16-17 -0.25162585344824606 1102 18-19 -0.22988482972131408 1102 20-21 -0.096674484037365 1102 22-23 -0.05431647001857698 1102 24-25 -0.09368553506109834 1102 26-27 -0.1236525135873805 1102 28-29 -0.25351483864095314 1102 30-31 -0.40702520841835366 1102 32-33 -0.30599656678392506 1102 34-35 -0.2242644904245097 1102 36-37 -0.4204476305023448 1102 38-39 -0.3093381979903356 1102 40-41 -0.28712267382363166 1102 42-43 -0.21686398808835605 1102 44-45 -0.18732196403843204 1102 46-47 -0.5101193546896781 1102 48-49 -0.4134942188845159 1102 50-51 -0.3024189541152893 1102 52-53 -0.2378944906890652 1102 54-55 -0.2971021983372282 1102 56-57 -0.29088729536646696 1102 58-59 -0.16617873304888064 1102 60-61 -0.24358843649752515 1102 62-63 -0.23377338588690577 1102 64-65 -0.33134265140758856 1102 66-67 -0.25656733315290126 1102 68-69 -0.5153516216343874 1102 70-71 -0.519840567068151 1102 72-73 -0.18730998328859982 1102 74-75 -0.44453843819821515 1102 76-77 -0.32153616274631247 1102 78-79 -0.3823775251890851 1102 80-81 -0.4559155496812579 1102 82-83 -0.43280797923069514 1102 84-85 -0.4389551933673559 1102 86-87 -0.31422683707856436 1102 88-89 -0.2629894319167434 1102 90-91 -0.4515876567250743 1102 92-93 -0.3381454206200232 1102 94-95 -0.14423916616658516 1102 96-97 -0.11505170954805521 1102 98-99 -0.38919079913602417 1102 100 -0.6199811592170477 1103 1 1.4822757448864596 1103 2 0.9280581952466065 1103 3 0.37302081582817515 1103 4 0.9848507997385436 1103 5 2.929917179632298 1103 6 -0.17847464362319343 1103 7 -0.44775570335387727 1103 8 -0.15671566901901457 1103 9 -0.22521319637309034 1103 10-11 -0.2093113733240628 1103 12-13 -0.40714295925079114 1103 14-15 -0.3333394114134691 1103 16-17 -0.4350576724444295 1103 18-19 -0.25746412586949674 1103 20-21 -0.18838396717068662 1103 22-23 -0.28991628483540666 1103 24-25 -0.368626061976272 1103 26-27 -0.13133007209846426 1103 28-29 -0.20136553769961552 1103 30-31 -0.12917191544518403 1103 32-33 -0.35266249084844503 1103 34-35 -0.36561160448041363 1103 36-37 -0.18426918320554364 1103 38-39 -0.0997172548440588 1103 40-41 -0.39332750426886065 1103 42-43 -0.04618201187344084 1103 44-45 -0.3605923371726405 1103 46-47 -0.49964911875510865 1103 48-49 -0.43142916433961176 1103 50-51 -0.19707369864197233 1103 52-53 -0.22959591165568582 1103 54-55 -0.259059287357573 1103 56-57 -0.40994907467238306 1103 58-59 -0.4592332348537127 1103 60-61 -0.15072350596198447 1103 62-63 -0.18145102748633235 1103 64-65 -0.3029895949765802 1103 66-67 -0.3929518006162809 1103 68-69 -0.22149064350792003 1103 70-71 -0.4878207826928147 1103 72-73 -0.26446504848438934 1103 74-75 -0.44422692178904555 1103 76-77 -0.49545326186188987 1103 78-79 -0.43829494782993805 1103 80-81 -0.1914816098548755 1103 82-83 -0.32740969001986997 1103 84-85 -0.3537585388160096 1103 86-87 -0.3819579953665482 1103 88-89 -0.2990830122509749 1103 90-91 -0.613399302180845 1103 92-93 -0.1616150629921762 1103 94-95 -0.2851085221955003 1103 96-97 -0.2253073568296884 1103 98-99 -0.34919635047657493 1103 100 -0.28501085551354066 1104 1 1.5883870117004086 1104 2 -0.6056665176121339 1104 3 0.10952333690938332 1104 4 0.17202392163612146 1104 5 -1.1371620821587989 1104 6 -0.5070329014493495 1104 7 -0.19603901432341075 1104 8 -0.19102178845370332 1104 9 -0.0015021418717040547 1104 10-11 -0.11724826461512095 1104 12-13 -0.06476018130688033 1104 14-15 -0.2076579940340082 1104 16-17 -0.15256453424241556 1104 18-19 -0.1482363449689572 1104 20-21 0.13161361378130465 1104 22-23 -0.03169184780554701 1104 24-25 -0.11270839728920379 1104 26-27 -0.08688711074529465 1104 28-29 -0.10477278116962907 1104 30-31 -0.12391903228964196 1104 32-33 -0.15502900871886283 1104 34-35 -0.07317355637815126 1104 36-37 -0.12551782689464375 1104 38-39 -0.1174444796506009 1104 40-41 -0.08427650697914402 1104 42-43 -0.08216939576826832 1104 44-45 -0.11506444453140574 1104 46-47 -0.3755986016216255 1104 48-49 -0.31751972963485997 1104 50-51 -0.07886640893083552 1104 52-53 -0.12416025598407288 1104 54-55 -0.17466934934470402 1104 56-57 -0.14061541410212897 1104 58-59 -0.10720283939807729 1104 60-61 -0.26587599732047806 1104 62-63 -0.0933584716303244 1104 64-65 -0.22920243310182542 1104 66-67 -0.5522189725951989 1104 68-69 -0.44536290263780387 1104 70-71 -0.19801870100675245 1104 72-73 -0.15446542947792352 1104 74-75 -0.24729612482884278 1104 76-77 -0.44019347646040785 1104 78-79 -0.27983347280041215 1104 80-81 -0.2998687259060304 1104 82-83 -0.24886532801964734 1104 84-85 -0.2448048905941249 1104 86-87 -0.30479858401722026 1104 88-89 -0.24491903993929043 1104 90-91 -0.3191206116712877 1104 92-93 -0.4531917003640942 1104 94-95 -0.2736438134180901 1104 96-97 -0.3153956272912666 1104 98-99 -0.4067619948801706 1104 100 -0.13345306166444715 1105 1 1.5430354721866166 1105 2 0.3701153502988195 1105 3 -0.12444374753910559 1105 4 -0.24324493724589935 1105 5 -1.21315335870635 1105 6 0.3133943193342077 1105 7 -0.1138985363545757 1105 8 -0.2257887029706822 1105 9 -0.12168252683095915 1105 10-11 -0.17859734872430266 1105 12-13 -0.1876840319457358 1105 14-15 -0.20015444656291237 1105 16-17 -0.2663709785853854 1105 18-19 -0.2925306670407295 1105 20-21 -0.3242734275454282 1105 22-23 -0.16525006286573785 1105 24-25 -0.14550623425934361 1105 26-27 -0.2064770365436317 1105 28-29 -0.16613689400504938 1105 30-31 -0.1885051405399807 1105 32-33 -0.2040105201157303 1105 34-35 -0.22922128560049515 1105 36-37 -0.3432777820423851 1105 38-39 -0.09202587303109055 1105 40-41 -0.1073717605552531 1105 42-43 -0.07031977961702296 1105 44-45 -0.2569781912129727 1105 46-47 -0.4554659061434805 1105 48-49 -0.30612078662592523 1105 50-51 -0.28128417255828 1105 52-53 -0.27374230019015755 1105 54-55 -0.40068315640528596 1105 56-57 -0.2900153529056553 1105 58-59 -0.384152898374829 1105 60-61 -0.4570303280020127 1105 62-63 -0.4996356247343172 1105 64-65 -0.43638637804561853 1105 66-67 -0.28892499524091164 1105 68-69 -0.3586279103930039 1105 70-71 -0.4266135503164108 1105 72-73 -0.3502093696628421 1105 74-75 -0.47761512746472334 1105 76-77 -0.4216040110818824 1105 78-79 -0.42774449871659925 1105 80-81 -0.39130915574521197 1105 82-83 -0.42969555258654424 1105 84-85 -0.40646757831643754 1105 86-87 -0.48863128398511435 1105 88-89 -0.3923705892752025 1105 90-91 -0.25380312637083335 1105 92-93 -0.320620853753816 1105 94-95 -0.23792411538607894 1105 96-97 -0.33897211451577647 1105 98-99 -0.5620802335033943 1105 100 -0.5119611530929333 1106 1 1.259144852019224 1106 2 -0.6877119689937992 1106 3 -0.15332974189882265 1106 4 -0.18624033818337793 1106 5 -1.0780706959341728 1106 6 -0.5540002572145362 1106 7 -0.025040948294005716 1106 8 -0.018755561429557588 1106 9 -0.19771556764835907 1106 10-11 0.014387900095819361 1106 12-13 2.698152405429255E-4 1106 14-15 -0.11453676413857039 1106 16-17 -0.0526500147145299 1106 18-19 0.07044028538982872 1106 20-21 0.1304042080699901 1106 22-23 0.0800311041652364 1106 24-25 -0.20378730105733212 1106 26-27 -0.0880298602492644 1106 28-29 -0.00810259420241266 1106 30-31 0.07956713124870163 1106 32-33 0.05927308072435977 1106 34-35 6.146770392163603E-4 1106 36-37 -0.2961863115262844 1106 38-39 -0.06074299749997181 1106 40-41 0.02803932781430518 1106 42-43 0.135303597807777 1106 44-45 0.028800845468246905 1106 46-47 -0.11372674231726165 1106 48-49 -0.09124567116476356 1106 50-51 -0.11160125354788164 1106 52-53 -0.03426354363376305 1106 54-55 -0.015661437008333223 1106 56-57 -0.11825109648047061 1106 58-59 -0.20463101341555756 1106 60-61 -0.2094366251855817 1106 62-63 -0.27468586698039843 1106 64-65 -0.2743214116101811 1106 66-67 -0.32379724234553464 1106 68-69 -0.20065912395195795 1106 70-71 -0.4510209580373541 1106 72-73 -0.17188985118359312 1106 74-75 -0.28169662216065916 1106 76-77 -0.42603389209416065 1106 78-79 -0.20223812275750674 1106 80-81 -0.34883557977442337 1106 82-83 -0.16560413764638326 1106 84-85 -0.3378501838319039 1106 86-87 -0.40802942230148886 1106 88-89 -0.24675763509983284 1106 90-91 -0.3346944614190406 1106 92-93 -0.46302954205240354 1106 94-95 -0.41788790882563376 1106 96-97 -0.2201455373398744 1106 98-99 -0.4320294631018413 1106 100 -0.3760872999294733 1107 1 1.3118427285559484 1107 2 -0.6367834793399538 1107 3 -0.1364095522553761 1107 4 -0.21173132594033994 1107 5 -1.2096794958756618 1107 6 -0.22739681332219774 1107 7 -0.4314976618142907 1107 8 -0.3797450647673273 1107 9 -0.39569745907753173 1107 10-11 -0.32384753669956723 1107 12-13 -0.4194382066931368 1107 14-15 -0.4489967833337758 1107 16-17 -0.4979944313131597 1107 18-19 -0.45869905995319726 1107 20-21 -0.4182239542503936 1107 22-23 -0.19974932518442046 1107 24-25 -0.2424613716596582 1107 26-27 -0.410734620291791 1107 28-29 -0.21885659805438706 1107 30-31 -0.3857324865886085 1107 32-33 -0.15321859165160845 1107 34-35 -0.2937634117691985 1107 36-37 -0.4149236770466658 1107 38-39 -0.3390507621044776 1107 40-41 -0.4287387486550571 1107 42-43 -0.39932103345074665 1107 44-45 -0.32450790178344846 1107 46-47 -0.5310908397222249 1107 48-49 -0.4399130274258667 1107 50-51 -0.42694629705054155 1107 52-53 -0.3901461424196597 1107 54-55 -0.5463449066932924 1107 56-57 -0.3598893907641525 1107 58-59 -0.4946778670289831 1107 60-61 -0.3849484226736877 1107 62-63 -0.5053532162759069 1107 64-65 -0.6661785998858747 1107 66-67 -0.5534924550376488 1107 68-69 -0.6730250324130296 1107 70-71 -0.5690188504456941 1107 72-73 -0.5493027475946661 1107 74-75 -0.5006240739400383 1107 76-77 -0.5918593381289128 1107 78-79 -0.7683244429651523 1107 80-81 -0.782638556274982 1107 82-83 -0.551181899370107 1107 84-85 -0.5418653424709561 1107 86-87 -0.39148904445747235 1107 88-89 -0.4239090851637002 1107 90-91 -0.35526717557020504 1107 92-93 -0.41671744185921256 1107 94-95 -0.3518269011122257 1107 96-97 -0.32593789428509723 1107 98-99 -0.5627821783177467 1107 100 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2223 8 0.22553690107941549 2223 9 0.4125161220456164 2223 10-11 0.2164805482252632 2223 12-13 0.22323198794936872 2223 14-15 0.20439765604572102 2223 16-17 0.2017113727218458 2223 18-19 0.20287560118195103 2223 20-21 0.10190686495470658 2223 22-23 0.3117834140156077 2223 24-25 0.28138624532332557 2223 26-27 0.25249699666743197 2223 28-29 0.3175470592013738 2223 30-31 0.13500427038592733 2223 32-33 0.12174364396055637 2223 34-35 0.35577886207260434 2223 36-37 0.20933155280136617 2223 38-39 0.20729858966460313 2223 40-41 0.29377763653575784 2223 42-43 0.29655463014823624 2223 44-45 0.3494271721763482 2223 46-47 0.19696720953678692 2223 48-49 0.2902841789003361 2223 50-51 0.1981364826855767 2223 52-53 0.27809767001160424 2223 54-55 0.20528297104709736 2223 56-57 0.39555798607098325 2223 58-59 0.4397128196691398 2223 60-61 0.3751077685576476 2223 62-63 0.3988734749796876 2223 64-65 0.42008134976820344 2223 66-67 0.5807212141859068 2223 68-69 0.39183109665307114 2223 70-71 0.46813427895005333 2223 72-73 0.5197348099056924 2223 74-75 0.4466852010530076 2223 76-77 0.5177098508820919 2223 78-79 0.5659108059085938 2223 80-81 0.5055434423371921 2223 82-83 0.47567845455594693 2223 84-85 0.3862896419827848 2223 86-87 0.4901281374975852 2223 88-89 0.3914051165228827 2223 90-91 0.5549489822423723 2223 92-93 0.5076729825174837 2223 94-95 0.791069483900408 2223 96-97 0.6110617770706881 2223 98-99 0.7009133516259993 2223 100 0.7283683946832227 2224 1 1.2402454864651915 2224 2 -0.9074667912488508 2224 3 -0.027958418103480653 2224 4 -0.20408753707916638 2224 5 -1.0275759856261857 2224 6 0.5874643240937338 2224 7 0.36148284665004127 2224 8 0.3444791221450956 2224 9 0.3915619189825037 2224 10-11 0.2753416424154338 2224 12-13 0.15565780030237875 2224 14-15 0.12852005063154337 2224 16-17 0.25946726663050157 2224 18-19 0.16359022836598314 2224 20-21 0.19677691212962856 2224 22-23 0.2847769765011918 2224 24-25 0.3267578587530977 2224 26-27 0.1751052545465157 2224 28-29 0.11962170617798051 2224 30-31 -0.016665678614693036 2224 32-33 0.22319154257684914 2224 34-35 0.15185487126708352 2224 36-37 0.07155349081749307 2224 38-39 0.04661528264908554 2224 40-41 0.17503846675925416 2224 42-43 0.27800328281422537 2224 44-45 0.13941694075702316 2224 46-47 0.2702391543541509 2224 48-49 0.1530747344526091 2224 50-51 0.13145779065128949 2224 52-53 0.03944008052611281 2224 54-55 0.14422293489213445 2224 56-57 0.23109839327753434 2224 58-59 0.3367512771890375 2224 60-61 0.36494202055020963 2224 62-63 0.3149426927985246 2224 64-65 0.32276372136783493 2224 66-67 0.3335915314387634 2224 68-69 0.3479458975149612 2224 70-71 0.414882599834165 2224 72-73 0.37389568566787545 2224 74-75 0.34099158376328376 2224 76-77 0.45472193637654357 2224 78-79 0.29910510824998937 2224 80-81 0.283435765027086 2224 82-83 0.3644568644869395 2224 84-85 0.15179275576444695 2224 86-87 0.4067564856260617 2224 88-89 0.17633511311241534 2224 90-91 0.16609085496666864 2224 92-93 0.19418323069346854 2224 94-95 0.392982626247246 2224 96-97 0.17288695188207726 2224 98-99 0.23687474526753505 2224 100 0.4422035801203066 2225 1 1.4315166897252034 2225 2 1.7975330260814673 2225 3 0.8433790547552604 2225 4 0.9794620458908838 2225 5 3.17999861025417 2225 6 0.03176920176922593 2225 7 0.26858617943371854 2225 8 0.4668019004621087 2225 9 0.18100079721291706 2225 10-11 0.23776753776841275 2225 12-13 0.291380140409629 2225 14-15 0.23201081890073993 2225 16-17 0.22480661999956908 2225 18-19 0.16870195556214185 2225 20-21 0.16499201703633304 2225 22-23 0.22881840252935604 2225 24-25 0.25331477220815657 2225 26-27 0.153906746414755 2225 28-29 0.2747293109637283 2225 30-31 0.21069907917024722 2225 32-33 0.25569236705892706 2225 34-35 0.16820893003932014 2225 36-37 0.2791264204541122 2225 38-39 0.09671059179036945 2225 40-41 0.15973025363408766 2225 42-43 0.2840628558411211 2225 44-45 0.19674090889547813 2225 46-47 0.29203238807161824 2225 48-49 0.30035809067724273 2225 50-51 0.26133618201269826 2225 52-53 0.2647317225488308 2225 54-55 0.33445921565840564 2225 56-57 0.3101646821306332 2225 58-59 0.2888845381785998 2225 60-61 0.3022354063278456 2225 62-63 0.287719047343586 2225 64-65 0.3711331105697795 2225 66-67 0.37443351031424044 2225 68-69 0.4315162900178038 2225 70-71 0.30961623287743834 2225 72-73 0.3723716280706384 2225 74-75 0.45726453966484826 2225 76-77 0.46250323287708284 2225 78-79 0.41549396065980204 2225 80-81 0.33937989821994563 2225 82-83 0.4056857821838733 2225 84-85 0.33304045387959036 2225 86-87 0.30471713907007825 2225 88-89 0.2025463612202998 2225 90-91 0.33983447016755264 2225 92-93 0.31524373681685347 2225 94-95 0.633533851763346 2225 96-97 0.45468653460527975 2225 98-99 0.4439616254372112 2225 100 0.37172393890330824 2226 1 1.2595834676661184 2226 2 1.5446587616428147 2226 3 0.9456114959230622 2226 4 0.9307569326940737 2226 5 3.176385359070842 2226 6 -0.515347953250533 2226 7 0.12650729028154473 2226 8 0.3311167547559535 2226 9 0.35426635914725324 2226 10-11 0.13064344391762717 2226 12-13 0.12739948085117447 2226 14-15 0.10848846689989244 2226 16-17 0.07171469251606055 2226 18-19 0.11313643897323544 2226 20-21 0.15789775668707762 2226 22-23 0.23130124175617084 2226 24-25 0.14020271269093598 2226 26-27 0.11771279443181015 2226 28-29 0.12785089835399788 2226 30-31 0.11384522402095598 2226 32-33 0.08071410670422097 2226 34-35 0.16031799368276012 2226 36-37 0.17853916173999806 2226 38-39 0.1266903469200713 2226 40-41 0.17626341972125914 2226 42-43 0.2888455778046648 2226 44-45 0.2338751245614361 2226 46-47 0.1691654183332787 2226 48-49 0.3201190655557298 2226 50-51 0.11390710198687515 2226 52-53 0.22097530031724233 2226 54-55 0.2992864349218749 2226 56-57 0.37545243592463606 2226 58-59 0.3729565482659396 2226 60-61 0.12075267386181565 2226 62-63 0.297711658406115 2226 64-65 0.270757451483135 2226 66-67 0.36653525817801125 2226 68-69 0.43053473557829847 2226 70-71 0.4961555330598699 2226 72-73 0.4775823355449802 2226 74-75 0.48213050280150327 2226 76-77 0.4009947612662188 2226 78-79 0.45481912834304694 2226 80-81 0.26925300416148445 2226 82-83 0.18443223945556042 2226 84-85 0.28407470053221573 2226 86-87 0.5205456591783673 2226 88-89 0.39345752173979065 2226 90-91 0.3720743672308089 2226 92-93 0.33652329396171154 2226 94-95 0.6037985552715242 2226 96-97 0.494316423111556 2226 98-99 0.511354215841962 2226 100 0.6547707106667318 2227 1 1.4310951105400207 2227 2 1.884556098113375 2227 3 0.7283371814927726 2227 4 0.007736437009231878 2227 5 3.193991195745266 2227 6 0.1994670359091657 2227 7 0.2777811697758352 2227 8 0.2991206412303029 2227 9 0.262349965909209 2227 10-11 0.2312271751046282 2227 12-13 0.24201369825689767 2227 14-15 0.1889448236219451 2227 16-17 0.34100927731766717 2227 18-19 0.2423130443198147 2227 20-21 0.26077961223095514 2227 22-23 0.3378168601020306 2227 24-25 0.3169160577484149 2227 26-27 0.19387893900365327 2227 28-29 0.21772868231829534 2227 30-31 0.22947598787300905 2227 32-33 0.07847218814120538 2227 34-35 0.2355078613901398 2227 36-37 0.2581763971651654 2227 38-39 0.18098949560761923 2227 40-41 0.16981446377606346 2227 42-43 0.37298393501657756 2227 44-45 0.3087375031121766 2227 46-47 0.29512186687242803 2227 48-49 0.19395354617373783 2227 50-51 0.1695383954115144 2227 52-53 0.42511443717767605 2227 54-55 0.3188263148020596 2227 56-57 0.37352593177300975 2227 58-59 0.5184748809164645 2227 60-61 0.4025014696358298 2227 62-63 0.3783881187331559 2227 64-65 0.38948934089759035 2227 66-67 0.35198267713685283 2227 68-69 0.38070146513170755 2227 70-71 0.571585118171626 2227 72-73 0.5166320616750468 2227 74-75 0.36719799020760746 2227 76-77 0.5691818038509666 2227 78-79 0.3594866854762131 2227 80-81 0.28764198306954114 2227 82-83 0.22844560527916258 2227 84-85 0.3373312531961261 2227 86-87 0.25645731225144175 2227 88-89 0.2848855804076038 2227 90-91 0.279764901194433 2227 92-93 0.37691668793600996 2227 94-95 0.6375996286857344 2227 96-97 0.5139519202637501 2227 98-99 0.6828186630110906 2227 100 0.6537276242980994 2228 1 1.4164752101808844 2228 2 1.7945291634760068 2228 3 -0.24346804096970587 2228 4 -0.025703830948831552 2228 5 3.1336873643173817 2228 6 0.2785411278394605 2228 7 0.40159402841767644 2228 8 0.4674327475549589 2228 9 0.2315337461828335 2228 10-11 0.22180656713377545 2228 12-13 0.2695986617687538 2228 14-15 0.20592562111369972 2228 16-17 0.09916003168414989 2228 18-19 0.15244910581673565 2228 20-21 0.20711858401438832 2228 22-23 0.19256248781364604 2228 24-25 0.38288483403835016 2228 26-27 0.284462619157857 2228 28-29 0.21490313959210994 2228 30-31 0.30201051142559976 2228 32-33 0.1481815243370761 2228 34-35 0.26707722749864615 2228 36-37 0.3117068270982699 2228 38-39 0.3274219177183255 2228 40-41 0.2793649829827203 2228 42-43 0.542127783606432 2228 44-45 0.4233007457307352 2228 46-47 0.3124041361387313 2228 48-49 0.389351084979495 2228 50-51 0.3585903975750071 2228 52-53 0.3291215005014365 2228 54-55 0.35318722662328383 2228 56-57 0.6052361308059773 2228 58-59 0.5194943174947397 2228 60-61 0.2836659159145043 2228 62-63 0.5411266101518208 2228 64-65 0.4506855050868168 2228 66-67 0.46259675796540023 2228 68-69 0.4470054732966773 2228 70-71 0.5705811401656788 2228 72-73 0.5501558414606365 2228 74-75 0.5855766216986922 2228 76-77 0.5588629995223755 2228 78-79 0.5282076443862564 2228 80-81 0.42237481274165845 2228 82-83 0.4545931466317228 2228 84-85 0.4959161269179475 2228 86-87 0.5585740231695127 2228 88-89 0.5616871319033692 2228 90-91 0.5531080561504496 2228 92-93 0.5869292703846938 2228 94-95 0.7640114227827581 2228 96-97 0.6222284991170426 2228 98-99 0.7429764375831667 2228 100 0.9863576024618297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 30.0 13 37.0 14 151.0 15 547.0 16 2073.0 17 5096.0 18 8371.0 19 10835.0 20 12536.0 21 14364.0 22 16795.0 23 19387.0 24 21676.0 25 24914.0 26 28184.0 27 31526.0 28 36130.0 29 41206.0 30 47080.0 31 54569.0 32 63815.0 33 75735.0 34 94408.0 35 121264.0 36 170040.0 37 280697.0 38 691339.0 39 1312120.0 40 1640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.970753654200056 18.207592125918463 20.595505562286206 35.226148657595274 2 18.305576356114496 51.76528223865204 13.345851329391095 16.58329007584236 3 41.83548742925376 15.193790178452346 23.485822507935662 19.484899884358235 4 17.530444224423476 16.181028788052338 21.938796164521985 44.3497308230022 5 16.481854285100102 34.63102117797691 26.613641962426627 22.273482574496363 6 17.643889266341656 29.072747613809856 28.40133497982938 24.88202814001911 7 20.0036716652571 25.568158816782333 36.30639889661752 18.121770621343046 8 22.314780963200185 28.478283041456866 32.32640790318101 16.880528092161935 9 21.255521227403175 28.28509696177545 26.595628835438788 23.86375297538258 10-11 19.8215476539785 30.36388713238236 28.256194366033643 21.55837084760549 12-13 24.292600339236763 26.52061075170285 27.32953823317585 21.857250675884536 14-15 21.855901260448164 27.519821500581347 28.530925934352506 22.093351304617983 16-17 20.71071828128408 29.84905376165244 28.286650358614995 21.153577598448486 18-19 21.46122862706394 29.510711377298126 29.573992684913065 19.45406731072487 20-21 22.797965834684057 28.762225154672823 29.98098265687347 18.458826353769656 22-23 21.631427571695543 30.57067720256765 28.48606571653175 19.311829509205054 24-25 22.441406341938734 28.322817830485175 26.509721282948878 22.726054544627207 26-27 20.13789142854453 26.671635444436248 30.038693703094083 23.151779423925138 28-29 19.493449822770987 27.989772654167272 30.687827450020478 21.828950073041263 30-31 20.125266437944184 30.066675590431817 28.20566255268539 21.602395418938606 32-33 20.75055741841136 29.89328634438651 28.860559881879077 20.495596355323052 34-35 21.431196288166095 28.918051255819353 30.164471774465607 19.48628068154894 36-37 21.68647116879775 28.310908454715346 28.651070980821043 21.351549395665867 38-39 21.64501098056888 29.149252260603824 29.070703063952163 20.13503369487513 40-41 20.985581736028777 29.816689962761817 29.798315651523893 19.39941264968552 42-43 20.597954880205513 29.878255919429144 29.31619239107734 20.207596809288006 44-45 20.452885787674187 30.263230155355377 28.77446403886316 20.50942001810727 46-47 19.821468668047807 28.302860238955002 28.859536930729156 23.01613416226803 48-49 20.89339147326353 28.011102864683444 30.215482816135868 20.880022845917157 50-51 21.801061644749126 30.857020019990177 28.096806435770176 19.245111899490517 52-53 20.432863929031065 30.490086210007718 28.166109685139375 20.910940175821843 54-55 22.30118694525011 28.6069326541851 28.01839576026189 21.073484640302905 56-57 21.38346945452919 28.706834254364733 29.38546556744453 20.52423072366155 58-59 21.295855722392407 27.97660447948576 28.836705293024657 21.890834505097175 60-61 19.59287739421699 30.460338324245424 29.570240119552178 20.376544161985414 62-63 20.41524478715668 31.128498153138974 27.461186730219566 20.995070329484776 64-65 19.76516065985232 30.368517915000226 28.550461053395644 21.315860371751807 66-67 20.258397365187903 30.562643473458095 29.169136672247387 20.009822489106607 68-69 20.78804292396358 30.06235554586208 29.114375510934188 20.035226019240156 70-71 19.526386563588062 31.115731202721424 29.13893173370071 20.218950499989802 72-73 20.09143074125273 30.808943172350162 28.662101667469514 20.43752441892759 74-75 19.981108183890804 29.39869420520215 30.185968276184543 20.4342293347225 76-77 19.386219957854305 30.786693508527208 28.60746603317365 21.219620500444837 78-79 19.32777144040683 29.59402993505546 28.627974009631064 22.45022461490665 80-81 20.909232270006825 29.60838761214496 28.320006251262136 21.16237386658608 82-83 20.25409806484412 28.327807529424316 29.746639406382734 21.67145499934883 84-85 20.67222855959317 29.03612510650183 28.48520271828756 21.806443615617443 86-87 21.14157407741565 28.18247862510258 29.397423244151614 21.278524053330152 88-89 21.286981436123224 27.458705534015472 28.91583884213879 22.338474187722515 90-91 20.5853952453504 27.815265026760795 29.17779803644363 22.421541691445178 92-93 20.95336200579621 28.294480106321384 29.24448426440383 21.507673623478574 94-95 20.63865008245556 28.465902939372018 29.2707351019044 21.62471187626802 96-97 20.295663198459785 29.14156780106478 29.18923668589845 21.37353231457698 98-99 22.05183324363382 28.73740218699446 28.34238435431256 20.86838021505916 100 20.741393946145774 30.49274061567864 27.990547815594535 20.775317622581056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 12.0 2 16.5 3 19.5 4 25.5 5 30.0 6 39.5 7 52.5 8 60.0 9 64.0 10 80.5 11 97.5 12 119.0 13 143.0 14 159.5 15 190.5 16 245.5 17 340.0 18 512.5 19 806.5 20 1297.5 21 2051.5 22 3149.5 23 4864.5 24 7447.5 25 11173.5 26 16243.5 27 21970.0 28 28506.0 29 36127.5 30 44707.0 31 54594.0 32 66182.0 33 79316.5 34 92877.0 35 108117.5 36 123587.0 37 134514.0 38 142247.0 39 149486.5 40 154893.0 41 157010.0 42 155681.5 43 153120.0 44 150597.0 45 145648.5 46 135702.0 47 124690.0 48 116921.0 49 112249.0 50 108770.0 51 100526.0 52 86954.5 53 72837.5 54 60647.5 55 49773.0 56 38837.0 57 29021.5 58 21086.5 59 15106.0 60 11531.5 61 9480.0 62 7711.5 63 5971.5 64 4506.0 65 3731.5 66 3771.0 67 3475.5 68 2493.0 69 2266.5 70 2887.0 71 2794.0 72 1709.0 73 775.0 74 384.5 75 229.5 76 165.0 77 140.0 78 106.5 79 81.5 80 70.5 81 53.5 82 36.5 83 29.0 84 28.0 85 27.0 86 24.5 87 22.5 88 20.0 89 21.5 90 20.0 91 15.5 92 15.5 93 21.0 94 19.0 95 14.0 96 21.0 97 24.0 98 49.5 99 114.0 100 118.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.36041474126528 2 0.006872604199192547 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.1381754334212545E-4 30-31 0.00993232524677827 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.473073670237387E-4 40-41 0.00160046947104484 42-43 1.098361401697439E-4 44-45 0.0 46-47 0.0020555049088909214 48-49 0.0 50-51 0.0 52-53 0.0013807971907053521 54-55 0.019252706284039398 56-57 0.011689703489494174 58-59 0.015220150852093085 60-61 2.0398140317238157E-4 62-63 0.010920850508305966 64-65 1.098361401697439E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.6674491184080666E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3186565.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.46703057838401 #Duplication Level Percentage of deduplicated Percentage of total 1 85.10617649885978 61.67391894752219 2 9.02782123590831 13.084387951175039 3 2.6555829012872674 5.7732662193305435 4 1.1447356782901188 3.318223816112688 5 0.6185364175461427 2.2411748742080206 6 0.36455744445376337 1.5851037284885037 7 0.2378337098202711 1.2064571905481265 8 0.16812614793083047 0.9746882162503525 9 0.12015398043338547 0.7836481956762629 >10 0.5202691261671081 6.394838908331072 >50 0.02496470288166206 1.2335569343025143 >100 0.010361987227529098 1.2832636436218765 >500 7.041353564957488E-4 0.3006423391230101 >1k 1.7603383718600252E-4 0.1468290353097875 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.138175433421254E-5 0.0 5 0.0 0.0 0.0 3.138175433421254E-5 0.0 6 0.0 0.0 0.0 3.138175433421254E-5 0.0 7 0.0 0.0 0.0 3.138175433421254E-5 0.0 8 0.0 0.0 0.0 3.138175433421254E-5 0.0 9 3.138175433421254E-5 0.0 0.0 3.138175433421254E-5 0.0 10-11 3.138175433421254E-5 0.0 0.0 3.138175433421254E-5 0.0 12-13 3.138175433421254E-5 0.0 0.0 3.138175433421254E-5 0.0 14-15 3.138175433421254E-5 0.0 0.0 3.138175433421254E-5 0.0 16-17 3.138175433421254E-5 0.0 0.0 3.138175433421254E-5 0.0 18-19 3.138175433421254E-5 0.0 0.0 3.138175433421254E-5 0.0 20-21 3.138175433421254E-5 0.0 0.0 4.7072631501318813E-5 0.0 22-23 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 24-25 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 26-27 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 28-29 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 30-31 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 32-33 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 34-35 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 36-37 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 38-39 3.138175433421254E-5 0.0 0.0 6.276350866842508E-5 0.0 40-41 6.276350866842508E-5 0.0 0.0 6.276350866842508E-5 0.0 42-43 7.845438583553136E-5 0.0 0.0 6.276350866842508E-5 0.0 44-45 9.414526300263764E-5 0.0 0.0 6.276350866842508E-5 0.0 46-47 9.414526300263764E-5 0.0 0.0 6.276350866842508E-5 0.0 48-49 9.414526300263764E-5 0.0 0.0 6.276350866842508E-5 0.0 50-51 9.414526300263764E-5 0.0 0.0 6.276350866842508E-5 0.0 52-53 9.414526300263764E-5 0.0 0.0 6.276350866842508E-5 0.0 54-55 9.414526300263764E-5 0.0 0.0 6.276350866842508E-5 0.0 56-57 9.414526300263764E-5 0.0 0.0 6.276350866842508E-5 0.0 58-59 1.8829052600527528E-4 0.0 0.0 6.276350866842508E-5 0.0 60-61 2.8243578900791287E-4 0.0 0.0 7.845438583553136E-5 0.0 62-63 3.7658105201055056E-4 0.0 0.0 9.414526300263764E-5 0.0 64-65 4.550354378460819E-4 0.0 0.0 9.414526300263764E-5 0.0 66-67 5.334898236816133E-4 0.0 0.0 1.2552701733685017E-4 0.0 68-69 5.491807008487196E-4 0.0 0.0 1.5690877167106272E-4 0.0 70-71 6.276350866842509E-4 0.0 0.0 1.5690877167106272E-4 0.0 72-73 6.747077181855697E-4 0.0 0.0 1.5690877167106272E-4 0.0 74-75 7.845438583553137E-4 0.0 0.0 1.5690877167106272E-4 0.0 76-77 9.571435071934826E-4 0.0 0.0 1.5690877167106272E-4 0.0 78-79 0.0011140522788645455 0.0 0.0 1.5690877167106272E-4 0.0 80-81 0.0013651063135382458 0.0 0.0 1.5690877167106272E-4 0.0 82-83 0.0015847785938777337 0.0 0.0 1.5690877167106272E-4 0.0 84-85 0.0016004694710448398 0.0 0.0 1.5690877167106272E-4 0.0 86-87 0.0016318512253790524 0.0 0.0 1.5690877167106272E-4 0.0 88 0.0016318512253790524 0.0 0.0 1.5690877167106272E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 12730 0.0 82.33576 8 CACCGGC 12670 0.0 79.57668 9 CTTCACC 14440 0.0 75.64102 6 CCTTCAC 16135 0.0 71.59305 5 TTCACCG 15215 0.0 70.58497 7 CGTAGTG 3025 0.0 69.515366 4 CGGGCCC 1055 0.0 68.96162 7 CAGCTGC 110 0.0 67.42836 1 GCGTAGT 1950 0.0 65.95445 3 GGGCCCG 1125 0.0 64.25344 8 GTGTGGC 4760 0.0 64.19515 7 AACGGGC 1120 0.0 62.86392 5 CAGCGTA 1315 0.0 62.04435 1 GGCCCGA 1170 0.0 61.782158 9 GCCTTCA 4850 0.0 60.87474 4 ACGGGCC 1160 0.0 60.696194 6 GCAGCCT 35 1.6258385E-4 60.54792 1 ACCTTCA 6300 0.0 60.27485 4 CGGGGCA 1295 0.0 58.355835 94 TGTGGCA 5585 0.0 57.738 8 >>END_MODULE